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| 1 | Expression of IGF-II in early experimental hepatocellular carcinomas and its significance in early diagnosis显示文摘AIM: To investigate the serum level and expression of insulin growth factor II (IGF-II) in liver tissues of rats with early experimental hepatocellular carcinomas (HCC) and its significance in early diagnosis.METHODS: Early experimental hepatocellular carcinomas were induced by diethylnitrosamine (DENA) in 180 male SD rats. Another 20 male SD rats served as control. The IGF-IIserum level was measured by ELISA. Immunohistochemistry and electron microscopic immunohistochemistry were usedto observe the expression of IGF-II in normal and tumor liver tissues and its ultrastructural location in malignant hepatocytes. The expressions of IGF-II in human hepatoma cell lines HepG2, SMMC7721 and human embryonic liver cell line L-02 were measured by immunocytochemistry. IGF-II mRNA level was studied by in situ hybridization.RESULTS: IGF-II was expressed in the cytoplasm of both sinusoidal cells in paracancerous cirrhotic liver tissue and malignant hepatocytes in early experimental HCC tissues.Gold particles were seen on the rough endoplasrnic reticulum and the mitochondrion in malignant hepatocytes. IGF-II was expressed in the human hepatoma cell lines. The mRNA level of IGF-II was higher in rat liver tumor tissue than in normal rat liver tissue. The serum IGF-II level of the early experimental HCC group was 34.67±10.53 ng.ml^-1 and that of the control group was 11.75±5.84 ng.ml^-1. The rank sum test was used for statistical analysis. There was a significant difference between the two groups (P<0.01).CONCLUSION: During the induction of early experimental HCC by DENA, IGF-II may promote hepatocytic proliferation via a paracrine mechanism in the pre-cancerous stage. When hepatocytes are transformed into malignant cells, they may secrete IGF-II and promote malignant cell proliferation by an autocrine mechanism. IGF-II may be a possible biological marker in the early diagnosis of HCC. | ZhengWang You-BingRuan YangGuan Sheng-HongLiu1 | 2003 | World Journal of Gastroenterology2003，9，2： | 24 |
| 2 | Peroxisome proliferator-activated receptor gamma inhibits hepatic fibrosis in rats显示文摘BACKGROUND:Hepatic fibrosis is a necessary step in the development of hepatic cirrhosis.In this study we used lentiviral vector-mediated transfection technology to evaluate the effect of peroxisome proliferator-activated receptor gamma(PPAR-γ) on rat hepatic fibrosis. METHODS:Hepatic fibrosis in rats was induced by CCl4 for 2 weeks(early fibrosis)and 8 weeks(sustained fibrosis).The rats were randomly divided into four groups:normal control, fibrosis,blank vector,and PPAR-γ.They were infected with the recombinant lentiviral expression vector carrying the rat PPAR-γgene by portal vein injection.The liver of the rats was examined histologically and hydroxyproline was assessed.In vitro primary hepatic stellate cells(HSCs)were infected with the recombinant lentiviral expression vector carrying the rat PPAR-γgene.The status of HSC proliferation was measured by the MTT assay.The protein levels of PPAR-γ,α-smooth muscle actin(α-SMA)and type I collagen expression were evaluated by the Western blotting method. RESULTS:In vitro studies revealed that expression of PPAR-γ inhibited expression ofα-SMA and type I collagen in activated HSCs(P<0.01)as well as HSC proliferation(P<0.01).In vivo experiments indicated that in the early hepatic fibrosis group,the hydroxyproline content and the level of collagen I protein in the liver in the PPAR-γtransfected group were not significantly different compared to the hepatic fibrosis group and the blank vector group;whereas the expressions of PPAR-γ andα-SMA were different compared to the hepatic fibrosis group(P<0.01).In the sustained hepatic fibrosis group,there were significant differences in the hydroxyproline content and the expression of PPAR-γ,α-SMA,and type I collagen between each group.CONCLUSION:PPAR-γcan inhibit HSC proliferation and hepatic fibrosis,and suppressα-SMA and type I collagen expression. | ZhengWang,Jia-Peng Xu,Yong-Chao Zheng,Wei Chen,Yong-Wei Sun,Zhi-YongWu and Meng Luo Department of General Surgery,Renji Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200127,China | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，1： | 18 |
