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| 1 | Erianin,a novel dibenzyl compound in Dendrobium extract,inhibits lung cancer cell growth and migration via calcium/calmodulin-dependent ferroptosis显示文摘Ferroptosis,a novel form of programmed cell death,is characterized by iron-dependent lipid peroxidation and has been shown to be involved in multiple diseases,including cancer.Stimulating ferroptosis in cancer cells may be a potential strategy for cancer therapy.Therefore,ferroptosis-inducing drugs are attracting more attention for cancer treatment.Here,we showed that erianin,a natural product isolated from Dendrobium chrysotoxum Lindl,exerted its anticancer activity by inducing cell death and inhibiting cell migration in lung cancer cells.Subsequently,we demonstrated for the first time that erianin induced ferroptotic cell death in lung cancer cells,which was accompanied by ROS accumulation,lipid peroxidation,and GSH depletion.The ferroptosis inhibitors Fer-1 and Lip-1 but not Z-VAD-FMK,CQ,or necrostatin-1 rescued erianin-induced cell death,indicating that ferroptosis contributed to erianin-induced cell death.Furthermore,we demonstrated that Ca^(2+)/CaM signaling was a critical mediator of erianin-induced ferroptosis and that blockade of this signaling significantly rescued cell death induced by erianin treatment by suppressing ferroptosis.Taken together,our data suggest that the natural product erianin exerts its anticancer effects by inducing Ca^(2+)/CaMdependent ferroptosis and inhibiting cell migration,and erianin will hopefully serve as a prospective compound for lung cancer treatment. | Peng Chen Qibiao Wu Jiao Feng Lili Yan Yitian Sun Shuiping Liu Yu Xiang Mingming Zhang Ting Pan Xiaying Chen Ting Duan Lijuan Zhai Bingtao Zhai Wengang Wang Ruonan Zhang Bi Chen Xuemeng Han Yicong Li Liuxi Chen Ying Liu Xingxing Huang Ting Jin Wenzheng Zhang Hong Luo Xiaohui Chen Yongqiang Li Qiujie Li Guohua Li Qin Zhang Lvjia Zhuo Zuyi Yang Huifen Tang Tian Xie Xiaoping Ouyang Xinbing Sui | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 42 |
| 2 | Clinical efficacy and safety of moxifloxacin versus levofloxacin plus metronidazole for community-acquired pneumonia with aspiration factors显示文摘 | Sun Tieying Sun Li Wang Rongmei Ren Xiaoping Sui Dong-jiang Pu Chun Ren Yajuan Liu Ying Yang Zhuo Li Fengzhi | 2014 | Chinese Medical Journal2014，，7： | 34 |
| 3 | A "green pathway" different from simple diffusion in soft matter: Fast molecular transport within micro/nanoscale multiphase porous systems显示文摘 | Jiantao Feng Fang Wang Xinxiao Han Zhuo Ao Quanmei Sun Wenda Hua Peipei Chen Tianwei Jing Hongyi Li Dong Han | 2014 | Nano Research2014，7，3： | 18 |
| 4 | Hawthorn Extract Alleviates Atherosclerosis through Regulating Inflammation and Apoptosis Related Factors: An Experimental Study显示文摘Objective: To determine the effects of hawthorn extract on serum lipid levels, pathological changes in aortic atherosclerosis plaque, inflammatory factors, and apoptosis-related protein and mRNA expression in apolipoprotein E gene knockout(ApoE^(-/-)) mice. Methods: Thirty-six ApoE^(-/-) mice were fed with a high-fat diet starting at the age of 8 weeks. Mice were randomly divided into 3 groups by a random number table including model group, hawthorn extract group, and simvastatin group, 12 mice in each group. Twelve 8-week-old C57BL/6 mice were fed a basic diet and served as control. The mice in the control and model groups were administered 0.2 mL saline daily, the mice in the hawthorn extract and simvastatin groups were administered with 50 mg/kg hawthorn extract or 5 mg/kg simvastatin daily for 16 weeks. After 16 weeks, plasma lipids including total cholesterol(TC), triglyceride(TG), low-density lipoprotein cholesterol(LDL-C) and high-density lipoprotein cholesterol(HDL-C) were determined by an enzymatic assay. Aortic atherosclerotic lesions were