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| 1 | Generation of human neutralizing monoclonal antibodies against the 2009 pandemic H 1N 1 virus from peripheral blood memory B lymphocytes显示文摘2009 H1N1 流行性感冒流行表明了对人的全球健康威胁的意义。尽管流行 H1N1 疫苗很快被开发了,被动 serotherapy 可以在孩子对感染提供优异立即的保护,老、妥协免疫者的病人在流行性感冒期间流行。这里,我们基于使不朽的 Epstein-Barr 病毒(EBV ) 使用了新奇策略从个人屏蔽高病毒的抵销 monoclonal 抗体(MAbs ) 的外部血记忆 B 房间与 2009 流行 H1N1 疫苗的 PANFLU.1 种牛痘。通过 13 090 使不朽的记忆 B 房间的一幅巨大的屏幕,从三的克隆选择了 vaccinees,七 MAbs 与两个高病毒的抵销能力和红血球凝聚抑制(HAI ) 被识别对 2009 个流行 H1N1 病毒的活动。这些 MAbs 可以与严重呼吸症候群为感染的病人的被动 serotherapy 治疗有重要临床的含意,特别孩子,老并且 immunodeficient 个人。我们为从使 EBV 不朽的外部血记忆 B 房间产生高亲密关系的 MAbs 的成功的策略可能也对另外的传染或自体免疫的疾病适用。 | Hao Wang Chi Ma Yanlai Lu Xu Ji Yongsheng Pang Fang Hua Lianxian Cui Denian Ba Wei He | 2013 | Cellular & Molecular Immunology2013，10，5： | 3 |
| 2 | A novel recombinant DNA vaccine encodingMycobacterium tuberculosis ESAT-6 and FL protects againstMycobacterium tuberculosis challenge in mice显示文摘Mycobacterium tuberculosis 6-kDa early secretory antigenic target(ESAT-6)is a dominant target antigen for cell-mediated immunity in the early phase of tuberculosis.The fms-like tyrosine kinase 3 ligand(FL)that induces potent immune response has been used as an adjuvant in vaccine development.In this study,a new recombinant plasmid(pIRES-epitope-peptides-FL)encoding three T cell epitopes of ESAT-6 and FL was constructed,and the immunogenicity of the DNA vaccine was assessed in C57BL/6 mice immunized with the plasmid DNA vaccine.Additionally,a strategy of intramuscular injection with the DNA vaccine(prime)and intranasal administration of the epitope peptides(boost)was employed to induce higher immune reaction of the mice.The results showed that mice vaccinated with the recombinant plasmid DNA vaccine and boosted with the peptides not only increased the levels of Th1 cytokines(IFN-γand IL-12),the number of IFN-γ+T cells and activities of cytotoxic T lymphocytes as well as IgG,but also enhanced protection against Mycobacterium tuberculosis challenge.In conclusion,these data indicate that the novel recombinant pIRES-epitope-peptides-FL plasmid is a useful DNA vaccine for preventing Mycobacterium tuberculosis infection. | Qingtao Jiang Jing Zhang Xia Chen Mei Xia Yanlai Lu Wen Qiu Ganzhu Feng Dan Zhao Yan Li Fengxia He Guangyong Peng Yingwei Wang | 2013 | The Journal of Biomedical Research2013，27，5： | 2 |
| 3 | The interaction of influenza H5N1 viral hemagglutinin with sialic acid receptors leads to the activation of human γδT cells显示文摘高度病原的鸟的流行性感冒 H5N1 流行病是重要公共健康危险。有哺乳动物的传播活动的遗传上设计的 H5N1 病毒加亮人的流行性感冒 H5N1 的潜在的风险流行。响应流行性感冒 H5N1 病毒理解天生的免疫系统的内在的原则将导致这些潜在地致命的病毒的改进预防和控制。γ δ当第一对微生物引起的感染防卫排队并且帮助在病毒的感染的早阶段期间开始适应有免疫力的回答, T 房间行动。在这研究,我们调查了 γ 的分子的机制; δ响应流行性感冒 H5N1 病毒的感染的 T 房间。我们发现从流行性感冒 H5N1 病毒的三不同紧张导出的 recombinant 红血球凝聚素(rHA ) 得到了 γ 的激活; δ在外部血 mononuclear 房间(PBMC ) 有教养的 T 房间。两 CD69 的房间表面表示, γ 上的一个早激活标记; δinterferon-γ 的 T 房间,和生产;(IFN-γ) 显著地被增加。尤其是, rHA 导致蛋白质的 γ δT 房间激活没被 TCRγ 调停; δ, NKG2D 或模式识别受体(PRR ) 或 NKp46 受体。有 sialic 酸受体的 rHA 蛋白质的相互作用可以在 γ 起一个关键作用; δT 房间激活。我们的数据可以提供卓见进位于 γ 下面的机制; δ响应有 H5N1 病毒的感染的 T 房间激活。 | Yanlai Lu Zhen Li Chi Ma Hao Wang, Jing Zheng Lianxian Cui Wei He | 2013 | Cellular & Molecular Immunology2013，10，6： | 2 |
