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| 1 | CTLA4 Silencing with siRNA Promotes Deviation of Th1/Th2 in Chronic Hepatitis B Patients显示文摘To determine whether RNA interference (RNAi) could block cytotoxic T-lymphocyte antigen 4 (CTLA4) in human lymphocytes in vitro and promote IFN-γ and IL-2 secretions, three small interfering RNAs (siRNAs) were selected based on target specificity sequences of human CTLA4 and transfected into human lymphocytes of chronic HBV patients. As a result, the expression of human CTLA4 mRNA was efficiently suppressed by all the three siRNAs. Compared with negative control (siRNA-co), siRNA-1 inhibited the expression of CTLA4 most efficiently and was used in the further study. The expressions of IFN-γ and IL-2 were upregulated and the level of IL-4 was almost unchanged in lymphocytes transfected with siRNA-1 compared with the blank control. These results indicated that siRNA-1 led to IFN-γ and IL-2 secretions, which is a main response of Th1/Th2. In a conclusion, RNAi significantly suppressed the expression of human CTLA4 mRNA in human lymphocytes in vitro, and could induce Th1/Th2 response. It could be a new therapeutic strategy for chronic HBV infection. | Yongsheng Yu Hao Wu Zhenghao Tang Guoqing Zang | 2009 | Cellular & Molecular Immunology2009，6，2： | 14 |
| 2 | Dendritic cell-based vaccination with lentiviral vectors encoding ubiquitinated hepatitis B core antigen enhances hepatitis B virus-specific immune responses in vivo显示文摘肝炎 B 病毒(HBV ) 的活动特定的细胞毒素的 T 淋巴细胞(CTL ) 戏在 HBV 的清理的一个占优势的角色。树枝状的房间(DC ) 是关键介绍抗原的房间并且在有免疫力的回答的开始起一个重要作用。我们以前验证了有效地编码 ubiquitinated 肝炎 B 核心抗原(LV-Ub-HBcAg ) 的那 lentiviral 向量导致成熟的 transduced DC,和成熟 DC 高效地导致了 T 房间极化到 Th1 并且产生了 HBcAg 特定的 CTL 前 vivo。在这研究,在 BALB/c 老鼠的 LV-Ub-HBcAg 的 HBV 特定的有免疫力的回答(H-2K d ) 被评估。LV-Ub-HBcAg 的直接注射增加了 cytokines IL-2 和 IFN- 的生产,这被显示出,得到了强壮的抗体回答,并且显著地产生了 IFN-+ 有在 BALB/c 老鼠的 HBV 特定的 CTL 回答的 CD8 + T 房间。另外, LV-Ub-HBcAg 的直接注射导致了有势力 anti-HBV 免疫者回答,类似于得到由的那些在 vitro-transduced DC。在结论,基于 DC 的治疗学的疫苗的 LV-Ub-HBcAg 得到了特定的抗体免疫者回答并且在 vivo 导致了柔韧的特定的 CTL 活动。 | Shenglan Dai Meng Zhuo Linlin Song Xiaohua Chen Yongsheng Yu Zhenghao Tang Guoqing Zang | 2015 | Acta Biochimica et Biophysica Sinica2015，47，11： | 9 |
| 3 | Delivery of Tapasin-modified CTL epitope peptide via cytoplasmic transduction peptide induces CTLs by JAK/STAT signaling pathway in vivo显示文摘特定的细胞毒素的 T 淋巴细胞(CTL ) 玩的肝炎 B 病毒(HBV ) 在病毒的控制和清理的一个重要角色。最近的研究阐明了那 Tapasin, endoplasmic 蜂窝胃女伴,是看起来在装载肽的过程必要的一个著名分子。Janus kinase/signal 变换器和小径玩的抄写(JAK/STAT ) 的使活跃之物在有免疫力的反应的一个重要角色规定和 cytokines 分泌物。我们以前证实了熔化蛋白质 CTP-HBcAg 18-27-Tapasin 能便于骨头髓的成熟导出的树枝状的房间并且在 vitro 提高特定的 CTL 回答,它可能与 JAK/STAT 发信号的激活被联系小径。为了进一步探索 JAK/STAT 发信号小径是否参予了特定的有免疫力的回答,由我们压制了的 CTP-HBcAg 18-27-Tapasin, 调停了有在 vivo 的药理学禁止者(AG490 ) 的 JAK/STAT 小径。我们的研究证明 IFN-+-CD8+ T 房间的数字在被 AG490 堵住以后与另外的组相比显著地被减少。IFN-+-CD4+ T 房间和 IL-2-CD4 + T 房间的百分比也被减少。而且, Jak2, Tyk2, STAT1,和 STAT4 的更低的表示层次在 AG490 被检测组。另外,象 Th1 一样 cytokines 的分泌物层次被减少,更弱的特定的 T 房间回答在 AG490 组被观察。而且,在这条小径在 HBV 转基因的老鼠被禁止以后,在浆液的 HBV DNA 和 HBsAg 的层次和在肝纸巾的 HBsAg 和 HBcAg 的表示层次被提高。这些结果证明表明小径的 JAK/STAT 参予 CTP-HBcAg 18-27-Tapasin 导致的 Th1 面向的有免疫力的反应,这可能为 HBV 免疫疗法提供一个理论基础。 | Shanshan Wu Xiaohua Chen Yuyan Tang Yi Zhang Dan Li Jie Chen Jieling Wang Zhenghao Tang Guoqing Zang Yongsheng Yu | 2018 | Acta Biochimica et Biophysica Sinica2018，50，2： | 6 |
