|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Analogic China map constructed by DNA显示文摘In this research,a nanoscale DNA structure of analogic China map is created. The nanostructure of roughly 150 nm in diameter with a spatial resolution of 6 nm is purely constructed by folding DNA. The picture observed by atomic force microscopy (AFM) is almost identical with the de-signed shape. The DNA origami technology invented by Rothemund in 2006 is employed in the construc-tion of this shape,which has proved the capability of constructing almost any complicated shape enabled by DNA origami,and provides new bottom-up method for constructing nanostructures. | QIAN Lulu WANG Ying ZHANG Zhao ZHAO Jian PAN Dun ZHANG Yi LIU Qiang FAN Chunhai HU Jun HE Lin | 2006 | Chinese Science Bulletin2006，51，24： | 20 |
| 2 | Saponins from Panax japonicus attenuate age-related neuroinflammation via regulation of the mitogen-activated protein kinase and nuclear factor kappa B signaling pathways显示文摘Neuroinflammation is recognized as an important pathogenic factor for aging and related cognitive disorders. Mitogen-activated protein kinase and nuclear factor kappa B signaling pathways may mediate neuroinflammation. Saponins from Panax japonicus are the most abundant and bioactive members in rhizomes of Panax japonicus, and show anti-inflammatory activity. However, it is not known whether saponin from Panax japonicus has an anti-inflammatory effect in the aging brain, and likewise its underlying mechanisms. Sprague-Dawley rats were divided into control groups(3-, 9-, 15-, and 24-month-old groups) and saponins from Panax japonicus-treated groups. Saponins from Panax japonicus-treated groups were orally administrated saponins from Panax japonicus at three doses of 10, 30, and 60 mg/kg once daily for 6 months until the rats were 24 months old. Immunohistochemical staining and western blot assay results demonstrated that many microglia were activated in 24-month-old rats compared with 3-and 9-month-old rats. Expression of interleukin-1β, tumor necrosis factor-α, cyclooxygenase-2, and inducible nitric oxide synthase increased. Each dose of saponins from Panax japonicus visibly suppressed microglial activation in the aging rat brain, and inhibited expression levels of the above factors. Each dose of saponins from Panax japonicus markedly diminished levels of nuclear factor kappa B, IκBα, extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38. These results confirm that saponins from Panax japonicus can mitigate neuroinflammation in the aging rat brain by inhibition of the mitogen-activated protein kinase and nuclear factor kappa B signaling pathways. | Li-li Deng Ding Yuan Zhi-yong Zhou Jing-zhi Wan Chang-cheng Zhang Chao-qi Liu Yao-yan Dun Hai-xia Zhao Bo Zhao Yuan-jian Yang Ting Wang | 2017 | Neural Regeneration Research2017，12，11： | 17 |
| 3 | Effect of toll-like receptor 3 agonist poly I:C on intestinal mucosa and epithelial barrier function in mouse models of acute colitis显示文摘AIM To investigate potential effects of poly I:C on mucosal injury and epithelial barrier disruption in dextran sulfate sodium(DSS)-induced acute colitis.METHODS Thirty C57BL/6 mice were given either regular drinking water(control group) or 2%(w/v) DSS drinking water(model and poly I:C groups) ad libitum for 7 d. Poly I:C was administrated subcutaneously (20 μg/mouse) 2 h prior to DSS induction in mice of the poly I:C group. Severity of colitis was evaluated by disease activity index, body weight, colon length, histology and myeloperoxidase (MPO) activity, as well as the production of proinflammatory cytokines, including tumor necrosis factor-α(TNF-α), interleukin 17(IL-17) and interferon-γ (IFN-γ). Intestinal permeability was analyzed by the fluorescein isothiocyanate labeleddextran (FITC-D) method. Ultrastructural features of the colon tissue were observed under electron microscopy. Expressions of tight junction(TJ) proteins, including zo-1, occludin and claudin-1, were measured by immunohistochemistry/immunofluorescence, Western blot and real-time quantitative polymerase chain reaction(RT-qPCR).RESULTS DSS caused significant damage to the colon tissue in the model group. Administration of poly I:C dramatically protected against DSS-induced colitis, as demonstrated by less body weight loss, lower disease activity index score, longer colon length, colonic MPO activity, and improved macroscopic and histological scores. It also ameliorated DSS-induced ultrastructural changes of the colon epithelium, as observed under scanning electron microscopy, as well as FITC-D permeability. The m RNA and protein expressions of TJ protein, zo-1, occludin and claudin-1 were also found to be significantly enhanced in the poly I:C group, as determined by immunohistochemistry/immunofluorescence, Western blot and RT-q PCR. By contrast, poly I:C pretreatment markedly reversed the DSS-induced up-regulated expressions of the inflammatory cytokines TNF-α, IL-17 and IFN-γ.CONCLUSION Our study suggested that poly I:C may protect against DSS-induced colitis through maintaining integrity of the epithelial barrier and regulating innate immune responses, which may shed light on the therapeutic potential of poly I:C in human colitis. | Hong-Wei Zhao Yue-Hong Yue Hua Han Xiu-Li Chen Yong-Gang Lu Ji-Min Zheng Hong-Tao Hou Xiao-Meng Lang Li-Li He Qi-Lu Hu Zi-Qian Dun | 2017 | World Journal of Gastroenterology2017，23，6： | 5 |
