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| 1 | Methodologic research on TIMP-1,TIMP-2 detection as a new diagnostic index for hepatic fibrosis and its significance显示文摘AIM: To set up a new method to detect tissue inhibitors ofmetalloproteinase1 and -2(TIMP-1 and TIMP-2) in sero ofpatients with hepatic cirrhosis, and to investigate theexpression and location of TIMP-1 and TIMP-2 in liver tissueof patients with hepatic cirrhosis, and the correlationbetween TIMPs in liver and those in sera so as to discusswhether TIMPs can be used ss a diagnosis index of hepaticfibrosisMETHODS: The monoclonal antibodies (McAbs) of TIMP-1and TIMP-2 were used to sensitize erythrocytes, and solid-phase absorption to sensitized erythrocytes (SPASE) wasused to detect TIMP-1 and TIMP-2 in the sera of patients withhepatic cirrhosis. Meanwhile, with the method of in situhybridization and immunohistochemistry, we studied themRNA expression and antigen location of TIMP-1 and TIMP-2in the livers of 40 hepatic cirrhosis patients with pathologicdiagnosis.RESULTS: With SPASE, they were 16.4 % higher in theacute hepatitis group, 33.3 % higher in the chronic hepatitisgroup, and the positive rates were 73.6 % and 61. 2 %respectively in sero of hepatic cirrhosis patients, which wereremarkably higher than those in chronic hepatitis and acutehepatitis group ( P < 0. 001 ). In 40 samples of hepaticcirrhosis tissues, all of them showed positive expression ofTIMP-1 and TIMP-2 mRNA detected withimmunohistochemistry or in situ hybridization (positive ratewas 100 % ). Expression of TIMPs in different degrees couldbe found in liver tissue with cirrhosis. TIMPs were located incytoplasm of liver cells of patients with hepatic cirrhosis.There was a significant correlation between serum TIMPslevel and liver TIMPs level.CONCLUSION: SPASE is a useful method to detect the TIMP-1 and TIMP-2 in sera of patients with hepatic cirrhosis, andTIMP-1 and TIMP-2 can be considered as a useful diagnosticindex of hepatic fibrosis, especially TIMP-1. | Oing-He Nie Yong-Oian Cheng Yu-Mei Xie Yong-Xing Zhou Bai-Xian Guang Yi-Zhan Cao,The Centre of Diagnosis and Treatment for Infectious Disease of Chinese PLA,Tangdu Hospital,Fourth Military Medical University,Xi’an 710038,Shanxi Province,China | 2002 | World Journal of Gastroenterology2002，8，2： | 51 |