observed by light microscopy, scanning and transmission electron microscopy, respectively. Plasma levels of monocyte chemoattractant protein-1(MCP-1), interleukin-1β(IL-1β), adiponectin(APN), and hypersensitive C-reactive protein(hs-CRP) were measured by enzyme-linked immunosorbent assay(ELISA). Protein and mRNA expressions of Bax and Bcl-2 in the aorta were assessed by Western blotting and quantitative real-time polymerase chain reaction(qR T-PCR), respectively. Results: Compared to the control group, the plasma levels of TC, TG and LDL-C were significantly increased and HDL-C were significantly decreased in the model group(P<0.01). Compared to the model group, treatment with hawthorn extract significantly decreased the plasma levels of TC, TG, and LDL-C and increased the plasma level of HDL-C in ApoE^(-/-)mice(P<0.01). The levels of MCP-1, IL-1β, and hs-CRP in the model group were significantly increased and APN was significantly decreased compared with the control group(P<0.01). Compared to the model group, treatment with hawthorn extract decreased the levels of MCP-1, IL-1β, and hs-CRP and increased the APN level(P<0.01). Compared to the control group, the protein and mR NA expression of Bax in the model group were significantly increased and the expression of Bcl-2 was significantly decreased(P<0.01). Hawthorn extract also reduced the protein and mR NA expression of Bax and increased the Bcl-2 expression in the aorta(P<0.01). Conclusion: Hawthorn extract has anti-atherosclerosis and stabilizing unstable plaque effects. The mechanism may be related to the inflammation and apoptosis signaling pathways. | WANG Song-zi WU Min CHEN Ke-ji LIU Yue SUN Jing SUN Zhuo MA He LIU Long-tao | 2019 | Chinese Journal of Integrative Medicine2019，25，2： | 15 |
| 5 | Machine learning in materials genome initiative:A review显示文摘Discovering new materials with excellent performance is a hot issue in the materials genome initiative.Traditional experiments and calculations often waste large amounts of time and money and are also limited by various conditions. Therefore, it is imperative to develop a new method to accelerate the discovery and design of new materials. In recent years, material discovery and design methods using machine learning have attracted much attention from material experts and have made some progress. This review first outlines available materials database and material data analytics tools and then elaborates on the machine learning algorithms used in materials science. Next, the field of application of machine learning in materials science is summarized, focusing on the aspects of structure determination, performance prediction, fingerprint prediction, and new material discovery. Finally, the review points out the problems of data and machine learning in materials science and points to future research. Using machine learning algorithms, the authors hope to achieve amazing results in material discovery and design. | Yingli Liu Chen Niu Zhuo Wang Yong Gan Yan Zhu Shuhong Sun Tao Shen | 2020 | Journal of Materials Science & Technology2020，54，22： | 15 |
| 6 | Antioxidant and Anti-inflammatory Capacity of Ferulic Acid Released from Wheat Bran by Solid-state Fermentation of Aspergillus niger显示文摘Objective A strain of Aspergillus niger(A. niger), capable of releasing bound phenolic acids from wheat bran, was isolated. This strain was identified by gene sequence identification. The antioxidant and anti-inflammatory capacity of ferulic acid released from wheat bran by this A. niger strain(FA-WB) were evaluated. Methods Molecular identification techniques based on PCR analysis of specific genomic sequences were conducted; antioxidant ability was examined using oxygen radical absorbance capacity(ORAC), cellular antioxidant activity(CAA) assays, and erythrocyte hemolysis assays. RAW264.7 cells were used as a model to detect