| 4 | Mitochondrial DNA T7719G in tRNA-Lys gene affects litter size in Small-tailed Han sheep显示文摘Background: In farm animals, mitochondrial DNA(mtDNA) effect on economic performance remains hot-topic for breeding and genetic selection. Here, 53 maternal lineages of Small-tailed Han sheep were used to investigate the association of mitochondrial DNA variations and the lambing litter size.Results: Sequence sweeping of the mitochondrial coding regions discovered 31 non-synonymous mutations, and the association study revealed that T7719G in mt DNA t RNA-Lys gene was associated with litter size(P < 0.05),manifesting 0.29 lambs per litter between the G and T carriers. Furthermore, using the mixed linear model, we assayed the potential association of the ovine litter size and haplogroups and multiple-level mtDNA haplotypes,including general haplotypes, assembled haplotypes of electron transport chain contained sequences(H-ETC),mitochondrial respiratory complex contained sequences(H-MRC) and mitochondrial genes(H-gene, including polypeptide-coding genes, rRNA genes and tRNA genes). The strategy for assembled mitochondrial haplotypes was proposed for the first time in mtDNA association analyses on economic traits, although none of the significant relations could be concluded(P > 0.05). In addition, the nuclear major gene BMPR1B was significantly correlated with litter size in the flock(P < 0.05), however, did not interact with mtDNA T7719G mutation(P > 0.05).Conclusions: Our results highlight mutations of ovine mitochondrial coding genes, suggesting T7719G in tRNA-Lys gene be a potentially useful marker for selection of sheep litter size. | Xiaoyong Chen Dan Wang Hai Xiang Weitao Dun Dave O.H.Brahi Tao Yin Xingbo Zhao | 2017 | Journal of Animal Science and Biotechnology2017，8，3： | 4 |
| 5 | Purification of green fluorescent protein using a Two-Intein system 显示文摘 | Zhao Z L Lu W Dun BQ | 2008 | Ap-plied Microbiology and Biotechnology2008，77，： | 1 |
| 6 | A direct truncation method for finding abundant exact solutions and application to the one-dimensional higher-order Schr(o)dinger equation显示文摘 | ZHAO Dun LUO Hong-Gang WANG Shun-Jin | | 0，，24： | 1 |
| 7 | On the spaces Lp(x) (Ω) and Wm,p(x) (Ω) 显示文摘 | Fan Xianling Zhao Dun | 2001 | J Math Anal Appl2001，263，2： | 1 |
| 8 | Purification of green fluorescent protein using a two - intein system 显示文摘 | Zhao Z Lu W Dun B | 2008 | Appl Mierobiol Biot2008，77，5： | 1 |
| 9 | The Design of Power Transmission of Electric Screw Press Directly Driven by Permanent Magnet Disc Synchronous Motor显示文摘 | Liang Jin Tao Zhao Sheng Dun Zhao Yong Li Sun Zhen Yu | 2011 | Applied Mechanics and Materials2011，，86： | 1 |
| 10 | SJES14110700139313显示文摘 | Jun Zhao Huahua Dun | 2014 | Engineering Structures2014，，： | 1 |
| 11 | Purification of green fluorescent protein using a Two-Intein system显示文摘 | Zhao Z L Lu W Dun B Q | 2008 | Applied Microbiology and Biotechnology2008，77，： | 1 |
| 12 | Compact embeddings theorems with symmetry of Strauss?Lions type for the space wm,p(X) (fl)显示文摘 | FAN Xian-ling ZHAO Yuan-zhang ZHAO Dun | 2001 | J Math Anal Appl2001，255，： | 1 |
| 13 | Purification of green fluorescent protein using a two-intein system 显示文摘 | Zhao Z Lu W Dun B | 2005 | Appl Microbiol Biotechnol2005，77，5： | 1 |
| 14 | Experimental and analytical studies on the stability of structural steel tube and coupler scaffolds without X-bracing 显示文摘 | LIU Hong-bo ZHAO Qiu-hong WANG Xiao- dun | 2010 | Engineering Structures2010，32，4： | 1 |
| 15 | Sobolev embedding theorems for spaces W^k,p(x)(Ω)显示文摘 | Xianling Fan Jishen Shen Dun Zhao | 2001 | J Math Anal Appl2001，262，2： | 1 |
| 16 | On the Spaces L^p(x) (Ω) and W^m·p(x) (Ω) 显示文摘 | FAN Xian ling ZHAO Dun | 2001 | J Math Anal Appl2001，263，2： | 1 |
| 17 | On the spaces L^p(Ω) and W^k,p(x)(Ω)显示文摘 | Xianling Fan Dun Zhao | 2001 | J Math Anal Appl2001，263，2： | 1 |
| 18 | Eigenvalues of p(x)-Laplacian Dirichlet problem显示文摘 | FAN Xianling ZHANG Qihu ZHAO Dun | 2005 | J Math Anal Appl2005，302，： | 1 |
| 19 | Eigenvalues of p(x)-Laplacian Dirichlet problem显示文摘 | FAN Xianling ZHANG Qihu ZHAO Dun | 2005 | J Math Anal Appl2005，302，： | 1 |
| 20 | On the space LP(x) and wm,p(x) (O)显示文摘 | FAN Xian-ling ZHAO Dun | 2010 | J Math Anal Appl2010，263，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员