| 2 | 不同质子泵抑制剂治疗反流性食管炎的疗效分析显示文摘目的比较不同剂量质子泵抑制剂治疗反流性食管炎(RE)的治愈率。方法收集前瞻性随机对照临床试验的摘要和全文,提取相关数据,采用固定效应模型进行分析。结果埃索美拉唑40 mg短期治疗(4周)RE的治愈率高于奥美拉唑20mg(RR:1.14;95%CI为1.10~1.18)。奥美拉唑、泮托拉唑、兰索拉唑、雷贝拉唑治疗RE的治愈率比较,均无明显差异。结论埃索美拉唑短期治疗RE的疗效优于泮托拉唑、兰索拉唑及雷贝拉唑。 | 夏春生 刘华蓉 达世平 沈微 | 2011 | 重庆医学2011，40，18： | 26 |
| 3 | Expression of insulin-like growth factor Ⅱ and its receptor in hepatocellular carcinogenesis显示文摘INTRODUCTIONInsulin-like growth factor Ⅱ(IGF-Ⅱ) is a mitogenic peptide of 74 kD and is mostly synthesized in fetal liver tissue .IGF-Ⅱ is believed to play an important role in fetal growth and development and is involved in cellular proliferation and differentiation[1-5]. Recently ,several researchers have reported increased expression of the IGF-Ⅱgene in human hepatocellular carcinoma (HCC) and adjacent non-cancerous liver tissues [6-10]. | Zi Rong Fan Dong Hua Yang Jun Cui Han Rong Qin Chun Chi Huang Department of Gastroenterology, Zhujiang Hospital. The First Military Medical University, Guangzhou 510282.Guangdong Province. China | 2001 | World Journal of Gastroenterology2001，7，2： | 24 |
| 4 | Extraction and purification of TGFβ and its effect on the induction of apoptosis of hepatocytes显示文摘AIM To extract and purify the transforming growth factor β (TGF β), and to demonstrate its biological activity in vivo and induction of apoptosis of hepatocytes in vitro.METHODS TGF β was isolated from fresh bovine platelets by acid/ethanol extraction method and purified with ion exchange and gel chromatography. The extracted TGF β was injected subcutaneously to mice, and its biological activity in vivo was observed 72 hfs post-injection by HE staining. The morphological changes were observed by HE staining and the occurrence of apoptosis was detected by TUNEL method after the human normal hepatic cell line QZG was treated with 8 μg@L 1 TGFβ for 12 hrs in vitro.RESULTS The molecular mass 25 ku TGF β protein was successfully extracted. It was able to induce localized granulation tissue formation in vivo. TGF β-treated hepatocytes showed obvious apoptotic