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| 1 | Suppressing tawny crazy ant (Nylanderia fulva) by RNAi technology显示文摘A bstract The tawny crazy ant(Nylanderia fulva)is a new invasive pest in the United States.At present,its management mainly relies on the use of synthetic insecticides,which are generally ineffective at producing lasting control of the pest,necessitating alternative environmentally friendly measures.In this study,we evaluated the feasibility of gene silencing to control this ant species.Six housekeeping genes encoding actin(NfActin),coatomer subunit β (NfCOPP),arginine kinase(NfArgK),and V-type proton ATPase subunits A(NfvATPaseA),B(NfvATPaseB)and E(NfvATPaseE)were cloned.Phylogenetic analysis revealed high sequence similarity to homologs from other ant species,particularly the Florida carpenter ant(Camponotus floridanus).To silence these genes,vector L4440 was used to generate six specific RNAi constructs for bacterial expression.Heat-inactivated,dsRNA-expressing Escherichia coli were incorporated into artificial diet.Worker ants exhibited reduced endogenous gene expression after feeding on such diet for 9 d.However,only ingestion of dsRNAs of NfCOPfi(a gene involved in protein trafficking)and NfArgK(a cellular energy reserve regulatory gene in invertebrates)caused modest but significantly higher ant mortality than the control.These results suggest that bacterially expressed dsRNA can be orally delivered to ant cells as a mean to target its vulnerabilities.Improved efficacy is necessary for the RNAi-based approach to be useful in tawny crazy ant management. | Jia Meng Jiaxin Lei Andrew Davitt Jocelyn RHolt Jian Huang Roger Gold Edward L Vargo Aaron MTarone Keyan Zhu-Salzman | 2020 | Insect Science2020，27，1： | 2 |
| 2 | Open Reduction and Rigid Internal Fixation of Mandibular Condylar Fractures by an Intraoral Approach:A Long-Term Follow-Up Study of 15 Patients显示文摘 | Thomas Jensen John Jensen Sven Erik N rholt Martin Dahl Lone Lenk-Hansen Peter Svensson | | 0，，： | 1 |
| 3 | An extended LeFort Ⅰ osteotomyfor correction of midface hypoplasia 显示文摘 | Nφrholt SE Sindet-Pedersen S Jensen AJ | 1996 | J Oral Maxillofac Surg1996，54，： | 1 |
| 4 | 水幕——一项防止高压不衬砌岩石洞室气体渗漏的成功技术显示文摘1 引言 迄今为止,尚未能在坚硬岩体中建成专门贮存天然气或用作压缩气体能量贮存装置的洞室,尽管人们已经做了大量工作,以确立这种贮气装置的经济和技术的基础。 | H.KjΦrholt E.Broch 顾圣平 | 1994 | 河海科技进展1994，14，4： | 1 |
| 5 | Water Curtain-A Successful Means of Preventing Gas Leakage from HighPressure,Unlined Rock Caverns显示文摘 | Kjфrholt H Broch E | 1992 | Tunneling and Underground Space Technology1992，，2： | 1 |
| 6 | Air Cushion Surge Chamber for Underground Power Plants显示文摘 | Goodall D C Kjфrholt H Tekle T | 1988 | Water Power & Dam Construction1988，，11： | 1 |
| 7 | The effects of trans-resveratrol on insulin resistance,inflammation,and microbiota in men with the metabolic syndrome:A pilot randomized,placebo-controlled clinical trial显示文摘Background and Aim:The metabolic syndrome(MetS)is a pathological condition comprised of abdominal obesity,insulin resistance,hypertension,and hyperlipidemia.It has become a major threat globally,resulting in rapidly increasing rates of diabetes,coronary heart disease,and stroke.The polyphenol resveratrol(RES)is believed to improve glucose homeostasis and insulin resistance by activating sirtuin,which acetylates and coactivates downstream targets and affects glucose and lipid homeostasis in the liver,insulin secretion in the pancreas,and glucose uptake in skeletal muscle.We studied the effects of RES on insulin resistance,glucose homeostasis,and concomitant effects on adipose tissue metabolism and fecal microbiota in insulin-resistant subjects with the MetS.Methods:A total of 28 obese men with the MetS were studied during a 35-day stay in the Rockefeller University Hospital metabolic unit.Subjects were randomized to receive RES 1 g orally twice daily or placebo while kept weight stable and consuming a western-style diet.At baseline,and after 30 days of RES or placebo administration,subjects underwent testing that included a euglycemic,hyperinsulinemic clamp,2-h oral glucose tolerance test(GTT),resting energy expenditure,daily blood pressure monitoring,abdominal adipose tissue biopsy,and fecal and blood collections.Results:RES induced no changes in insulin resistance but reduced the 120-min time point and the area under the curve for glucose concentration in the 2-h GTT.In post-hoc analysis,Caucasian subjects showed a significant improvement in insulin sensitivity and glucose homeostasis after GTT,whereas non-Caucasians showed no similar effects.Levels of fasting plasma RES and its primary metabolite dihydroresveratrol were variable and did not explain the racial differences in glucose homeostasis.RES administration to Caucasian subjects leads to an increase in several taxa including Akkermansia muciniphila.Conclusions:RES 2 g administered orally to obese men with MetS and insulin resistance marginally altered glucose homeostasis.However,in a small group of Caucasians,insulin resistance and glucose homeostasis improved.No concomitant changes in adipose tissue metabolism occurred,but fecal microbiota showed RES-induced changes.Relevance for Patients:The MetS increases the risk of diabetes,heart disease,and stroke.A major component of the syndrome is insulin resistance,resulting in systemic inflammation and hyperinsulinemia.The primary treatment consists of lifestyle changes,improved diet,and increased physical activity.This is often unsuccessful.In this study,RES was well tolerated.In Caucasian men,it significantly improved insulin sensitivity and glucose homeostasis.Similar results were found in studies that consisted exclusively of Caucasian men.However,RES presents a novel addition to the current treatment of the MetS and its sequelae. | Jeanne M.Walker Patricia Eckardt Jose OAleman Joel Correa da Rosa Yupu Liang Tadasu Iizumi Stephane Etheve Martin JBlaser Jan LBreslow Peter RHolt | 2018 | Journal of Clinical & Translational Research2018，4，2： | 1 |
| 8 | Pain control after dental surgery:a double blind,randomized trial of lornoxicam verse morphine显示文摘 | Nф rholt SE Sindet-Pedersen S Larsen U | 1996 | Pain1996，67，： | 1 |
| 9 | Pain conrrol after dental sugery: a double-blind, randomised trail of lomoxicam versus morphine显示文摘 | N Rholt SE Sindet-Pedersen U | 1996 | Pain1996，67，： | 1 |
| 10 | The water curtain--A successful means of preventing gas leakage from high-pressure,unlined rock caverns显示文摘 | KJ?RHOLT E BROCH H | 1992 | Tunnelling and Underground Space Technology1992，7，2： | 1 |
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