2篇
您的检索式：作者名="YuLongjiang"
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| 1 | Micro-propagation of Lepedium meyenii Walp. (Maca) by shoot culture显示文摘The callus induction and plant regeneration system for an important plant, Lepedium meyenii Walp., has been established. Calli were induced from cotyledons petioles of Lepedium meyenii Walp within 4 weeks in a modified MS medium supplemented with BA plus NAA. The highest percentage of callus formation (57. 1% ) was found on MS medium supplemented with 0. 5 mg l^-1 BA and 0. 5 mg l^-1 NAA. During subculture on the shoot formation medium, most of calli proliferated and 50% -60% formed shoots. About 66.7% of shoots formed into roots on 1/2 strength MS containing 0.5mg l^-1 IBA after 4 weeks in culture. Chromosome count confirmed the number of the regeneration Maca plantlet was the same as that of the native plant(8x=64) .For regeneration of plantlets, from seedling via primary callus production, a four-step process of organogenes is required about 16 weeks. | ChengHua YuLongjiang HuQiongyue SunYouping JinWenwen ChenShancai LiMaoteng | 2004 | Agricultural Science & Technology2004，5，4： | 2 |
| 2 | Medium optimization to improve astaxanthin production of Xanthophyllomyces dendrorhous mutant W6-8 based on genetic algorithms显示文摘Genetic algorithms (GA) based on the principle of mimicing Darwinian evolution and survival of the fittest in a natural environment was used to optimize the medium for astaxanthin production by the mutant strain W6-8 of Xantho-phyllomyces dendrorhous. The 50 concentration levels of 6 medium components were optimized within 50 experiments (full experimental plan: 50^6 experiments). The results showed that GA could be applied in the medium optimization and better results were obtained. By employing optimized medium components (glucose 39.8 g l^-1, yeast extract 4.08 g l^-1,(NH4)2SO4 7.36 g l^-1, MgSO4 2 g l^-1, K2HPO4 2.04 g l^-1 and KH2PO4 3.48 g l^-1), the highest astaxanthin production was 9.855 mg l^-1, approximately 31% higher than that under the initial conditions, and was approximately 15.46% higher than that by orthogonal array but only slightly higher than that by response surface methodology. In the sequent scale-up experiments, the astaxanthin yield was obtained approximately 14.753 mg l^-1, employing the optimal medium. The results indicated that GA, as an euiicient method for medium optimization, was superior to other optimal means such as orthogonal array. | WangWenjun YuLongjiang HePu ZhouPengpeng | 2004 | Agricultural Science & Technology2004，5，4： | 0 |
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