morphological changes, including the pyknosis and dense-stained nuclei and cytoplasm, the fragmentary, annular or crescent nuclei, and the 'bubbling' cytoplasm. Moreover, its apoptotic rate was significantly higher than that of the control group (P<0.05).CONCLUSION Biological active TGF β protein is extracted and purified successfully from bovine platelets, and it is able to induce the apoptosis of hepatocytes. | Xiao-Hui Si Lian-Jun Yang Research Institute of Stomatology,the Ninth People’s Hospital,Shanghai Second Medical University,Shanghai 200011,ChinaDepartment of Pathology,Fourth Military Medical University,Xi’an 710033,Shaanxi Province,China | 2001 | World Journal of Gastroenterology2001，7，4： | 15 |
| 5 | 生长因子受体反义基因逆转肝癌细胞的恶性表型显示文摘目的应用反义技术原理将生长因子受体反义基因,导入人肝癌SMMC-7721细胞,阻断肝癌细胞生长因子与其受体的相互作用,研究其对肝癌细胞恶性表型的逆转作用。方法构建人胰岛素样生长因子Ⅱ型受体(IGF-ⅡR)正、反义基因重组真核表达质粒,用磷酸钙-DNA共沉淀方法转染人肝癌细胞株SMMC-7721,经G418培养基筛选得到稳定的抗性细胞。采用Northern杂交检测反义基因转染细胞内源性IGF-ⅡR基因mRNA转录的抑制,同时观察转染细胞克隆形成率,细胞生长曲线和软琼脂生长能力的变化。结果 IGF-ⅡR反义基因转染后,肝癌细胞的内源性IGF-ⅡR基因mRNA转录受到有效抑制;各组转染细胞克隆形成率分别为:空载体组22.0±3.6,正义组13.3±2.1,反义组4.3±2.1,反义基因转染细胞克隆形成率明显降低;软琼脂培养基细胞集落形成数为:SMMC-7721组254.8±15.9,空载体组196.4±30.4。正义组99.4±23.0,反义组0.0±0.0,说明反义基因转染细胞失去在软琼脂上生长的能力;但细胞生长曲线无明显变化。结论应用反义策略干扰肝癌细胞生长因子受体基因的异常高表达状态,可以有效阻断配体与受体间作用的生长刺激信号环路,一定程度地抑制肝癌细胞的恶性表型。 | 徐迪雄 陈文生 叶治家 | 2001 | 世界华人消化杂志2001，9，2： | 12 |
| 6 | Asthma and gastroesophageal reflux disease: Effect of long-term pantoprazole therapy显示文摘AIM: To define the prevalence of gastroesophageal reflux disease (GERD) in mild persistent asthma and to value the effect of pantoprazole therapy on asthmatic symptoms.METHODS: Seven of thirty-four asthmatic patients without GERD served as the non-GERD control group.Twenty-seven of thirty-four asthmatic patients had GERD (7/27 also had erosive esophagitis, sixteen of them presented GERD symptoms. An upper gastrointestinal endoscopy was performed in all the subjects to obtain five biopsy specimens from the lower 5 cm of the esophagus. Patients were considered to have GERD when they had a dilation of intercellular space (DIS)>0.74 μm at transmission electron microscopy.Patients with GERD were treated with pantoprazole,80 mg/day. Forced expiratory volume in one