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| 1 | Budesonide/formoterol maintenance and reliever therapy in Chinese patients with asthma显示文摘 | LIN Jiang-tao CHEN Ping ZHOU Xin SUN Tie-ying XIE Can-mao XIU Qing-yu YAO Wan-zhen YANG Lan YIN Kai-sheng ZHANG Yong-ming | 2012 | Chinese Medical Journal2012，，17： | 8 |
| 2 | The molecular characterization of maize B chromosome specific AFLPs显示文摘The origin and evolution of B chromosomes could be explained by the specific DNA sequence on them. But the specific sequences known were quite limited. To investigate maize B chromosome sqicific DNA sequeces, maize genomes with and without B chromosomes were analyzed by AFLP. Only 5 markers were found specific to genomes with B chromosomes among about 2000 AFLP markers. Southern hybridization and sequence analysis revealed that only the sequence of M8-2D was a B chromosome specific sequence. This sequence contained the telomeric repeat unit AGGGTTT conserved in plant chromosome telomeres. In addition, the sequence of M8-2D shared low homology to clones from maize chromosome 4 centromere as well. M8-2D were localized to B chromosome centrorneric and telomeric regions. | ZHONG XIA QI, Hui ZENG, Xiu LAN LI, CHENG BIN CHEN, WEN QIN SONG, RUI YANG CHEN The College of Life Sciences, Nankai University, Tianjin 300071, China | 2002 | Cell Research2002，12，1： | 7 |
| 3 | Grade classification of neuroepithelial tumors using high-resolution magic-angle spinning proton nuclear magnetic resonance spectroscopy and pattern recognition显示文摘Clinical data have shown that survival rates vary considerably among brain tumor patients,according to the type and grade of the tumor.Metabolite profiles of intact tumor tissues measured with high-resolution magic-angle spinning proton nuclear magnetic resonance spectroscopy (HRMAS 1H NMRS) can provide important information on tumor biology and metabolism.These metabolic fingerprints can then be used for tumor classification and grading,with great potential value for tumor diagnosis.We studied the metabolic characteristics of 30 neuroepithelial tumor biopsies,including two astrocytomas (grade I),12 astrocytomas (grade II),eight anaplastic astrocytomas (grade III),three glioblastomas (grade IV) and five medulloblastomas (grade IV) from 30 patients using HRMAS 1H NMRS.The results were correlated with pathological features using multivariate data analysis,including principal component analysis (PCA).There were significant differences in the levels of N-acetyl-aspartate (NAA),creatine,myo-inositol,glycine and lactate between tumors of different grades (P<0.05).There were also significant differences in the ratios of NAA/creatine,lactate/creatine,myo-inositol/creatine,glycine/creatine,scyllo-inositol/creatine and alanine/creatine (P<0.05).A soft independent modeling of class analogy model produced a predictive accuracy of 87% for high-grade (grade III-IV) brain tumors with a sensitivity of 87% and a specificity of 93%.HRMAS 1H NMR spectroscopy in conjunction with pattern recognition thus provides a potentially useful tool for the rapid and accurate classification of human brain tumor grades. | CHEN WenXue LOU HaiYan ZHANG HongPing NIE Xiu LAN WenXian YANG YongXia XIANG Yun QI JianPin LEI Hao TANG HuiRu CHEN FenEr DENG Feng | 2011 | Science China(Life Sciences)2011，54，7： | 5 |
| 4 | Utility of Droplet Digital PCR Assay for Quantitative Detection of Norovirus in Shellfish, from Production to Consumption in Guangxi, China显示文摘Objective Shellfish are recognized as important vehicles of norovirus-associated gastroenteritis. The present study aimed to monitor norovirus contamination in oysters along the farm-to-fork continuum in Guangxi, a major oyster production area in Southwestern China. Methods Oyster samples were collected monthly from farms, markets, and restaurants, from January to December 2016. Norovirus was detected and quantified by one-step reverse transcription-droplet digital polymerase chain reaction(RT-ddPCR). Results A total of 480 oyster samples were collected and tested for norovirus genogroups I and II. Norovirus was detected in 20.7% of samples, with genogroup II predominating. No significant difference was observed