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| 1 | bHLH121 Functions as a Direct Link that Facilitates the Activation of FIT by bHLH IVc Transcription Factors for Maintaining Fe Homeostasis in Arabidopsis显示文摘Iron(Fe)deficiency is prevalent in plants grown in neutral or alkaline soil.Plants have evolved sophisticated mechanisms that regulate Fe homeostasis,ensuring survival.In Arabidopsis,FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR(FIT)is a crucial regulator of Fe-deficiency response.FIT is activated indirectly by basic helix-loop-helix(bHLH)IVc transcription factors(TFs)under Fed eficiency;how ever,it remains unclear which protein(s)act as the linker to mediate the activation of FIT by bHLH IVc TFs.In this study,we characterize the functions of bHLH121 and demonstrate that it directly associates with the FIT promoter.We found that loss-of-function mutations of bHLH121 cause severe Fedeficiency symptoms,reduced Feaccum ulation,and disrupted expression of genes associated with Fehomeostasis.Genetic analysis showed that FIT is epistatic to bHLH121 and FIT overexpression partially rescues the bhlh121 mutant.Further investigations revealed that bHLH IVc TFs interact with and promote nuclear accumulation of bHLH121.We demonstrated that bHLH121 has DNA-binding activity and can bind the prom oters of the FIT and bHLHlb genes,but we did not find that it has either direct transcriptional activation or repression activity tow ard these genes.Meanw hile,we found that bHLH121 functions downstream of and is a direct target of bHLH IVc TFs,and its expression is induced by Fe deficiency in a bHLH IV c-dependent manner.Taken together,these results establish that bHLH121 functions together with bHLH IVc TFs to positively regulate the expression of FIT and thus plays a pivotal role in maintaining Fe homeostasis in Arabidopsis. | Rihua Lei Yang Li Yuerong Cai Chenyang Li Mengna Pu Chengkai Lu Yujie Yang Gang Liang | 2020 | Molecular Plant2020，13，4： | 14 |
| 2 | Oryza sativa FER-LIKE FE DEFICIENCY-INDUCED TRANSCRIPTION FACTOR(OsFIT/OsbHLH156)interacts with OsIRO2 to regulate iron homeostasis显示文摘Iron(Fe)is indispensable for the growth and development of plants.It is well known that FER-LIKE FE DEFICIENCY-INDUCED TRANSCRIPTION FACTOR(FIT)is a key regulator of Fe uptake in Arabidopsis.Here,we identify the Oryza sativa FIT(also known as Osb HLH156)as the interacting partner of IRON-RELATED BHLH TRANSCRIPTION FACTOR 2(OsIRO2)that is critical for regulating Fe uptake.The OsIRO2 protein is localized in the cytoplasm and nucleus,but OsFIT facilitates the accumulation of OsIRO2 in the nucleus.Loss-of-function mutations of OsFIT result in decreased Fe accumulation,severe Fe-deficiency symptoms,and disrupted expression of Feuptake genes.In contrast,OsFIT overexpression promotes Fe accumulation and the expression of Fe-uptake genes.Genetic analyses indicate that OsFIT and OsIRO2 function in the same genetic node.Further analyses suggest that OsFIT and OsIRO2 form a functional transcription activation complex to initiate the expression of Fe-uptake genes.Our findings provide a mechanism understanding of how rice maintains Fe homeostasis. | Gang Liang Huimin Zhang Yang Li Mengna Pu Yujie Yang Chenyang Li Chengkai Lu Peng Xu Diqiu Yu | 2020 | Journal of Integrative Plant Biology2020，62，5： | 4 |
| 3 | Investigation of terahertz generation from passively Q-switched dual-frequency laser pulses 显示文摘 | ZHAO Pu SRINIVASA Ragam DING Yujie | 2011 | Optics Letters2011，36，24： | 1 |
| 4 | Recent progress on terahertz generation based on difference frequency generation:from power scaling to compact and portable sources显示文摘The progress achieved on power scaling and compact and portable THz sources is reviewed.By reversely stacking the GaP plates,the photon conversion efficiency is improved from 25% to 40% which corresponds to the maximum value.When the number of the plates is increased from four to five,the output power decreases because of back conversion.The THz generation is also investigated by mixing the two frequencies generated by a single Nd:YLF solid-state laser.The average output power reaches 1 μW.The introduction of two Nd:YLF crystals significantly improves the output power to 4.5 μW.This configuration facilitates the generation of different output frequencies. | Yujie J.Ding Pu Zhao Srinivasa Ragam Da Li Ioulia B.Zotova | 2011 | Chinese Optics Letters2011，9，11： | 1 |
