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您的检索式：作者名="Yunming Long"
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| 1 | Suppression or knockout of SaF/SaM overcomes the Sa-mediated hybrid male sterility in rice显示文摘Hybrids between the indica and japonica subspecies of rice(Oryza sativa) are usually sterile, which hinders utilization of heterosis in the inter-subspecific hybrid breeding. The complex locus Sa comprises two adjacently located genes, SaF and SaM, which interact to cause abortion of pollen grains carrying the japonica allele in japonica-indica hybrids. Here we showed that silencing of SaF or SaM by RNA interference restored male fertility in indica-japonica hybrids with heterozygous Sa. We further used clustered regularly interspaced short palindromic repeats(CRISPR)/Cas9-based genome editing to knockout the SaF and SaM alleles, respectively, of an indica rice line to create hybrid-compatible lines. The resultant artificial neutral alleles did not affect pollen viability and other agricultural traits, but did break down the reproductive barrier in the hybrids. We found that some rice lines have natural neutral allele Sa-n, which was compatible with the typical japonica or indica Sa alleles in hybrids. Our results demonstrate that SaF and SaM are required for hybrid male sterility, but are not essential for pollen development. This study provides effective approaches for the generation of hybrid-compatible lines by knocking out the Sa locus or using the natural Sa-n allele to overcome hybrid male sterility in rice breeding. | Yongyao Xie Baixiao Niu Yunming Long Gousi Li Jintao Tang Yaling Zhang Ding Ren Yao-Guang Liu Letian Chen | 2017 | Journal of Integrative Plant Biology2017，59，9： | 11 |
| 2 | Characterization and Molecular Mapping of a New Virescent Mutant in Rice显示文摘Plant leaves play a significant role in photosynthesis.Normal chloroplast development is critical for plant growth and yield performance.Defect of the chlorophyll in chloroplasts may cause abnormal leaf colors,such as yellow,white,or stripe.Chloroplasts have their own genomes encoding for about 100 genes that are essential for plastid protein synthesis and photosynthesis(Kanno and Hirai,1993; | Qunyu Zhang Dexing Xue Xiaoyu Li Yunming Long Xianjie Zeng Yaoguang Liu | 2014 | Journal of Genetics and Genomics2014，41，6： | 2 |
| 3 | Study on the preparation of a lTCs/7Ba radionuclide generator显示文摘 | Wang Yunming Lee Chungshin Cheng Wu- long | 1992 | Journal of Radioana- lytieal and Nuclear Chemistry1992，160，2： | 1 |
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