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| 1 | VvWRKY5 enhances white rot resistance in grape by promoting the jasmonic acid pathway显示文摘Grape white rot is a disease caused by Coniella diplodiella(Speg.)Sacc.(Cd)can drastically reduce the production and quality of grape(Vitis vinifera).WRKY transcription factors play a vital role in the regulation of plant resistance to pathogens,but their functions in grape white rot need to be further explored.Here,we found that the expression of the WRKY IIe subfamily member VvWRKY5 was highly induced by Cd infection and jasmonic acid(JA)treatment.Transient injection and stable overexpression(in grape calli and Arabidopsis)demonstrated that VvWRKY5 positively regulated grape resistance to white rot.We also determined that VvWRKY5 regulated the JA response by directly binding to the promoters of VvJAZ2(a JA signaling suppressor)and VvMYC2(a JA signaling activator),thereby inhibiting and activating the transcription of VvJAZ2 and VvMYC2,respectively.Furthermore,the interaction between VvJAZ2 and VvWRKY5 enhanced the suppression and promotion of VvJAZ2 and VvMYC2 activities by VvWRKY5,respectively.When VvWRKY5 was overexpressed in grape,JA content was also increased.Overall,our results suggested that VvWRKY5 played a key role in regulating JA biosynthesis and signal transduction as well as enhancing white rot resistance in grape.Our results also provide theoretical guidance for the development of elite grape cultivars with enhanced pathogen resistance. | Zhen Zhang Changyue Jiang Cui Chen Kai Su Hong Lin Yuhui Zhao Yinshan Guo | 2023 | Horticulture Research2023，10，10： | 0 |
| 2 | Berry texture QTL and candidate gene analysis in grape(Vitis vinifera L.)显示文摘Berry texture is a noteworthy economic trait for grape;however,the genetic bases and the complex gene expression and regulatory mechanism for the diverse changes in berry texture are still poorly understood.In this study,the results suggest that it is difficult to obtain high-mesocarp firmness(MesF)and high-pericarp puncture hardness(PPH)grape cultivars with high pericarp brittleness(PerB).The high-density linkage map was constructed using whole-genome resequencing based on 151 F1 individuals originating from intraspecific hybridization between the firm-flesh cultivar‘Red Globe’and soft-flesh cultivar‘Muscat Hamburg’.The total length of the consensus map was 1613.17 cM,with a mean genetic distance between adjacent bin markers of 0.59 cM.Twenty-seven quantitative trait loci(QTLs)for berry MesF,PPH,and PerB were identified in linkage groups(LGs)1,3,4,6,8,9,10,11,14,16,and 17,including twelve QTLs that were firstly detected in LGs 6,11,and 14.Fourteen promising candidate genes were identified from the stable QTL regions in LGs 10,11,14,and 17.In particular,VvWARK2 and VvWARK8 refer to chromosome 17 and are two promising candidate genes for MesF and PPH,as the VvWARK8 gene may increase pectin residue binding with WARK for high berry firmness maintenance and the allele for VvWARK2 carrying the‘CC’and‘GA’genotypes at Chr17:1836764 and Chr17:1836770 may be associated with non-hard texture grape cultivars.In addition,real-time quantitative polymerase chain reaction(RT-qPCR)verification revealed that the promising candidate transcription factor genes VvMYB4-like,VvERF113,VvWRKY31,VvWRKY1,and VvNAC83 may regulate cell wall metabolism candidate gene expression for grape berry texture changes. | Hong Lin Li Ma Qiuyu Guo Cheng Liu Yangming Hou Zhendong Liu Yuhui Zhao Changyue Jiang Xiuwu Guo Yinshan Guo | 2023 | Horticulture Research2023，10，12： | 0 |
| 3 | A zwitterionic polymer-inspired material mediated efficient CRISPR-Cas9 gene editing显示文摘The typeⅡ prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR/Cas9) adaptive immune system is a cutting-edge genome-editing toolbox.However,its applications are still limited by its inefficient transduction.Herein,we present a novel gene vector,the zwitterionic polymer-inspired material with branched structure (ZEBRA) for efficient CRISPR/Cas9 delivery.Polo-like kinase 1 (PLK1) acts as a master regulator of mitosis and overexpresses in multiple tumor cells.The Cas9 and single guide sgRNA (sgRNA)-encoded plasmid was transduced to knockout Plk1 gene,which was expected to inhibit the expression of PLK1.Our studies demonstrated that ZEBRA enabled to transduce the CRISPR/Cas9 system with large size into the cells efficiently.The transduction with ZEBRA was cell line dependent,which showed~10-fold higher in CD44-positive cancer cell lines compared with CD44-negative ones.Furthermore,ZEBRA induced highlevel expression of Cas9 proteins by the delivery of CRISPR/Cas9 and efficient gene editing of Plk1 gene,and inhibited the tumor cell growth significantly.This zwitterionic polymerinspired material is an effective and targeted gene delivery vector and further studies are required to explore its potential in gene delivery applications. | Lingmin Zhang Langyu Yang Jionghua Huang Sheng Chen Chuangjia Huang Yinshan Lin Ao Shen ZhouYikang Zheng Wenfu Zheng Shunqing Tang | 2022 | Asian Journal of Pharmaceutical Sciences2022，17，5： | 0 |
| 4 | Morphological diversity of cultured cold-active lytic bacteriophages isolated from the Napahai plateau wetland in China显示文摘Dear Editor,Viruses are the most abundant,diverse,and ubiquitous entities(approximately 1031)on Earth.They play major roles in horizontal gene transfer,the regulation of bacterial community structures,as well as nutrient and energy cycles of marine ecosystems(Danovaro et al.,2008).In particular,lytic bacteriophages(phages)can infect and kill bacteria without harming human or | Xiuling Ji Chunjing Zhang Anxiu Kuang Jiankai Li Yinshan Cui Kunhao Qin Lianbing Lin Benxu Cheng Qi Zhang Yunlin Wei | 2015 | Virologica Sinica2015，30，6： | 0 |
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