| 1 | Discovery of mitochondrial chimeric-gene associated with cytoplasmic male sterility of HL-rice显示文摘The mitochondrial genome libraries of HL-type sterile line(A) and maintainer line(B) have been constructed. Mitochondrial gene, atp6, was used to screen libraries, due to the different Southern and Northern blot results between sterile and maintainer line. Sequencing analysis of positive clones proved that there were two copies of atp6 gene in sterile line and only one in maintainer line. One copy of atpt6 in sterile line was same to that in maintainer line; the other showed different flanking sequence from the 49th nucleotide downstream of the termination codon of atp6 gene. A new chimeric gene, orfH79, was found in the region. OrfH79 had homology to mitochondrial gene cox Ⅱ and orf107, and was special to HL-sterile cytoplasm. | Yl Ping WANG Li SUN Qingping ZHU Yingguo | 2002 | Chinese Science Bulletin2002，47，9： | 36 |
| 2 | Impact of Low Frequency Electro-acupuncture on Glucose and Lipid Metabolism in Unmarried PCOS Women:A Randomized Controlled Trial显示文摘Objective:To explore the effect of electro-acupuncture(EA)on glucose and lipid metabolism in unmarried patients with polycystic ovary syndrome(PCOS).Methods:Fifty-four PCOS patients were equally randomized into true acupuncture group and sham acupuncture group(control)for totally 16 weeks of treatment by random method with a computerized randomization program.Patients in true acupuncture group accepted traditional acupuncture methods with EA and two sets of acupoint groups were used alternatively.The first set consisted of Zhongji(CV 3),Qihai(CV 6),Guilai(ST 29),Sanyinjiao(SP 6),Yinlingquan(SP 9),Hegu(LI4)and Baihui(GV 20),and the second set consisted of Tianshu(ST 25),ST 29,CV 3,CV 6,SP 6,Taichong(LR 3),Neiguan(PC)6 and GV 20.Patients in the sham acupuncture group accepted shallow acupuncture methods through EA without electricity at 4 non-meridian points in each shoulder and upper arm.Outcome measures included body mass index(BMI),waist-hip-ratio(WHR),oral glucose tolerance test(OGTT),insulin release test,glucose and lipid metabolism indicators such as total cholesterol(TC),triglycerides,high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol,adiponectin,leptin,visfatin,resistin,and interleukin(IL-6).Results:Twenty-six subjects in the true acupuncture group and 20 subjects in the sham group completed the clinical trial.After 16 weeks of treatment,no significant difference in the outcome measures were observed between the two groups(P>0.05).However,as compared with baseline data,a reduction in weight,BMI,hipline,WHR,fasting glucose,homeostatic model assessment of insulin sensitivity,visfatin and HDL-C,and an increase in resistin and IL-6 were observed in the true acupuncture group(P<0.05).In addition,a reduction in visfatin and an increase in TC were also observed in the sham group(P<0.05).Conclusions:Acupuncture may have a beneficial effect in the treatment of PCOS by improving glucose and lipid metabolism.Moreover,the sham acupuncture may be not completely ineffective.Sham acupuncture may improve some of the aspects of the glucose and lipid metabolism of PCOS patients through a placebo effect.(Registration Nos.ChiCTR-TRC-12002529 and NCT01812161) | DONG Hao-xu WANG Qing WANG Zhi WU Xiao-ke CHENG Ling ZHOU Zhong-ming YANG Li Yl Ping HUANG Dong-mei | 2021 | Chinese Journal of Integrative Medicine2021，27，10： | 4 |
| 3 | The editing sites in transcripts of functional genes of rice mitochondria显示文摘RNA editing exists extensively in the higher plant mitochondria, and is a required step for forming functional proteins. There may be some relationship between RNA editing and cytoplasmic male sterility (CMS), a kind of phenomenon that is attributed to mitochondrial genome mutations. The research materials used are the gametophytic male sterility line (A), maintainer line (B) and F1 hybrid (F1) of HL-type CMS rice. cDNAs and DNAs of atp6 and coxII have been obtained from A, B and F1 by PCR and RT-PCR. Comparing sequences of cDNAs and DNAs, 18 and 15 editing sites were found respectively in the transcripts of atp6 and coxll. A, B and F1 shared the same editing sites. RNA editing improves hydrophobicity and conservation of the predicted protein as compared with other organisms. | Yl Ping WANG Li SUN Qingping ZHU Yingguo | 2002 | Chinese Science Bulletin2002，47，11： | 3 |
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