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| 1 | Analysis of genetic architecture and favorable allele usage of agronomic traits in a large collection of Chinese rice accessions显示文摘Genotyping and phenotyping large natural populations provide opportunities for population genomic analysis and genome-wide association studies(GWAS). Several rice populations have been re-sequenced in the past decade;however, many major Chinese rice cultivars were not included in these studies. Here, we report large-scale genomic and phenotypic datasets for a collection mainly comprised of 1,275 rice accessions of widely planted cultivars and parental hybrid rice lines from China. The population was divided into three indica/Xian and three japonica/Geng phylogenetic subgroups that correlate strongly with their geographic or breeding origins. We acquired a total of 146 phenotypic datasets for 29 agronomic traits under multi-environments for different subpopulations. With GWAS, we identified a total of 143 significant association loci, including three newly identified candidate genes or alleles that control heading date or amylose content. Our genotypic analysis of agronomically important genes in the population revealed that many favorable alleles are underused in elite accessions, suggesting they may be used to provide improvements in future breeding efforts. Our study provides useful resources for rice genetics research and breeding. | Xiuxiu Li Zhuo Chen Guomin Zhang Hongwei Lu Peng Qin Ming Qi Ying Yu Bingke Jiao Xianfeng Zhao Qiang Gao Hao Wang Yunyu Wu Juntao Ma Liyan Zhang Yongli Wang Lingwei Deng Shanguo Yao Zhukuang Cheng Diqiu Yu Lihuang Zhu Yongbiao Xue Chengcai Chu Aihong Li Shigui Li Chengzhi Liang | 2020 | Science China(Life Sciences)2020，63，11： | 8 |
| 2 | MicroRNA396-mediated alteration in plant development and salinity stress response in creeping bentgrass显示文摘The conserved microRNA396(miR396)is involved in plant growth,development,and abiotic stress response in multiple plant species through regulating its targets,Growth Regulating Factor(GRF)transcription factor genes.However,the role of miR396 has not yet been characterized in perennial monocot species.In addition,the molecular mechanism of miR396-mediated abiotic stress response remains unclear.To elucidate the role of miR396 in perennial monocot species,we generated transgenic creeping bentgrass(Agrostis stolonifera)overexpressing Osa-miR396c,a rice miRNA396 gene.Transgenic plants exhibited altered development,including less shoot and root biomass,shorter internodes,smaller leaf area,fewer leaf veins,and epidermis cells per unit area than those of WT controls.In addition,transgenics showed enhanced salt tolerance associated with improved water retention,increased chlorophyll content,cell membrane integrity,and Na^(+)exclusion during high salinity exposure.Four potential targets of miR396 were identified in creeping bentgrass and up-regulated in response to salt stress.RNA-seq analysis indicates that miR396-mediated salt stress tolerance requires the coordination of stress-related functional proteins(antioxidant enzymes and Na+/H+antiporter)and regulatory proteins(transcription factors and protein kinases).This study establishes a miR396-associated molecular pathway to connect the upstream regulatory and downstream functional elements,and provides insight into the miRNA-mediated regulatory networks. | Shuangrong Yuan Junming Zhao Zhigang Li Qian Hu Ning Yuan Man Zhou Xiaoxia Xia Rooksie Noorai Christopher Saski Shigui Li Hong Luo | 2019 | Horticulture Research2019，6，1： | 8 |
| 3 | Identification and characterization of rice blast resistance gene Pid4 by a combination of transcriptomic profiling and genome analysis显示文摘Map-based cloning of plant disease resistance (R) genes is time-consuming. Here, we reported the isolation of blast R gene Pid4 using comparative transcriptomic profiling and genome-wide sequence analysis. Pid4 encodes a coiled-coil nucleotide-binding site leucine-rich repeat(CC-NBS-LRR) protein and is constitutively expressed at diverse developmental stages in the rice variety Digu. The Pid4 protein is localized in both the nucleus and cytoplasm. Introduction of Pid4 into susceptible rice cultivars confers race-specific resistance to leaf and neck blast. Amino acid sequence comparison and blast resistance spectrum tests showed that Pid4 is a novel R gene, different from the previously reported R genes located in the same gene cluster. A Pid4 Indel marker was developed to facilitate the identification of Pid4 in different rice varieties. We demonstrated that a plant R gene can be quickly isolated using transcriptomic profiling coupled with genome-wide sequence analysis. | Zhixiong Chen Wen Zhao Xiaobo Zhu Chengdong Zou Junjie Yin Mawsheng Chern Xiaogang Zhou Heng Ying Xin Jiang Yongzhen Li Haicheng Liao Mengping Cheng Weitao Li Min He Jing Wang Jichun Wang Bingtian Ma Jirui Wang Shigui Li Lihuang Zhu Xuewei Chen | 2018 | Journal of Genetics and Genomics2018，45，12： | 7 |
