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| 1 | The CD40/CD154 receptor/ligand dyadRID='†'ID='†' Review显示文摘 | U. Sch?nbeck*RID='*'ID='*' Corresponding author. P. Libby | 2001 | Cellular and Molecular Life Sciences2001，，1： | 1 |
| 2 | The CD40/CD154 receptor/ligand dyadRID='†'ID='†' Review显示文摘 | U. Sch?nbeck*RID='*'ID='*' Corresponding author. P. Libby | 2001 | Cellular and Molecular Life Sciences2001，，1： | 1 |
| 3 | Bioaccumulation of Au(Ⅲ) by cyanobacteria Anabaena azollae显示文摘The gold accumulation efficiency of culturing Anbaena azollae (a filamentous speciees of cyanobacteria in gold bearing medium is observed. It is indicated that it is possible to produce metal combined proteins by culturing metal tolerating cyanobacteria ain metal bearing solution. | ZHAO Jizhen 1, SHI Dingji 2 and ZHANG Yun 1* 1. Life Science College, Peking University, Beijing 100871, China 2. Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China. * Corresponding author. | 1998 | Chinese Science Bulletin1998，43，13： | 0 |
| 4 | Influence of site-directed mutation of cytochrome b_5 Phe35 on the protein's structure and properties显示文摘Kinetic studies of the heme dissociation from the wild type and Phe35Tyr, Phe35Leu mutants of bovine liver microsomal ferricytochrome b 5 indicate that the oxidized Phe35Tyr mutant is more stable towards denaturant than wild type but Phe35Leu mutant proceeded with a different mechanism compared with wild type cytochrome b 5 and Phe35Tyr mutant protein. Because of the decrease of side chain volume in Phe35Leu mutant, a cavity produced in the interior of the protein may offer a channel for urea molecule to enter the hydrophobic pocket. When urea concentration is larger than 5 mol/L, the urea molecule may compete to coordinate the iron of heme with His39, that results in sharp increase of the rate of heme dissociation. The interaction between cytochrome b 5 and cytochrom c demonstrated that a 1:1 protein complex was formed between the two proteins. The binding constants of cytochrome b 5 with cytochrome c are: wild type K A=4.2(±0.01)×10 6(mol/L) -1 , Phe35Tyr K A=3.7(±0.01)×10 6(mol/L) -1 and Phe35Leu K A=4.7(±0.01)×10 6(mol/L) -1 respectively ( I =1 m mol/L, pH 7.0 soldium phosphate buffer, 25℃). These results clearly show that the mutation at Phe35 has no influence on the binding of cytochrome b 5 with cytochrome c and that the hydrophilic patch residues are not involved in the binding of cytochrome b 5 and cytochrome c. | YAO Ping 1, WANG Yunhua 1, SUN Yulong 1, XIE Yi 2 and HUANG Zhongxian 1* 1. Department of Chemistry, Fudan University, Shanghai 200433, China 2. Institute of Genetics, Fudan University, Shanghai 200433, China. *Corresponding author. | 1998 | Chinese Science Bulletin1998，43，3： | 0 |
| 5 | Research advances in gene therapy for Parkinson's disease显示文摘During the long period of time when people have been seeking for an effective therapeutic method for PD, the gene therapy has shown greater and greater advantages over other methods. It can be performed mainly in two ways: ex vivo and in vivo. With the former, TH gene as well as some neurotrophic factor genes (such as GDNF, BNDF genes) can be invited to some cell lines or primary cells thus forming engineered cells and then implanting them into brain. While with the latter, viral vectors including HSV-1, Ad, AAV that can be utilized to construct recombinant viruses, or non-virus vectors can be used to delived DNA into brain directly. The present review summarizes the recent research advances in the gene therapy for PD, and it is reasonable for us to predict a notable progress in prevention and treatment for PD in the next decade. | MA Duanduan, WANG Xiaomin * and HAN Jisheng Neuroscience Research Institute, Beijing Medical University, Beijing 100083, China. * Corresponding author. | 1998 | Chinese Science Bulletin1998，43，5： | 0 |
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