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| 1 | 生理性低氧通过HIF1α上调PLOD2参与绒毛外滋养细胞分化显示文摘生理性低氧在胎盘发育及滋养细胞分化中具有重要作用。HIF1α是响应低氧诱导的关键因子之一,但生理性低氧通过HIF1α影响胎盘发育的具体分子机制尚不明确。该研究首先通过生物信息学方法分析了HIF1α的下游靶基因和绒毛外滋养细胞分化过程中的差异表达基因,并筛选出两者交集基因作为HIF1α调控绒毛外滋养细胞分化的潜在靶标基因。实验验证发现,生理性低氧条件下(8% O_(2)),HIF1α和其下游靶基因PLOD2、HPCAL1、SLC16A3、FAM174B、SYDE1均被诱导上调表达,而NREP和CD4表达下调。过表达HIF1α后,上述靶基因被调控并呈现与生理性低氧诱导相同的表达模式。通过体外Transwell侵袭、细胞划痕和绒毛外植体实验发现,敲低靶基因PLOD2后可显著抑制滋养细胞的侵袭、迁移和外植体外延水平,并能进一步阻碍滋养细胞的细胞骨架重塑及滋养细胞上皮–间质转换过程。该研究初步发现,生理性低氧可以诱导HIF1α上调,并进一步介导其下游靶基因PLOD2等的选择性表达,促进EVT分化。 | 李聪 刘太行 王永恒 丁裕斌 李卓航 王应雄 | 2021 | 中国细胞生物学学报2021，43，4： | 4 |
| 2 | SARS-CoV-2侵入因子——ACE2和TMPRSS2在围着床期胚胎和母胎界面存在表达显示文摘在全球肆虐的严重急性呼吸综合征冠状病毒2(severe acute respiratory syndrome coronavirus 2,SARS-CoV-2)导致了大量人群感染,血管紧张素转化酶2(angiotensin-converting enzyme 2, ACE2)是SARS-CoV和SARS-CoV-2的受体蛋白。然而是否存在SARS-CoV-2的垂直传播仍颇有争议。为了探讨SARS-CoV-2垂直传播的潜在风险,我们利用已发表的单细胞转录组数据观察了围着床期胚胎和母胎界面上ACE2和TMPRSS2(编码跨膜丝氨酸蛋白酶2)的转录表达情况。结果显示,ACE2和TMPRSS2在第6天(D6)围着床期胚胎的滋养外胚层(trophectoderm, TE)细胞、妊娠第8周的合胞滋养层(syncytiotrophoblast at 8 weeks of gestation, STB8W)细胞以及妊娠第24周的绒毛外滋养层(extravillous trophoblasts at 24 weeks of gestation, EVT24W)细胞中均存在明显的共表达现象,表明这些细胞类群可能易被SARS-CoV-2感染。在这三个细胞类群中,ACE2阳性表达细胞相对于阴性细胞在自噬和免疫相关过程中存在差异。尽管ACE2的表达水平在围着床期胚胎中没有表现出性别偏差,但是其在D6 TE、第6天围着床期胚胎的原始内胚层(D6 primitive endoderm, D6 PE)细胞以及ACE2阳性表达的STB细胞中存在明显的性别差异。这表明围着床期发育第6天以及妊娠期胚胎对SARS-CoV-2的易感性可能存在性别差异。我们的结果揭示了胚胎移植过程中,围着床期以及妊娠期胚胎面临着SARS-CoV-2的潜在感染风险。 | 陈伟 袁鹏 杨铭 严智强 孔思明 严杰 柳溪溪 陈依东 乔杰 闫丽盈 | 2020 | Engineering2020，6，10： | 3 |
| 3 | 单细胞组学在早期妊娠母胎界面异质性中的研究进展显示文摘母胎界面是胚胎生长发育的重要环境,成功的妊娠有赖于母胎界面微环境的动态平衡。在单细胞组学技术的支持下,早期妊娠母胎界面的异质性问题得到了更深入的阐述与理解,对深刻认识生理及病理妊娠具有重要的意义。本综述旨在分析近年来单细胞组学技术在早期妊娠母胎界面组织异质性中的研究,深入探究母胎界面组织的异质性问题及母胎免疫耐受的潜在机制,为认识及治疗各种病理妊娠提供新的指导方向。 | 金贝贝 杨晓清 | 2022 | 现代妇产科进展2022，31，2： | 2 |
| 4 | 基于基因芯片整合分析筛选及验证BeWo细胞合体化相关调控因子显示文摘目的:合体滋养层细胞(syncytiotrophoblast,STB)是一类多核化上皮细胞位于人类胎盘最外层,由单核滋养细胞(cytotrophoblast,CTB)分化、融合而来,肩负妊娠维持关键激素的分泌和母胎间营养交换的重要职责,也是母胎屏障重要组成部分之一。然而,目前关于胎盘滋养细胞的合体化过程的调控机制仍不明确。本研究即利用生物信息学的方法筛选并初步验证潜在调控绒毛膜癌BeWo细胞合体化调控的关键基因。方法:从GEO数据库检索并下载BeWo细胞合体化前后的基因芯片数据集,并利用生物信息学分析方法对所有相关数据集进行综合分析,筛选出差异表达基因并进行GO及KEGG聚类分析,进一步通过PPI蛋白互作网络寻找节点基因,最后利用qRT-PCR验证候选基因在合体化前后的差异表达。结果:从2组数据中共筛选出137个差异表达基因,其中25个差异基因为2组数据集共有,参与细胞迁移、细胞黏附、激素代谢、MAPK信号通路等。进一步借助蛋白质互作网络分析,得到3个候选基因并通过qRT-PCR验证,结果与信息分析结果一致。结论:本研究为滋养细胞合体化研究提供了潜在靶基因,也为解析其分子调控机制提供了线索。 | 顾珺巍 丁裕斌 付利娟 王永恒 王应雄 刘太行 | 2019 | 重庆医科大学学报2019，44，8： | 1 |
| 5 | Placental Development and Pregnancy-Associated Diseases显示文摘Serving as the interface between the fetal and maternal environments during gestation,the placenta plays critical roles in the protection of the developing fetus and the maintenance of maternal health.The placenta is primarily derived from the embryonic trophectoderm which differentiates into various subtypes of trophoblast cells through villous and extravillous pathways.The interactions among trophoblasts and multiple decidual cells and