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| 1 | Dynamic resistance exercise increases skeletal muscle-derived FSTL1 inducing cardiac angiogenesis via DIP2A-Smad2/3 in rats following myocardial infarction显示文摘Purpose:The aim of this study was to investigate the potential of dynamic resistance exercise to generate skeletal muscle-derived follistatin like-1(FSTL1),which may induce cardioprotection in rats following myocardial infarction(MI)by inducing angiogenesis.Methods:Male,adult Sprague-Dawley rats were randomly divided into 5 groups(n=12 in each group):sham group(S),sedentary MI group(MI),MI+resistance exercise group(MR),MI+adeno-associated virus(AAV)-FSTL1 injection group(MA),and MI+AAV-FSTL1 injection+resistance exercise group(MAR).The AAV-FSTL1 vector was prepared by molecular biology methods and injected into the anterior tibialis muscle.The MI model was established by ligation of the left anterior descending coronary artery.Rats in the MR and MAR groups underwent 4 weeks of dynamic resistance exercise training using a weighted climbing-up ladder.Heart function was evaluated by hemodynamic measures.Collagen volume fraction of myocardium was observed and analyzed by Masson’s staining.Human umbilical vein vessel endothelial cells culture and recombinant human FSTL1 protein or transforming growth factor-b receptor 1(TGFbR1)inhibitor treatment were used to elucidate the molecular signaling mechanism of FSTL1.Angiogenesis,cell proliferation,and disco interacting protein 2 homolog A(DIP2A)location were observed by immunofluorescence staining.The expression of FSTL1,DIP2A,and the activation of signaling pathways were detected by Western blotting.Angiogenesis of endothelial cells was observed by tubule experiment.One-way analysis of variance and Student’s t test were used for statistical analysis.Results:Resistance exercise stimulated the secretion of skeletal muscle FSTL1,which promoted myocardial angiogenesis,inhibited pathological remodeling,and protected cardiac function in MI rats.Exercise facilitated skeletal muscle FSTL1 to play a role in protecting the heart.Exogenous FSTL1 promoted the human umbilical vein vessel endothelial cells proliferation and up-regulated the expression of DIP2A,while TGFbR1 inhibitor intervention down-regulated the phosphorylation level of Smad2/3 and the expression of vascular endothelial growth factor-A,which was not conducive to angiogenesis.FSTL1 bound to the receptor,DIP2A,to regulate angiogenesis mainly through the Smad2/3 signaling pathway.FSTL1-DIP2A directly activated Smad2/3 and was not affected by TGFbR1.Conclusion:Dynamic resistance exercise stimulates the expression of skeletal muscle-derived FSTL1,which could supplement the insufficiency of cardiac FSTL1 and promote cardiac rehabilitation through the DIP2A-Smad2/3 signaling pathway in MI rats. | Yue Xi Meili Hao Qiaoqin Liang Yongxia Li Da-Wei Gong Zhenjun Tian | 2021 | Journal of Sport and Health Science2021，10，5： | 11 |
| 2 | Geochronology and Geochemical Characteristics of Intrusion in the Jinchangliang Gold Deposit,Inner Mongolia and Their Tectonic Significance显示文摘Discovered and mined in recent years, the Jinchangliang gold deposit has not yet been studied in its genetic type. In this paper, the geological features of ore deposit, S isotopic composition, metallogenic age and elements geochemical of the granite closely related to mineralization were discussed. The results of the geological features of ore deposit and S isotopic composition show that ore-bearing hydrothermal solution was closely related with the intrusion of magmatic. The granite is characteristic of high silica SiO_2=72.38%–72.98%, high aluminum and Al_2O_3=14.22%–14.35%, low calcium CaO=0.16% –0.26%, and low value of FeOT/MgO(6.86–7.73), and rich in alkalis Na_2O+K_2O=9.11%–9.24%, suggesting that it is high-K calc-alkaline, highly fractionated, weak aluminum A-type granite. The REE patterns are inclined to right and show intense fractionation between LREE and HREE, without obvious negative Eu anomaly(δEu=0.80–0.84). The primitive mantle-normalized spidergrams are characterized by depletion of Ba, U, Ta, Nb, Zr, Ti and P, which implies that the granite has the characteristics of the crust-mantle mixing. S isotopes also indicate that the material source of gold deposit is closely related to the granite rocks. The LA-ICP-MS Zircon UPb age of the Damiao rock mass medium-fine grained monzogranite(belonging to the early IndoChina) is(245±1) Ma. It shows that Jinchangliang gold deposit was not formed in Yanshanian, but the early Indo-China. Specifically speaking, the deposit was formed in the collision stage of the North China plate and the Siberian plate. | SUN Zhenjun SUN Guosheng YU Henan TIAN Yi LIU Shanli XIANG Zhu MENG Jian XUE Huaiyu LU Lina | 2015 | Acta Geologica Sinica(English Edition)2015，89，6： | 4 |