| 4 | The modification of Tapasin enhances cytotoxic T lymphocyte activity of intracellularly delivered CTL epitopes via cytoplasmic transduction peptide显示文摘以前的研究证明了基于肝炎 B 病毒(HBV ) 的改进,特定的细胞毒素的 T 淋巴细胞(CTL ) 活动可以导致的治疗学的疫苗在感染 HBV 的个人的病毒的清理。endoplasmic 蜂窝胃(嗯) 女伴 Tapasin 在主要 histocompatibility 起一个重要作用建筑群(MHC ) 一级汇编并且由允许更多的肽是进 ER 的 translocated 提高特定的 MHC 班我限制 CTL 活动。联合核心抗原(HBcAg ) CTL epitope,细胞质的 transduction 肽(CTP ) 的渗透房间的性质,和女伴 Tapasin 可以得到的肝炎 B 的特性柔韧的特定的 HBV 有免疫力的回答。在现在的学习,我们在 vitro 证实了 CTP-HBcAg1827-Tapasin 熔化蛋白质的细胞质的本地化偏爱并且在支持骨头上评估了效果导出髓的树枝状的房间(BMDC ) 成熟和提高的 T 房间反应到产生特定的 CTL。我们的结果证明那 CTP-HBcAg1827-Tapasin 熔化蛋白质不能仅仅确切并且有效地渗透进细胞质提高 Tapasin 表示,而且增加表面分子(CD80, CD83, CD86,和 MHC-I ) 和 DC 的 cytokine (IL-12p70 ) 的分泌物的表示。而且,与上述熔化蛋白质对待的 DC 显著地与控制组相比在 vitro, IFN-+CD8+ T 房间的百分比和特定的 CTL 回答增加了增殖的 T 房间的 cytokine 分泌物。在结论, Tapasin 的修正能提高经由细胞内部的交货指向抗原到 DC 的演讲并且高效地得到特定的 CTL 有免疫力的回答。 | Xiaohua Chen Honghong Liu Zhenghao Tang Yongsheng Yu Guoqing Zang | 2013 | Acta Biochimica et Biophysica Sinica2013，45，3： | 6 |
| 5 | Enhancement of cytotoxic T lymphocyte activity by dendritic cells loaded with Tat-protein transduction domain-fused hepatitis B virus core antigen显示文摘人的免疫不全 virus-1-Tat 蛋白质的蛋白质 transduction 领域(PTD ) 有唯一的力量渗透细胞的膜。综合 Tat-PTD 47 的顺序 57 和肝炎 B 病毒核心抗原(HBcAg ) ,我们拼接这些序列并且连接了熔化基因进 pMAL-c2x 向量。熔化蛋白质被亲密关系层析净化并且与骨头搏动了导出髓的树枝状的房间(DC ) ,和 recombinant 蛋白质的 transduction 被免疫检测荧光抗体试金。当 recombinant HBcAg 在房间的表面上被检测时,结果证明 recombinant PTD-HBcAg 能渗透细胞质进 DC。DC 表面分子的百分比 CD86 和主要 histocompatibility 建筑群 II,和 recombinant PTD-HBcAg 导致的 cytokine (IL-12p70 ) 的生产例如 CD80,比 recombinant HBcAg 或肿瘤坏死 factor-α 导致的那些显著地高。与 PTD-HBcAg 对待的 DC 导致了 T 房间区分进特定的细胞毒素的 T 淋巴细胞(CTL ) 并且提高了杀死反应的 CTL。在结论,表示并且净化的 PTD-HBcAg 熔化蛋白质能通过血浆膜渗透进房间,支持 DC 成熟,并且提高 T 房间反应高效地产生 HBcAg 特定的 CTL。 | Xiaohua Chen Yongsheng Yu Qingchun Pan Zhenghao Tang Jinchao Han Guoqing Zang | 2008 | Acta Biochimica et Biophysica Sinica2008，40，12： | 4 |
| 6 | The delivery of HBcAg via Tat-PTD enhances specific immune response and inhibits Hepatitis B virus replication in transgenic mice显示文摘 | Xiaohua Chen Jinglan Lai Qingchun Pan Zhenghao Tang Yongsheng Yu Guoqing Zang | 2010 | Vaccine2010，，23： | 1 |
| 7 | Spectroelectrochemistry for electroreduction of p-benzoquinone in unbuffered aqueous solution 显示文摘 | Tang Yanhui Wu Yirong Wang Zhenghao | 2001 | J Electrochem Soc2001，148，： | 1 |
| 8 | THE ADSORPTION BEHAVIORS OF 2,6-DICHLOROPHENOL ONTO HYPERCROSSLINKED RESINS显示文摘 | WANG Jingping LI Zhixia ZHANG Gencheng FEI Zhenghao TANG Shuhe | 2006 | Chinese Journal of Reactive Polymers2006，15，2： | 0 |
| 9 | SEL1L preserves CD8^(+) T-cell survival and homeostasis by fine-tuning PERK signaling and the IL-15 receptor-mediated mTORC1 axis显示文摘SEL1L-mediated endoplasmic reticulum-associated degradation(ERAD)plays critical roles in controlling protein homeostasis by degrading misfolded or terminal unfolded proteins.However,it remains unclear how SEL1L regulates peripheral T-cell survival and homeostasis.Herein,we found that SEL1L deficiency led to a greatly reduced frequency and number of mature T cells,which was further validated by adoptive transfer experiments or bone marrow chimera experiments,accompanied by the induction of multiple forms of cell death.Furthermore,SEL1L deficiency selectively disrupted naïve CD8+T-cell homeostasis,as indicated by the severe loss of the naïve T-cell subset but an increase in the memory T-cell subset.We also found that SEL1L deficiency fueled mTORC1/c-MYC activation and induced a metabolic shift,which was largely attributable to enhanced expression of the IL-15 receptorαandβchains.Mechanistically,single-cell transcriptomic profiling and biochemical analyses further revealed that Sel1l−/−CD8+T cells harbored excessive ER stress,particularly