second (FEV1) was performed at entry and after 6 mo of treatment. Asthmatic symptoms were recorded. The required frequency of inhaling rapid acting 32-agonists was self-recorded in the patients' diaries.RESULTS: Seven symptomatic patients presented erosive esophagitis. Among the 18 asymptomatic patients, 11 presented DIS, while all symptomatic patients showed ultrastructural esophageal damage.Seven asymptomatic patients did not present DIS. At entry the mean of FEV1 was 1.91 L in symptomatic GERD patients and 1.88 L in asymptomatic GERD patients.After the treatment, 25 patients had a complete recovery of DIS and reflux symptoms. Twenty-three patients presented a regression of asthmatic symptoms with normalization of FEV1. Four patients reported a significant improvement of symptoms and their FEV1 was over 80%.CONCLUSION: GERD is a highly prevalent condition in asthma patients. Treatment with pantoprazole (80 mg/day)determines their improvement and complete regression. | Calabrese Carlo Fabbri Anna Areni Alessandra Scialpi Carlo Zahlane Desiree Di Febo Giulio | 2005 | World Journal of Gastroenterology2005，11，48： | 10 |
| 7 | 肝病患者血清IGF-Ⅱ水平与HA,PⅢP的关系显示文摘目的 :探讨了肝病患者血清IGF -Ⅱ水平及临床意义。方法 :采用放射免疫分析 118例肝病患者血清中IGF -Ⅱ和HA、PⅢP含量 ,并与 30名正常人作比较。结果 :肝病患者血清中IGF水平非常显著地高于正常人组(P <0 0 1) ,尤以肝癌组为甚。且与HA、PⅢP呈明显的正相关。结论 :血清中IGF -Ⅱ测定有助于肝癌和肝硬化患者的病情观察和预后判断 ,具有一定的临床价值。 | 杜爱玲 冯岚 李红 何浩明 | 2004 | 放射免疫学杂志2004，17，3： | 8 |
| 8 | 肝癌和癌旁肝组织中IGF-Ⅱ的表达及突变分析显示文摘胰岛素样生长因子Ⅱ(Insulin-like growth factor Ⅱ,IGF-Ⅱ)主要由肝脏合成和分泌,在人肝癌和癌旁肝组织中存在 IGF-Ⅱ基因的异常过量表达,可能通过自分泌或邻分泌循环短路在肝癌的发生发展中起某种直接作用,陈思红 etal 在人肝癌中发现了 IGF-Ⅱ cDNA 变异体的存在,但 IGF-Ⅱ的异常过量表达机制尚不明确.我们采用原位杂交技术和银染PCR-SSCP 方法,检测 IGF-Ⅱ转录水平的表达,并分析其基因结构改变,旨在探讨 IGF-Ⅱ表达的规律及其在肝细胞癌变过程中的意义. | 范子荣 杨冬华 覃汉荣 | 2000 | 世界华人消化杂志2000，8，6： | 5 |
| 9 | 五种恶性肿瘤血清IGF-Ⅱ水平及意义显示文摘目的 :探讨恶性肿瘤患者血清IGF -Ⅱ水平及临床意义。方法 :采用放射免疫分析法检测胃癌、肺癌、肝癌、卵巢癌、子宫内膜癌患者血清IGF -Ⅱ ,并与肝硬化、子宫肌瘤和正常对照组进行比较。结果 :胃癌、肺癌、肝癌患者血清IGF -Ⅱ水平明显高于正常对照组 (p<0 0 1) ,肝硬化患者明显高于正常对照组 (p <0 0 1) ,但与肝癌患者比较则明显降低 (p <0 0 1) ;卵巢癌、子宫内膜癌、子宫肌瘤患者与正常对照组比较均无显著性差异 (p >0 0 5 )。结论 :血清IGF -Ⅱ测定对于子宫内膜癌、卵巢癌和子宫肌瘤患者不具临床应用价值 ,但有助于胃癌、肺癌、肝癌及肝硬化患者病情观察和预后判断。 | 齐法莲 徐军 杜秀敏 克丙坤 杨道理 | 2002 | 放射免疫学杂志2002，15，5： | 5 |
