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| 1 | Mesencephalic astrocyte-derived neurotrophic factor ameliorates steatosis in HepG2 cells by regulating hepatic lipid metabolism显示文摘BACKGROUND Nonalcoholic fatty liver disease (NAFLD) is a global metabolism-associated liver disease.Mesencephalic astrocyte-derived neurotrophic factor (MANF) is a newly discovered secreted protein that is involved in metabolic homeostasis.However,much remains to be discovered about its function in hepatic lipid metabolism;thus,we assessed whether MANF could regulate hepatic metabolism.AIM To establish in vivo and in vitro NAFLD models to explore the role of MANF in hepatic lipid metabolism.METHODS HepG2 cells treated with free fatty acids (FFAs) and ob/ob mice were used as NAFLD models.Liver tissues collected from wild type and ob/ob mice were used to detect MANF expression.Cells were treated with FFAs for different durations.Moreover,we used lentiviral constructs to establish overexpression and knockdown cell models in order to interfere with MANF expression levels and observe whether MANF influences hepatic steatosis.Western blot analysis and quantitative real-time PCR were used to detect protein and gene expression,and oil red O staining was used to visualize intracellular lipid droplets.RESULTS Hepatic MANF protein and mRNA expression in wild type mice were 10-fold and 2-fold higher,respectively,than those in ob/ob mice.The MANF protein was temporarily increased by 1.3-fold after stimulation with FFAs for 24 h and gradually decreased to 0.66-fold that of the control at the 72 h time point in HepG2 cells.MANF deficiency upregulated the expression of genes involved infatty acid synthesis,cholesterol synthesis,and fatty acid uptake and aggravated HepG2 cell steatosis,while MANF overexpression inhibited fatty acid synthesis and uptake and cholesterol synthesis,and rescued HepG2 cells from FFAsinduced steatosis.Furthermore,a significant decrease in triglyceride levels was observed in the MANF overexpression group compared with the control group(0.4288±0.0081 mmol/g vs 0.3746±0.0121 mmol/g,P <0.05) upon FF As treatment.There was also a 17%decrease in intracellular total cholesterol levels between the MANF overexpression group and the control group (0.1301±0.0059mmol/g vs 0.1088±0.0009 mmol/g,P <0.05) upon FF As treatment.Moreover,MANF suppressed lipid deposition in HepG2 cells.CONCLUSION Our findings indicate that MANF improves the phenotype of liver cell steatosis and may be a potential therapeutic target in hepatic steatosis processes. | Miao He Cong Wang Xiao-Hong Long Jia-Jia Peng Dong-Fang Liu Gang-Yi Yang Michael D Jensen Li-Li Zhang | 2020 | World Journal of Gastroenterology2020，26，10： | 3 |
| 2 | Cooperation in an asymmetric volunteer's dilemma game with relatedness显示文摘What motivates some members of a social group to voluntarily incur costs in order to provide for the common good? This question lies at the heart of theoretical and empirical studies of cooperative behavior. This is also the question that underlies the classic volunteer's dilemma model, which has been previously explored in scenarios where group members are related or interact asym- metrically. Here we present a model that combines asymmetry and relatedness, showing that the probability of volunteerism in such systems depends closely on both the degree of asymmetry and level of relatedness between interacting individuals. As has been shown in previous volunteer's dilemma models, the payoff ratio and overall group size also influence the probability of volunteerism. The probability of volunteerism decreases with increasing group size or decreasing cost-to-benefit ratio of the coplayers; in the presence of asymmetrical interactions, subordinate players were more likely to offer public goods than the dominant player. More asymmetrical interactions decrease the probability of volunteerism of the dominant player; overall volunteerism increases with increasing relatedness. | HE JunZhou WANG RuiWu CHRISTOPHER X J Jensen LI YaoTang LI ChaoQian | 2012 | Chinese Science Bulletin2012，57，16： | 2 |