| 3 | Rapid reductive degradation of azo dyes by a unique structure of amorphous alloys显示文摘An Fe-based amorphous alloy was found to significantly enhance the reductive degradation of an azo dye in aqueous solution. The surface-area normalized rate constant was up to 2.0 L m-2 min-1 at room temperature,an order of magnitude larger than any previously reported value. The activation energy of the degradation process was calculated according to the Arrhenius equation,and a much lower value than those previously reported was obtained. Valence band measurement indicated that a depressed valence band maximum and a widened empty band were established in the amorphous ribbon. The unique structure of the amorphous alloy is important-it may reduce the activation energy of the degradation process and enhance the activity of the electrons,thus accelerating the degradation process. | ZHANG ChangQin ZHU ZhengWang ZHANG HaiFeng HU ZhuangQi | 2011 | Chinese Science Bulletin2011，56，36： | 10 |
| 4 | Hierarchical magnetic core-shell nanostructures for microwave absorption:Synthesis,microstructure and property studies显示文摘Core-shell nanostructures have attracted considerable attention in the past decades because of their fundamental scientific significance and many technological applications.Recently,it has been reported that the core-shell nanostructures with advanced compositions and complicated morphologies show great potential as high-performance microwave absorbers due to their unique properties,such as large surface areas,multi-functionalities and synergistic effects between the interior core and outer shell.This review article focuses on the recent progress in synthesis and characterization of hierarchical magnetic core-shell nanostructures for microwave absorption applications based on our own work.In addition,several future trends in this field for next-generation microwave absorbers are discussed. | LIU JiWei XU JunJie LIU ZhengWang LIU XiaLin CHE RenChao | 2014 | Science China Chemistry2014，57，1： | 8 |
| 5 | Nest predators, nest-site selection and nest success of the Emei Shan Liocichla(Liocichla omeiensis), a vulnerable babbler endemic to southwestern China显示文摘Background: The Emei Shan Liocichla(Liocichla omeiensis) is a globally vulnerable babbler, endemic to southwestern China. We investigated its nest predators, nest-site selection and nest success at the Laojunshan National Nature Reserve in Sichuan, China in order to identify the precise nesting-habitat requirements of the species, and to test whether the nest-site-selection cues, preferred by the Emei Shan Liocichla, are positively associated with nest success.Methods: We used infrared cameras to determine nest predators. We compared the microhabitat attributes between nest and random sites, as well as successful and failed nests. We used Binary Logistic Regression to determine the most important variables affecting nest-site selection of the Emei Shan Liocichla. We used the nest survival analysis in Program MARK to estimate daily nest survival rates(DSR). Nest success was calculated using the Mayfield method.Results: In total 56 nests were found. The DSR for all nests that contained at least one egg was 0.9564 ± 0.0091(95 % CI 0.9346–0.9711)(n = 40), while the total nest success was 27.5 %. We identified four categories of predators in 10 nest predation events, i.e. squirrels(n = 5), snakes(n = 3), raptors(n = 1) and wasps(n = 1). We found that:(1) nest predation was the primary reason for nest failure of the Emei Shan Liocichla,(2) tree cover, bamboo cover, liana abundance and distance to forest edge or gap were the most important variables affecting nest-site selection of this species, and(3) the nest-site-selection variables we measured appeared not to be positively associated with nest success.Conclusions: Our findings suggest that the Emei Shan Liocichla tended to select nest sites near forest edges or gaps with good concealment and that nest-site selection by this species was nonrandom but not necessarily adaptive. Reducing forest-edge development and protecting bamboo stands should be effective for conservation of this species. | Yiqiang Fu Benping Chen Simon D.Dowell Zhengwang Zhang | 2016 | Chinese Birds2016，，3： | 6 |