anti-inflammatory activity. Results The filamentous fungal isolate was identified to be A. niger. ORAC and CAA assay showed that FA-WB had better antioxidant activity than that of the ferulic acid standard. The erythrocyte hemolysis assay results suggested that FA-WB could attenuate AAPH-induced oxidative stress through inhibition of reactive oxy gen species(ROS) generation. FA-WB could significantly restore the AAPH-induced increase in intracellular antioxidant enzyme activities to normal levels as well as inhibit the intracellular malondialdehyde formation. TNF-?, IL-6, and NO levels indicated that FA-WB can inhibit the inflammation induced by lipopolysaccharide(LPS). Conclusion Ferulic acid released from wheat bran by a new strain of A. niger had good anti-inflammatory activity and better antioxidant ability than standard ferulic acid. | YIN Zhi Na WU Wen Jia SUN Chong Zhen LIU Hui Fan CHEN Wen Bo ZHAN Qi Ping LEI Zhuo Gui XIN Xuan MA Juan Juan YAO Kun MIN Tian ZHANG Meng Meng WU Hui | 2019 | Biomedical and Environmental Sciences2019，32，1： | 14 |
| 7 | Distribution of Aluminum and Fluoride in Tea Plant and Soil of Tea Garden in Central and Southwest China显示文摘The distribution of Al and F contents and the relationship between Al and F in tea plants and soils of 12 tea gardens in Central and Southwest China were investigated from October 31 to November 14, 2006. The results show that there were differences in pH, CEC, the contents of organic matter (OM), Al and F in the different soils of the tea gardens. The Al content ranged from 1196 to 7976mg/kg for old leaf, 370 to 2681mg/kg for young leaf and 285 to 525mg/kg for stem, whereas the content of F ranged from 221 to 1504mg/kg for old leaf, 49 to 602mg/kg for young leaf and 13.5 to 77.5mg/kg for stem. The concentrations of labile Al varied obviously in the different soils, but the distribution law of labile Al content for the same garden was Alexchangeable≈AlFe-Mn oxide>Alorganic>Alwater-soluble. The contents of different labile F fractions varied slightly in the different soils and the different soil layers, though the exchangeable F content was lowest among the labile F in the soils. The concentrations of Al and F in tea plants increased with increasing amount of water-soluble Al or F, especially the amount of water-soluble fractions in the soil layer of 0-20cm.The correlation between Al content and F content in the tea leaf was more significant than that in the tea stem. Furthermore, the correlation between Al content and F content in whole tea plant was strongly significant (r=0.8763, p<0.01, n=36). There were evident tendency that Al concentration increased with the increase of F concentration in different soil layers. The correlation of water-soluble Al with water-soluble F in all soils was also strongly significant (r=0.7029, p<0.01, n=34). The results may provide a proof that Al and F are jointly taken up by tea plants to some extent in natural tea gardens. | XIE Zhonglei CHEN Zhuo SUN Wentian GUO Xiaojing YIN Bo WANG Jinghua | 2007 | Chinese Geographical Science2007，17，4： | 13 |
| 8 | Key residues of the receptor binding motif in the spike protein of SARS-CoV-2 that interact with ACE2 and neutralizing antibodies显示文摘Coronavirus disease 2019(COVID-19),caused by the novel human coronavirus SARS-CoV-2,is currently a major threat to public health worldwide.The viral spike protein binds the host receptor angiotensin-converting enzyme 2(ACE2)via the receptor-binding domain(RBD),and thus is believed to be a major target to block viral entry.Both SARS-CoV-2 and SARS-CoV share this mechanism.Here we functionally analyzed the key amino acid residues located within receptor binding motif of RBD that may interact with human ACE2 and available neutralizing antibodies.The in vivo experiments showed that immunization with either the SARS-CoV RBD or SARS-CoV-2 RBD was able to induce strong