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您的检索式：作者名="Yvette Simon"
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| 1 | Rapid chromatographic method to decipher distinct alterations in lipid classes in NAFLD/NASH显示文摘AIM: To establish a simple method to quantify lipid classes in liver diseases and to decipher the lipid profile in p62/IMP2-2/IGF2BP2-2 transgenic mice.METHODS: Liver-specific overexpression of the insulin-like growth factor 2 mRNA binding protein p62/IMP2-2/IGF2BP2-2 was used as a model for steatosis.Steatohepatitis was induced by feeding a methioninecholine deficient diet. Steatosis was assessed histologically. For thin layer chromatographic analysis, lipids were extracted from freeze-dried tissues by hexane/2-propanol, dried, redissolved, and chromatographically separated by a two-solvent system. Dilution series of lipid standards were chromatographed, detected, andquantified. The detection was performed by either2',7'-dichlorofluoresceine or a sulfuric acid/ethanol mixture.RESULTS: Histological analyses confirmed steatosis and steatohepatitis development. The extraction,chromatographic, and detection method showed high inter-assay reproducibility and allowed quantification of the different lipid classes. The analyses confirmed an increase of triglycerides and phosphatidylethanolamine and a decrease in phosphatidylcholine in the methionine-choline deficient diet. The method was used for the first time to asses the lipid classes induced in the p62-overexpressing mouse model and showed a significant increase in all detected lipid species with a prominent increase of triglycerides by 2-fold. Interestingly, the ratio of phosphatidylcholine to phosphatidylethanolamine was decreased, as previously suggested as a marker in the progression from steatosis to steatohepatitis.CONCLUSION: The thin layer chromatography analysis allows a reliable quantification of lipid classes and provides detailed insight into the lipogenic effect of p62. | Stephan Laggai Yvette Simon Theo Ranssweiler Alexandra K Kiemer Sonja M Kessler | 2013 | World Journal of Hepatology2013，5，10： | 1 |
| 2 | Infrastructure for collaborative science and societal applications in the Columbia River estuary显示文摘满足社会需要,现代河口科学需要学科交差、合作,把发现与假设测试相结合,并且对面对地区性、全球的股东的问题应答。如此的一条途径最好与充满数据的环境的利益被进行,在从传感器和模型的信息在方便 timeframes 以内是公开地可存取的的地方。这里,我们介绍一如此的充满数据的环境的运作的基础结构, collaboratory 由沿海的边缘观察与预言和(b) 的科技中心的调查者的多机构的队在哥伦比亚河河口创造了学科交差的研究到支持(a) 进地区性的决策的科学知识的集成。运作的基础结构的核心部件是一个观察网络,一个当模特儿的系统和电子基础结构,其各个被描述。观察网络在长期的车站的一个广泛的数组上被抛锚,许多他们学科交差,并且被暂时的车站和活动平台的按需的推广补充,经常在协调领域运动。当模特儿的系统基于 finiteelement 未组织格子编码并且包括循环,沉积和生态系统过程的运作、面向过程的模拟。信息的流动通过奉献电子基础结构被管理,与地区性、国家的观察系统精通。 | Antonio M. BAPTISTA | 2015 | Frontiers of Earth Science2015，9，4： | 0 |
| 3 | Elevated free cholesterol in a p62 overexpression model of non-alcoholic steatohepatitis显示文摘AIM:To characterize how insulin-like growth factor 2(IGF2)m RNA binding protein p62/IMP2-2 promotes steatohepatitis in the absence of dietary cholesterol.METHODS:Non-alcoholic steatohepatitis(NASH)was induced in wild-type mice and in mice overexpressing p62 specifically in the liver by feeding the mice a methionine and choline deficient(MCD)diet for either two or four weeks.As a control,animals were fed a methionine and choline supplemented diet.Serum triglycerides,cholesterol,glucose,aspartate aminotransferase and alanine transaminase were determined by standard analytical techniques.Hepatic gene expression was determined by real-time reverse transcription-polymerase chain reaction.Generation of reactive oxygen species in liver tissue was quantified as thiobarbituric acid reactive substances using a photometric assay and malondialdehyde as a standard.Tissue fatty acid profiles and cholesterol levels were analyzed by gas chromatographymass spectrometry after hydrolysis.Hepatocellular iron accumulation was determined by Prussian blue staining in paraffin-embedded formalin-fixed tissue.Filipin staining on frozen liver tissue was used to quantify hepatic free cholesterol levels.Additionally,nuclear localization of the nuclear factor kappa B(NF-κB)subunit p65 was examined in frozen tissues.RESULTS:Liver-specific overexpression of the insulin-like growth factor 2 m RNA binding protein 2-2(IGF2BP2-2/IMP2-2/p62)induces steatosis with regular chow and amplifies NASH-induced fibrosis in the MCD mouse model.Activation of NF-κB and expression of NF-κB target genes suggested an increased inflammatory response in p62 transgenic animals.Analysis of hepatic lipid composition revealed an elevation of monounsaturated fatty acids as well as increased hepatic cholesterol.Moreover,serum cholesterol was significantly elevated in p62 transgenic mice.Dietary cholesterol represents a critical factor for the development of NASH from hepatic steatosis.Filipin staining revealed increased free cholesterol in p62 transgenic livers,which were not diet-derived.The m RNA levels of the rate-limiting enzyme for cholesterol synthesis 3-hydroxy-3-methyl-glutaryl-Co A reductase(HMG-Co A reductase or HMGCR)were not significantly upregulated,potentially due to increased cholesterol biosynthesis via elevated sterol regulatory element binding transcription factor 2(SREBF2)gene expression and increased irondeposition in transgenic animals.CONCLUSION:This study provides evidence that p62/IGF2BP2-2 drives the progression of NASH through elevation of hepatic iron deposition and increased production of hepatic free cholesterol. | Yvette Simon Sonja M Kessler Katja Gemperlein Rainer M Bohle Rolf Müller Johannes Haybaeck Alexandra K Kiemer | 2014 | World Journal of Gastroenterology2014，20，47： | 0 |
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