| 4 | Three-dimensional conformal intensity-modulated radiation therapy of left femur foci does not damage the sciatic nerve显示文摘During radiotherapy to kill femoral hydatid tapeworms,the sciatic nerve surrounding the focus can be easily damaged by the treatment.Thus,it is very important to evaluate the effects of radiotherapy on the surrounding nervous tissue.In the present study,we used three-dimensional,conformal,intensity-modulated radiation therapy to treat bilateral femoral hydatid disease in Meriones meridiani.The focus of the hydatid disease on the left femur was subjected to radiotherapy(40 Gy) for 14 days,and the right femur received sham irradiation.Hematoxylin-eosin staining,electron microscopy,and terminal deoxynucleotidyl transferase-d UTP nick end labeling assays on the left femurs showed that the left sciatic nerve cell structure was normal,with no obvious apoptosis after radiation.Trypan blue staining demonstrated that the overall protoscolex structure in bone parasitized with Echinococcus granulosus disappeared in the left femur of the animals after treatment.The mortality of the protoscolex was higher in the left side than in the right side.The succinate dehydrogenase activity in the protoscolex in bone parasitized with Echinococcus granulosus was lower in the left femur than in the right femur.These results suggest that three-dimensional conformal intensity-modulated radiation therapy achieves good therapeutic effects on the secondary bone in hydatid disease in Meriones meridiani without damaging the morphology or function of the sciatic nerve. | Wanlong Xu Xibin Zhao Qing Wang Jungang Sun Jiangbo Xu Wenzheng Zhou Hao Wang Shigui Yan Hong Yuan | 2014 | Neural Regeneration Research2014，9，20： | 6 |
| 5 | From Green Super Rice to green agriculture:Reaping the promise of functional genomics research显示文摘Producing sufficient food with finite resources to feed the growing global population while having a smaller impact on the environment has always been a great challenge.Here,we review the concept and practices of Green Super Rice(GSR)that have led to a paradigm shift in goals for crop genetic improvement and models of food production for promoting sustainable agriculture.The momentous achievements and global deliveries of GSR have been fueled by the integration of abundant genetic resources,functional gene discoveries,and innovative breeding techniques with precise gene and whole-genome selection and efficient agronomic management to promote resource-saving,environmentally friendly crop production systems.We also provide perspectives on new horizons in genomic breeding technologies geared toward delivering green and nutritious crop varieties to further enhance the development of green agricul-ture and better nourish the world population. | Sibin Yu Jauhar Ali Shaochuan Zhou Guangjun Ren Huaan Xie Jianlong Xu Xinqiao Yu Fasong Zhou Shaobing Peng Liangyong Ma Dingyang Yuan Zefu Li Dazhou Chen Ruifeng Zheng Zhigang Zhao Chengcai Chu Aiqing You Yu Wei Susong Zhu Qiongyao Gu Guangcun He Shigui Li Guifu Liu Changhua Liu Chaopu Zhang Jinghua Xiao Lijun Luo Zhikang Li Qifa Zhang | 2022 | Molecular Plant2022，15，1： | 6 |
| 6 | Overproduction of OsRACK1A,an effector-targeted scaffold protein promoting OsRBOHB-mediated ROS production,confers rice floral resistance to false smut disease without yield penalty显示文摘Grain formation is fundamental for crop yield but is vulnerable to abiotic and biotic stresses.Rice grain production is threatened by the false smut fungus Ustilaginoidea virens,which specifically infects rice floral organs,disrupting fertilization and seed formation.However,little is known about the molecular mechanisms of the U.virens-rice interaction and the genetic basis of floral resistance.Here,we report that U.virens secretes a cytoplasmic effector,UvCBP1,to facilitate