| 3 | A non-traumatic Staphylococcus aureus osteomyelitis model in pigs显示文摘 | Jensen HE Nielsen OL Agerholm JS | 2010 | In Vivo2010，24，3： | 1 |
| 4 | Mitogen-activated protein kinase-defective Candida albicans is avirulent in a novel model of localized murine candidiasis 显示文摘 | Guhad FA Jensen HE Aalbaek B | 1998 | FEMS Microbiol Lett1998，166，1： | 1 |
| 5 | Increased urinary albumin excretion in begin essential hypertension 显示文摘 | Parring HH Jensen HE Mogensen CE | 1994 | Lancet1994，1，： | 1 |
| 6 | Characterization of human soluble high and low activity catechol-O- methyltransferase catalyzed catechoI estrogen methylation 显示文摘 | Goodman JE Jensen LT He P | 2002 | Pharmacogenetics2002，12，7： | 1 |
| 7 | Canopy-air temperature of crops grown under different irrigation regimes in a temperatehumid climate 显示文摘 | Jensen HE Svendsen H Jensen SE | 1990 | Irrigation Sci1990，11，： | 1 |
| 8 | Inland wetland change detection using aircraft MSS data 显示文摘 | Jensen J R Ramsey E W Mackey Jr HE | 1987 | Photogram metric Engineering & Remote Sensing1987，53，5： | 1 |
| 9 | Increased serum erythropoietin level during azathioprine treatment in renal transplant recipients显示文摘 | Jensen JD Hansen HE Pedersen EB | | 0，，： | 1 |
| 10 | Approaches and recent development of polymer electrolyte membranes for fuel cells operating above 100℃ 显示文摘 | LI Q HE R JENSEN J O | 2003 | Chem Mater2003，15，： | 1 |
| 11 | Comparison of the angiotensin Ⅱantagonist losartan with the angiotensin converting enzyme inhibitor enapril in patients with essential hypertension显示文摘 | Tikkanen I Omvik P Jensen HE | 1995 | J Hypertens1995，13，： | 1 |
| 12 | The CD19 compartment in myeloma includes a population of clonal cells persistent after high-dose treatment显示文摘 | Rasmussen T Jensen L Johnsen HE | 2002 | Leuk Lymphoma2002，43，5： | 1 |
| 13 | PBI-based polymer membranes for high temperature fuel cells-preparation,characterization and fuel cell demonstration显示文摘 | Li Q F He R H Jensen J O | 2004 | Fuel Cell2004，4，3： | 1 |
| 14 | Approaches and recent development of polymer electrolyte membranes for fuel cells operating above 100oC 显示文摘 | Li Q He R Jensen JQ | 2003 | Chem Mater2003，15，： | 1 |
| 15 | An enzyme-based in situ hybridisation method for the identification of Streptococcus suis显示文摘 | Madsen LW Boye M Jensen HE | 2001 | APMIS2001，109，10： | 1 |
| 16 | Approaches and recent development of polymer electrolyte membranes for fuel cells operating above 100 ℃显示文摘 | Li Q He R Jensen J Q | | 0，，： | 1 |
| 17 | Approaches and recent develop- ment of polymer electrolyte membranes for fuel ceils operating above 100 ℃ 显示文摘 | LI Q F HE R H JENSEN J | 2003 | Chemistry of Materials2003，15，： | 1 |
| 18 | Approaches and recent development of polymer electrolyte membranes for fuel cells operational above 100 ℃ 显示文摘 | Li Q F He R H Jensen J Q | 2003 | Chem Ma- ter2003，15，： | 1 |
| 19 | Canopy-air temperature of crops grown underdifferent irrigation regimes in a temperature humid climate显示文摘 | JENSEN HE SVENDSEN H JENSEN SE | 1990 | Irrigation Science1990，11，： | 1 |
| 20 | Dieulafoys Lesion 显示文摘 | Hoffman J Beck H Jensen HE | 1984 | Surg Gynecol Obstet1984，159，4： | 1 |
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