| 6 | Higher levels of circulating monocyte-platelet aggregates are correlated with viremia and increased sCD 163 levels in HIV- 1 infection显示文摘单核白血球血小板总数(MPA ) 的增加的层次被报导高度与心血管的事件被相关。在这研究,在在 MPA 层次, HIV-1 viremia 和单核白血球激活之间的不同单核白血球子集和协会的 MPA 层次在 HIV-1 感染期间被评估。结果证明在所有三个单核白血球子集的 MPA 的百分比比在健康控制在 HIV-1-infected 题目是更高的,并且与血浆被联系在非古典、中间的单核白血球子集的病毒的负担。sCD14 和 sCD163 的血浆层次是在 HIV-1 感染的 upregulated 并且断然与病毒的负担被联系并且否定地把计数与 CD4 联系了。P-selectin glycoprotein ligand-1 (PSGL-1 ) 被显示被表示在显著地所有三个单核白血球子集上的底层并且否定地与 sCD163 水平被相关。MPA 水平与血浆 sCD163 的层次被相关,但是否定地在所有三个单核白血球子集上与 CD163 和 PSGL-1 相关。提高的有免疫力的激活地位与增加的 MPA 形成被相关,位于在单核白血球激活和 MPA 形成之间的潜在的相互作用下面。这个相互作用可能与在感染 HIV-1 的病人的更高的 thromboembolic 风险有关。 | Hua Liang Zhaojun Duan Dan Li Dongliang Li ZhengWang Li Ren Tao Shen Yiming Shao | 2015 | Cellular & Molecular Immunology2015，12，4： | 5 |
| 7 | Rapid decolorization of Acid Orange Ⅱ aqueous solution by amorphous zero-valent iron显示文摘Some problems including low treatment capacity,agglomeration and clogging phenomena,and short working life,limit the application of pre-treatment methods involving zero-valent iron (ZVI).In this article,ZVI was frozen in an amorphous state through a melt-spinning technique,and the decolorization effect of amorphous ZVI on Acid Orange II solution was investigated under varied conditions of experimental variables such as reaction temperature,ribbon dosage,and initial pH.Batch experiments suggested that the decolorization rate was enhanced with the increase of reaction temperature and ribbon dosage,but decreased with increasing initial solution pH.Kinetic analyses indicated that the decolorization process followed a first order exponential kinetic model,and the surface-normalized decolorization rate could reach 2.09 L/(m 2 ·min) at room temperature,which was about ten times larger than any previously reported under similar conditions.Recycling experiments also proved that the ribbons could be reused at least four times without obvious decay of decolorization rate and efficiency.This study suggests a tremendous application potential for amorphous ZVI in remediation of groundwater or wastewater contaminated with azo dyes. | Changqin Zhang Zhengwang Zhu Haifeng Zhang Zhuangqi Hu | 2012 | Journal of Environmental Sciences2012，24，6： | 5 |
| 8 | High He-ion irradiation resistance of CrMnFeCoNi high-entropy alloy revealed by comparison study with Ni and 304SS显示文摘High entropy alloys(HEAs) have presented potential applications in nuclear power plants owing to their novel atomic structure based high irradiation resistance. However, understanding of He-ion irradiation of HEAs is still lacking. In this work, we reveal He-ion irradiation resistance of HEA CrMnFeCoNi by comparison study with a pure Ni and a 304 stainless steel(304SS). It is found that the damage structure in the three materials can be characterized with He bubbles and stacking faults/stacking fault tetrahedrons((SFs/SFTs), which show a similar depth distribution after He-ion irradiation at both RT and 450℃.Although the He bubbles have a similar size about 2nm after irradiation at RT, the He bubble sizes of the HEA, 304SS, and Ni increase to 4.0±0.9,5.3±1.0 and 6.7 ±1.0 nm after irradiation at 450℃, respectively. Moreover, the density of SFs/SFTs displays in an order of Ni < 304 SS < HEA at both RT and 450℃.The He-ion irradiation at RT causes significant hardness enhancement for the three materials, however,compared to RT, after irradiation at 450℃, the Ni presents softening, while the 304SS, especially the HEA,shows further hardening. Thus, the HEA CrMnFeCoNi possesses the smallest He bubble size, the densest SFs/SFTs, and the highest hardening, indicating the best structural stability, as well as the best He-ion irradiation resistance, which can be attributed to its low mobility of He atoms and point defects. | Lixin Yang Hualong Ge Jian Zhang Ting Xiong Qianqian Jin Yangtao Zhou Xiaohong Shao Bo Zhang Zhengwang Zhu Shijian Zheng Xiuliang Ma | 2019 | Journal of Materials Science & Technology2019，35，3： | 5 |
| 9 | Census of Penguins on Ardley Island of Western Antarctic显示文摘 | ZHANG ZHENGWANG ZHENG GUANGMEI YUAN JUNFENG YANG WEIXIANG | 1994 | 生物多样性1994，2，B11： | 5 |
