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| 1 | A new mechanism of invader success: Exotic plant inhibits natural vegetation restoration by changing soil microbe com-munity显示文摘Since the 1950s of the last century, the exotic plant, Eupatorium adenophorum, has spread rapidly across southwest China, damaging native ecosystems and causing great economic losses. We examined the pH, N, P, K, and organic matter concentrations, and the bacterial community character (by Biolog EcoPlateTM) in soils from sites heavily and lightly invaded by this exotic species. Also, soil from the lightly invaded site was treated with a water extract of E. adenophorum roots to examine the effect of the plant on soil properties. We grew three plant species, one native and two exotic, in pot experiment using soil from heavily invaded site to examine the effects of the soil on these plants growth. The soil analysis demonstrated that the pH, organic matter, total N, total P and total K in soils from the heavily invaded site were only slightly different from those of the lightly invaded site, but concentrations of NH4+, NO?3 and available P and K in the heavily invaded site were greater than those in the lightly invaded site. The catabolic activity of soil bacterial community in the heavily invaded site was different from that in the lightly invaded site. The catabolic activity of bac- terial community in soils treated by the water extract of E. adenophorum roots changed and became similar to that in soils from the heavily invaded site. The pot experiment showed that the exotic plants growth in heavily invaded soil were not different from in lightly invaded soil; however, the native plant biomass decreased dramatically when grown in soil from the heavily invaded site as compared to soil from the lightly invaded site; and the same phenomenon was found when any potential allelopathic effects by E. adeno- phorum were eliminated by added activated carbon to those soils. Difference in soil nutrient availability and allelopathy could not explain this phenomenon of the native plant in the soils from the heavily and lightly invaded sites. Changes ob- served in the soil bacterial community were obviously related to native plant growth in those tow soils. Those results sug- gest that changing soil microbial community may be an im- portant part of E. adenophorum invasion process. Since the soil microbial community serves as bridge in connection of exotic and natural plants, the exotic plant could inhibit the natural plant growth and reproduction by changing the soil microbial community in invaded site. | YUXingjun YUDan LUZhijun MAKeping | 2005 | Chinese Science Bulletin2005，50，11： | 41 |
| 2 | Quercetin induces apoptosis by activating caspase-3 and regulating Bcl-2 and cyclooxygenase-2 pathways in human HL-60 cells显示文摘橡黄素是自然地发生之一饮食的黄酮醇混合物。它在植物富有地是在场的并且有 chemopreventive 和 anticancer 效果。调查它的 anticancer 机制,我们对尖锐白血病房间线检验了橡黄素的活动, HL-60。我们的结果证明橡黄素禁止了房间增长并且在一个时间依赖者和剂量依赖者举止导致了 apoptosis。而且,橡黄素下面调整 anti-apoptosis 蛋白质 Bcl-2 的表示并且起来调整 pro-apoptosis 蛋白质 Bax 的表示。Caspase-3 被橡黄素也激活,它开始了一条 caspase-3-depended mitochodrial 小径导致 apoptosis。橡黄素禁止了 cycloocygenase-2 (Cox-2 ) 的表示,这也被发现 mRNA 和 Cox-2 蛋白质。一起拿,这些调查结果建议橡黄素由禁止 Cox-2 表示在一条 caspase-3-dependent 小径导致 apoptosis 并且调整下游的 apoptotic 部件的表示,包括 Bcl-2 和 Bax。橡黄素能是可能安全地在白血病治疗被使用的一个有势力和有希望的药。 | Guomin Niu Songmei Yin Shuangfeng Xie Yiqing Li Danian Nie Liping Ma Xiuju Wang Yudan Wu | 2011 | Acta Biochimica et Biophysica Sinica2011，43，1： | 28 |
| 3 | Endogenous neurogenesis in adult mammals after spinal cord injury显示文摘During the whole life cycle of mammals,new neurons are constantly regenerated in the subgranular zone of the dentate gyrus and in the subventricular zone of the lateral ventricles.Thanks to emerging methodologies,great progress has been made in the characterization of spinal cord endogenous neural stem cells(ependymal cells) and identification of their role in adult spinal cord development.As recently evidenced,both the intrinsic and extrinsic molecular mechanisms of ependymal cells control the sequential steps of the adult spinal cord neurogenesis.This review introduces the concept of adult endogenous neurogenesis,the reaction of ependymal cells after adult spinal cord injury(SCI),the heterogeneity and markers of ependymal cells,the factors that regulate ependymal cells,and the niches that impact the activation or differentiation of ependymal cells. | Hongmei Duan Wei Song Wen Zhao Yudan Gao Zhaoyang Yang Xiaoguang Li | 2016 | Science China(Life Sciences)2016，59，12： | 10 |