infection of rice flowers.Mechanistically,UvCBP1 interacts with the rice scaffold protein OsRACK1A and competes its interaction with the reduced nicotinamide adenine dinucleotide phosphate oxidase OsRBOHB,leading to inhibition of reactive oxygen species(ROS)production.Although the analysis of natural variation revealed no OsRACK1A variants that could avoid being targeted by UvCBP1,expression levels of OsRACK1A are correlated with field resistance against U.virens in rice germplasm.Overproduction of OsRACK1A restores the OsRACK1A-OsRBOHB association and promotes OsRBOHB phosphorylation to enhance ROS production,conferring rice floral resistance to U.virens without yield penalty.Taken together,our findings reveal a new pathogenic mechanism mediated by an essential effector from a flower-specific pathogen and provide a valuable genetic resource for balancing disease resistance and crop yield. | Guo-Bang Li Jia-Xue He Jin-Long Wu He Wang Xin Zhang Jie Liu Xiao-Hong Hu Yong Zhu Shuai Shen Yi-Fei Bai Zong-Lin Yao Xin-Xian Liu Jing-Hao Zhao De-Qiang Li Yan Li Fu Huang Yan-Yan Huang Zhi-Xue Zhao Ji-Wei Zhang Shi-Xin Zhou Yun-Peng Ji Mei Pu Peng Qin Shigui Li Xuewei Chen Jing Wang Min He Weitao Li Xian-Jun Wu Zheng-Jun Xu Wen-Ming Wang Jing Fan | 2022 | Molecular Plant2022，15，11： | 2 |
| 7 | Vinyl-containing silicone resin asthe crosslinking aging of heat-curable silicone rubber显示文摘 | Zhao Shigui Feng Shengyu | 2002 | Journalof Applied Polymer Science2002，83，3： | 1 |
| 8 | Vinyl-containing silicone resin as the cross linking agent of heat curable silicone rubber 显示文摘 | Zhao Shigui Feng Shengyu | 2002 | journal of Applied Polymer Science2002，83，： | 1 |
| 9 | Vinyl-containing silicone resin as the crosslinking agent of heat-curable silicone rubber 显示文摘 | Zhao Shigui Feng Shengyu | 2002 | Journal of Applied Polymer Science2002，83，14： | 1 |
| 10 | Vinyl-containing silicone resin as the erosslinking agent of heat-curable silicone rubber显示文摘 | Zhao Shigui Shengyu Feng | 2002 | Journal of Applied Polymer Science2002，83，： | 1 |
| 11 | Hydrogem-containing silicone resin as the crosslinking agent of silicone rubber显示文摘 | Zhao Shigui Feng Shengyu | 2003 | Journal of Applied Polmer Science2003，89，6： | 1 |
| 12 | Vinyl-containing silicone resin as the crosslinking agent of heat-curable silicone rubber显示文摘 | Zhao Shigui Feng Shengyu | 2002 | J Appl Polym Sci2002，83，14： | 1 |
| 13 | Hydrogen-containing silicone resin as the crosslinking agent of heat-curable silicone rubber显示文摘 | Zhao Shigui Feng Shenyu | 2003 | J Appl Polym Sci2003，88，14： | 1 |
| 14 | Chiral supramolecular 2D halogen-bonded organic frameworks constructed by post-synthetic modified cross-linking strategy显示文摘The development of preparation methods for XOFs,as a new class of organic frameworks,plays a crucial role in shaping their structures,functions,and potential applications.In this study,we presented the construction of chiral supramolecular 2D halogen-bonded organic frameworks(XOFs)through a post-synthetic modification strategy.A linear halogen-bonded organic polymer(XOP),XOP-DPBA,decorated with aldehyde groups,was initially prepared to validate the feasibility of post-synthetic modification for XOF construction and functionalization.XOP-DPBA exhibited excellent reactivity with amines,forming imine bonds.By utilizing this reactivity,a series of cross-linked 2D XOFs were efficiently prepared through post-synthetic modified cross-linking reactions.Furthermore,we successfully introduced chiralα/β-cyclodextrins(α/β-CD)into the XOF skeletons via host-guest interactions,resulting in the fabrication of chiral supramolecular 2D XOFs.These chiral XOFs displayed the induced circular dichroism(ICD)signals and assembled them into helical fibers.The post-synthetic modification strategy demonstrated its versatility and simplicity for the construction and functionalization of XOFs. | Ning Xia Jiahao Zhao Guanfei Gong Hongqiang Dong Haohu Li Jike Wang Lu Wang Shigui Chen | 2023 | Science China Chemistry2023，66，11： | 0 |