| 10 | The influence of Zr substitution for Nb on the corrosion behaviors of the Ni-Nb-Zr bulk metallic glasses显示文摘The influence of Zr content on corrosion behaviors of the Ni61.5Nb 38.5xZrx(x=1,3,5,7,9 at.%) bulk metallic glasses(BMGs) in 1 M HCl aqueous solution was investigated by potentiodynamic polarization measurements and X-ray photo-electron spectroscopy(XPS).It was found that these BMG alloys possess superior corrosion resistance,that is,with large passive region of about 1.5 V and low passive current density(as low as 0.05 Am-2 for Ni61.5Nb31.5Zr7).XPS analysis indicates that the high corrosion resistance is attributed to the formation of Nb-and Zr-enriched surface films formed in the aggressive acid solution.The Zr substitution for Nb effectively reduces the Ni content,particularly the metallic state Ni content in the surface films,which depresses the electrical conduction of the surface films and reduces the passive current density,thus leading to the enhancement of the corrosion resistance of these Ni-Nb-Zr BMGs.These alloys may potentially be useful for engineering applications. | LI DengKe ZHU ZhengWang ZHANG HaiFeng WANG AiMin HU ZhuangQi | 2012 | Science China(Physics,Mechanics & Astronomy)2012，55，12： | 5 |
| 11 | Effect of Sn addition on the glass-forming ability and mechanical properties of Ni-Nb-Zr bulk metallic glasses显示文摘The effect of tin (Sn) addition on the glass forming ability (GFA) and mechanical properties of the Ni-Nb-Zr ternary alloy system has been studied. The addition of Sn improves the GFA; Ni61Nb35.5-xZr3.5Snx (in at.%) alloys with x=1 can be cast into amorphous samples at least 3 mm in diameter using a copper mold injection-casting method. The critical size for glass formation decreases to 2 mm when x=5 because Ni2SnZr phase precipitates readily. The addition of Sn is also effective in enhancing the stability of the supercooled liquid; a maximum supercooled liquid region of 48 K was attained for the Ni61Nb30.5Zr3.5Sn5 alloy. Compression tests reveal that the Ni61Nb33.5Zr3.5Sn2 alloy possesses the best mechanical properties,with yield strength~3180 MPa,fracture strength~3390 MPa and plastic strain ~1.3%. The fracture surfaces examined by scanning electron microscopy indicate that the alloys have a transition from ductility to brittleness in fracture behavior. The combination of high GFA,high thermal stability,high strength and compressive plasticity makes these alloys potentially attractive for engineering applications. | LI DengKe ZHANG HaiFeng WANG AiMin ZHU ZhengWang HU ZhuangQi | 2011 | Chinese Science Bulletin2011，56，36： | 5 |
| 12 | Deformation Behavior of the Zr_(53.5)Cu_(26.5)Ni_5Al_(12)Ag_3 Bulk Metallic Glass Over a Wide Range of Strain Rate and Temperatures显示文摘The stress–strain relations for the Zr53.5Cu26.5Ni5Al12Ag3 bulk metallic glass(BMG) over a broad range of temperatures(room temperature to its supercooled liquid region) and strain rates(10-4to 10-1s-1) were established in uniaxial compression using a thermal-mechanical simulation system. The superplastic flow was seen above its glass transition temperature(Tg = 694 K) and strain rates of up to 10-1s-1from the variation of stress–strain curves. A deformation map of strain rate vs temperature of Zr53.5Cu26.5Ni5Al12Ag3 was obtained, which was mainly composed of homogeneous and inhomogeneous deformation regions,the former featuring either Newtonian or non-Newtonian flow while the latter characterizing linear elastic behavior followed by shear localization, respectively. A phenomenological constitutive equation used to describe a master curve of viscosity with respect to the strain rate was obtained by fitting the experimental results, which determines the viscosity of the present alloy at the temperature near and above Tg. The results show the Zr53.5Cu26.5Ni5Al12Ag3 BMG is the subject suitable for net shape forming process at the supercooled liquid region. | Guangcai Ma Zhengwang Zhu Zheng Wang Haifeng Zhang | 2015 | Journal of Materials Science & Technology2015，31，9： | 4 |