clade-specific neutralizing antibodies in mice;however,the cross-neutralizing activity was much weaker,indicating that there are distinct antigenic features in the RBDs of the two viruses.This finding was confirmed with the available neutralizing monoclonal antibodies against SARS-CoV or SARS-CoV-2.It is worth noting that a newly developed SARS-CoV-2 human antibody,HA001,was able to neutralize SARS-CoV-2,but failed to recognize SARS-CoV.Moreover,the potential epitope residues of HA001 were identified as A475 and F486 in the SARS-CoV-2 RBD,representing new binding sites for neutralizing antibodies.Overall,our study has revealed the presence of different key epitopes between SARS-CoV and SARSCoV-2,which indicates the necessity to develop new prophylactic vaccine and antibody drugs for specific control of the COVID-19 pandemic although the available agents obtained from the SARS-CoV study are unneglectable. | Chunyan Yi Xiaoyu Sun Jing Ye Longfei Ding Meiqin Liu Zhuo Yang Xiao Lu Yaguang Zhang Liyang Ma Wangpeng Gu Aidong Qu Jianqing Xu Zhengli Shi Zhiyang Ling Bing Sun | 2020 | Cellular & Molecular Immunology2020，17，6： | 13 |
| 9 | Is the East Asian flora ancient or not?显示文摘The East Asian flora(EAF) is a key biodiversity hotspot for understanding the origin and evolution of Northern Hemisphere floras, but there is an ongoing debate on whether it is a museum or a cradle for seed plants. Within the EAF, two main floras, the Sino-Himalayan Flora(mainly the Rhododendron Flora) and the Sino-Japanese Flora(mainly the Metasequoia Flora), have been recognized. Previous studies suggested that the EAF is ancient and the Metasequoia Flora is older than the Rhododendron Flora. To test this hypothesis, we synthesized molecular as well as fossil data on seed plants, focusing on the biogeographical origins and historical evolution of the EAF. We compared the ages of its two constituent floras, and examined the impact of the Asian monsoon and other environmental changes on the development of EAF through meta-analysis. Our results suggest that the EAF might be relatively young, with most of its clades originating since the Miocene. The Rhododendron Flora and the Metasequoia Flora are probably of a similar age. The formation and development of the Asian monsoon might have been the main factors that have driven the evolution of EAF. In the Rhododendron Flora, the north-south mountain chains increased the concentration of species and reduced extinction, and the barriers between the east and west have resulted in species differentiation, which triggered it to become a diversity center. The EAF appears to have multiple biogeographical origins, having closely affiliated not only with other floras in the Northern Hemisphere, but also with Gondwanan floras. | Yong-Sheng Chen Tao Deng Zhuo Zhou Hang Sun | 2018 | National Science Review2018，5，6： | 13 |
| 10 | Earth surface processes and their effects on human behavior in monsoonal China during the Pleistocene-Holocene epochs显示文摘There is a wide diversity of landforms in China.The topography of three major terraces,decreasing in height stepwise from west to east,was formed by the early Miocene.With the commencement of the Great Northern Hemisphere Glaciations(GHGs)and the glacial-interglacial cycles in the Pleistocene,thick loess deposits accumulated in north China,and fluvial terraces were formed and lakes expanded and contracted in eastern and central China.The earliest evidence of hominins in China is dated to ~1.7 Ma;they occupied the monsoon-dominated region for a long interval,until the late Pleistocene,~50 ka.In this study,we investigated a large area rich in the relics and artifacts of early man.The results indicate that the early humans occupied riverine areas,especially medium-sized fluvial basins,and lake shores.Even in the relatively recent geological past,the occupation and abandonment of