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| 1 | Structures of SdrD from Staphylococcus aureus reveal the molecular mechanism of how the cell surface receptors recognize their ligands显示文摘Staphylococcus aureus is the most important Gram-positive colonizer of human skin and nasal passage,causing high morbidity and mortality.SD-repeat containing protein D(SdrD),an MSCRAMM(Microbial Surface Components Recognizing Adhesive Matrix Molecules)family surface protein,plays an important role in S.aureus adhesion and pathogenesis,while its binding target and molecular mechanism remain largely unknown.Here we solved the crystal structures of SdrD N2-N3 domain and N2-N3-B1 domain.Through structural analysis and comparisons,we characterized the ligand binding site of SdrD,and proposed a featured sequence motif of its potential ligands.In addition,the structures revealed for the first time the interactions between B1 domain and N2-N3 domain among B domain-containing MSCRAMMs.Our results may help in understanding the roles SdrD plays in S.aureus adhesion and shed light on the development of novel antibiotics. | Xiao Wang Jingpeng Ge Bao Liu Yulin Hu Maojun Yang | 2013 | Protein & Cell2013，4，4： | 3 |
| 2 | Gold nanoparticle-directed autophagy intervention for antitumor immunotherapy via inhibiting tumor-associated macrophage M2 polarization显示文摘Tumor-associated macrophages(TAMs),one of the dominating constituents of tumor microenvironment,are important contributors to cancer progression and treatment resistance.Therefore,regulation of TAMs polarization from M2 phenotype towards M1 phenotype has emerged as a new strategy for tumor immunotherapy.Herein,we successfully initiated antitumor immunotherapy by inhibiting TAMs M2 polarization via autophagy intervention with polyethylene glycol-conjugated gold nanoparticles(PEG-Au NPs).PEG-Au NPs suppressed TAMs M2 polarization in both in vitro and in vivo models,elicited antitumor immunotherapy and inhibited subcutaneous tumor growth in mice.As demonstrated by the m RFP-GFP-LC3 assay and analyzing the autophagy-related proteins(LC3,beclin1 and P62),PEGAu NPs induced autophagic flux inhibition in TAMs,which is attributed to the PEG-Au NPs induced lysosome alkalization and membrane permeabilization.Besides,TAMs were prone to polarize towards M2phenotype following autophagy activation,whereas inhibition of autophagic flux could reduce the M2polarization of TAMs.Our results revealed a mechanism underlying PEG-Au NPs induced antitumor immunotherapy,where PEG-Au NPs reduce TAMs M2 polarization via induction of lysosome dysfunction and autophagic flux inhibition.This study elucidated the biological effects of nanomaterials on TAMs polarization and provided insight into harnessing the intrinsic immunomodulation capacity of nanomaterials for effective cancer treatment. | Siyue Zhang Fangyuan Xie Kaichun Li He Zhang You Yin Yuan Yu Guangzhao Lu Shihao Zhang Yan Wei Ke Xu Yan Wu Hong Jin Lan Xiao Leilei Bao Can Xu Yulin Li Ying Lu Jie Gao | 2022 | Acta Pharmaceutica Sinica B2022，12，7： | 2 |
| 3 | Improved methodology for efficient establishment of the myocardial ischemia-reperfusion model in pigs through the median thoracic incision显示文摘To investigate the feasibility and effectiveness of establishing porcine ischemia-reperfusion models by ligating the left anterior descending(LAD)coronary artery,we first randomly divided 16 male Bama pigs into a sham group and a model group.After anesthesia,we separated the arteries and veins.Subsequently,we rapidly located the LAD coronary artery at the beginning of its first diagonal branch through a mid-chest incision.Then,we loosened and released the ligation line after five minutes of pre-occlusion.Finally,we ligated the LAD coronary artery in situ two minutes later and loosened the ligature 60 min after ischemia.Compared with the sham group,electrocardiogram showed multiple continuous lead ST-segment elevations,and ultrasound cardiogram showed significantly lower ejection fraction and left ventricular fractional shortening at one hour and seven days post-operation in the model group.Twenty-four hours after the operation,cardiac troponin T and creatine kinase-MB isoenzyme levels significantly increased in the model group,compared with the sham group.Hematoxylin and eosin staining showed the presence of many inflammatory cells infiltrating the interstitium of the myocardium in the model group but not in the sham group.Masson staining revealed a significant increase in infarct size in the ischemia/reperfusion group.All eight pigs in the model group recovered with normal sinus heart rates,and the survival rate was 100%.In conclusion,the method can provide an accurate and stable large animal model for preclinical research on ischemia/reperfusion with a high success rate and homogeneity of the myocardial infarction area. | Liuhua Zhou Jiateng Sun Tongtong Yang Sibo Wang Tiankai Shan Lingfeng Gu Jiawen Chen Tianwen Wei Di Zhao Chong Du Yulin Bao Hao Wang Xiaohu Lu Haoliang Sun Meng Lv Di Yang Liansheng Wang | 2023 | The Journal of Biomedical Research2023，37，4： | 1 |
| 4 | Integrated data-model-knowledge representation for natural resource entities显示文摘The unified management and planning of national or provincial natural resources distributed both aboveground and underground have become increasingly important.Accurate depictions of natural resource elements and their interactions are key to achieving integrated and systematic management of natural resources.However,current spatiotemporal data models are based only on data descriptions,attribute records,and other model knowledge of a more general basis,without intuitively describing relationships between these elements and natural resources.This paper,therefore,proposes an integrated data-model-knowledge representation model to explicitly describe the time,space,and interaction of natural resource entities through an integrated knowledge graph.First,this study constructs a conceptual model using the aspects of semantics,scale,and data-model-knowledge,thereby explicitly describing the relationships of natural resources.Second,a logical model of natural resource representation is proposed,that is integrated with time,space,attributes,and relationships.Finally,taking the management of water resources as an example,this paper realizes the meticulous presentation of the levels of detail and rich semantic relations of natural resource entities.The findings of this study lay the foundation for a more efficient,precise,and lucid perception of the distribution laws and complicated interactional relationships of natural resources,both aboveground and underground. | Yulin Ding Zhaowen Xu Qing Zhu Hankan Li Yan Luo Ying Bao Lingjun Tang Sen Zeng | 2022 | International Journal of Digital Earth2022，15，1： | 0 |
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