immune cells at the maternal-fetal interface fundamentally form the functional units of the placenta,which are responsible for blood perfusion and maternal-fetal material exchange,immune tolerance,and the regulation of pregnancy adaptation.Defects in placental development and functional maintenance are in tight association with adverse pregnancy outcomes such as preeclampsia.In this article,we review recent advances on human trophoblast cell differentiation and the construction of placental functional units and discuss the placental and maternal factors that may contribute to the occurrence of preeclampsia. | Xin Yu Hongyu Wu Yun Yang Feiyang Wang Yan-Ling Wang Xuan Shao | 2022 | Maternal-Fetal Medicine2022，4，1： | 1 |
| 6 | 胎盘发育和子痫前期显示文摘胎盘是妊娠期间胎儿和母体之间的桥梁,具有血流灌注、物质交换、免疫耐受和妊娠适应性调节等功能,对保障胎儿发育和母体健康发挥关键作用。胎盘发育不良与子痫前期等妊娠疾病密切相关。在本文中,我们总结了人类滋养层细胞分化和胎盘功能单元构建的最新研究进展,并讨论了可能导致子痫前期发病的因素。 | 朱玥霖 李倩倩 王雁玲 邵璇 | 2022 | 生物医学转化2022，3，4： | 1 |
| 7 | Longitudinal single-cell RNA-seq of hESCs-derived retinal organoids显示文摘Human retina development involves multiple well-studied signaling pathways that promote the genesis of a wide arrange of different cell types in a complex architectural structure.Human embryonic stem cells(hESCs)-derived retinal organoids could recapitulate the human retinal development.We performed single-cell RNA-seq of retinal organoids from 5 time points(D36,D66,D96,D126,D186)and identified 9 distinct populations of cells.In addition,we analyzed the molecular characteristics of each main population and followed them from genesis to maturity by pseudotime analysis and characterized the cell-cell interactions between different cell types.Interestingly,we identified insulin receptor(INSR)as a specifically expressed receptor involved in the genesis of photoreceptors,and pleiothropin(PTN)-protein tyrosine phosphatase receptor type Z1(PTPRZ1)as a mediator of a previously unknown interaction between Müller and retinal progenitor cells.Taken together,these findings provide a rich transcriptome-based lineage map for studying human retinal development and modeling developmental disorders in retinal organoids. | Shaojun Wang Sergio Poli Xiaoliang Liang Guang-Hua Peng | 2021 | Science China(Life Sciences)2021，64，10： | 1 |
| 8 | 类器官及器官芯片技术在体外胎盘模型构建中的研究进展显示文摘胎盘具有物质交换及屏障功能,对维持胎儿生长发育至关重要。胎盘功能异常与多种不良妊娠结局有关。现有的体外胎盘模型均存在一定局限性,无法满足研究需要。目前类器官及器官芯片技术发展迅速,在药物研发、基础医学研究等方面展示出了极大的应用前景。构建自真实胎盘的类器官在细胞表型、基因表达等方面均与源组织具有高度一致性。胎盘芯片可体外模拟胎盘屏障,并在药物的胎盘通透性研究中被广泛应用。构建胎盘类器官芯片,或构建包含胎盘、子宫内膜等成分的多器官芯片,对于生殖及围产医学领域的发展具有重要意义。 | 张晓璐 李金枝 王丽 刘颂平 | 2023 | 中华生殖与避孕杂志2023，43，11： | 0 |
| 9 | 单细胞组学在母胎界面研究领域的应用及进展显示文摘母胎界面在生殖成功中扮演关键角色,阐明妊娠早期母胎界面形成与演化机制,将为部分病理妊娠的病因研究及早期预测提供线索。目前,针对母胎界面的研究多在动物模型及体外细胞系进行,难以准确还原在体条件下细胞构成的关键特征。单细胞转录组测序能够在单个细胞水平揭示基因表达差异,有助于解析母胎界面细胞构成及其分化过程中的调控机制。但同时,单细胞转录组测序也存在一定的限制,如无法揭示基因表达上游调控、丢失细胞空间分布信息,以及易受细胞大小及组织解离效应影响。引入单细胞染色质可及性测序、空间转录组测序在内的单细胞多组学平台,可构建更加完整的母胎界面细胞图谱及通讯网络,有助于对正常及病理妊娠的分子机制进行更加深入的研究。 | 任远 刘俊涛 | 2022 | 中华妇产科杂志2022，57，12： | 0 |