settlements were directly forced by the shifting of sand dune fields in the desert-loess transitional zone,which in turn was closely associated with variations in the monsoon climate and vegetation patterns.Our observations indicate that landforms were one of the main determinants of early human behavior,in that loess tableland,large alluvial plains,desert-Gobi areas,and the Tibetan Plateau,were not suitable environments for early human settlement.We infer that the early humans in China adapted their behavior to specific landforms and landform processes.The monsoon climate,which shapes the large-scale step-like pattern of fluvial landforms,promotes vegetation coverage and dominates soil formation,provides a crucial context for early human adaptation.The adaptation of early humans to earth surface processes in East Asia is investigated for the first time in this study.Future investigations will provide further information that will increase our understanding of the linkage between early human behavior and landform processes in East Asia. | LU Huayu ZHUO Haixin ZHANG Wenchao WANG Shejiang ZHANG Hongyan SUN Xuefeng JIA Xin XU Zhiwei WANG Xianyan | 2017 | Journal of Geographical Sciences2017，27，11： | 12 |
| 11 | Bedaquiline-containing regimens in patients with pulmonary multidrug-resistant tuberculosis in China:focus on the safety显示文摘Background:World Health Organization recommends countries introducing new drug and short treatment regimen for drug resistant tuberculosis(DR-TB)should develop and implement a system for active pharmacovigilance that allows for detection,reporting and management of adverse events.The aim of the study is to evaluate the frequency and severity of adverse events(AEs)of bedaquiline-containing regimen in a cohort of Chinese patients with multidrug-resistant(MDR)/extensively drug-resistant(XDR)-TB based on active drug safety monitoring(aDSM)system of New Drug Introduction and Protection Program(NDIP). | Jing-Tao Gao Juan Du Gui-Hui Wu Yi Pei Meng-Qiu Gao Leonardo Martinez Lin Fan Wei Chen Li Xie Yu Chen Hua Wang Long Jin Guo-Bao Li Pei-Lan Zong Yu Xiong Qian-Hong Wu Ming-Wu Li Xiao-Feng Yan Yan-Fang Miao Qing-Shan Cai Xin-Jie Li Da-Peng Bai Shu-Jun Geng Guo-Li Yang Pei-JunTang Yi Zeng Xiao-Hong Chen Tong-Xia Li Cui Cai Yun Zhou Ma Zhuo Jian-Yun Wang Wen-Long Guan Lin Xu Ji-Chan Shi Wei Shu Li-Li Cheng FeiTeng Yu-Jia Ning Shi-Heng Xie Yu-Xian Sun Li-Jie Zhang Yu-Hong Liu | 2021 | Infectious Diseases of Poverty2021，10，2： | 11 |
| 12 | Design of sandwich-structured ZnO/ZnS/Au photoanode for enhanced efficiency of photoelectrochemical wate splitting显示文摘We developed and demonstrated a ZnO/ZnS/Au composite photoanode with significantly enhanced photoelectrochemical water-splitting performance, con- taining a ZnS interlayer and Au nanoparticles. The solar-to-hydrogen conversion efficiency of this ZnO/ZnS/Au heterostructure reached 0.21%, 3.5 times that of pristine ZnO. The comparison of the incident photon-to-current efficiency (IPCE) and the photoresponse in the white and visible light regions further verified that the enhancement resulted from contributions of both UV and visible light. The modification of the Au NPs was shown to improve the photoelectrochemical (PEC) performance to both UV and visible light, as modification encouraged effective surface passivation and surface-plasmon- resonance effects. The ZnS interlayer favored the movement of photogenerated electrons under UV light and hot electrons under visible light, causing their injection into ZnO; this simultaneously suppressed the electron-hole recombination at the photoanode-electrolyte interface. The optimized design of the interlayer within plasmonic metal/semiconductor composite systems, as reported here, provided a facile and compatible photoelectrode configuration, enhancing the utilization efficiency of incident light for photoelectrochemical applications. | Yichong Liu Yousong Gu Xiaoqin Yan Zhuo Kang Shengnan Lu Yihui Sun Yue Zhang | 2015 | Nano Research2015，8，9： | 10 |