| 15 | Wide grain 4,encoding an alpha-tubulin,regulates grain size by affecting cell expansion in rice显示文摘Rice is one of the three most important food crops in the world.Increasing rice yield is an effective way to ensure food security.Grain size is a key factor affecting rice yield;however,the genetic and molecular mechanisms regulating grain size have not been fully investigated.In this study,we identified a rice mutant,wide grain 4-D(wg4-D),that exhibited a significant increase in grain width and a decrease in grain length.Histological analysis demonstrated that WG4 affects cell expansion thereby regulating grain size.MutMap-based gene mapping and complementary transgenic experiments revealed that WG4 encodes an alpha-tubulin,OsTubA1.A SNP mutation in WG4 affected the arrangement of cortical microtubules and caused a wide-grain phenotype.WG4 is located in nuclei and cytoplasm and expressed in various tissues.Our results provide insights into the function of tubulin in rice and identifies novel targets the regulation of grain size in crop breeding. | Yi Liu Lianan Guo Guoli Qu Yang Xiang Xu Zhao Hua Yuan Ting Li Liangzhu Kang Shiwen Tang Bin Tu Bingtian Ma Yuping Wang Shigui Li Weilan Chen Peng Qin | 2023 | The Crop Journal2023，11，6： | 0 |
| 16 | Nursing care of 25 children with Kawasaki disease显示文摘Objective The aim of this study is to estimate the incidence and describe the diagnosis,treatment and nursing care of Kawasaki Disease among children in Guilin city,Guangxi Province,China.The study is also designed to assess clinical aspects of retrospection,management and outcome,specifically in relation to cardiac disease.Methods To retrospectively collected the clinical data of pediatric patients with Kawasaki's disease at the Affiliated Hospital of Guilin Medical University in China,which covered a two-year period from 2015 to 2016.Results During the two-year study period,a total of 25 cases of Kawasaki disease were reported in the hospitals,comprising of 11 boys and 14 girls(male-female ratio was 1∶1.27).The seasonal distribution showed a slightly higher incidence in spring and summer.The patients’mean age of onset was 2.78 years old.The average hospitalization days of patients were 8.08(range from 5 to18days).16 Kawasaki patients were diagnosed as bronchial pneumonia.Coronary arterial abnormalities were detected during follow up by UCG in 5 cases,but no coronary artery aneurysm was found.All 25 patients recovered and were discharged from the hospital.Conclusion Early treatment and proper nursing management are key issues to prevent the occurrence of coronary artery aneurysm in Kawasaki Disease patients. | LI Shenmei GAO Yan LI Yuan ZHAO Jia LIU Chunying ZHONG Shigui | 2017 | 中西医结合护理(中英文)2017，3，12： | 0 |
| 17 | Zero-Shot Medical Image Retrieval for Emerging Infectious Diseases Based on Meta-Transfer Learning--Worldwide,2020显示文摘Introduction:Due to the increasing number of medical images,image retrieval has become an important technique for medical image analytics.Although many content-based image retrieval methods have been proposed,the retrieval of images in datasets related to emerging/new infectious diseases still remain a challenge-mostly due to the lack of historical data.As a result,the current retrieval models have limited functionality in helping doctors make accurate diagnoses of new diseases.Methods:In this paper,we propose a zero-shot retrieval model based on meta-learning and ensemble learning,which can obtain a model with stronger generalizability without using any relevant training data,and thus performs well on new types of test data.Results:The experimental results showed that the proposed method is 3% to 5% higher than the traditional method,which means that our model can retrieve relevant medical images more accurately for newly emerging data types and provide doctors with more effective assistance.Discussion:When a new infectious disease occurs,doctors can use the proposed zero-shot retrieval model to retrieve all relevant cases,quickly find the common problems of patients,find the locations of the new infections,and determine its infectivity as soon as possible.The proposed method is a new computeraided decision support technology for emerging infectious diseases. | Yuying Zhao Hanjiang Lai Jian Yin Yewu Zhang Shigui Yang Zhongwei Jia Jiaqi Ma | 2020 | China CDC weekly2020，2，52： | 0 |
| 18 | 一个转录因子的天然变异赋予水稻对稻瘟病的广谱抗性显示文摘文章简介水稻是人类重要的粮食作物,有着"水稻癌症"的稻瘟病,常年肆虐各个稻区,引起水稻大幅度减产甚至绝收,是全球粮食安全的重大隐患。一直以来,发掘和利用稻瘟病持久广谱抗性的水稻资源是水稻抗稻瘟病育种的重点和难点。 | 李伟滔 朱紫薇 Mawsheng Chern 尹俊杰 杨超 冉莉 Mengping Cheng Min He Kang Wang Jing Wang Xiaogang Zhou Xiaobo Zhu Zhixiong Chen Jichun Wang Wen Zhao Bingtian Ma Peng Qin Weilan Chen Yuping Wang Jiali Liu Wenming Wang Xianjun Wu Ping Li Jirui Wang Lihuang Zhu Shigui Li 陈学伟 | 2018 | 科学新闻2018，0，4： | 0 |
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