| 10 | Reduced cell invasion may be a characteristic of placental defects in pregnant women of advanced maternal age at single-cell level显示文摘The mechanisms underlying pregnancy complications caused by advanced maternal age(AMA)remain unclear.We analyzed the cellular signature and transcriptomes of human placentas in AMA women to elucidate these mechanisms.Placental tissues from two AMA women and two controls were used for single-cell RNA-sequencing(scRNA-seq).Controls consisted of AMA women who did not experience any pregnancy complications and pregnant women below the age of 35 years without pregnancy complications.Trophoblast cells were obtained from the placentas of another six pregnant women(three AMA women and three controls),and in-vitro transwell assays were conducted to observe the cell invasion ability.Thirty additional samples(from 15 AMA women and 15 controls)were analyzed to verify the specific expression of serine protease inhibitor clade E member 1(SERPINE1).Preliminary study of the role of SERPINE1 in cell invasion was carried out with HTR8-S/Vneo cells.High-quality transcriptomes of 27607 cells were detected.Three types of trophoblast cells were detected,which were further classified into eight subtypes according to differences in gene expression and Gene Ontology(GO)function.We identified 110 differentially expressed genes(DEGs)in trophoblast cells between the AMA and control groups,and the DEGs were enriched in multiple pathways related to cell invasion.In-vitro transwell assays suggested that the invading trophoblast cells in AMA women were reduced.SERPINE1 was specifically expressed in the trophoblast,and its expression was higher in AMA women(P<0.05).Transfection of human SERPINE1(hSERPINE1)into HTR8-S/Vneo trophoblast cells showed fewer invading cells in the hSERPINE1 group.Impaired cell invasion may underlie the increased risk of adverse pregnancy outcomes in AMA women.Abnormal expression of SERPINE1 in extravillous trophoblast(EVT)cells appears to play an important role. | Bin ZHANG Feng ZHANG Fengying LU Jing WANG Wenbai ZHOU Huihui WANG Bin YU | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，9： | 0 |
| 11 | NF-κB/uPA在胎盘植入性疾病患者血清中的表达及意义显示文摘目的探讨核转录因子(NF-κB)、尿激酶型纤溶酶原激活物(uPA)在胎盘植入性疾病患者外周血、脐血中的表达情况及其意义。方法选取住院分娩的胎盘植入性疾病患者30例作为研究对象,15例胎盘粘连患者为胎盘粘连组,15例胎盘植入患者为胎盘植入组,同时选取15例正常待产无并发症并要求行剖宫产手术的患者为对照组。采用ELISA法检测3组患者外周血及脐血中NF-κB、uPA水平的表达。结果3组患者外周血及脐血中NF-κB表达比较,胎盘植入组外周血中NF-κB表达水平明显高于胎盘粘连组及对照组,差异有统计学意义(F=5.5,P<0.05);胎盘植入组脐血中NF-κB表达水平明显高于胎盘粘连组及对照组,差异有统计学意义(F=5.3,P<0.05)。3组患者外周血及脐血中uPA表达比较,胎盘植入组外周血中uPA表达水平明显高于胎盘粘连组及对照组,差异有统计学意义(F=4.11,P<0.05);胎盘植入组脐血中uPA表达水平高于胎盘粘连组与对照组,差异有统计学意义(F=4.57,P<0.05)。结论胎盘植入性疾病患者外周血及脐血中NF-κB/uPA均呈高表达,二者在胎盘滋养细胞向子宫肌层的黏附、增殖及侵袭过程中发挥一定作用,而导致胎盘植入性疾病的发生。 | 卢玉凤 哈春芳 陈华 孙晓彤 段丽君 单龙 李春明 | 2021 | 宁夏医学杂志2021，43，8： | 0 |
