|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Cloning and expression of MXR7 gene in human HCC tissue显示文摘AIM To clone and identify the whole cDNA ofMXR7 gene and to find out its expression inhuman HCC,and normal tissues.METHODS The DNA primers were designed andsynthesized according to the whole cDNAsequence of MXR? gene.The cDNA of humanHCC was taken as the template while the cDNA ofMXR7 gene was synthesized by polymerasechain reaction(PCR).Recombinant DNAconforming to reading frame was constructed byconnecting purified PCR product of the cDNA ofMXR? gene with expression vector pGEX-5X-1 offusion protein.The plasmid MXRT/pGEX-5X-1was identified by sequencing.Using 32p labeledMXR? cDNA as probe,MXR7 mRNA expressionwas detected by Northern blot analysis in 12different human normal tissues,7 preoperativelyuntreated non-liver tumor tissues,30preoperatively untreated HCC,theparacancerous liver tissues and 12 normal livertissues samples.RESULTS Restriction enzyme and sequenceanalysis confirmed that the insertion sequence invector pGEX-5X-1 was the same as the cDNAsequence of MXR7 gene.Northern blot analysisshowed no expression of MXR? mRNA in 12 kindsof normal human tissues including liver,7 tumortissues in other sites and 12 normal livertissues,the frequencies of MXR7 mRNA expression in HCC and paracancerous livertissues were 76.6% and 13.3%,respectively.The frequency of MXR7 mRNA expression in HCCwithout elevation of serum AFP and in HCC<5cm was 90%(9/10)and 83.3%(5/6),respectively.CONCLUSION MXR7 mRNA is highly expressedin human HCC,which is specific and occurs at anearly stage of HCC,suggesting MXR7 mRNA canbe a tumor biomarker for HCC.The detection ofMXR7 mRNA expression in the biopsied livertissue is helpful in discovering early subclinicalliver cancer in those with negative serum AFP. | Zhou XP Wang HY Yang GS Chen ZJ Li BA Wu MC | 2000 | World Journal of Gastroenterology2000，6，1： | 23 |
| 2 | Up-regulation of PIK3CA promotes metastasis in gastric carcinoma显示文摘AIM:To explore expressions of PIK3CA in the progression of gastric cancer from primary to metastasis and its effects on activation of phosphatidylinositol 3-kinase(PI3K)/Akt pathway.METHODS:mRNA and protein levels of PIK3CA were assessed,respectively,by real-time quantitative polymerase chain reaction and immunohistochemistry in specimens of normal gastric mucosa,primary foci and lymph node and distant metastasis of gastric cancer.Akt and phosphorylated Akt protein were also examined by Western blotting in these tissues,in order to analyze the effect of PIK3CA expression level changes on the activation of PI3K/Akt signaling pathway.RESULTS:PIK3CA mRNA in lymph node metastasis were approximately 5 and 2 folds higher,respectively,than that in the corresponding normal gastric mucosa and primary gastric cancer tissues(P<0.05),while no statistical significance was found compared with distant metastasis.Immunohistochemically,PIK3CA protein expression was discovered in 7(35%)specimens of 20 primary foci vs 10(67%)of 15 of lymph node metastasis or 11(61%)of 18 of distant metastasis(35%vs 67%,P=0.015;35%vs 61%,P=0.044).With the increased level of PIK3CA expression,the total Akt protein expression remained almost unchanged,but p-Akt protein was upregulated markedly.CONCLUSION:Increased expression of PIK3CA is expected to be a promising indicator of metastasis in gastric cancer.Up-regulation of PIK3CA may promote the metastasis of gastric cancer through aberrant activation of PI3K/Akt signaling. | Liu JF Zhou XK Chen JH Yi G Chen HG Ba MC Lin SQ Qi YC. | 2010 | World Journal of Gastroenterology2010，16，39： | 20 |
