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| 1 | An efficient and safe strategy for germ cell-specific automatic excision of foreign DNA in F1 hybrid transgenic silkworms显示文摘The safety of transgenic technology is a major obstacle in the popularization and use of transgenic silkworms and their products.In sericulture,only the first filial generation(F1)hybrid eggs produced by cross-breeding Japanese and Chinese original strains are usually used for the large-scale breeding of silkworms,but this may result in uncontrolled transgene dispersal during the popularization and application of the F1 hybrid transgenic eggs.To address this issue,we developed a safe and efficient strategy using the GAL4/Upstream activating sequence(UAS)system,the FLP/flippase recognition target(FRT)system,and the gonad-specific expression gene promoters(RSHP1p and Nanosp)for the germ cell-specific automatic excision of foreign DNA in the F1 hybrid transgenic silkworms.We established 2 types of activator strains,R1p::GAL4-Gr and Nsp::GAL4-Gr,containing the testis-specific GAL4 gene expression cassettes driven by RSHP1p or Nanosp,respectively,and 1 type of effector strain,UAS::FLP-Rg,containing the UAS-linked FLP gene expression cassette.The FLP recombinase-mediated sperm-specific complete excision of FRT-flanked target DNA in the F1 double-transgenic silkworms resulting from the hybridization of R1p::GAL4-Gr and UAS::FLP-Rg was 100%,whereas the complete excision efficiency resulting from the hybridization of Nsp::GAL4-Gr and UAS::FLP-Rg ranged from 13.73%to 80.3%.Additionally,we identified a gene,sw11114,that is expressed in both testis and ovary of Bombyx mori,and can be used to establish novel gonad-specific expression systems in transgenic silkworms.This strategy has the potential to fundamentally solve the safety issue in the production of F1 transgenic silkworm eggs and provides an important reference for the safety of transgenic technology in other insect species. | Dingpei Long Rongpeng Liu Yang Huang Anyao Fu Yuli Zhang Zhanzhang Hao Qiang Li Hanfu Xu Zhonghuai Xiang Aichun Zhao | 2024 | Insect Science2024，31，1： | 0 |
| 2 | Analysis of TAP1 and TAP2 Polymorphism of Mother-Infant in Chinese Patients with Pre-Eclampsia显示文摘To analyze the polymorphism of TAP gene and the shared rates of alleles between mothers and their infants in Chinese patients with pre-eclampsia, TAP1 and TAP2 genotyping was performed by the amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) in 42 patients, 106 normal pregnant women, and their neonates. The allelic frequency of TAP and the alleles shared in maternal-fetus were compared and analyzed in the two groups. Our results showed that, with totally eight alleles of TAP1 and TAP2 examined in the samples, no significant difference was found in allelic frequencies between pre-eclampsia group and control group, as well as between mothers and their neonates. Similar finding was obtained in the comparison with shared alleles. In conclusion, our results do not support a role for the polymorphisms of TAP in the etiology of pre-eclampsia.Cellular & Molecular Immunology.2005;2(2):141-144. | ZhanZhang LitingJia LeiHou PingXiong XiongwenWu XuemeiWang YafeiHuang HangjunKe CaihongChang ShihongCui FeiliGong | 2005 | Cellular & Molecular Immunology2005，2，2： | 0 |
| 3 | Vertical zoning model of fluorite ore-bodies in Zhejiang显示文摘THK fluortite in Zhejiang Province is well known. However, the known outcrop mines have been graduallyexhausted by the exploiting over a long time. It has been a task of top priority to explore blind deposits.Study on the vertical zoning model will be very important and useful in the exploration for blind fluoriteore-bodies. 1 Genetic characteristics of fluorite deposits The fluorite deposits in Zhejiang Province are typical vein filling deposits. The minerals forming theores and the alterations of the wall rocks are all relatively simple. Studies show that the ore-forming materials (F and Ca etc. ) mainly come from the Chencai Group metamorphic rocks and the J3-K1 series vol- | ZHANG Shouting and XU Zhanzhang Chengdu Univershty of Technology, Chengdu 610059, China | 1999 | Chinese Science Bulletin1999，44，S2： | 0 |
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