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| 1 | Protein farnesylation negatively regulatesbrassinosteroid signaling via reducing BES1 stabilityinArabidopsis thaliana^(∞)显示文摘Brassinosteroids(BRs)are a group of steroidal phytohormones,playing critical roles in almost all physiological aspects during the life span of a plant.In Arabidopsis,BRs are perceived at the cell surface,triggering a reversible phosphorylation-based signaling cascade that leads to the activation and nuclear accumulation of a family of transcription factors,represented by BES1 and BZR1.Protein famesylation is a type of post-translational modification,functioning in many important cellular processes.Previous studies demonstrated a role of famesylation in BR biosynthesis via regulating the endoplasmic reticulum localization of a key bassino-lide(BL)biosynthetic enzyme BR6ox2.Whether such a process is also involved in BR signaling is not understood.Here,we demonstrate that protein famesylation is involved in mediating BR signaling in Arabidopsis.A loss-of-function mutant of EN HANCED RESPONSE TO ABA 1(iERA1).encoding a(3 subunit of the protein famesyl transferase hoi oenzyme,can alter the BL sensitivity of bak1~4 from a reduced to a hypersensitive level,era 7 can partially rescue the BR defective phenotype of a hetero zygous mutant of bin2-1,a gain-of function mutant of BIN2 which encodes a negative regulator in the BR signaling.Our genetic and biochemical analyses revealed that ERA1 plays a significant role in regulating the protein stability of BES1. | Zengxiu Feng Hongyong Shi Minghui Lv Yuang Ma Jia Li | 2021 | Journal of Integrative Plant Biology2021，63，7： | 3 |
| 2 | Taurine and glycine activate the same CI- conductance in substancia nigra dopamine neurons 显示文摘 | Yuang WH Lacey MG | 1992 | Brain Res1992，571，： | 1 |
| 3 | Evolution of chemistry and selection technology for DNA-encoded library显示文摘DNA-encoded chemical library(DEL)links the power of amplifiable genetics and the nonself-replicating chemical phenotypes,generating a diverse chemical world.In analogy with the biological world,the DEL world can evolve by using a chemical central dogma,wherein DNA replicates using the PCR reactions to amplify the genetic codes,DNA sequencing transcripts the genetic information,and DNA-compatible synthesis translates into chemical phenotypes.Importantly,DNA-compatible synthesis is the key to expanding the DEL chemical space.Besides,the evolution-driven selection system pushes the chemicals to evolve under the selective pressure,i.e.,desired selection strategies.In this perspective,we summarized recent advances in expanding DEL synthetic toolbox and panning strategies,which will shed light on the drug discovery harnessing in vitro evolution of chemicals via DEL. | Peixiang Ma Shuning Zhang Qianping Huang Yuang Gu Zhi Zhou Wei Hou Wei Yi Hongtao Xu | 2024 | Acta Pharmaceutica Sinica B2024，14，2： | 0 |
| 4 | Research on the Impacts of the Inertia and Droop Control Gains from a Variable-Speed Wind Turbine Generator on the Frequency Response显示文摘System frequency must be kept very close to its nominal range to ensure the stability of an electric power grid.Excessive system frequency variations are able to result in load shedding,frequency instability,and even generator damage.With increasing wind power penetration,there is rising concern about the reduction in inertia response and primary frequency control in the electric power grid.Converter-based wind generation is capable of providing inertia response and primary frequency response;nevertheless,the primary frequency and inertia responses of wind generation are different from those of conventional synchronous fleets;it is not completely understood how the primary frequency and inertia responses affect the given system under various disturbances and available kinetic energy levels.Simulations are used to investigate the influences of inertia and droop control strategies on the dynamic frequency responses,particularly the index of the second frequency drop under various disturbance and wind conditions.A quantitative analysis provides insight into setting of inertia and droop control coefficients for various wind and disturbance conditions to facilitate adequate dynamic frequency responses during frequency events. | Dejian Yang Yien Xu Tong Zhu Yang Wang Qiuhan Cao Yuang Ma Enshu Jin Xinsong Zhang Haochen Sun | 2022 | Energy Engineering2022，119，2： | 0 |
| 5 | Cloning,expression and function of the extracellular fragment of human TRAIL gene显示文摘To obtain the recombinant soluble protein of the extracellular fragment of human TRAIL gene and to identify its function preliminarily,this gene fragment was amplified from peripheral blood mononu- clear cells(PBMC)by RT-PCR and cloned into vector pGEM-T-Easy for sequence analysis.The expres- sion vector pET-30a/TRAIL was then constructed by DNA recombination method with a His-tag gene at the front of the TRAIL fragment,and the recombinant protein was expressed in E.coli BL21(DE3). Meanwhile,the expressed target protein was purified with Ni-NTA chromatography column and identified by SDS-PAGE and Western blotting.The proliferation inhibition activity of TRAIL-His was detected by MTT assay.PI staining and Wright-Giemsa staining were used to detect the presence of the TRAIL-in- duced cell apoptosis.It was demonstrated that the target protein expressed in E.coli BL21 showed the same relative molecular mass as that the protein expected and could be recognized by both the anti-TRAIL polyclonal antibody and anti-His monoclonal antibody.In addition,this protein could also inhibit prolif- eration of human lymphoma cell line Jurkat cells or induce apoptosis of this cell line.It is apparent that a recombinant soluble TRAIL protein with biological activity is obtained and this prospective study can lay solid foundation for further research on the biological activity and application in the anti-tumor therapy. | Yu GANG WANG KUN PENG ZHAO Ju GAO CHEN YAN LI YUANG FANG MA BEI FEN SHEN | 2007 | Journal of Microbiology and Immunology2007，5，2： | 0 |
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