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| 1 | Application of Next-generation Sequencing Technology in Forensic Science显示文摘Next-generation sequencing(NGS) technology, with its high-throughput capacity and low cost, has developed rapidly in recent years and become an important analytical tool for many genomics researchers. New opportunities in the research domain of the forensic studies emerge by harnessing the power of NGS technology, which can be applied to simultaneously analyzing multiple loci of forensic interest in different genetic contexts, such as autosomes, mitochondrial and sex chromosomes. Furthermore, NGS technology can also have potential applications in many other aspects of research. These include DNA database construction, ancestry and phenotypic inference,monozygotic twin studies, body fluid and species identification, and forensic animal, plant and microbiological analyses. Here we review the application of NGS technology in the field of forensic science with the aim of providing a reference for future forensics studies and practice. | Yaran Yang Bingbing Xie Jiangwei Yan | 2014 | Genomics, Proteomics & Bioinformatics2014，12，5： | 13 |
| 2 | Characterization of miRNomes in Acute and Chronic Myeloid Leukemia Cell Lines显示文摘Myeloid leukemias are highly diverse diseases and have been shown to be associated with microRNA(miRNA) expression aberrations. The present study involved an in-depth miRNome analysis of two human acute myeloid leukemia(AML) cell lines, HL-60 and THP-1, and one human chronic myeloid leukemia(CML) cell line, K562, via massively parallel signature sequencing. mRNA expression profiles of these cell lines that were established previously in our lab facilitated an integrative analysis of miRNA and mRNA expression patterns. miRNA expression profiling followed by differential expression analysis and target prediction suggested numerous miRNA signatures in AML and CML cell lines. Some miRNAs may act as either tumor suppressors or oncomiRs in AML and CML by targeting key genes in AML and CML pathways. Expression patterns of cell type-specific miRNAs could partially reflect the characteristics of K562, HL-60 and THP-1 cell lines, such as actin filament-based processes, responsiveness to stimulus and phagocytic activity. miRNAs may also regulate myeloid differentiation, since they usually suppress differentiation regulators. Our study provides a resource to further investigate the employment of miRNAs in human leukemia subtyping, leukemogenesis and myeloid development. In addition, the distinctive miRNA signatures may be potential candidates for the clinical diagnosis, prognosis and treatment of myeloid leukemias. | Qian Xiong Yadong Yang Hai Wang Jie Li Shaobin Wang Yanming Li Yaran Yang Kan Cai Xiuyan Ruan Jiangwei Yan Songnian Hu Xiangdong Fang | 2014 | Genomics, Proteomics & Bioinformatics2014，12，2： | 6 |
| 3 | DNA methylation and alternative splicing modulate FBXW11 gene expression in Holstein bull testis and are correlated with sperm quality显示文摘F-box and WD-40 domain protein 11(FBXW11)is an important component of the E3 ubiquitin-ligase enzyme that plays a key role in the ubiquitin-dependent regulation of spermatogenesis.In our previous research,the mRNA expression of FBXW11 in bull sperm with high motility is significantly higher than that with low motility.In the present study,the protein expression levels of FBXW11 in bull testicular tissues with low-performance sperm quality groups were significantly higher than those in normal performance groups.The immunohistochemistry result demonstrated that FBXW11 protein was located in the periphery of Leydig cells and seminiferous tubules.Three splice variants of the FBXW11 gene,namely,FBXW11-tv1,FBXW11-tv2,and FBXW11-tv3,were identified in testicular tissues.The splicing patterns of the three variants are exon skipping.The transcript FBXW11-tv2 expressions were the highest in each sample.The low-performance groups displayed higher FBXW11-tv1 and FBXW11-tv2 transcript expressions than the normal performance groups.Two CpG islands were located within the 5’UTR and exon 1-2 region of the FBXW11 gene.Bisulfite sequencing PCR results demonstrated that the methylation levels of 11 methylation sites in the CpG island 2 from−99 to−43 in the normal performance groups were significantly lower than those in the low-performance groups.Pearson correlation analysis suggested that the CpG island 2 methylation level was negatively correlated with sperm motility and the transcript FBXW11-tv2 expression level.Our data revealed that alternative splicing and DNA methylation jointly regulated FBXW11 gene expression and were correlated with sperm quality traits during spermatogenesis in Holsteins. | YONG LIU ZHIHUA JU QIANG JIANG WENHAO LIU CHUNHONG YANG YARAN ZHANG XIUGE WANG YAPING GAO XIAOCHAO WEI YAN SUN JINPENG WANG MINGHAI HOU LING YANG JINMING HUANG | 2021 | BIOCELL2021，45，1： | 1 |