| 13 | Fe-based metallic glass:An efficient and energy-saving electrode material for electrocatalytic degradation of water contaminants显示文摘Excessive consumption of electrical energy has hampered the widespread application of electrochemical technology for degradation of various contaminants. In this paper, a Fe-based metallic glass(MG) was demonstrated as a new type of electrocatalyst to effectively and economically degrade an azo dye. In comparison to other typical electrodes, Fe-based MG electrodes exhibit a minimized degradation time, and the specific energy is 4-6 orders of magnitude lower than that of dimensionally stable anode(DSA), metal-like boron-doped diamond(BDD) and other electrodes. As sacrificial electrode materials, Fe-based MGs have less specific electrode mass consumption than iron electrodes. The use of Fe-based MGs will promote the practical application of electrochemical technology and the use of MGs as functional materials. | Xindong Qin Zhengkun Li Zhengwang Zhu Huameng Fu Hong Li Aimin Wang Hongwei Zhang Haifeng Zhang | 2018 | Journal of Materials Science & Technology2018，34，12： | 3 |
| 14 | Novel soybean peptide iglycin ameliorates insulin resistance of high-fat diet fed C57BL/6J mice and differentiated 3T3L1 adipocytes with improvement of insulin signaling and mitochondrial function显示文摘Soy consumption has been associated with potential health benefits in reducing chronic diseases.These physiological functions have been attributed to soy proteins or more commonly to bioactive peptides.Thus,more studies are required to identify these bioactive peptides,and elucidate their biological mechanisms of action.In the present study,a novel peptide iglycin was purifi ed from soybean seeds with a molecular mass of 3.88 k Da.Thereafter,iglycin reduced fasting blood glucose and restored insulin sensitivity of C57 BL/6 J mice on a high-fat diet with increased phosphorylation of insulin receptor substrate 1(IRS1)and AKT in adipose tissue.Furthermore,it improved glucose uptake,induced translocation of intracellular GLUT4 to plasma membrane and activation of insulin signaling in adipocytes under insulin-resistant condition.In addition,it decreased reactive oxygen species production,lipid peroxidation and inhibited adipocyte apoptosis with improved mitochondrial function as evidenced by up-regulation of succinate dehydrogenase activity,mitochondrial membrane potential and intracellular ATP store.These data suggested that iglycin ameliorated insulin resistance via activation of insulin signaling,which was associated with inhibition of oxidative stress,adipocyte apoptosis,and improvement of mitochondrial function. | Yinghuan Wu Ran Zhao Minxia Li Huiyun Li Zhengwang Chen Yanying Zhao | 2022 | Food Science and Human Wellness2022，11，6： | 3 |
| 15 | Highly efficient and stable CuZr-based metallic glassy catalysts for azo dye degradation显示文摘Metallic glasses with the unique disordered atomic structure and metastable nature have been recently applied to degrade the azo dyes and other organic pollutants based on their superior catalytic performance.In this work,the functional properties of six Cu Zr-based metallic glassy ribbons with the different nominal components in degrading Acid OrangeⅡ(AOⅡ)azo dyes were investigated.The Cu47.5Zr46Al6.5 metallic glassy ribbons could exhibit the more advanced catalytic performance for degradation process,which could completely degrade azo dye aqueous solution within 30 min.Additionally,the Cu47.5Zr46Al6.5 metallic glassy ribbons also showed the excellent cyclic stability along with approximately 97.68%degradation efficiency after 10 cycles.These excellent catalytic performance and stability are closely related to the synergistic effect of exposed copper nanoparticles and produced copper oxides in the reaction,which contributes to accelerate the generation of more hydroxyl radicals(·OH)to react with dye molecules.Our findings can be able to develop a novel potential metallic glassy material for the functional application of wastewater treatment. | Bowen Zhao Zhengwang Zhu Xin Dong Qin Zhengkun Li Haifeng Zhang | 2020 | Journal of Materials Science & Technology2020，42，11： | 3 |