| 4 | Mesomechanism of steel fiber reinforcement and toughening of reactive powder concrete显示文摘Reactive powder concrete (RPC) is a novel cement-based composite material with ultra-high strength. Embedding a certain amount of short steel fibers in the matrix can improve the RPC’s toughness and overcome the disadvantage of high brittle- ness. In this paper, a number of direct uniaxial tension tests have been carried out with ‘8-shape’ RPC200 specimens. The bond-slip process, mesoscopic structural variation and mechanical characteristics of a fiber pullout of the matrix have been investigated using the real-time SEM loading system and CCD observation tech- niques. The influence of the volume of embedded short steel fibers in matrix on the mesoscopic morphology of attachments on the surface of a pulled individual fiber, the initial cracking force, the ultimate pullout force, interfacial bond strength and the pullout rupture energy have been analyzed. A general formulation relating these quantities to the volume of fibers in matrix has been proposed. The components comprising the interfacial bond strength have been outlined. In addition, the con- tribution that fibers make to enhance and toughen the reactive powder concrete has been discussed. It is shown that there exists an optimal threshold of fiber volume ρv, opt =1.5% at which the bond performance of a fiber pullout of RPC be- haves best. | JU Yang JIA YuDan LIU HongBin CHEN Jian | 2007 | Science China(Technological Sciences)2007，50，6： | 9 |
| 5 | Toughness and characterization of reactive powder concrete with ultra-high strength显示文摘A number of three-point bending and fracture tests of 200 MPa-level reactive powder concrete (RPC) with the various fiber contents have been conducted to probe the nature and characteristics of tough- ness of RPC200. The contribution of the embedded fibers to improving the crack-resistant capacity, energy absorption capacity and toughness with various deformation mechanisms has been analyzed. Taking account of that the first-crack deformation, peak-load deformation and their improvement varied with the fiber contents and that the deformation mechanism affected differently the performance at the first crack and the peak load, we took the peak-load deformation of plain RPC200 as the reference de- formation to measure the toughness of fibered RPC200. Two toughness indices T2(n?1)(n) and FT2(n?1)(n) have been formulated based on P-δ responses and P-CMOD responses. The indices quantify the tough- ness of RPC200 with the various deformation mechanisms relative to perfectly elastoplastic materials by setting the toughness level 2(n?1) as the initial reference. It is shown that the toughness index T2(n?1)(n) reflects the function of fibers to improve the toughness of RPC with the deformation throughout specimens, but overestimates the contribution to enhancing the toughness in post-peak periods. It underestimates, on other hands, the contribution to improving the toughness in the period from the first crack to the peak load. In contrast, the toughness index FT2(n?1)(n) properly presents the capability that fibers absorb energy and constrain crack propagation in the matrix when the deformation is con- centrated on the open crack. The proposed index unveils the contribution of fibers to toughening RPC200 both in the period from the first-crack to the peak load and in the period of post peak. This characterization method not only reveals the nature of toughness but also levels the toughness of RPC200. It could provide a way to establish an objective toughness characterization for RPC200 and facilitate its applications. | Yang Ju HongBin Liu Jian Chen YuDan Jia PeiHuo Peng | 2009 | Science China(Technological Sciences)2009，52，4： | 7 |