| 13 | Proteomic analysis of mitochondria reveals a metabolic switch from fatty acid oxidation to glycolysis in the failing heart显示文摘This work characterizes the mitochondrial proteomic profile in the failing heart and elucidates the molecular basis of mitochondria in heart failure. Heart failure was induced in rats by myocardial infarction, and mitochondria were isolated from hearts by differential centrifugation. Using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry, a system biology approach was employed to investigate differences in mitochondrial proteins between normal and failing hearts. Mass spectrometry identified 27 proteins differentially expressed that involved in energy metabolism. Among those, the up-regulated proteins included tricarboxylic acid cycle enzymes and pyruvate dehydrogenase complex subunits while the down-regulated proteins were involved in fatty acid oxidation and the OXPHOS complex. These results suggest a substantial metabolic switch from free fatty acid oxidation to glycolysis in heart failure and provide molecular evidence for alterations in the structural and functional parameters of mitochondria that may contribute to cardiac dysfunction during ischemic injury. | WANG Jun1,2, BAI Ling1,2, LI Jing3, SUN ChaoFeng1,2, ZHAO Jin1, CUI ChangCong1,2, HAN Ke1,2, LIU Yu1,2, ZHUO XiaoZhen1,2, WANG TingZhong1,2, LIU Ping1,2, FAN FenLing1,2, GUAN YouFei3 & MA AiQun1,2 1 Department of Cardiology, First affiliated hospital of medical college of Xi’an Jiaotong University, Xi’an 710061, China 2 Ion Channel Disease Laboratory, Key Laboratory of Environment and Genes Related to Diseases of Education Ministry, Xi’an 710061, China 3 Department of Physiology and Pathophysiology Key Laboratory of Molecular Cardiovascular Sciences of the Ministry of Education, Peking University Health Science Center, Beijing 100083, China | 2009 | Science China(Life Sciences)2009，52，11： | 10 |
| 14 | 晚期非小细胞肺癌免疫检查点抑制剂新型生物标志物:血液PD-L1动态检测显示文摘背景免疫检查点抑制剂(immune checkpoint inhibitors,ICIs)具有良好疗效已成为恶性肿瘤治疗领域备受瞩目的翘楚。然而,仅有小部分患者获得长期临床获益。如何选择生物标志物来筛查ICIs治疗优势人群仍是一大热点。方法收集51例晚期恶性肿瘤患者的肿瘤组织和配对血样本,进行PD-L1表达的相关性分析。40例晚期非小细胞肺癌(non-small cell lung cancer,NSCLC)患者接受ICIs治疗,2个月时检测血液PD-L1(bloodPD-L1,bPD-L1)表达的动态变化,包括PD-L1 mRNA、外泌体PD-L1(exosomalPD-L1,exoPD-L1)蛋白和可溶性PD-L1(solublePD-L1,sPD-L1)。应用R软件计算最佳截断值,用于预测最佳客观缓解(best objective response,b OR)、无进展生存期(progression-free survival,PFS)和总生存期(overall survival,OS)。结果51例不同恶性肿瘤患者中,组织PD-L1(tissuePD-L1,tPD-L1)表达阳性患者的PD-L1mRNA表达水平显著升高。40例接受ICIs治疗的晚期NSCLC患者中,bPD-L1 mRNA上调≥2.04的患者b OR、PFS和OS更佳;21例接受ICIs治疗的晚期NSCLC患者中,exoPD-L1上调≥1.86的患者临床获益更大。sPD-L1动态变化与ICIs疗效无显著相关性。PD-L1 mRNA和exoPD-L1联合检测更有利于预测ICIs治疗疗效。结论bPD-L1表达与tPD-L1表达存在正相关;晚期NSCLC患者ICIs治疗初期PD-L1 mRNA和(或)exoPD-L1表达升高,可作为评估临床获益的新型生物标志物。 | Qiao YANG Mingjing CHEN Jiaoyang GU Kai NIU Xianlan ZHAO Linpeng ZHENG Zihan XU Yongxin YU Feng LI Lingxin MENG Zhengtang CHEN Wenlei ZHUO Luping ZHANG Jianguo SUN 杨峤(译) 孙建国(译) | 2021 | 中国肺癌杂志2021，24，12： | 10 |
| 15 | Efficient manipulations of circularly polarized terahertz waves with transmissive metasurfaces显示文摘The unrestricted control of circularly polarized(CP)terahertz(THz)waves is important in science and applications,but conventional THz devices suffer from issues of bulky size and low efficiency.Although Pancharatnam-Berry(PB)metasurfaces have shown strong capabilities to control CP waves,transmission-mode PB devices realized in the THz regime are less efficient,limiting their applications in practice.Here,based on Jones matrix analysis,we design a trilayer structure(thickness of ~λ/5)and experimentally demonstrate that the structure can serve as a highly efficient transmissive meta-atom(relative efficiency of ~90%)to build PB metadevices for manipulating CP THz waves.Two ultrathin THz