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| 1 | Apoptosis in suspension culture of tobacco cells induced by heat shock显示文摘The induction of apoptosis in suspension culture of tobacco cells by heat shock is reported for the first time. Heat treatment (48℃ for 4 h) of tobacco cells led to the appearance of typical hallmarks of apoptosis. It was demonstrated by DNA laddering analysis that the cells treated with heat shock at 48℃ for 4 h had a serious degradation of nuclear DNA into multi-nu-cleosomal sizes, suggesting that heat shock activated endogenous nuclease which led to DNA cleavage at the linkage sites between the nucleosomes, but ladders were very faint for DNA from 2 and 9 h heat-treated cells. The terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labelling (TUNEL) detection also showed that most of these treated cells (48℃ for 4 h) displayed positive reactions, indicating a serious DNA 3’-OH cleavage in their nuclei. Moreover, some other cytological changes in apoptotic cells, such as cell shrinkage, chromatin aggregation, nucleus collapse, have also been observed by 4’, | Haoming Chen Changhui Yan Xiaofang Jiang Yaoren Dai | 1999 | Chinese Science Bulletin1999，44，12： | 2 |
| 2 | Assaying poly(ADP-ribose) polymerase activity in plants by polarographic method显示文摘A new method has been developed to assay poly(ADP-ribose) polymerase (PARP) activity in plant tissues through determining the content of nicotinamide (NIC) produced by enzymatic reaction by linear sweeping polarographic method. The detection limit of NIC was 0.03μmol/L, the calibration graph was linear up to 5μmol/L ( r = 0.999). The recoveries were approximately in the range of 92% to 98% and the relative standard deviations were less | Ruihua Tian Depu Chen Yaoren Dai | 1999 | Chinese Science Bulletin1999，44，20： | 2 |
| 3 | Menadione-induced apoptosis and its mechanism in plants显示文摘Menadione can induce apoptosis in tobacco protoplasts, Typical characteristics are detected including the condensation of chromatin, the formation of apoptotic bodies and the degradation of genomic DNA into 'DNA ladder'. Specific DNase is activated during this process. Ca2+ and Mg2+ are necessary for its activation, while 2n2+ and EDTA (Ethylenediaminetetraacetic acid) can inhibit its activation. The fragmentation of DNA and lamin can be inhibited by DEVD (Ac-Asp -Glu- Val- Asp-aldehyde). The fragmentation of lamin can also be inhibited by PMSF (Phenylmethylsulfonyl fluoride) and CH (Cyclohexinide). These results show that activation of specific DNase and proteases is involved in menadion-induced apoptosis in plants. | SUN Yingli ZHOU Jun DAI Yaoren ZHAI Zhonghe | 2000 | Chinese Science Bulletin2000，45，4： | 1 |
| 4 | Construction of a cell-free system using extracts from apoptotic plant cells显示文摘To study the mechanism of plant apoptosis, a cell-free system has been developed using the extracts prepared from tobacco cells that become committed to apoptosis induced by heat shock treatment. Tobacco nuclei have displayed such characteristics of apoptosis as nucleus condensation and formation of apoptotic bodies during incubation in this system. Besides, these morphological changes are accompanied by DNA fragmentation, which is detected | Jun Zhou Haoming Chen Xiaofang Jiang Haizhen Zhu Yaoren Dai | 1999 | Chinese Science Bulletin1999，44，16： | 1 |
| 5 | The changes in telomerase activity and telomere length in HeLa cells undergoing apop-tosis induced by sodium butyrate显示文摘The changes in telomerase activity and telomere length during apoptosis in HeLa cells as induced by sodium butyrate (SB) have been studied. After a 48 h SB treatment, HeLa cells demonstrated characteristic apoptotic hallmarks including chromatin condensation, formation of apoptotic bodies and DNA Laddering which were caused by the cleavage and degradation of DNA between nucleosomes. There were no significant changes in telomerase activity of apoptotic cells, while the telomere length shortened markedly. In the meanwhile, cells became more susceptible to apoptotic stimuli and telomere became more vulnerable to degradation after telomerase activity was inhibited. All the results suggest that the apoptosis induced by SB is closely related to telomere shortening, while telomerase enhances resistance of HeLa cells to apoptotic stimuli by protecting telomere. | Jianguo Ren Huili Xia Yaoren Dai | 2001 | Chinese Science Bulletin2001，46，2： | 0 |
| 6 | Expression of Bcl-2 in cells with different telomerase activities显示文摘Both tclomerase and Bcl-2 are important genes in controlling apoptosis. The activation of telomerase and the abnormal regulation of Bcl-2 are also closely related to carcinogenesis. However, little is known about the linkage between telomerase and Bcl-2. The effect of activated telomerase on the expression of Bcl-2 has been investigated. It is demonstrated that in tumor and transformed cells with higher telomerase activity, Bcl-2 expression is significantly lower than that in telomerase negative or less telomerose activity cells. Further study showed that in the telomerase gene-transformed 2BS-fibroblasts, Bcl-2 expression is inhibited significantly while the exogenous telomerase catalyticsubunit gene is re-expressed in flbroblasts. Results indicated that there might be a certain linkage between the expression of telomerase and Bcl-2, and overexpression of exogenous telomerase gene might down regulate the expression of Bcl-2. | CHEN Jing REN Jianguo ZHENG Jianyu CAI Guoping DAI Yaoren | 2001 | Chinese Science Bulletin2001，46，23： | 0 |
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