| 10 | Gene expression profiles in gastric mucosa of sleep deprivation rats显示文摘INTRODUCTIONStress has been shown to induce gastric mucosallesions and lower the effectiveness of the mucosa asa barrier.In rats,gastric ulcers can beproduced by cold-restraint stress and it isfrequently employed as a model for the study of themechanisms of stress on ulcer formation.Cold-restraint stress however is not normally | Jenny F.L.Chow Marcel W.L.Koo Chi-Hin Cho | 2000 | World Journal of Gastroenterology2000，6，5： | 4 |
| 11 | 实验性肝癌形成过程C-myc IGF-Ⅱ基因与CyclinD1的表达显示文摘目的观察DEN诱发大鼠肝癌过程中C-myc、胰岛素样生长因子Ⅱ(IGF-Ⅱ)基因与细胞周期蛋白cyclinD1的表达变化及其相互关系。方法用原位杂交技术及免疫组化方法检测了大鼠肝癌形成过程中C-myc、IGF-Ⅱ mRNA及cyclinD1的表达变化,计算机图象分析检测结果,并进行统计学相关性分析。结果 C-myc、IGF-Ⅱ基因及cyclinD1在诱癌早期即可检出较多阳性信号,并随着大鼠肝癌诱发的进程逐渐增加,三者表达变化相关性分析具有显著意义(c-myc与IGF-Ⅱ基因阳性信号呈正相关(r=0.8125,P<0.001)。两基因与cyclinD1表达变化呈正相关(r=0.6607,P<0.001、r=0.6071,P<0.001)。结论 C-myc、IGF-Ⅱ基因过表达是肝癌发生的早期事件,并对癌细胞转化表型的维持具有一定意义。两基因的激活可能导致cyclinD1的过表达,三者的协同作用对肝细胞过度增殖以至癌变具有重要意义。 | 凌昌全 钱妍 赵江安 金岩 | 2001 | 世界华人消化杂志2001，9，12： | 2 |
| 12 | 单个与多发胆固醇结石患者胆道细菌感染状况及与免疫球蛋白相关性的对照研究显示文摘目的 :多发胆固醇结石患者胆囊炎症程度较高 ,可能与结石形成有关。对多发和单个胆固醇结石患者胆道细菌感染状况及与免疫球蛋白含量的相关性进行对照研究。方法 :用半定量PCR法测定分析 38例胆囊结石患者胆石、胆汁和粘膜的细菌DNA阳性率和菌落数 ,并测定相应胆汁和粘膜IgA、IgG、IgM含量。结果 :单个和多发胆石组的胆汁细菌DNA阳性率分别为 75 .0 %和 73.7% ,胆囊粘膜细菌DNA阳性率分别为6 6 .7%和 6 4 .0 % ,结石核心的细菌DNA阳性率分别为 5 7.1%和 85 .7% ,结石外周细菌阳性率分别为 71.4 %和 85 .7% ,两组间差异均无显著性。两组间胆汁和粘膜IgA、IgG、IgM含量差异无显著性 ,菌落数与免疫球蛋白含量不相关。多发胆石组粘膜细菌DNA阳性者的胆囊粘膜IgA、IgG含量高于阴性者 (P <0 .0 5 )。单个和多发胆石组胆汁胆固醇饱和指数 (CSI)差异无显著性 ,各组内胆汁细菌阳性与阴性者的CSI差异也无显著性。结论 :多发和单个胆固醇结石患者胆囊细菌感染率相似 ,细菌感染不是多发胆固醇结石患者胆囊炎症严重的原因。多发胆固醇结石患者胆囊粘膜细菌与IgA、IgG含量增高有关 ,可能间接参与胆固醇结石的形成过程。 | 朱雷明 蔡端 张延龄 | 2003 | 肝胆胰外科杂志2003，15，1： | 2 |
| 13 | 雷贝拉唑不同剂量和用法对健康人胃内昼夜pH变化的影响显示文摘目的:观察雷贝拉唑不同剂量和用法对健康人昼夜胃内pH的影响。方法:10例健康志愿者接受4次24h胃内pH监测,即口服雷贝拉唑10mg 1次(A组)、10mg 2次(B组)、20mg 1次(C组)以及空白对照(D组),清洗期2周。结果:观察组(A,B,C)用药后胃内pH值较D组均显著增高(P<0.01);胃内pH>4的时间分别为(11.5±6.2)h,(12.9±6.5)h,(14.5±4.6)h(P>0.05)。C组白天胃内pH>4的时间百分比显著高于A和B组(P<0.05);而B组夜间pH值>4的时间百分比显著高于白天(P<0.05)。A,B,C组抑酸有效率分别为80%,80%和90%(P>0.05)。结论:雷贝拉唑的不同剂量和用法均能提高健康人胃内pH,不同剂量和用法有不同的抑酸特点,为临床合理选择剂量和用药方式提供了依据。 | 郭建强 杨崇美 刘斌 刘吉勇 | 2004 | 中国新药杂志2004，13，5： | 2 |