| 6 | SKP2 siRNA inhibits the degradation of P27^kip1 and down-regulates the expression of MRP in HL-60/A cells显示文摘S 阶段联系 kinase 的蛋白质 2 (SKP2 ) 基因是肿瘤 suppressor 基因,并且涉及 P27kip1 的调停 ubiquitin 的降级。SKP2 和 P27kip1 影响通过调整白血病房间的增长, apoptosis 和区别继续和白血病的预后。在这研究,我们探索了通过 SKP2 下面规定 HL-60/A 药抵抗逆行的机制。HL-60/A 房间是由有 SKP2 siRNA 的 Amaxa Nucleofector 系统的 nucleofected。Skp2, P27kip1,和多药抵抗的基因和蛋白质表示层次联系了蛋白质(MRP ) 被反向的抄写聚合酶链反应和西方的污点分析分别地决定。房间周期被流动 cytometry 分析。50% 禁止的集中价值在 chemotherapeutics 的不同集中下面根据这些二种房间的死亡率用细胞毒素的分析被计算比较房间的敏感。HL-60/A 房间显示出由抗力移转特征的多药抵抗显型到 adriamycin, daunorubicin,和 arabinosylcytosine,由于 MRP 的表示。我们发现 SKP2 的表示比在 HL-60 房间在 HL-60/A 房间是更高的,但是 P27kip1 的表示更低。而 P27kip1 的蛋白质水平是起来调整的,在由 SKP2 siRNA 的 HL-60/A 房间 nucleofected 的 SKP2 的表示是下面调整的。与在控制组(由控制 siRNA 的 nucleofected ) 的 MRP 表达式水平相比, mRNA 和蛋白质表达式由 SKP2 siRNA 在 HL-60/A 房间 nucleofected MRP 铺平更低,并且后者房间对 adriamycin, daunorubicin,和 arabinosylcytosine 更敏感。下面调整在 G0/G1 阶段的 SKP2 表示和逮捕房间与下面调整的 MRP 改进白血病房间的药敏感表示。 | Jie Xiao Songmei Yin Yiqing Li Shuangfeng Xie Danian Nie Liping Ma Xiuju Wang Yudan Wu Jianhong Feng | 2009 | Acta Biochimica et Biophysica Sinica2009，41，8： | 7 |
| 7 | Application of the sodium hyaluronate-CNTF scaffolds in repairing adult rat spinal cord injury and facilitating neural network formation显示文摘The present study aimed to explore the potential of the sodium hyaluronate-CNTF (ciliary neurotrophic factor) scaffold in activating endogenous neurogenesis and facilitating neural network re-formation after the adult rat spinal cord injury (SCI). After completely cutting and removing a 5-mm adult rat T8 segment, a sodium hyaluronate-CNTF scaffold was implanted into the lesion area. Dil tracing and immunofluorescence staining were used to observe the proliferation, differentiation and integration of neural stem cells (NSCs) after SCI. A planar multielectrode dish system (MED64) was used to test the electrophysiological characteristics of the regenerated neural network in the lesioned area. Electrophysiology and behavior evaluation were used to evaluate functional recovery of paraplegic rat hindlimbs. The Dil tracing and immunofluorescence results suggest that the sodium hyaluronate-CNTF scaffold could activate the NSCs originating from the spinal cord ependymal, and facilitate their migration to the lesion area and differentiation into mature neurons, which were capable of forming synaptic contact and receiving glutamatergic excitatory synaptic input. The MED64 results suggest that functional synapsis could be established among regenerated neurons as well as between regenerated neurons and the host tissue, which has been evidenced to be glutamatergic excitatory synapsis. The electrophysiology and behavior evaluation results indicate that the paraplegic rats’ sensory and motor functions were recovered in some degree. Collectively, this study may shed light on paraplegia treatment in clinics. | Yabin Xie Wei Song Wen Zhao Yudan Gao Junkui Shang Peng Hao Zhaoyang Yang Hongmei Duan Xiaoguang Li | 2018 | Science China(Life Sciences)2018，61，5： | 6 |
| 8 | Sandbox Modeling of the Fault-increment Pattern in Extensional Basins显示文摘Three series of sandbox modeling experiments were performed to study the fault-increment pattern in extensional basins. Experimental results showed that the tectonic action mode of boundaries and the shape of major boundary faults control the formation and evolution of faults in extensional basins. In the process of extensional deformation, the increase in the number and length of faults was episodic, and every 'episode' experienced three periods, strain-accumulation period, quick fault-increment period and strain-adjustment period. The more complex the shape of the boundary fault, the higher the strain increment each 'episode' experienced. Different extensional modes resulted in different fault-increment patterns. The horizontal detachment extensional mode has the 'linear' style of fault-increment pattern, while the extensional mode controlled by a listric fault has the 'stepwise' style of fault-increment pattern, and the extensional mode controlled by a ramp-flat boundary fault has the 'stepwise-linear' style of fault-increment pattern. These fault-increment patterns given above could provide a theoretical method of fault interpretation and fracture prediction in extensional basins. | Geng Changbo Tong Hengmao He Yudan Wei Chunguang | 2007 | Petroleum Science2007，4，2： | 6 |