in norovirus prevalence among different sampling sites. The norovirus levels varied widely, with a geometric mean of 19,300 copies/g in digestive glands. Both norovirus prevalence and viral loads showed obvious seasonality, with a strong winter bias. Conclusion This study provides a systematic analysis of norovirus contamination ‘from the farm to the fork' in Guangxi. RT-ddPCR can be a useful tool for detection and quantification of low amounts of norovirus in the presence of inhibitors found particularly in foodstuffs. This approach will contribute to the development of strategies for controlling and reducing the risk of human illness resulting from shellfish consumption. | TAN Dong Mei LYU Su Ling LIU Wei ZENG Xian Ying LAN Lan QU Cong ZHUGE Shi Yang ZHONG Yan Xu XIE Yi Hong LI Xiu Gui | 2018 | Biomedical and Environmental Sciences2018，31，10： | 4 |
| 5 | Development of Selenium-rich Passion Fruit Industry in Guangxi显示文摘According to the understanding of current situation of Guangxi selenium-containing passion fruit products and the advantages and disadvantages of related product markets,the corresponding improvement plans were proposed for the supply and demand of products and the adjustment of industrial development,so as to provide theoretical data support for optimizing the industrial structure and increasing the market share of selenium-rich passion fruit in Guangxi. | Xinhui SU Jun TAN Xiu LAN Yiyuan YANG Liping PAN Qiong ZHOU Yongxian LIU | 2019 | Agricultural Biotechnology2019，8，3： | 4 |
| 6 | Synthesis of N-methylene phosphonic chitosan(NMPCS)and its potential as gene carder显示文摘N-Methylene phosphoulc chitosan (NMPCS),an amphiphilic macromolecule with powerful chelating ability of Ca^(2+) ions,was synthesized and characterized.The physicochemical properties of NMPCS and the interactions between NMPCS and plasmid DNA were investigated by FTIR,^(13)C NMR,X-ray,agarose gel electrophorcsis retardation assay,atomic force microscopy (AFM) and circular dichroism (CD).The results suggest that at charge ratio 2:1 or above,DNA could be completely entrapped and spherical complexes with mean size of 80-210 nm were formed.Taking HeLa as host cell,luciferase expression mediated by NMPCS improved about 100 times compared to the expression mediated by chitosan. | Dun Wan Zhu Jin Gen Bo Hai Ling Zhang Wen Guang Liu Xi Gang Leng Cun Xian Song Yu Ji Yin Li Ping Song Lan Xia Liu Lin Mei Xiu Lan Li Yang Zhang Kang De Yao | 2007 | Chinese Chemical Letters2007，18，11： | 3 |
| 7 | Construction of a DNA library from chromosome 4 of rice(Oryza sativa)by microdissection显示文摘A simple method to create a chromosome-specific DNA library of rice, including microdissection, amplification,characterization and cloning, is described. Rice chromosome 4 from a metaphase cell has been isolated and amplified by the Linker Adapter PCR (LA-PCR). The PCR products were labeled as probes with DIG-11-dUTP using the random priming method.Southern blot analysis with rice genomic DNA and specific RFLP markers demonstrated that the PCR products were derived from rice chromosome 4. A large library comprising over 100,000recombinant plasmid microclones from rice chromosome 4was constructed. Colony hybridization showed that 58% of the clones contained single or low-copy sequences and 42%contained repetitive sequences. The size of inserts generated by PCR ranged from 140bp to 500bp.This method will facilitate cloning of the specific chromosome DNA markers and important genes of rice. | MAO YING WEI SI YUAN LIANG WEN QIN SONG XIU LAN LI RUI YANG CHEN (Biology Department, Nankai University, Tianjin 300071,China) | 1998 | Cell Research1998，8，4： | 2 |
| 8 | Efficiency and mechanism of new poly (acryl-phenylamidrazone phenylhydrazide) chelating fiber for adsorbing trace Ga, In, Bi, V and Ti from solution 显示文摘 | Chang Xi jun Yang Xiu lan Wei Xin jie | 2001 | Analytica Chimica Acta2001，450，： | 1 |
