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| 1 | Deletion of cagA gene of Helicobacter pylori by PCR products显示文摘AIM: Cytotoxin-associated protein (antigen) A (CagA)plays an important role in Helicobacter pylori(H pylori)pathogenesis. Our aim was to obtain cagA mutant strains by a new mutation method so as to better understand the mechanism of CagA in epithelial cells. METHODS: In contrast with the traditional method using suicide plasmid, we constructed cagA- mutant strains directly with PCR products. The constructed mutant clones grew on selective media and allelic exchange was confirmed by Southern blot. Furthermore, two different transformation methods, electroporation, and natural transformation, were compared with regard to the efficiency of recombination.RESULTS: The mutation by PCR products could be completed within 3-5 d, and the recombination rate by electroporation and natural transformation was 4.02×10-8 and 1.03x 10-9 respectively. Mutation rate by electroporation (4.02× 10-8) was far higher than by natural transformation (1.03× 10-9) (P = 0.000<0.005).CONCLUSION: cagA- mutant strains have been constructed,which is important for further study on the function of CagA in epithelial cells. A mutation method by directly using PCR products has been proved successful with a much higher mutation rate, and is easier, especially when in combination with electroporation. This method could be widely used in gene deletion of H pylori. | XunZeng Li-HuaHe YanYin Mao-JunZhang Jian-ZhongZhang | 2005 | World Journal of Gastroenterology2005，11，21： | 5 |
| 2 | Thermal reversible gelation during phase separation of poly(N-isopropyl acrylamide)/water solution显示文摘 | Fang Zeng Xinxing Liu Zhen Tong Yanyin Yang Shuizhu Wu | 2000 | Science in China Series B: Chemistry2000，，4： | 1 |
| 3 | Compressior,strength and deformation of gellan gels formed with mono and divalent cations显示文摘 | Juming Tang Marvin A Tung Yanyin Zeng | 1996 | Carbohydrate Polymers1996，29，1： | 1 |
| 4 | Effect of tamoxifen,methoxyprogesterone acetate and combined treatment on cellular proliferation and apoptosis in SK0V3/DDP cells via the regulation of vascular endothelial growth factor显示文摘 | Wen L Hong D Yanyin W | 2013 | Arch Gynecol Obstet2013，287，5： | 1 |
| 5 | Compression strength and deformation of gellan gels formed with mono-and divalent cations显示文摘 | TANG Juming TUNG M A ZENG Yanyin | 1996 | Carbohydrate Polymers1996，29，1： | 1 |
| 6 | Compressior,strength and deformation of gellan gels formed with mono and divalent cations显示文摘 | Juming Tang Marvin A Tung Yanyin Zeng | | 0，，01： | 1 |
| 7 | Camosine protects against NMDA-inducedneurotoxicity in differentiated rat PC12 cells throughcamosine-histidine-histamine pathway and H1/H3receptors 显示文摘 | SHEN Yao HU Weiwei FAN Yanyin | 2007 | Biochemical Pharmacology2007，73，： | 1 |
| 8 | Healing and Destruction in Jhumpa Lahiri’s“Hell-Heaven”——A Structuralist Perspective显示文摘The short story'Hell-Heaven',taken from Jhumpa Lahiri’scollection Unaccustomed Earthi,presents contrasting images and associations in its title,and a search through the whole text ren-ders a display of many binary oppositions consistent with the title.According to the structuralist theory,binary oppositions are two diametrically opposing ideas that are the most basic building blocks of structure in a textii.A structuralist reading of'Hell-Heaven'yields evidence of the binary opposition | 王欷燕 Li Yanyin | 2012 | 校园英语2012，，24： | 0 |
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