| 9 | Identification and Characterization of a LEA-like Gene, CaMF5,Specifically Expressed in the Anthers of Male-fertile Capsicum annuum显示文摘To improve the understanding of molecular mechanisms of anther and/or pollen development in Chili pepper, in the present study, fulllength cDNA and DNA sequences of the pollen development-related gene CaMF5 were obtained from the anthers of a Capsicum annuum nuclear male-fertile line. Sequence analysis indicated that the full length of CaMF5 was 747 bp, containing a maximum opening reading frame of 447 bp.Amino acid sequence alignment and phylogenetic analysis revealed that CaMF5 shared approximately 37%–77% homology with a series of uncharacterized or hypothetical proteins and late embryogenesis abundant(LEA) proteins from other plants. However, no LEA structural domain was detected in CaMF5, which indicated that it might be a new type of LEA gene. CaMF5 was only expressed in flower buds at stages 7 and 8 and in open flowers of the male-fertile line, whereas it exhibited no expression in any examined organs of the male-sterile line. In addition, CaMF5 showed the highest transcript abundance in the anthers of the male-fertile line, with no expression being detected in any other examined organs, such as the sepals, petals, pistils, roots, stems, or leaves. Taken together, our results suggest that CaMF5 is an anther-specific gene that might encode a new type of LEA protein related to anther and/or pollen development in C. annuum. | Yudan Wang Guoju Chen Jianjun Lei Bihao Cao Changming Chen | 2020 | Horticultural Plant Journal2020，6，1： | 6 |
| 10 | Effective Improvement of the Photovoltaic Performance of Carbon-Based Perovskite Solar Cells by Additional Solvents显示文摘A solvent-assisted methodology has been developed to synthesize CH_3NH_3 PbI_3perovskite absorber layers.It involved the use of a mixed solvent of CH_3NH_3 I,PbI_2,c-butyrolactone,and dimethyl sulfoxide(DMSO) followed by the addition of chlorobenzene(CB).The method produced ultra-flat and dense perovskite capping layers atop mesoporous TiO_2 films,enabling a remarkable improvement in the performance of free hole transport material(HTM) carbon electrode-based perovskite solar cells(PSCs).Toluene(TO) was also studied as an additional solvent for comparison.At the annealing temperature of 100 °C,the fabricated HTM-free PSCs based on drop-casting CB demonstrated power conversion efficiency(PCE) of 9.73 %,which is 36 and 71 % higher than those fabricated from the perovskite films using TO or without adding an extra solvent,respectively.The interaction between the PbI_2–DMSO–CH_3NH_3I intermediate phase and the additional solvent was discussed.Furthermore,the influence of the annealing temperature on the absorber film formation,morphology,and crystalline structure was investigated and correlated with the photovoltaic performance.Highly efficient,simple,and stable HTM-free solar cells with a PCE of 11.44 % were prepared utilizing the optimum perovskite absorbers annealed at 120 °C. | Chenxi Zhang Yudan Luo Xiaohong Chen Yiwei Chen Zhuo Sun Sumei Huang | 2016 | Nano-Micro Letters2016，8，4： | 4 |
| 11 | Evaluation of the four potential Cretaceous-Paleogene (K-Pg) boundaries in the Nanxiong Basin based on evidences from volcanic activity and paleoclimatic evolution显示文摘Determining the location of the Cretaceous-Paleogene(K-Pg) boundary in terrestrial strata is highly significant for studying the evolution of terrestrial ecosystems at the end of the Cretaceous(especially the extinction of non-avian dinosaurs). At present, research on terrestrial K-Pg boundaries worldwide is concentrated in the middle and high latitudes, such as North America and Northeast China. Although many studies have also been carried out in the Nanxiong Basin, located at low latitudes(which has become the standard for dividing and comparing the continental K-Pg stratigraphy in China), many researchers have proposed four possible boundaries from different perspectives. Therefore, the exact location remains to be determined. In this study, the total mercury(Hg) content, environmental magnetism, geochemistry, and other parameters for the samples collected near the four boundaries were determined and compared with existing records. Results indicated that: 1) The total Hg content significantly increased in the upper part of the Zhenshui Formation and Pingling part of the Shanghu Formation with sharp fluctuations. As per latest dating results of Deccan Traps, the significantly high Hg value was attributed to the Deccan Traps eruption. Boundary 1 was located in the middle of the Hg anomaly interval, which was consistent with the relationship between the global K-Pg boundary and time of volcanic eruption. 