| 3 | The role of exercise in rehabilitation of discharged COVID-19 patients显示文摘Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)mainly caused pneumonia and pulmonary fibrosis through upper respiratory tract infection,which resulted in acute respiratory distress syndrome(ARDS)and multiorgan damage of cardiovascular,nervous,digestive,and genitourinary systems.Although the virus test turned negative after the patient recovered,the damage to multiorgan caused by SARS-CoV-2 may irreversible.Therefore,the health status of the recovered patients has gradually become the focus of people's attention.Whether coronavirus disease 2019(COVID-19)patients can receive exercise rehabilitation training after discharge?and what's the basis?We try to analyze and answer these questions,will provide some ideas about the patients to develop a reasonable and effective exercise rehabilitation program. | Wenyan Bo Yue Xi Zhenjun Tian | 2021 | Sports Medicine and Health Science2021，3，4： | 1 |
| 4 | Up-regulation of Thioredoxin 1 by aerobic exercise training attenuates endoplasmic reticulum stress and cardiomyocyte apoptosis following myocardial infarction显示文摘Exercise training(ET)has been reported to reduce oxidative stress and endoplasmic reticulum(ER)stress in the heart following myocardial infarction(MI).Thioredoxin 1(Trx1)plays a protective role in the infarcted heart.However,whether Trx1 regulates ER stress of the infarcted heart and participates in ET-induced cardiac protective effects are still not well known.In this work,H9c2 cells were treated with hydrogen peroxide(H_(2)O_(2))and recombinant human Trx1 protein(TXN),meanwhile,adult male C57B6L mice were used to establish the MI model,and subjected to a six-week aerobic exercise training(AET)with or without the injection of Trx1 inhibitor,PX-12.Results showed that H_(2)O_(2)significantly increased reactive oxygen species(ROS)level and the expression of TXNIP,CHOP and cleaved caspase12,induced cell apoptosis;TXN intervention reduced ROS level and the expression of CHOP and cleaved caspase12,and inhibited cell apoptosis in H_(2)O_(2)-treated H9c2 cells.Furthermore,AET up-regulated endogenous Trx1 protein expression and down-regulated TXNIP expression,restored ROS level and the expression of ER stress-related proteins,inhibited cell apoptosis as well as improved cardiac fibrosis and heart function in mice after MI.PX-12 partly inhibited the AET-induced beneficial effects in the infarcted heart.This study demonstrates that Trx1 attenuates ER stress-induced cell apoptosis,and AET reduces MI-induced ROS overproduction,ER stress and cell apoptosis partly through up-regulating of Trx1 expression in mice with MI. | Mengxin Cai Zujie Xu Wenyan Bo Fangnan Wu Wenpu Qi Zhenjun Tian | 2020 | Sports Medicine and Health Science2020，2，3： | 1 |
| 5 | Resistance Exercise Increases the Regulation of Skeletal Muscle FSTL1 Consequently Improving Cardiac Angiogenesis in Rats with Myocardial Infarctions显示文摘Purpose The aim of this study was to investigate whether skeletal muscle-derived follistatin-like 1(FSTL1)reaches the heart and exerts the angiogenetic function in rats suffering myocardial infarctions(MI)after exercise intervention.Methods Forty-eight male adult Sprague-Dawley rats were randomly divided into four groups.MI was provoked by ligation of left anterior descending coronary artery.MI rats underwent adeno-associated virus injection of FST1 in tibialis anterior muscle and 4 weeks of resistance exercise via a tail-suspended incremental weight-climbing method(0-75%body weight,daily load increased by 10%;1 h/day,5 day/w).Heart function was evaluated by hemodynamics including LVSP,LVEDP and±dP/dt max;the cross-sectional area of muscle cells and myocardium fibrosis were analyzed by DiI and Masson’s stain-ing,respectively;the FSTL1 expression,endothelial cell proliferation and angiogenesis were visualized by immunofluores-cence staining;and protein expression was quantified by Western blotting.Results Resistance exercise reverted MI-induced skeletal muscle atrophy,increased muscle FSTL1 expression and stimulated skeletal muscle derived FSTL1 entering into the MI heart via blood circulation.The overexpression of skeletal muscle FSTL1 improved myocardial endothelial cell proliferation,increased small vessel density in the fibrotic border,inhibited myocardial fibrosis and improved heart function in the MI rats after the exercise intervention.Meanwhile,DIP2A-PI3K-Akt-mTOR,Erk1/2 and TGFβ-Smad2/3 pathways were activated in the myocardium.Conclusion Resistance exercise stimulates skeletal muscle derived FSTL1 to reach the myocardium which makes a positive contribution to cardioprotection in MI rat. | Yue Xi Meili Hao Zhenjun Tian | 2019 | Journal of Science in Sport and Exercise2019，1，1： | 0 |
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