| 14 | HLA-DR13基因与丙型肝炎病毒感染外周血单个核细胞的关系显示文摘目的:探讨人类白细胞抗原(HLA)中的DR13基因对慢性丙型肝炎患者中丙型肝炎病毒(HCV)感染外周血单个核细胞(PBMC)的影响。方法:选择75例已经确诊为慢性丙型肝炎患者,采用PCR-SSP方法检测HLA-DR13基因,用密度梯度离心法分离收集PBMC,按是否携带HLA-DR13基因分为两组,观察HLA-DR13基因与HCV感染PBMC的关系。结果:本组携带HLA-DR13基因者17例,PBMC阳性4例(23.5%),未携带HLA-DR13基因者58例,PBMC阳性36例(62.1%),采用四格表χ2检验,两组差异有统计学意义(χ2=7.85,P<0.01)。结论:HLA-DR13基因可能与HCV感染PBMC有关。 | 石国梁 | 2007 | 临床误诊误治2007，20，5： | 2 |
| 15 | 我院2011~2013年治疗消化系统疾病口服抗酸药用药分析显示文摘目的了解我院2011~2013年治疗消化系统疾病口服抗酸药的使用情况,为临床用药和药品管理提供参考。方法采用销售金额、用药频度(DDDs)及日均费用(DDC)法进行统计分析。结果我院治疗消化系统疾病口服抗酸药销售金额逐年增加,年增长率大于同年全部药品销售金额年增长率;3年中质子泵抑制药(PPI)占抗酸药总金额分别达到92.92%、95.43%和95.44%。结论 PPI在抗酸药中占具主导地位,是因医师与患者更加趋向于疗效好、作用时间长、依从性好、副作用小的药物。PPI类药物价格较高,但随着生活水平的提高及医保的普及,患者也逐步接受该类药物的治疗。 | 陈华 金伟华 赵涛 蒲志强 邓佳 鞠楷 | 2014 | 西南国防医药2014，24，12： | 2 |
| 16 | 伴有MSI的结直肠癌TGF-βRⅡ基因突变的研究显示文摘目的 许多研究表明在伴有微卫星不稳定性(MicroSatellite Instability,MSI)的遗传性结直肠癌中,β-转化生长因子Ⅱ型受体(Transfonning Growth Factor-βTypeⅡReceptor,TGF-βRⅡ)基因突变频繁,极可能是其发生的重要机制;并且TGF-RⅡ基因的(A)_(10)重复序列是MSI的靶点。但在散发性结直肠癌中报道较少,本研究通过对散发性结直肠癌的MSI及TGF-RⅡ基因突变的枪测,以期了解它们在散发性结直肠癌发生中的作用 方法 选取单态性很强的BAT-26微卫星位点,应用PCR等分子生物学手段检测MSI;应用PCR-SSCP-银染法-测序检测RⅡ基因突变 结果 有13例(26%)为MSI,近端(42.11%)明显高于远端者(16.13%)(P<0.05),RⅡ基因的突变率为10%(5/50),表现为RⅡ基因的(A)_(10)重复序列出现一个A的缺失导致框架移位突变(Frame Shift Mutation),伴有MSI的肿瘤中RⅡ突变率为38.46%,所有RⅡ突变者均伴有MSI,而无MSI的肿瘤中均无RⅡ突变(P<0.01) 结论 不仅在HNPCC中,而且在散发性结直肠癌中,TGF-RⅡ基因的(A)_(10)重复序列是MSI的靶点。 | 狄金明 张一楚 顾琴龙 陈蕾 姜保成 董谦 | 2001 | 实用肿瘤学杂志2001，15，2： | 1 |
| 17 | 胃食管反流病的药物治疗显示文摘在简要介绍胃食管反流病发病机理的基础上,对其药物治疗的临床实施和疗效评价进行阐述,为胃食管反流病的临床治疗提供参考。 | 李林芳 刘南植 | 2007 | 药学服务与研究2007，7，4： | 1 |
| 18 | 洛赛克治疗胃食管反流的临床疗效显示文摘目的 评价洛赛克治疗胃食管反流病 (GERD)的疗效。方法 选择我院 1999~ 2 0 0 3年 6月诊断的GERD患者 80例 ,进行内镜检查并分级。将 80例患者随机分成两组 ,每组 4 0例。治疗组口服洛赛克 2 0mg,1~ 2次 /d;对照组口服雷尼替丁 15 0mg,2次 /d。 4周后复查胃镜并观察临床症状改善情况 ,判定疗效并观察药物副作用。结果 两组患者疗效间的差别有显著性意义 (P <0 0 5 ) ;两组治疗后内镜检查结果间差别有显著性意义 (P <0 0 5 )。两组不良反应发生率均为 5 0 % ,均未发现血、尿常规及肝、肾功能异常。结论 洛赛克治疗GERD疗效好 ,副作用少 ,使用方便 ,值得推广应用。 | 张子昭 赵进清 李树林 胡汝东 | 2004 | 中国全科医学2004，7，22： | 1 |
| 19 | 三联疗法治疗胃食管反流病的疗效显示文摘目的探讨三联疗法(雷尼替丁、硫糖铝、西沙比利)治疗胃食管反流病的治疗效果。方法110例胃食管反流病患者随机分为2组:对照组45例和治疗组65例。对照组采用雷尼替丁、西沙比利治疗;治疗组采用雷尼替丁、西沙比利、硫糖铝三联治疗。结果治疗组痊愈46例,有效14例,无效5例,总有效率为92.3%;对照组痊愈28例,有效8例,无效9例,总有效率为80.0%;2组总有效率比较差异有统计学意义(P<0.05)。结论三联疗法治疗胃食管反流病疗效显著。 | 王木生 吴伟平 | 2008 | 实用临床医学(江西)2008，9，7： | 0 |
| 20 | HLAⅡ类DR基因与肝炎显示文摘 | 梁红霞 | 2004 | 河南医学研究2004，13，4： | 0 |
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