aberrant activation of the PERK-ATF4-CHOP-Bim pathway,which was alleviated by supplementing IL-7 or IL-15.Importantly,PERK inhibition greatly resolved the survival defects of Sel1l−/−CD8+T cells.In addition,IRE1αdeficiency decreased mTORC1 signaling in Sel1l−/−naïve CD8+T cells by downregulating the IL-15 receptorαchain.Altogether,these observations suggest that the ERAD adaptor molecule SEL1L acts as an important checkpoint for preserving the survival and homeostasis of peripheral T cells by regulating the PERK signaling cascade and IL-15 receptor-mediated mTORC1 axis. | Yafeng Gao Wenhui Li Zhenghao Wang Cangang Zhang Yaping He Xiaowei Liu Kexin Tang Weiguo Zhang Qiaoming Long Yong Liu Jinping Zhang Baojun Zhang Lianjun Zhang | 2023 | Cellular & Molecular Immunology2023，20，10： | 0 |
| 10 | USP9x promotes CD8^(+)T-cell dysfunction in association with autophagy inhibition in septic liver injury显示文摘Sepsis is a life-threatening condition manifested by concurrent inflammation and immunosuppression.Ubiquitin-specific peptidase 9,X-linked(USP9x),is a USP domain-containing deubiquitinase which is required in T-cell development.In the present study,we investigate whether USP9x plays a role in hepatic CD8^(+)T-cell dysfunction in septic mice.We find that CD8^(+)T cells are decreased in the blood of septic patients with liver injury compared with those without liver injury,the CD4/CD8 ratio is increased,and the levels of cytolytic factors,granzyme B and perforin are downregulated.The number of hepatic CD8^(+)T cells and USP9x expression are both increased 24 h after cecal ligation and puncture-induced sepsis in a mouse model,a pattern similar to liver injury.The mechanism involves promotion of CD8^(+)T-cell dysfunction by USP9x associated with suppression of cell cytolytic activity via autophagy inhibition,which is reversed by the USP9x inhibitor WP1130.In the in vivo studies,autophagy is significantly increased in hepatic CD8^(+)T cells of septic mice with conditional knockout of mammalian target of rapamycin.This study shows that USP9x has the potential to be used as a therapeutic target in septic liver injury. | Lulu Sheng Juntao Chen Yiqing Tong Yi Zhang Qiming Feng Zhenghao Tang | 2022 | Acta Biochimica et Biophysica Sinica2022，54，12： | 0 |
| 11 | Urotensin Ⅱ increases endothelin production by vascular smooth muscle cells in rats显示文摘The aim of this study was to observe the effects of urotensin Ⅱ (UII) on production of endothelin (ET) in rat aortic vascular smooth muscle cells (VSMC). Cultured VSMCs incubated with various concentrations of UII were used to measure the YSMC 3H-TdR incorporation, the amount of ET mRNA and ET production in VSMCs. In this work we found that UII (10-10-10-8 mol/L) promoted VSMC 3H-TdR incorporation (47%-83%, P<0.01) and increased the amount of ET mRNA by 17.1% (P < 0.05) to 112.8% (P < 0.01), respectively, in a concentration dependent manner compared with control. After 4 and 8 h incubation, 10-10-10-8 mol/L of UII elevated the ET synthesis and release in a concentration dependent manner. After 4 h incubation, the content of ET in medium was 4.9, 5.36 and 7.12 pg/mL (P < 0.01). After 8 h incubation, the ET content released from VSMCs was 12.6, 12.07 and 17.17 pg/mL (P < 0.01). In addition, it was found that BQ123, a specific ETA receptor antagonist, obviously decreased the VSMC DNA synthesis induced by | Ql Yongfen, BU Dingfang, YANG Jun, ZHANG Zhaokang, NIU Dadi, SHI Yanrong, ZHANG Zhenghao, TANG Chaoshu & DU JunbaoInstitute of Cardiovascular Diseases, The First Hospital of Peking University, Beijing 100083, China Department of Physiology, Health Science | 2002 | Chinese Science Bulletin2002，47，12： | 0 |