metadevices are fabricated and experimentally characterized with a z-scan THz imaging system.The first device can realize a photonic spin Hall effect with an experimentally demonstrated relative efficiency of ~90%,whereas the second device can generate a high-quality background-free CP Bessel beam with measured longitudinal and transverse field patterns that exhibit the nondiffracting characteristics of a Bessel beam.All the experimental results are in excellent agreement with full-wave simulations.Our results pave the way to freely manipulate CP THz beams,laying a solid basis for future applications such as biomolecular control and THz signal transportation. | Min Jia Zhuo Wang Heting Li Xinke Wang Weijie Luo Shulin Sun Yan Zhang Qiong He Lei Zhou | 2019 | Light(Science & Applications)2019，8，1： | 9 |
| 16 | Attenuated Listeria monocytogenes as a cancer vaccine vector for the delivery of CD24, a biomarker for hepatic cancer stem cells显示文摘稀释 Listeria monocytogenes (LM ) 是为癌症疫苗的交货的有希望的候选人向量。在由介绍抗原的房间的吞噬作用以后,这个细菌刺激主要 histocompatibility 建筑群(MHC ) 我和 MHC-II 小径并且导致抗原特定的 T 淋巴细胞的增长。包含复制缺乏的 LM 种类 Δ 的基因修正的新策略; dalΔ dat (Lmdd ) 被开发了表示并且藏匿人的 CD24 蛋白质。CD24 是仔细与 hepatocellular 癌(HCC ) 的 apoptosis,转移和复发被联系的肝的癌症干细胞 biomarker。在在老鼠的静脉内的管理以后, Lmdd-CD24 首先在怒气和肝被散布并且没引起严重机关损害。Lmdd-CD24 有效地增加了干扰素(IFN ) 的数字 -γ-producing CD8 + T 房间和 IFN-γ分泌物。Lmdd-CD24 也提高了 IL-4- 和 IL-10-producing T 助手 2 房间的数字。Lmdd-CD24 疫苗的功效进一步对 Hepa1-6-CD24 肿瘤被调查,它腹股沟地被接种进老鼠。Lmdd-CD24 显著地在老鼠减少了肿瘤尺寸并且增加了他们的幸存。尤其是, T 规章的房间(Treg ) 的减小数并且特定的 CD8 +T 房间活动的改进在渗入肿瘤的淋巴细胞(TIL ) 被观察。这些结果对 HCC 建议 Lmdd-CD24 疫苗的一个潜在的应用程序。 | Yu Yang Jiajie Hou Zhe Lin Han Zhuo DianyuChen Xudong Zhang Yun Chen Beicheng Sun | 2014 | Cellular & Molecular Immunology2014，11，2： | 9 |
| 17 | Electroacupuncture pretreatment exhibits antidepressive effects by regulating hippocampal proteomics in rats with chronic restraint stress显示文摘The clinical effect of electroacupuncture on depression is widely recognized. However, the signal transduction pathways and target proteins involved remain unclear. In the present study, rat models of chronic restraint stress were used to explore the mechanism by which electroacupuncture alleviates depression. Rats were randomly divided into control, model, and electroacupuncture groups. Chronic restraint stress was induced in the model and electroacupuncture groups by restraining rats for 28 days. In the electroacupuncture group, electroacupuncture pretreatment at Baihui(GV20) and Yintang(GV29) acupoints was performed daily(1 m A, 2 Hz, discontinuous wave, 20 minutes) prior to restraint for 28 days. Open field tests and body weight measurements were carried out to evaluate the depressive symptoms at specific time points. On day 28, the crossing number, rearing number, and body weights of the model group were significantly lower than those in the control group. Behavior test results indicated that rat models of depressive-like symptoms were successfully established by chronic restraint stress combined with solitary raising. On day 28, an isobaric tag for a relative and absolute quantitation-based quantitative proteomic approach was performed to identify differentially expressed proteins in hippocampal samples obtained from the model and electroacupuncture groups. The potential function of these differential proteins was predicted through the use of the Cluster of Orthologous Groups of proteins(COG) database. Twenty-seven differential proteins(uncharacteristic proteins expected) were selected from the model and electroacupuncture groups. In addition to unknown protein functions, COG are mainly concentrated in general prediction function, mechanism of signal transduction, amino acid transport and metabolism groups. This suggests that electroacupuncture improved depressive-like symptoms by regulating differential proteins, and most of these related proteins exist in nerve cells. | Zhuo Guo Ya Tu Tian-wei Guo Yun-chu Wu Xue-qin Yang Lan Sun Xin-jing Yang Wen-yue Zhang Yu Wang Xu-hui Zhang | 2015 | Neural Regeneration Research2015，10，8： | 8 |