| 9 | Acrylamide Alters Cytoskeletal Protein Level in Rat Serum显示文摘To study biomarker of acrylamide (ACR) induced neuropathy,Wistar rats received 20 or 40mg/kg of ACR by ip injection and the levels of light neurofilament (NF-L),middle NF (NF-M),heavy NF (NF-H),β-actin,α-tubulin,and β-tubulin proteins in serum were evaluated using both SDS-PAGE and Western blotting.Compared to controls,NF-L and NF-M decreased,howeverα-tubulin,β-tubulin,and β-actin increased (P<0.01).This result suggested that the change of these cytoskeletal | YU Su Fang SONG Fu Ying YI Chao YANG Xi Wei LI Guo Zhen ZHANG Cui Li ZHAO Xiu Lan XIE Ke Qin | 2013 | Biomedical and Environmental Sciences2013，26，11： | 1 |
| 10 | Hydrothermal Synthesis and Structure of [CH3NH3]2[(VⅣO)2(VⅤO4)2]:A New Layered Mixed-valence Vanadium Oxide Incorporated with Organic Cations显示文摘A new layered mixed valence vanadium oxide, [CH 3NH 3] 2[(V ⅣO) 2(V ⅤO 4) 2], which contains interlamellar organic cations was prepared under hydrothermal conditions and its single crystal structure was determined. It crystallizes in a triclinic system with space group P 1, a =0 625 59(8) nm, b =0 639 84(9) nm, c =0 747 19(10) nm, α =78 718(2)°, β =80 099(2)°, γ =77 100(2). The compound contains mixed valence V 4+ /V 5+ vanadium oxide layers constructed from VO 4 tetrahedra, pairs of edge sharing VO 5 square pyramid and methylamine with protonated organic amines occupying the interlayer space. | ZHANG Hong, LI Yang guang, LAN Yang, WANG Xiu liDuan Li ying, WANG En bo ** and HU Chang wenInstitute of Polyoxometalate Chemistry, Department of Chemistry, Northeast Normal University, Changchun 130024, P. R. ChinaYAO Yuan gen, CHEN Jiu tong | 2002 | Chemical Research in Chinese Universities2002，18，3： | 1 |
| 11 | Lyme Borreliosis-associated Risk Factors in Residents of Beijing Suburbs: a Preliminary Case-control Study显示文摘A population-based case-control study was conducted to evaluate the relative factors in the environments,agricultural works,outdoor activities,and the effectiveness of Lyme borreliosis(LB)-associated personal protective measures in Beijing.Thirty-four cases and 272 controls were personally interviewed by well-trained interviewers. | DOU Xiang Feng LYU Yan Ning JIANG Yi LIN Chang Ying TIAN Li Li WANG Quan Yi YANG Yu Song LI Chao SUN Yu Lan GUAN Zeng Zhi ZHANG Xiu Chun LI Xin Yu | 2014 | Biomedical and Environmental Sciences2014，27，10： | 0 |
| 12 | Novel Antifungal Antibiotic, WB Produced by the Fungus Strain 38显示文摘WB, a new antifungal antibiotic, was isolated from the soil fungus strain 38 againstPyricularta oryzae, and its structure was elucidated as 2-[(E)-3,5-dimethyl-3,4-dihydrox-1-eptylenyl]-4-chloro-5-oxo-6-methyl-6,7-dihydroxy-5,6,7,7’-4H-benzo[d]-2H-pyran on the basis of1H-NMR, 13C-NMR, DEPT, HMQC, 1H-13H COSY, HMBC spectra. | Jun YANG Xiu Lan QI Gang SHAO Yue Hu PEI Xin Sheng YAO (Department of Natural Medicinal Chemistry, Shenyang Pharmaceutical University Shenyang 110015)Susumu KITANAKA (Department of Pharmacognosy, College of Pharmacy, Nihon University, Japan) | 1998 | Chinese Chemical Letters1998，9，9： | 0 |
| 13 | WA, a new Antifungal Antibiotic from a Soil Fungus Strain 38显示文摘A new antifungal antibiotics, WA, was isolated from the secondary metabolites of soilfungus strain 38, and its structure was elucidated as 4- (2- hydroxy- 3 -butynoxy)benzoic acid bymeans of the spectral data (H-and C-NMR, DEPT, H-H COSY, H-13C COSY, HMBC). | Jun YANG Xiu Lan QI Wen LI Gang SHAO Xin Sheng YAO S. KITANAKA(Department of Natural Medicinal Chemistry, Shenyang Pharmaceutical University, Shenyang 110015)(Department of Pharmacognosy, College of Pharmacy, Nihon University, Japan) | 1998 | Chinese Chemical Letters1998，9，6： | 0 |
| 14 | Angiotensin IV upregulates the activity of protein phosphatase 1α in Neura-2A cells显示文摘The peptide angiotensin IV(Ang IV)is a derivative of angiotensin II.While insulin regulated amino peptidase(IRAP)has been proposed as a potential receptor for Ang IV,the signalling pathways of Ang IV through IRAP remain elusive.We applied high-resolution mass spectrometry to perform a systemic quantitative phosphoproteome of Neura-2A(N2A)cells treated with and without Ang IV us-ing sta ble-isotope labeling by amino acids in cell culture(SILAC),and identifi ed a reduction in the phosphorylation of a major Ser/Thr protein phosphorylase 1(PP1)upon Ang IV treatment.In addition,spinophilin(spn),a PP1 reg-ulatory protein that plays important functions in the neural system,was expressed at higher levels.Immunoblotting revealed decreased phosphorylation of p70S6 kinase(p70S6K)and the major cell cycle modulator retinoblas-toma protein(pRB).These changes are consistent with an observed decrease in cell proliferation.Taken together,our study suggests that Ang IV functions via regulating the activity of PP1. | Dan Wang Peng Xue Xiu Lan Chen Zhen Sheng Xie Fu Quan Yang Li Zheng Tao Xu | 2013 | Protein & Cell2013，4，7： | 0 |