| 12 | Mold-free self-assembled scalable microlens arrays with ultrasmooth surface and record-high resolution显示文摘Microlens arrays(MLAs)based on the selective wetting have opened new avenues for developing compact and miniaturized imaging and display techniques with ultrahigh resolution beyond the traditional bulky and volumetric optics.However,the selective wetting lenses explored so far have been constrained by the lack of precisely defined pattern for highly controllable wettability contrast,thus limiting the available droplet curvature and numerical aperture,which is a major challenge towards the practical high-performance MLAs.Here we report a mold-free and self-assembly approach of mass-production of scalable MLAs,which can also have ultrasmooth surface,ultrahigh resolution,and the large tuning range of the curvatures.The selective surface modification based on tunable oxygen plasma can facilitate the precise pattern with adjusted chemical contrast,thus creating large-scale microdroplets array with controlled curvature.The numerical aperture of the MLAs can be up to 0.26 and precisely tuned by adjusting the modification intensity or the droplet dose.The fabricated MLAs have high-quality surface with subnanometer roughness and allow for record-high resolution imaging up to equivalently 10,328 ppi,as we demonstrated.This study shows a cost-effective roadmap for mass-production of high-performance MLAs,which may find applications in the rapid proliferating integral imaging industry and high-resolution display. | Zhihao Liu Guangwei Hu Huapeng Ye Miaoyang Wei Zhenghao Guo Kexu Chen Chen Liu Biao Tang Guofu Zhou | 2023 | Light(Science & Applications)2023，12，7： | 0 |
| 13 | THE ADSORPTION BEHAVIORS OF TOLUENE ON HYPERCROSSLINKED RESINS显示文摘Investigation of the adsorption characteristics of toluene on two hypercrosslinked resins. The static and the kinetic adsorption were studied. The equilibrium adsorption date was fitted to freundlich adsorption isotherm models to evaluate the model parameters. The enthalpy, free energy, entropy are indicative of an exothermic, a spontaneous and disorder decreasing process. Experimental results show that the two adsorbents are better than the Amberlite XAD-4 for removing the toluene in aqueous solutions. | WANG Jingping FEI Zhenghao TANG Shuhe | 2005 | Chinese Journal of Reactive Polymers2005，14，1： | 0 |
| 14 | Swaying Tree Simulation by Slicing Partition显示文摘The key of swaying tree simulation is to capture the essence of the int eraction bet ween wind and tree,which has not received much attention from scholars.In this paper,we present a method for Simula ting swaying tree based on slicing partition・The tree is divided into multiple'slices',and then jointly moved to Simulate the tree swaying in wind.By slicing partition,the motion of any slice is independent of the others concurrently,which provides a parallel spatial topology for real-time calculation.Moreover,based on the fact that the wind will blow to a tree partly rat her t han the whole,partial swayings of the tree are also Simula ted.The experimentai results show the correctness and effectiveness of our method. | TANG Jing WANG Yinghui WANG Ningna NING Xiaojuan LYU Ke SUI Liansheng SHI Zhenghao | 2020 | Chinese Journal of Electronics2020，29，5： | 0 |
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