| 5 | In vivo inducing collagen regeneration of biodegradable polymer microspheres显示文摘Biodegradable polymer particles have been used as dermal fillers for pre-clinical and clinical trials.The impact of material properties of polymers is very important to develop products for aesthetic medicine such as dermal fillers.Herein,eight biodegradable polymers with different molecular weights,chemical compositions or hydrophilic-hydrophobic properties were prepared and characterized for systematical study for aesthetic medicine applications.Polymer microspheres with 20-100 lmwere prepared.The in vitro degradation study showed that poly(L-lactic-co-glycolic acid)75/25 microspheres degraded the fastest,whereas poly(L-lactic acid)(PLLA)microspheres with intrinsic viscosity of 6.89([g]¼6.89)with the highest molecular weight showed the slowest degradation rate.After these microspheres were fabricated dermal fillers according to the formula of SculptraVR,they were injected subcutaneously into the back skin of rabbits.In vivo results demonstrated that the degradation rate of microspheres strongly correlated with the foreign body reaction and collagen regeneration was induced by microspheres.The microspheres with faster degradation rate induced inflammatory response and the collagen regeneration maintained in shorter time.PLLA([g]¼3.80)microsphere with a moderate molecular weight and degradation rate could strongly regenerate Type I and III collagen to maintain a long-term aesthetic medicine effect.These properties of size,morphology and degradation behavior would influence the foreign body reaction and collagen regeneration. | Yixin Zhang Hanwen Liang Qian Luo Jianlin Chen Nan Zhao Wenxia Gao Yuji Pu Bin He Jing Xie | 2021 | Regenerative Biomaterials2021，8，5： | 1 |
| 6 | Study on HPLC Fingerprint and Simultaneous Determination of Three Active Components of Dipsaci Radix Collected in Different Time显示文摘[Objectives] To establish methods of HPLC fingerprint and simultaneous determination of the three bioactive components(namely, asperosaponin VI, loganin and sweroside) of Dipsaci Radix, and explore the quality situation of this crude medicine collected in various months in autumn. [Methods] The analysis was performed on Thermo BDS Hypersil C_(18)(4.6 mm×250 mm, 5 μm) column with a mixture of acetonitrile and 0.1% phosphoric acid as mobile phase in gradient mood at a flow rate of 1.0 mL/min, the column temperature was set at 30 ℃, and the detection wavelength was 220 nm. [Results] The analysis methods for HPLC fingerprint and determination of the three components in Dipsaci Radix had been established. The results showed that 12 batches of samples, which were collected in four different places from September to November, possessed the similarities of greater than 0.976. Through the quantitative analysis of asperosaponin VI, loganin and sweroside in Dipsaci Radix, it was found that the quality variation of this herbal medicine and the different collected months of autumn showed a low correlation. [Conclusions] The established methods of HPLC characteristic fingerprint and simultaneous determination of three glycosides provided a new way for quality analysis of Dipsaci Radix. It was preliminarily indicated that collecting this plant medicine in different months of autumn would not affect its quality. | Pu CHEN Xiangluan WAN Hua ZHAO Yujie CHEN Dingrong WAN | 2019 | Medicinal Plant2019，10，2： | 1 |
| 7 | A Mg^(2+)/polydopamine composite hydrogel for the acceleration of infected wound healing显示文摘Bacterial infection is a vital factor to delay the wound healing process.The antibiotics abuse leads to drug resistance of some pathogenic bacteria.Non-antibiotic-dependent multifunctional biomaterials with accelerated wound healing performance are urgently desired.Herein,we reported a composite antibacterial hydrogel PDA-PAM/Mg^(2+)that shows excellent self-healing and tissue adhesive property,and photothermal antibacterial functions for accelerating wound healing.The gel was composed of polyacrylamide(PAM),polydopamine(PDA),and magnesium(Mg^(2+))and prepared via a two-step procedure:an alkali-induced dopamine pre-polymerization and followed radical polymerization process.The composite gel shows excellent tissue adhesiveness and Mg^(2+)-synergized photothermal antibacterial activity,inducing a survival rate of 5.29% for S.aureus and 7.06%for E.coli after near infrared light irradiation.The composite hydrogel further demonstrated efficient bacteria inhibition,enhanced wound healing and collagen deposition in a full-thickness skin defect rat model.Together,the PDA-PAM/Mg^(2+) hydrogel presents an excellent wound dressing with excellent tissue adhesion,wound healing,and antibacterial functions. | Zhaoyuan Guo Zhuangzhuang Zhang Nan Zhang Wenxia Gao Jing Li Yuji Pu Bin He Jing Xie | 2022 | Bioactive Materials2022，7，9： | 0 |