| 15 | Immunogenicity of Whole Mycobacterium intracellulare Proteins and Fingding on the Cross-Reactive Proteins between M.intracellulare and M.tuberculosis显示文摘Objectives To evaluate the immunogenicity of Mycobacterium intracellulare proteins and determine the cross-reactive proteins between M.intracellulare and M.tuberculosis.Methods Protein extracts from M.intracellulare were used to immunize BALB/c mice.The antigens were evaluated using cellular and humoral immunoassays.The common genes between M.intracellular and M.tuberculosis were identified using genome-wide comparative analysis,and cross-reactive proteins were screened using immunoproteome microarrays.Results Immunization with M.intracellulare proteins induced significantly higher levels of the cytokines interferon-γ(IFN-γ),interleukin-2(IL-2),interleukin-12(IL-12),interleukin-6(IL-6)and immunoglobulins IgG,IgG1,IgM,and IgG2a in mouse serum.Bone marrow-derived macrophages isolated from mice immunized with M.intracellulare antigens displayed significantly lower bacillary loads than those isolated from mice immunized with adjuvants.Whole-genome sequence analysis revealed 396 common genes between M.intracellulare and M.tuberculosis.Microchip hybridization with M.tuberculosis proteins revealed the presence of 478 proteins in the serum of mice immunized with M.intracellulare protein extracts.Sixty common antigens were found using both microchip and genomic comparative analyses.Conclusion This is the advanced study to investigate the immunogenicity of M.intracellulare proteins and the cross-reactive proteins between M.intracellulare and M.tuberculosis.The results revealed the presence of a number of cross-reactive proteins between M.intracellulare and M.tuberculosis.Therefore,this study provides a new way of identifying immunogenic proteins for use in tuberculosis vaccines against both M.intracellulare and M.tuberculosis in future. | XIAO Shi Qi XU Da DUAN Hong Yang FAN Xue Ting LI Gui Lian ZHANG Wen LI Ma Chao HAN Na LI Xin Yao LI Na ZHAO Li lan ZHAO Xiu Qin WAN Kang Lin LIU Hai Can FENG Wen Hai | 2021 | Biomedical and Environmental Sciences2021，34，7： | 0 |
| 16 | Isolation and Structure Elucidation of an Antifungal Antibiotics A73 Produced by a Soil Fungus Strain No.131显示文摘IntroductionMorphologicaldeformationofmyceliaorconidiaofmicroorganisms,suchascurling,sweling,hyper-divergency,beadformation,a... | YANG Jun, QI Xiu lan , SHAO Gang and YAO Xin sheng * (Department of Natural Products Chemistry, Department of Microbiology Shenyang Pharmaceutical University , Shenyang, 110015) Susumu Kitanaka (Department of Pharmacognosy, College of Pharm | 1999 | Chemical Research in Chinese Universities1999，15，1： | 0 |
| 17 | A new lavandulyl flavonoid from Sorphora flavescens Ait显示文摘新 lavandulyl flavonoid 从 Sorphora flavescens 的根茎被孤立。新化合物的结构被阐明根据光谱方法包括 2D NMR。并且它显示出有势力对有 1.1 mol/L 的 IC50 的 Hela 房间的细胞毒素的活动。 | Dan Liu Rong Lan Xiu Lan Xin Xiao Jie Wang Dong Hai Su Guo Wei Yang | 2008 | Chinese Chemical Letters2008，19，12： | 0 |
| 18 | Muricatenol, a Linear Acetogenin from Annona muricata (Annonaceae)显示文摘Muricatenol 1. a new acetogenin. has been isolated from the seeds of .Annona, muricata L.. Compound 1 is a C37, acetogenin without any THF rings. with four hydroxyls and one double bond in the long aliphatic chain. The hydroxyls of I are located at C-4. C-10. C-18 and C-19. respectively. The vicinal diol at C-18/C-19 is threo-configuration. and the double bond at 14/C-15 is cis-configuration. | De Yu LI Jing Guang YU Xiu Zhen LUO Lan SUN Shi Lin YANG (Institute of Medicinal Plant Development. Chinese Academy of Medical Sciences and Peking Union Medical College. Beijing 100094) | 2000 | Chinese Chemical Letters2000，11，3： | 0 |
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