| 4 | Research on the logic monitoring method for the cloud computing based safety computer显示文摘With the development of railway construction in China,the computing demand of the train control system is increasing day by day.The application of cloud computing technology on the rail transit signal system has become a research hotspot in recent years.How to improve the safety and availability of the safety computer platform in the cloud computing environment is the key problem when applying cloud computing to the train operation control system.Since the cloud platform is in an open network environment,fac-ing many security vulnerabilities and malicious network attacks,it is necessary to monitor the operation of computer programmes through edge safety nodes.Firstly,this paper encrypts the logical monitoring method,and then proposes a secure computer de fence model based on the dynamic heterogeneous redundancy structure.Then the continuous time Markov chain(CTMC)is used to quantitatively solve the stable probability of the system,and the influence of different logical monitoring methods on the anti-attack performance of the system is analysed.Finally,the experiment proves that the dynamic heterogeneous redundancy structure composed of encryption logic monitoring can guarantee the safe and stable operation of the safety computer more effectively. | Yaran Yang Lianchuan Ma Tao Tang Huan Ding Zhen Liu | 2023 | Transportation Safety and Environment2023，5，2： | 1 |
| 5 | Design and fabrication of r-hirudin loaded dissolving microneedle patch for minimally invasive and long-term treatment of thromboembolic disease显示文摘Cardiovascular disease is the leading cause of global mortality,with anticoagulant therapy being the main prevention and treatment strategy.Recombinant hirudin(r-hirudin)is a direct thrombin inhibitor that can potentially prevent thrombosis via subcutaneous(SC)and intravenous(IV)administration,but there is a risk of haemorrhage via SC and IV.Thus,microneedle(MN)provides painless and sanitary alternatives to syringes and oral administration.However,the current technological process for the micro mould is complicated and expensive.The micro mould obtained via three-dimensional(3D)printing is expected to save time and cost,as well as provide a diverse range of MNs.Therefore,we explored a method for MNs array model production based on 3D printing and translate it to micro mould that can be used for fabrication of dissolving MNs patch.The results show that r-hirudin-loaded and hyaluronic acid(HA)-based MNs can achieve transdermal drug delivery and exhibit significant potential in the prevention of thromboembolic disease without bleeding in animal models.These results indicate that based on 3D printing technology,MNs combined with r-hirudin are expected to achieve diverse customizableMNs and thus realize personalized transdermal anticoagulant delivery for minimally invasive and long-term treatment of thrombotic disease. | Mengfang Wu Tian Xia Yaran Li Tianfa Wang Shijia Yang Jinchao Yu Qiaoyan Liang Teng Shen Min Yu Bing Zhao | 2022 | Asian Journal of Pharmaceutical Sciences2022，17，2： | 1 |
| 6 | Research on Virtual Coupled Train Control Method Based on GPC&VAPF显示文摘Improving transportation efficiency is an eternal research hotspot in rail transit system.In recent years,the train operation control method based on virtual coupling has attracted the attention of many scholars.The method of train coordination and anti-collision control is not only the key to realize the virtual coupling of train,but also the key to ensure the safety of train operation.Therefore,based on the existing research,a virtual coupled train dynamics model with nonlinear dynamics is established.Then,the parameters of the operation process model of the nonlinear virtual coupled train are identified by the recursive least squares method based on realtime data,which is applied to the variable parameter artificial potential field(VAPF)for parameter identification.A fusion controller based on feature-based generalized model prediction(GPC)and VAPF is used to control the virtual coupled train and prevent collision.Finally,the validity of the proposed method is verified by using real high-speed railway data. | CAO Yuan YANG Yaran MA Lianchuan WEN Jiakun | 2022 | Chinese Journal of Electronics2022，31，5： | 0 |
| 7 | Small-signal modelling and stability analysis of grid-following and grid-forming inverters dominated power system显示文摘In this paper,the explicit state-space model for a multi-inverter system including grid-following inverter-based generators(IBGs)and grid-forming IBGs is developed by the two-level component connection method(CCM),which modularized inverter control blocks at the primary level and IBGs at the secondary level.Based on the comprehensive state-space model representing full order of system dynamics,eigenvalues of the overall system are thoroughly analyzed,identifying potential adverse impacts of not only grid-following inverters,but also grid forming inverters on the system small-signal stability,with the underlying principle of oscillations also understood.Numerical and simulation results validate effectiveness of the proposed methodology on IEEE benchmarking 39-bus system. | Yaran Li Long Fu Qiang Li Wei Wang Yubin Jia Zhao Yang Dong | 2023 | Global Energy Interconnection2023，6，3： | 0 |
| 8 | Development of the Mars energetic particles Completed by IMP analyzer显示文摘The Mars energetic particles analyzer(Mars-EPA for short)is one of the payloads of Mars probe,which de-veloped by IMP and Lanzhou Institute of Physics,will be lunched this year in China. | Zhao Hongyun Qian Yi Yang Haibo Su Hong Kong Jie Sun Zhiyu Yu Yuhong Sun Zhipeng Tang Shuwen Zhang Yongjie Fang Fang She Qianshun Pu Tianlei Yan Junwei Jing Yaran | 2019 | IMP & HIRFL Annual Report2019，，1： | 0 |
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