| 16 | Molecular demographic history of the Hainan Peacock Pheasant(Polyplectron katsumatae) and its conservation implications显示文摘Knowledge of the historical responses of animal species to climate changes is critical for understanding their evolutionary history and conservation.During the late Quaternary period,Southeast Asia had a larger land area than today due to lower sea levels,and its terrestrial landscape was covered by extensive forests and savannah.To date,however,the general fluctuations in landscape distribution and their impacts on the demographics history of native species during the late Quaternary periods are still disputed.Specifically,the responses of animals on Hainan Island,which is located in the northernmost region of Southeast Asia,to historical climate changes,are poorly understood.Here,we performed a series of demographic analyses based on mitochondrial DNA genes to examine the response of the resident Hainan Peacock Pheasant(Polyplectron katsumatae) to climate change.Unlike the pattern of population collapse during the ice age and expansion during the warming period,we detected a historical expansion pattern in the demographic history of Hainan Peacock Pheasant through the late Quaternary period.It was concluded that the Hainan Peacock Pheasant survived through the late Quaternary periods,despite of its currently limited distribution and population size on Hainan Island.Anthropogenic influences must be considered in conservation planning due to their impacts on currently fragmented habitats and populations. | CHANG Jiang CHEN De LIANG Wei LI Ming ZHANG ZhengWang | 2013 | Chinese Science Bulletin2013，58，17： | 2 |
| 17 | Correlation Between Dynamic Compressive Strength and Fracture Behaviors of Bulk Metallic Glasses显示文摘The mechanical behaviors of Zr43.5Cu43.5Ni4Al8Nb1,Zr55.4Cu31.6Ni4Al8Nb1,Ti32.8Zr30.2Ni5.3Cu9Be22.7(at.%)metallic glass at different strain rates were studied.For all the present alloys,the dispersion over 700 MPa was observed on the strength in the repeated dynamic compressions,which was much stronger than that of the quasi-static compressive strength.Such the dispersion of the dynamic compressive strength was well correlated with the corresponding fracture behaviors.The area of fracture surface was calculated and also showed a strong dispersion for all the fractured specimens tested at the strain rate of 500 s^-1 and 1000 s^-1.All the specimens showed a linear relationship between the square of dynamic compressive strength and the area of fracture surface in the dynamic compression tests.This phenomenon was mainly thought to be related to the difference of mean initial free volume concentration of different samples,stress concentration caused by the split Hopkinson pressure bar experimental setup and high sensitivity of defects under dynamic deformation.These findings were beneficial to deeply understand the effect of strain rate on the mechanical properties of the metallic glass. | Wenqing Li Zhengwang Zhu Guojie Li Long Zhang Zhengkun Li Huameng Fu Hongwei Zhang Hong Li Aimin Wang Haifeng Zhang | 2020 | Acta Metallurgica Sinica(English Letters)2020，33，10： | 2 |
| 18 | Co_(78)Si_(8)B_(14)metallic glass:A highly efficient and ultra-sustainable Fenton-like catalyst in degrading wastewater under universal pH conditions显示文摘Overcoming the pH limitation and increasing the catalyst reusability remain pressing demands for metal-lic glass(MG)in wastewater remediation.Herein,Co_(78)Si_(8)B_(14)MG ribbons are used to degrade dye wastew-ater by activating hydrogen peroxide(H_(2)O_(2))as Fenton-like catalysts.The Co-based MG catalysts exhibit high degradation efficiency under both acidic and alkaline conditions,and the kinetic reaction rate at pH 10(0.176 min^(−1)) and pH 4(0.089 min^(−1)) is 5.9 and 1.2 times higher than that of the extensively studied Fe-based MG catalysts,respectively.Impressively,the Co-based MG catalysts can be reused up to 20-60 times at universal pH conditions,demonstrating fairly good reusability.The newly discovered Co-based MG catalysts do not follow the classical Fenton reactions with H_(2)O^(2) the way Fe-based MGs do.In an acid environment,hydroxyl radicals play a dominant role in the degradation,while in an alkaline environ-ment,the effect of hydroxyl radicals is weakened,and Co^(3+) ions exert a relatively major function on the degradation.The excellent performance in catalytic activity and reusability at universal pH conditions of the Co-based MGs will inspire the development of MGs in wastewater treatment. | Xindong Qin Jiliang Xu Zhengwang Zhu Zhengkun Li Dawei Fang Huameng Fu Shiming Zhang Haifeng Zhang | 2022 | Journal of Materials Science & Technology2022，，18： | 2 |