| 8 | Sample Collection,DNA Extraction,and Library Construction Protocols of the Human Microbiome Studies in the International Human Phenome Project显示文摘The human microbiome plays a crucial role in human health.In the past decade,advances in high-throughput sequencing technologies and analytical software have significantly improved our knowledge of the human microbiome.However,most studies concerning the human microbiome did not provide repeatable protocols to guide the sample collection,handling,and processing procedures,which impedes obtaining valid and timely microbial taxonomic and functional results.This protocol provides detailed operation methods of human microbial sample collection,DNA extraction,and library construction for both the amplicon sequencing-based measurements of the microbial samples from the human nasal cavity,oral cavity,and skin,as well as the shotgun metagenomic sequencing-based measurements of the human stool samples among adult par-ticipants.This study intends to develop practical procedure standards to improve the reproducibility of microbiota profiling of human samples. | Yetong Wang Ruyi Zhang Yanni Pu Danqi Wang Yanren Wang Xuemei Wu Yujie Pan Chen Luo Guoping Zhao Zhexue Quan Yan Zheng | 2023 | Phenomics2023，3，3： | 0 |
| 9 | Effects of Fertilization and Transplantation in the Neighbouring Area on Quality of Artemisia argyi Leaf显示文摘[Objectives]To make a preliminary study of the effects of the fertilizer application and transplantation from Qichun County,Hubei Province,China,to neighbouring area on the chemical substance contents and moxa yield of Artemisia argyi leaf in Qichun(AALQ).[Methods]The content of the total flavonoids in AALQ was determined by UV-Vis spectrophotometry with apigenin as reference substance.The contents of volatile oil and tannins were determined by the methods in Chinese Pharmacopoeia(2015 edition).The moxa yield was detected by the self-developed method of grinding and sifting with high-speed pulverizer.[Results]Whether applying cake fertilizer,or compound fertilizer after applying barnyard manure,the contents of total flavonoids and tannins in AALQ were reduced in varying degrees,and the content of volatile oil was changed,which had adversely affect the accumulation of effective substances in the leaves of medicinal A.argyi as a whole.The contents of related chemical substances and moxa yield of A.argyi planted in the neighbouring Xishui County from Qichun,Hubei Province were relatively high.[Conclusions]The contents of effective substances and moxa yield of AALQ were significantly reduced as a whole when applied fertilizer in planting,especially after the application of barnyard manure,compound fertilizer was added.The quality of A.argyi leaf would not be adversely affected if it was transplanted from Qichun County,Hubei Province,which is the genuine producing area,to nearby areas with similar ecological environment. | Juan WU Xiangluan WAN Dingrong WAN Yujie CHEN Rui PU | 2020 | Medicinal Plant2020，11，3： | 0 |
| 10 | Recent progress of hybrid cathode interface layer for organic solar cells显示文摘Organic solar cells(OSCs)have gained conspicuous progress during the past few decades due to the development of materials and upgrading of the device structure.The power conversion efficiency(PCE)of the single-junction device had surpassed 19%.The cathode interface layer(CIL),by optimizing the connection between the active layer and the cathode electrode,has become a momentous part to strengthen the performances of the OSCs.Simultaneously,CIL is also indispensable to illustrating the working mechanism of OSCs and enhancing the stability of the OSCs.In this essay,hybrid CILs in OSCs have been summarized.Firstly,the advancement and operating mechanism of OSCs,and the effects and relevant design rules of CIL are briefly concluded;secondly,the significant influence of CIL on enhancing the stability and PCE of OSCs is presented;thirdly,the characteristics of organic hybrid CIL and organic-inorganic hybrid CIL are introduced.Finally,the conclusion and outlook of CIL are summarized. | Jianru Wang Dan Zhou Zhentian Xu Yujie Pu Senmei Lan Fang Wang Feiyan Wu Bin Hu Yongfen Tong Ruizhi Lv Honglin Chu Lie Chen | 2024 | Journal of Energy Chemistry2024，91，4： | 0 |
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