| 12 | Decidualization and Related Pregnancy Complications显示文摘Decidualization is the differentiation of endometrial stromal cells into secretory decidual stromal cells.Human decidualization involves some amount of signaling molecules and pathways as well as genetic reprogramming,which is driven by the postovulatory rise in progesterone levels and local cyclic adenosine monophosphate production.Decidualization extends from the primary decidual zone to the secondary decidual zone,and then exits through apoptosis.Evidences support that decidual fibroblasts function as the pool of decidual stromal cells during pregnancy.Decidualization undergoes an acute inflammatory phase,an anti-inflammatory secretory phase to the final recession phase.The decidualization of the inner layer of endometrium,termed decidua,is the most critical determinant of pregnancy success,which can promote placenta formation,modulate immune tolerance,foster resistance to oxidative stress,sense embryo quality,and control labor.Failure to adequate decidualization in terms of hormones,biochemistry,and immunology leads to adverse pregnancy outcomes,including diseases such as preeclampsia,miscarriage,premature labor,repeated implantation failures,and some age-related decline in reproductive capacity.The development of animal models and in vitro culture systems combined with emerging technologies provides a powerful system to explore the mechanism of decidualization.However,decidualization is a dynamic,multi-step process,and translating of current research progress into disease predictions and interventions for pregnancy complications remains to be achieved.The study of periodic regeneration and spontaneous decidualization of the endometrium will be beneficial to the diagnosis and treatment of pregnancy diseases. | Jing Tong Shijian Lv Jieqiong Yang Hongwanyu Li Weiya Li Cong Zhang | 2022 | Maternal-Fetal Medicine2022，4，1： | 0 |
| 13 | 子痫前期模型工具研究进展显示文摘子痫前期是产科领域中一种“胎盘源性疾病”,是一种严重的妊娠并发症。人类和其他哺乳动物胚胎的生长发育过程与胎盘功能密不可分。本综述就子痫前期体外细胞模型、胎盘外植体、类器官模型、啮齿类动物模型和灵长类动物模型的应用进行了总结,以有助于加深对胎盘发育和相关妊娠疾病的理解。 | 刘雨 陈兢思 杜丽丽 陈晴晴 陈敦金 | 2023 | 中华围产医学杂志2023，26，9： | 0 |
| 14 | 单细胞RNA测序在间充质干细胞研究中的应用显示文摘1970年,Friedenstein等[1]在豚鼠的骨髓和脾脏中发现了可以在体外形成成纤维细胞群落、在体内形成异位骨组织的骨祖细胞。对该类细胞的进一步研究显示,它们能够以未分化的状态复制,并具有分化形成多种间充质组织的潜能,包括骨、软骨、脂肪、肌腱、肌肉和骨髓,属于间充质干细胞(mesenchymal stem cells,MSCs)[2]。MSCs是成体干细胞,存在于骨髓、脂肪等多种组织中。国际细胞治疗联合会(International Society for Cellular Therapy,ISCT)提出的体外培养的间充质干细胞表型为:≥95%细胞表达包括CD105、CD73和CD90在内的标志物,≤2%细胞表达包括CD45、CD34、CD14、CD11b、CD19、CD79A以及HLA-DR19[3]。 | 龙笑 李竹君 | 2020 | 医学研究杂志2020，49，5： | 0 |
| 15 | 人类合胞素在妊娠中的研究进展显示文摘合胞素(syncytin)是一类由人类内源性逆转录病毒所编码的膜糖蛋白,在生理和病理情况下都起着至关重要的作用.近年来,合胞素的研究引起了越来越多的学者注意,并且迅速成为一个研究热点.基于目前的研究进展,本文主要介绍合胞素在母-胎界面中的生理功能,及合胞素的相关调控因子和通路,总结其表达异常和不同病理妊娠的关系,探讨合胞素是否可成为病理妊娠诊断的标志物之一. | 刘芬婷 杨子 李蓉 | 2020 | 中华生殖与避孕杂志2020，40，1： | 0 |
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