| 1 | Expression of IGF-II in early experimental hepatocellular carcinomas and its significance in early diagnosis显示文摘AIM: To investigate the serum level and expression of insulin growth factor II (IGF-II) in liver tissues of rats with early experimental hepatocellular carcinomas (HCC) and its significance in early diagnosis.METHODS: Early experimental hepatocellular carcinomas were induced by diethylnitrosamine (DENA) in 180 male SD rats. Another 20 male SD rats served as control. The IGF-IIserum level was measured by ELISA. Immunohistochemistry and electron microscopic immunohistochemistry were usedto observe the expression of IGF-II in normal and tumor liver tissues and its ultrastructural location in malignant hepatocytes. The expressions of IGF-II in human hepatoma cell lines HepG2, SMMC7721 and human embryonic liver cell line L-02 were measured by immunocytochemistry. IGF-II mRNA level was studied by in situ hybridization.RESULTS: IGF-II was expressed in the cytoplasm of both sinusoidal cells in paracancerous cirrhotic liver tissue and malignant hepatocytes in early experimental HCC tissues.Gold particles were seen on the rough endoplasrnic reticulum and the mitochondrion in malignant hepatocytes. IGF-II was expressed in the human hepatoma cell lines. The mRNA level of IGF-II was higher in rat liver tumor tissue than in normal rat liver tissue. The serum IGF-II level of the early experimental HCC group was 34.67±10.53 ng.ml^-1 and that of the control group was 11.75±5.84 ng.ml^-1. The rank sum test was used for statistical analysis. There was a significant difference between the two groups (P<0.01).CONCLUSION: During the induction of early experimental HCC by DENA, IGF-II may promote hepatocytic proliferation via a paracrine mechanism in the pre-cancerous stage. When hepatocytes are transformed into malignant cells, they may secrete IGF-II and promote malignant cell proliferation by an autocrine mechanism. IGF-II may be a possible biological marker in the early diagnosis of HCC. | ZhengWang You-BingRuan YangGuan Sheng-HongLiu1 | 2003 | World Journal of Gastroenterology2003，9，2： | 24 |
| 2 | Coordinated flight control of miniature fixed-wing UAV swarms: methods and experiments显示文摘In this paper,we present our recent advances in both theoretical methods and field experiments for the coordinated control of miniature fixed-wing unmanned aerial vehicle(UAV)swarms.We propose a multi-layered group-based architecture,which is modularized,mission-oriented,and can implement large-scale swarms.To accomplish the desired coordinated formation flight,we present a novel distributed coordinated-control scheme comprising a consensus-based circling rendezvous,a coordinated path-following control for the leader UAVs,and a leader-follower coordinated control for the follower UAVs.The current framework embeds a formation pattern reconfiguration technique.Moreover,we discuss two security solutions(inter-UAV collision avoidance and obstacle avoidance)in the swarm flight problem.The effectiveness of the proposed coordinated control methods was demonstrated in field experiments by deploying up to 21 fixed-wing UAVs. | Xiangke WANG Lincheng SHEN Zhihong LIU Shulong ZHAO Yirui CONG Zhongkui LI Shengde JIA Hao CHEN Yangguan YU Yuan CHANG Yajing WANG | 2019 | Science China(Information Sciences)2019，62，11： | 21 |
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