2) The reconstructed paleoclimate evolution curve revealed that the red sediments in the basin recorded the late Maastrichtian warming event(66.2 Ma). Regarding the relationship between the four boundaries and this warming event, only boundary 1 was found to be closest to the real K-Pg boundary of the Nanxiong Basin. | Mengting ZHAO Mingming MA Mei HE Yudan QIU Xiuming LIU | 2021 | Science China Earth Sciences2021，64，4： | 4 |
| 12 | Flow-resistance analysis of nano-confined fluids inspired from liquid nano-lubrication:A review显示文摘How to reduce flow resistance of nano-confined fluids to achieve a high flux is a new challenge for modern chemical engineering applications, such as membrane separation and nanofluidic devices. Traditional models are inapplicable to explain the significant differences in the flow resistance of different liquid–solid systems.On the other hand, friction reduction in liquid nano-lubrication has received considerable attention during the past decades. Both fields are exposed to a common scientific issue regarding friction reduction during liquid–solid relative motion at nanoscale. A promising approach to control the flow resistance of nano-confined fluids is to reference the factors affecting liquid nano-lubrication. In this review, two concepts of the friction coefficient derived from fluid flow and tribology were discussed to reveal their intrinsic relations. Recent progress on low or ultra-low friction coefficients in liquid nano-lubrication was summarized based on two situations. Finally, a new strategy was introduced to study the friction coefficient based on analyzing the intermolecular interactions through an atomic force microscope(AFM), which is a cutting-point to build a new model to study flowresistance at nanoscale. | Xianzhu Huang Jian Wu Yudan Zhu Yumeng Zhang Xin Feng Xiaohua Lu | 2017 | Chinese Journal of Chemical Engineering2017，25，11： | 3 |
| 13 | Physicochemical properties and structure of fluid at nano-/micro-interface:Progress in simulation and experimental study显示文摘In modern chemical engineering processes, the involvement of solid/fluid interface is the most important component of process intensification techniques, such as confined membrane separation and catalysis. In the review, we summarized the research progress of the latest theoretical and experimental works to elucidate the contribution of interface to the fluid properties and structures at nano-and micro-scale. We mainly focused on water, alcohol aqueous solution, and ionic liquids, because they are classical systems in interfacial science and/or widely involved in the industrialization process. Surface-induced fluids were observed in all reviewed systems and played a critical role in physicochemical properties and structures of outside fluid. It can even be regarded as a new interface, when the adsorption layer has a strong interaction with the solid surface. Finally, we proposed a perspective on scientific challenges in the modern chemical engineering processes and outlined future prospects. | Qingwei Gao Yumeng Zhang Shuting Xu Aatto Laaksonen Yudan Zhu Xiaoyan Ji Xiaohua Lu | 2020 | Green Energy & Environment2020，5，3： | 3 |
| 14 | Progress in molecular-simulation-based research on the effects of interface-induced fluid microstructures on flow resistance显示文摘In modern chemical engineering processes, solid interface involvement is the most important component of process intensification techniques, such as nanoporous membrane separation and heterogeneous catalysis. The fundamental mechanism underlying interfacial transport remains incompletely understood given the complexity of heterogeneous interfacial molecular interactions and the high nonideality of the fluid involved. Thus, understanding the effects of interface-induced fluid microstructures on flow resistance is the first step in further understanding interfacial transport. Molecular simulation has become an indispensable method for the investigation of