| 18 | Two-micrometer thulium laser resection of the prostate-tangerine technique in benign prostatic hyperplasia patients with previously negative transrectal prostate biopsy显示文摘前列腺橘子技术(TmLRP-TT ) 的 2-m 铥激光切除术为良性的 prostatic 增生(BPH ) 作为最低限度地侵略的治疗被介绍了。这研究被承担与以前否定的 transrectal 前列腺活体检视为 BPH 病人的治疗估计 TmLRP-TT 的临床的功效和安全。用 TmLRP-TT 经历了外科的治疗的有以前否定的 transrectal 前列腺活体检视的 51 个病人的未来的分析从 2011 年 12 月被执行到 2013 年 12 月。外科手术前的地位,外科的细节,和 perioperative 复杂并发症被记录。后续结果在 1 和 6 个月与主观、客观的测试被评估。TmLRP-TT 成功地在所有病人被完成。吝啬的前列腺体积,起作用的持续时间,和 catheterization 时间是 93.3 ? ??????? | Jian Zhuo Hai-Bin Wei Fei Zhang Hai-Tao Liu Fu-Jun Zhao Bang-Min Han Xiao-Wen Sun Jun-Lu Shu-Jie Xia | 2017 | Asian Journal of Andrology2017，19，2： | 8 |
| 19 | A cyclodextrin-based nanoformulation achieves co-delivery of ginsenoside Rg3 and quercetin for chemo-immunotherapy in colorectal cancer显示文摘The immune checkpoint blockade therapy has profoundly revolutionized the field of cancer immunotherapy. However, despite great promise for a variety of cancers, the efficacy of immune checkpoint inhibitors is still low in colorectal cancer(CRC). This is mainly due to the immunosuppressive feature of the tumor microenvironment(TME). Emerging evidence reveals that certain chemotherapeutic drugs induce immunogenic cell death(ICD), demonstrating great potential for remodeling the immunosuppressive TME. In this study, the potential of ginsenoside Rg3(Rg3) as an ICD inducer against CRC cells was confirmed using in vitro and in vivo experimental approaches. The ICD efficacy of Rg3 could be significantly enhanced by quercetin(QTN) that elicited reactive oxygen species(ROS). To amelioratein vivo delivery barriers associated with chemotherapeutic drugs, a folate(FA)-targeted polyethylene glycol(PEG)-modified amphiphilic cyclodextrin nanoparticle(NP) was developed for co-encapsulation of Rg3 and QTN. The resultant nanoformulation(CD-PEG-FA.Rg3.QTN) significantly prolonged blood circulation and enhanced tumor targeting in an orthotopic CRC mouse model, resulting in the conversion of immunosuppressive TME. Furthermore, the CD-PEG-FA.Rg3.QTN achieved significantly longer survival of animals in combination with Anti-PD-L1. The study provides a promising strategy for the treatment of CRC. | Dandan Sun Yifang Zou Liu Song Shulan Han Hao Yang Di Chu Yun Dai Jie Ma Caitriona M.O’Driscoll Zhuo Yu Jianfeng Guo | 2022 | Acta Pharmaceutica Sinica B2022，12，1： | 8 |
| 20 | Gene editing and its applications in biomedicine显示文摘The steady progress in genome editing, especially genome editing based on the use of clustered regularly interspaced short palindromic repeats(CRISPR) and programmable nucleases to make precise modifications to genetic material, has provided enormous opportunities to advance biomedical research and promote human health. The application of these technologies in basic biomedical research has yielded significant advances in identifying and studying key molecular targets relevant to human diseases and their treatment. The clinical translation of genome editing techniques offers unprecedented biomedical engineering capabilities in the diagnosis, prevention, and treatment of disease or disability. Here, we provide a general summary of emerging biomedical applications of genome editing, including open challenges. We also summarize the tools of genome editing and the insights derived from their applications, hoping to accelerate new discoveries and therapies in biomedicine. | Guanglei Li Xiangyang Li Songkuan Zhuang Liren Wang Yifan Zhu Yangcan Chen Wen Sun Zeguang Wu Zhuo Zhou Jia Chen Xingxu Huang Jin Wang Dali Li Wei Li Haoyi Wang Wensheng Wei | 2022 | Science China(Life Sciences)2022，65，4： | 8 |
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