| 3 | 利钠肽受体2的缺乏促进小鼠二叶主动脉瓣、主动脉瓣疾病、左心室功能不全以及升主动脉扩张显示文摘主动脉瓣疾病是无有效药物治疗的细胞介导的病变过程。C型利钠肽(C-type natriuretic peptide,CNP)抑制瓣膜间质细胞(valve interstitial cells,VIC)的肌纤维形成和成骨过程,并且在出现主动脉瓣狭窄时水平下调。然而,CNP信号是否调节体内主动脉瓣的健康状态尚不清楚。该研究旨在确定小鼠CNP信号通路的缺失是否导致主动脉瓣疾病加速发展。 | 刘青 叶鹏 Blaser MC Wei K Adams RL Zhou YQ Caruso LL Mirzaei Z Lam A Tam RK Zhang H Heximer SP Henkelman M Simmons CA | 2018 | 中华高血压杂志2018，26，1： | 3 |
| 4 | TNFRl-induced NF-kappaB, but not ERK,p38MAPK or JNK activation,mediates TNF-in- duced ICAM-1 and VCAM-1 expression on endothelial cells 显示文摘 | Zhou Z Connell MC MacEwan DJ | 2007 | Cell Signal2007，19，6： | 1 |
| 5 | TNFR1 induced NF-kappaB,but not ERK, p38 MAPK or JNK activation, me- diates TNF induced ICAM 1 and VCAM 1 expression on endothelial cells显示文摘 | Zhou Z Connell MC MacEwan DJ | 2007 | Cell Signal2007，19，6： | 1 |
| 6 | Wnt,hedgehog and snail:sister pathways that control by GSK-3h and h-Trcp in the regulation of metastasis显示文摘 | Zhou BP Hung MC | 2005 | Cell Cycle2005，4，： | 1 |
| 7 | Long non - coding RNA MALAT -1overexpression predicts tumor recurrence of hepatocellular carcinomaafter liver transplantation 显示文摘 | Lai MC Yang Z Zhou L | 2012 | Med Oncol2012，29，3： | 1 |
| 8 | Oestrogen replacement therapy promotes hone healing around dental implants in osteoporotic rats显示文摘 | Qi MC Zhou XQ Hu J | 2004 | Int J Oral Maxillofac Surg2004，33，3： | 1 |
| 9 | The investigational aurora kinase a inhibitor MLN8237 induces defects in cell viability and cell-cy- cle progression in malignant bladder cancer cells in vitro and in viva显示文摘 | Zhou N Singh K Mir MC | 2013 | Cli Cancer Res2013，19，7： | 1 |
| 10 | Theorphan nuclear receptor ROR gammat directs thedifferentiation program of proinfl ammatory IL-17+T helper cells显示文摘 | Ivanov I I Mc Kenzie B S Zhou L | 2006 | Cell2006，126，25： | 1 |
| 11 | TNFR1-induced NF-kappaB, but not ERK, p38MAPK or JNK activation, mediates TNF-induced ICAM-1 and VCAM-1 expression on endothelial cells显示文摘 | Zhou Z Connell MC MacEwan DJ | 2007 | Cell Signal2007，19，6： | 1 |
| 12 | Vascular endothelial growth factor ligands and receptors that regulate human cytotrophoblast survival are dysregulated in severe preeclampsia and hemolysis,elevated liver enzymes,and low platelets syndrome显示文摘 | Zhou Y Mc Master M Woo K | 2002 | Am J Pathol2002，160，4： | 1 |
| 13 | HER-2/neu blocks tumor necrosis factor-induced apoptosis via the Akt/NF-kappaB pathway显示文摘 | Zhou BP Hu MC Miller SA | 2000 | J Biol Chem2000，275，： | 1 |
| 14 | Executive decline and dysfunction precedes declines in memory: the Women's Health and Aging Study II 显示文摘 | Carlson MC XUE QL ZHOU J | 2009 | J Gerontol A Biol Sci Med Sci2009，64，1： | 1 |
| 15 | Compensatory remodeling insymptomatic middle cerebral artery atherosclerotic stenosis : a highresolutionMRI and microemboli monitoring study 显示文摘 | Shi MC Wang SC Zhou HW | 2012 | NeurologicalResearch2012，34，2： | 1 |
| 16 | O estrogen replacement therapy promotes bone healing around dental implants in osteoporotic rats显示文摘 | Qi MC Zhou XQ Hu J | 2004 | Int J Oral Maxilofac Surg2004，33，3： | 1 |
| 17 | Long non-coding RNA MALAT-1 overexpression predicts tumor recurrence of hepatocellular carcino- ma after liver transplantation显示文摘 | Lai MC Yang Z Zhou L | 2012 | Med Oncol2012，29，3： | 1 |
| 18 | Vascular endothelial growth fac- tor ligands and receptors that regulate human cytotrophoblast survival are dysregulated in severe preeclampsia and hemolysis, elevated liver enzymes,and low platelets syndrome 显示文摘 | Zhou Y Mc Master M Woo K | 2002 | Am J Pathol2002，160，4： | 1 |
| 19 | Role of platelet activating factor in panaeaatifis associated acute lung injury in the rat显示文摘 | Zhou WG Mc Collum MO Levine BA | 1992 | Am J Pathol1992，140，： | 1 |
| 20 | Oestrogen replacement thera- py promotes bone healing around dental implants in osteoporotic rats显示文摘 | Qi MC Zhou XQ Hu J | 2004 | Int J Oral Maxillofac Surg2004，33，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员