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| 1 | Ex vivo 3D osteocyte network construction with primary murine bone cells显示文摘Osteocytes reside as three-dimensionally(3D) networked cells in the lacunocanalicular structure of bones and regulate bone and mineral homeostasis. Despite of their important regulatory roles, in vitro studies of osteocytes have been challenging because:(1) current cell lines do not sufficiently represent the phenotypic features of mature osteocytes and(2) primary cells rapidly differentiate to osteoblasts upon isolation. In this study, we used a 3D perfusion culture approach to:(1) construct the 3D cellular network of primary murine osteocytes by biomimetic assembly with microbeads and(2) reproduce ex vivo the phenotype of primary murine osteocytes, for the first time to our best knowledge. In order to enable 3D construction with a sufficient number of viable cells, we used a proliferated osteoblastic population of healthy cells outgrown from digested bone chips. The diameter of microbeads was controlled to:(1) distribute and entrap cells within the interstitial spaces between the microbeads and(2) maintain average cell-to-cell distance to be about 19 mm. The entrapped cells formed a 3D cellular network by extending and connecting their processes through openings between the microbeads. Also, with increasing culture time, the entrapped cells exhibited the characteristic gene expressions(SOST and FGF23) and nonproliferative behavior of mature osteocytes. In contrast, 2D-cultured cells continued their osteoblastic differentiation and proliferation. This 3D biomimetic approach is expected to provide a new means of:(1) studying flow-induced shear stress on the mechanotransduction function of primary osteocytes,(2) studying physiological functions of 3D-networked osteocytes with in vitro convenience,and(3) developing clinically relevant human bone disease models. | Qiaoling Sun Yexin Gu Wenting Zhang Leah Dziopa Jenny Zilberberg Woo Lee | 2015 | Bone Research2015，3，3： | 2 |
| 2 | Anemia,transfusions and hospital outcomes among critically ill patients on prolonged acute mechanical ventilation:a retrospective cohort study显示文摘 | Zilberberg MD Stern LS Wiederkehr DP | 2008 | Critical Care2008，12，2： | 1 |
| 3 | Ventilator-associated pneumonia: the clinical pulmonary infection score as a surrogate for diagnostics and outcome显示文摘 | Zilberberg MD Shorr AF | 2010 | Clin Infect Dis2010，51，1： | 1 |
| 4 | Diagnostics and epidemiology in ventilator-associated pneumonia显示文摘 | Shorr AF Chan CM Zilberberg MD | 2011 | Ther Adv Respir Dis2011，5，2： | 1 |
| 5 | Acute lung injury in the medical ICU : comorbid conditions, age, etiology, and hospital outcome显示文摘 | Zilberberg M D Epstein S K | 1998 | Am J Rrspir Crit Care Med1998，157，： | 1 |
| 6 | Oxygen adsorption on the Ag(lll) surface: a quantum chemical study by the NDDO/MC method 显示文摘 | MILOV M A ZILBERBERG I L RUZANKIN S PH | 2000 | Journal of Molecular Catalysis A: Chemical2000，158，1： | 1 |
| 7 | Inverted organic solar cells with sol-gel processed high work-function vanadium oxide hole-extraction layers显示文摘 | Zilberberg K Trost S Meyer J | | 0，，24： | 1 |
| 8 | Latent TGF-β-binding proteins显示文摘 | Robertson IB Horiguchi M Zilberberg L | 2015 | Matrix Biol2015，8，5： | 1 |
| 9 | Do poor-responder patients benefit from increasing the daily gonadotropin dose during controlled ovarian hyperstimulation for IVF?显示文摘 | Haas J Zilberberg E Machtinger R | 2015 | Gynecol Endocrinol2015，31，： | 1 |
| 10 | p38 MAPK is an early determinant of promiscuous Smad2/3 signaling in the aortas of fibrillin-1(Fbn1)null-mice显示文摘 | CARTA L SMALDONE S ZILBERBERG L | 2009 | J Biol Chem2009，284，9： | 1 |
| 11 | A rapid and sensitive bioassay to measure bone morphogenetic protein activity 显示文摘 | Zilberberg L Dijke P Sakai L Y | 2007 | BMC Cell Biol2007，8，： | 1 |
| 12 | KCNK2: reversible conversion of a hippocampal potassium into a voltagedependent channel显示文摘 | Bockenhauer D Zilberberg N Goldstein SA | 2001 | Nat Neurosci2001，4，5： | 1 |
| 13 | Diagnostics and epidemiology in ventilator - associated pneumonia 显示文摘 | SHORR A F CHAN C M ZILBERBERG M D | 2011 | Ther Adv Respir Dis2011，5，2： | 1 |
| 14 | Anemia, transfusions and hospital outcomes among critically ill patients on prolonged acute mechanicalventilation: a retrospective cohort study显示文摘 | Zilberberg MD Stern LS Wiederkehr DP | 2008 | Crit Care2008，12，： | 1 |
| 15 | Genetic pol- ymorphism and expression of a highly potent scorpion depress- ant toxin enable refinement of the effects on insect Na channels and illuminate the key role of Asn-58显示文摘 | Strugatsky D Zilberberg N Stankiewcz M | 2005 | Biochemistry2005，44，25： | 1 |
| 16 | An 'old word' scorpi- on beta-toxin that recognizes both insect and mammalian sodi- um channels显示文摘 | Gordom D Ilan N Zilberberg N | 2003 | European Journal of Biochemistry2003，270，12： | 1 |
| 17 | Economic aspects of prevent- ing health care-associated infections in the intensive care unit 显示文摘 | Zilberberg MD Shorr AF | 2012 | Crit Care Clin2012，28，1： | 1 |
| 18 | Ventilator-associated pneumonia:the clinical pulmonary infection score as a surrogate for diagnostics and outcome显示文摘 | Zilberberg M D Shorr A F | 2010 | Clin Infect Dis2010，51，： | 1 |
| 19 | Acute lung injury in the medical ICU:comorbid conditions, age, etiology, and hospital outcome 显示文摘 | Zilberberg MD Epstein SK | 1998 | Am J Respir Crit Care Med1998，157，： | 1 |
| 20 | Appropriate Antibiotic Treatment in Severe Sepsis and Septic Shock: Timing Is Everything显示文摘 | ZILBERBERG MD SHORR AF | 2015 | Crit Care Med2015，43，10： | 1 |
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