fluid microstructure and flow resistance. Here, we reviewed the recent research progress of our group and the latest relevant works to elucidate the contribution of interface-induced fluid microstructures to flow resistance.We specifically focused on water, ionic aqueous solutions, and alcohol–water mixtures given the ubiquity of these fluid systems in modern chemical engineering processes. We discussed the effects of the interfaceinduced hydrogen bond networks of water molecules, the ionic hydration of ionic aqueous solutions, and the spatial distributions of alcohol and alcohol–water mixtures on flow resistance on the basis of the distinctive characteristics of different fluid systems. | Yumeng Zhang Yudan Zhu AnranWang Qingwei Gao Yao Qin Yaojia Chen Xiaohua Lu | 2019 | Chinese Journal of Chemical Engineering2019，27，6： | 2 |
| 15 | Effect of HS2 and HS3 elements on erythroid-specific expression in transgenic mice显示文摘The expression plasmids CMV/GFP, HS2ALL, HS3ALL and HS23ALL were selected to investigate the effect of HS2 and HS3 element on erythroid-specific expression in transgenic mice. These plasmids were digested with restriction enzymes and purified. And five DNA fragments, CMV/GFP, HS2/GFP, CMV/HS2/GFP, HS23/GFP and HS3/GFP were obtained. After purification, the above DNA fragments were microinjected into the pre-nuclei of the mice fertilized eggs and transgenic mice were generated, with an integration rate of 10.89%. The green fluorescence protein(GFP) expression in many transgenic mouse tissues was determined by FACS analysis. The results showed that the HS2 and 1.7 kb of β-globin gene promoter were sufficient for the erythroid-specific expression of β-globin gene. The GFP expression of different recombinant constructs was also analyzed in blood of all the transgenic mice with FACS. The results indicated that HS2 and HS3 had the same enhancement activity on the regulation of β-globin gene expression. Moreover, these two elements showed a significant synergistic effect on gene expression at the transgenic mouse level, although the GFP expression varied largely among different transgenic mouse litters. | JIA Chunping, YAN Jingbin, XIAO Yanping, FANG Yudan, HUANG Shuzheng and ZENG Yitao(Institute of Medical Genetics, Shanghai Jiaotong University, Shanghai 200040, China) | 2003 | Progress in Natural Science:Materials International2003，13，9： | 2 |
| 16 | Effects of the Molecular Weight of PLGA on Degradation and Drug Release In vitro from an mPEG-PLGA Nanocarrier显示文摘 | LIU Rui WANG Yan MA Yudan WU Yi GUO Yi XU Li | 2016 | Chemical Research in Chinese Universities2016，32，5： | 2 |
| 17 | Extra low friction coefficient caused by the formation of a solid-like layer:A new lubrication mechanism found through molecular simulation of the lubrication of MoS_2 nanoslits显示文摘Monolayer molybdenum disulfide(MoS2) is a novel two-dimensional material that exhibits potential application in lubrication technology. In this work, molecular dynamics was used to investigate the lubrication behaviour of different polar fluid molecules(i.e., water, methanol and decane) confined in monolayer Mo S2 nanoslits. The pore width effect(i.e., 1.2, 1.6 and 2.0 nm) was also evaluated. Results revealed that decane molecules exhibited good lubricating performance compared to the other two kinds of molecules. The friction coefficient followed the order of decane b methanol b water, and decreased evidently as the slit width increased, except for decane. Analysis of the spatial distribution and mobility of different confined fluid molecules showed that a solid-like layer was formed near the slit wall. This phenomenon led to the extra low friction coefficient of confined decane molecules. | Jiahui Li Yudan Zhu Yumeng Zhang Qingwei Gao Wei Zhu Xiaohua Lu Yijun Shi | 2018 | Chinese Journal of Chemical Engineering2018，26，12： | 2 |