| 19 | Characterization of novel microsatellite markers of the Emei Shan Liocichla using restriction site-associated DNA sequencing显示文摘Background:The Emei Shan Liocichla(Liocichla omeiensis) is an endemic bird species to southwestern China with a small geographic range. However, little was known about the genetic status of this threatened species.Methods:We applied restriction-site-associated DNA sequencing(RAD-Seq) for rapid mass identification of microsatellite markers of the Emei Shan Liocichla.Results:A total of 11,564 microsatellite sequences were obtained, 600 random loci were designed for screening and 24 polymorphic microsatellite loci were selected for further validation. The average allele number, average observed heterozygosity and average expected heterozygosity were relatively low in our samples, which were 6.08, 0.6618 and 0.7048, respectively, indicating that the Emei Shan Liocichla might have lost some genetic diversity. Further analyses suggested that the populations distributed on two mountains(Daxiangling and Xiaoliangshan) showed a modest degree of genetic differentiation.Conclusions:These novel microsatellite markers provided valuable preliminary knowledge regarding the genetic status of the Emei Shan Liocichla and can be useful in further studies, as well as in the management and conservation of this species. | Ailin Yang De Chen Pengcheng Wang Yiqiang Fu Zhengwang Zhang | 2017 | Avian Research2017，8，2： | 2 |
| 20 | Research advances of Galliformes since 1990 and future prospects显示文摘Background: Galliformes are widely distributed throughout the world and economically important to humans as domesticated animals or gamebirds. They are at a unique position for advancing knowledge and techniques of wildlife conservation as the barometer of the status of applied ecology. Populations of many galliform species have declined mainly due to habitat loss and over-hunting. An assessment of knowledge of Galliformes could help to provide guidelines for future research and conservation strategies.Methods: Using the Web of Science search engine, we conducted a literature review of galliform-related articles published from 1990 to 2016. We used the 'research area' option to filter articles focused on the zoology, environmental sciences ecology, biodiversity conservation, forestry, behavioral sciences, reproductive biology, biochemistry and molecular biology, cell biology, genetics and heredity, evolutionary biology, physiology and developmental biology. We then checked duplication based on the title, abstract and full text. In addition, we examined the reference lists of selected studies to include the publications that were missed by above searching.Results: We retained 1874 articles related to the Galliformes from the initial 243,128 publications that were found. About 91.4% focused on one or two species, and 85.0% were conducted within a short duration, typically 1–2 years. The majority of the articles concentrated on macroscopic ecology(55.5%), mainly focusing on habitat selection or habitat use. With recent advances of molecular biology, the studies of taxonomy and phylogenetics rose quickly in last two decades. The study of physiology and biochemistry was no longer limited to simple description but expanded to the mechanisms of phenotype and micro-evolutionary potential. An additional area receiving increasing attention is the conservation of Galliformes, with the assessment of the conservation status and conservation management effectiveness of Galliformes(e.g. species diversity and genetic diversity) becoming the focus.Conclusions: The studies on Galliformes have made great achievements since 1990, but there are still gaps, particularly in macroscopic ecology, molecular genetics, and conservation. There is an urgent need to enhance long-term monitoring and analysis of population dynamics, and applying different disciplines to galliform conservation. Moreover, life history information of many galliform species is still lacking, which has hindered conservation efforts and effectiveness. In addition, multidiscipline studies and new technologies are not common for galliform studies, and should be encouraged. | Shan Tian Jiliang Xu Jianqiang Li Zhengwang Zhang Yong Wang | 2018 | Avian Research2018，9，3： | 2 |
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