| 4 | Sublytic C5b-9 triggers glomerular mesangial cell apoptosis in rat Thy-1 nephritis via Gadd45 activation mediated by Egr-1 and p300-dependent ATF3 acetylation显示文摘The apoptosis of glomerular mesangial cells(GMCs)is considered to be an important contributor to the initiation and development of rat Thy-1 nephritis(Thy-1N)and is accompanied by sublytic C5b-9 deposition.However,the mechanism by which sublytic C5b-9 triggers GMC apoptosis has not been elucidated.In this study,functional and histological examinations were performed on GMCs treated with sublytic C5b-9(in vitro)and renal tissues of Thy-1N rats(in vivo).The in vitro studies found that sublytic C5b-9 could trigger GMC apoptosis through upregulating Egr-1,ATF3,and Gadd45 expression.Egr-1-mediated post-transcriptional modulation of ATF3,Egr-1/ATF3-enhanced Gadd45 promoter activity,and p300-mediated ATF3 acetylation were all involved in GMC apoptosis.More importantly,the effective binding elements for Egr-1 and ATF3 to Gadd45β/γpromoters and the ATF3 acetylation site were identified.In vivo,silencing renal p300,Egr-1,ATF3,and Gadd45β/γsignificantly decreased GMC apoptosis,secondary GMC proliferation,and urinary protein secretion in Thy-1N rats.Together,these findings implicate that sublytic C5b-9-induced activation of Egr-1/p300–ATF3/Gadd45 axis plays a critical role in GMC apoptosis in Thy-1N rats. | Fengxia He Mengya Zhou Tianyi Yu Dan Zhao Jing Zhang Wen Qiu Yanlai Lu Yu Liu Lulu Wang Yingwei Wang | 2016 | Journal of Molecular Cell Biology2016，8，6： | 1 |
| 5 | The effect oftetrahydrofuran on the enzymatic activity and microbial community in activated sludge from a sequencing batehreaetor显示文摘 | Yanlai Yao Zhenmei Lu Hang Min | 2012 | Eeotoxicology2012，21，1： | 1 |
| 6 | Author Correction:A novel recombinant DNA vaccine encoding Mycobacterium tuberculosis ESAT-6 and FL protects against Mycobacterium tuberculosis challenge in mice显示文摘Correction to:Journal of Biomedical Research https://doi.org/10.7555/JBR.27.20120114,published on 30 September 2013.We apologize for the misused images in Fig.7 in our article published on 30 September 2013.The Fig.7 by HE staining and immunohistochemistry were partially misused because of our carelessness.Because we couldn't find the original pictures acquired 11 years ago,thus HE and IHC experiments were repeated by our previously reserved wax blocks.Now,the pictures taken by microscope imaging system in each group were collected and combined,respectively.The correct version of Fig. | Qingtao Jiang Jing Zhang Xia Chen Mei Xia Yanlai Lu Wen Qiu Ganzhu Feng Dan Zhao Yan Li Fengxia He Guangyong Peng Yingwei Wang | 2022 | The Journal of Biomedical Research2022，36，6： | 0 |
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