| 18 | A novel scheme for ultrashort terahertz pulse generation over a gapless wide spectral range:Raman-resonance-enhanced four-wave mixing显示文摘Ultrashort energetic terahertz(THz)pulses have created an exciting new area of research on light interactions with matter.For material studies in small laboratories,widely tunable femtosecond THz pulses with peak field strength close to MV cm-1 are desired.Currently,they can be largely acquired by optical rectification and difference frequency generation in crystals without inversion symmetry.We describe in this paper a novel scheme of THz pulse generation with no frequency tuning gap based on Raman-resonance-enhanced four-wave mixing in centrosymmetric media,particularly diamond.We show that we could generate highly stable,few-cycle pulses with near-Gaussian spatial and temporal profiles and carrier frequency tunable from 5 to>2o THz.They had a stable and controllable carrier-envelop phase and carried~15 nJ energy per pulse at 10 THz(with a peak field strength of~1 MV cm^(-1)at focus)from a O.5-mm-thick diamond.The measured THz pulse characteristics agreed well with theoretical predictions.Other merits of the scheme are discussed,including the possibility of improving the THz output energy to a much higher level. | Jiaming Le Yudan Su Chuanshan Tian A.H.Kung Y.Ron Shen | 2023 | Light(Science & Applications)2023，12，2： | 2 |
| 19 | The role of AMP-activated protein kinase in quercetin-induced apoptosis of HL-60 cells显示文摘我们的以前的研究证明了橡黄素禁止 Cox-2 和 Bcl-2 表情,并且导致人的白血病 HL-60 cellapoptosis。以便在 HL-60cells 的导致橡黄素的 apoptosis 上调查激活安培的蛋白质 kinase (AMPK ) 的角色,我们使用了流动 cytometry 检测房间 apoptosis。LKB1, phosphorylated AMPK (p-AMPK ) ,和 Cox-2 proteinwere 的表情由西方的污点在 HL-60 房间和正常的外部血 mononuclear 房间(PBMC ) 检测了。LKB1, p-AMPK,和 Cox-2 的表情与橡黄素在文化以后在 HL-60 房间被检测。p-AMPK 的表情与 AMPK 禁止者混合物 C 在文化以后在 HL-60cells 被检测。然后, LKB1, p-AMPK,和 Cox-2 的表情与独自一个的橡黄素或橡黄素 + 混合物 C 在文化以后在 HL-60cells 被检测。在 PBMC 和 HL-60 之间没有重要 differencein LKB1,这被发现。在 PBMC 的 p-AMPK 在 HL-60 比那高,当 Cox-2 更低时。在 HL-60with 橡黄素的文化以后, p-AMPK 被增加, Cox-2 被减少,但是 LKB1 仍然保持未改变。在有 CompoundC 的 HL-60 的文化以后, p-AMPK 被减少。在 LKB1 在之间没有重要差别橡黄素独自一个并且橡黄素 + CompoundC 组。p-AMPK 更显著地减少了,当 Cox-2 在橡黄素 + 混合物 C groupsthan 更显著地增加了时那些在橡黄素独自一个的组。一起拿,这些调查结果建议橡黄素激活独立于 LKB1 激活的 AMPK expressionin HL-60 房间,由激活 AMPK 禁止 Cox-2 表示,并且进一步调整施加它的反白血病效果的 apoptosis 的 Bcl-2-dependentpathways。 | Jie Xiao Guomin Niu Songmei Yin Shuangfeng Xie Yiqing Li Danian Nie Liping Ma Xiuju Wang Yudan Wu | 2014 | Acta Biochimica et Biophysica Sinica2014，46，5： | 2 |
| 20 | Rapid and accurate detection of carbapenem-resistance gene by isothermal amplification in Acinetobacter baumannii显示文摘Background:Acinetobacter baumannii(A.baumannii)is one of the pivotal pathogens responsible for nosocomial infections,especially in patients with low immune response,and infection with carbapenem-resistant A.baumannii has been increasing in recent years.Rapid and accurate detection of carbapenem-resistance genes in A.baumannii could be of immense help to clinical staff.Methods:In this study,a 15-μL reaction system for recombinase polymerase amplification(RPA)was developed and tested.We collected 30 clinical isolates of A.baumannii from the Burn Institute of Southwest Hospital of Third Military Medical University(Army Medical University)for 6 months and tested antibiotic susceptibility using the VITEK 2 system.A.baumannii was detected based on the blaOXA-51 gene by PCR,qPCR and 15μL-RPA,respectively.Sensitivity and specificity were evaluated.In addition,PCR and 15μL-RPA data for detecting the carbapenem-resistance gene blaOXA-23 were comparatively assessed.Results:The detection limit of the blaOXA-51 gene by 15μL RPA was 2.86 CFU/ml,with sensitivity comparable to PCR and qPCR.No positive amplification signals were detected in non-Acinetobacter isolates,indicating high specificity.However,only 18 minutes were needed for the 15μL RPA assay.Furthermore,an antibiotic susceptibility test showed that up to 90%of A.baumannii strains were resistant to meropenem and imipenem;15μL RPA data for detecting blaOXA-23 showed that only 10%(n=3)of A.baumannii isolates did not show positive amplification signals,and the other 90%of(n=27)isolates were positive,corroborating PCR results.Conclusion:We demonstrated that the new 15μL RPA assay for detecting blaOXA-23 in A.baumannii is faster and simpler than qPCR and PCR.It is a promising alternative molecular diagnostic tool for rapid and effective detection of A.baumannii and drug-resistance genes in the field and point-ofcare testing. | Shuang Liu Guangtao Huang Yali Gong Xiaojun Jin Yudan Meng Yizhi Peng Junning Zhao Xiaolu Li Qin Li | 2020 | Burns & Trauma2020，8，1： | 2 |
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