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| 1 | DNA条形码技术在中药全产业链的应用进展显示文摘DNA条形码技术是通过一段标准的DNA序列进行生物物种鉴定的方法,在中药鉴定领域应用广泛,推动中药鉴定学的'文艺复兴'。中药全产业链包括上游中药种植、采收环节,中游中药材、中药饮片、中成药生产环节,以及下游中药流通与使用环节。DNA条形码技术以其准确、通用、客观的特点在中药全产业链各关键环节发挥作用。在产业链上游,对中药种子种苗、药用植物进行鉴定,保证源头正确;在产业链中游,对中药材、中药饮片以及中成药组方药进行鉴定,保证企业投料正确、临床用药安全;在产业链下游,参与构建中药溯源体系,实现中药'来源可查,去向可追',跟踪中药生产全过程,保障消费者权益,有助于中药监督管理。结合近年来具体研究实例,本文介绍DNA条形码技术在中药全产业链过程的应用,对促进中药产业现代化、推动中药国际化进程具有重要意义。 | 娄千 辛天怡 宋经元 | 2020 | 药学学报2020，55，8： | 22 |
| 2 | Comparative Genome Analysis of Scutellaria baicalensis and Scutellaria barbata Reveals the Evolution of Active Flavonoid Biosynthesis显示文摘Scutellaria baicalensis(S.baicalensis)and Scutellaria barbata(S.barbata)are common medicinal plants of the Lamiaceae family.Both produce specific flavonoid compounds,including baicalein,scutellarein,norwogonin,and wogonin,as well as their glycosides,which exhibit antioxidant and antitumor activities.Here,we report chromosome-level genome assemblies of S.baicalensis and S.barbata with quantitative chromosomal variation(2 n=18 and 2 n=26,respectively).The divergence of S.baicalensis and S.barbata occurred far earlier than previously reported,and a whole-genome duplication(WGD)event was identified.The insertion of long terminal repeat elements after speciation might be responsible for the observed chromosomal expansion and rearrangement.Comparative genome analysis of the congeneric species revealed the species-specific evolution of chrysin and apigenin biosynthetic genes,such as the S.baicalensis-specific tandem duplication of genes encoding phenylalanine ammonia lyase and chalcone synthase,and the S.barbata-specific duplication of genes encoding 4-Co A ligase.In addition,the paralogous duplication,colinearity,and expression diversity of CYP82 D subfamily members revealed the functional divergence of genes encoding flavone hydroxylase between S.baicalensis and S.barbata.Analyzing these Scutellaria genomes reveals the common and species-specific evolution of flavone biosynthetic genes.Thus,these findings would facilitate the development of molecular breeding and studies of biosynthesis and regulation of bioactive compounds. | Zhichao Xu Ranran Gao Xiangdong Pu Rong Xu Jiyong Wang Sihao Zheng Yan Zeng Jun Chen Chunnian He Jingyuan Song | 2020 | Genomics, Proteomics & Bioinformatics2020，18，3： | 10 |
| 3 | bHLH transcription factor SmbHLH92 negatively regulates biosynthesis of phenolic acids and tanshinones in Salvia miltiorrhiza显示文摘Objective:Salvia miltiorrhiza is a valuable herbal medicine with tanshinone and phenolic acid as the main biological active ingredients.The biosynthetic regulation of these bioactive compounds is controlled by a set of transcription factors(TFs).The basic helix-loop-helix(bHLH)transcription factor plays an important role in various physiological and biochemical processes in plants.However,research on bHLH TFs regulating phenolic acid or tanshinone biosynthesis in S.miltiorrhiza is limited.Methods:qRT-PCR was used for gene expression analysis.The subcellular localization of SmbHLH92 was detected by SmbHLHg2-GFP transient transformation into tobacco leaves,and its fluorescence was observed using a confocal laser scanning microscope.The transcriptional activity of SmbHLH92 was confirmed in the AH109 yeast strain.RNA interference hairy roots of SmbHLH92-RNAi transgenic lines were obtained through Agrobacterium-mediated genetic transformation.Ultra performance liquid chromatography(UPLC)was used to detect the changes of phenolic acids and tanshinones.Results:SmbHLH92 is a bHLH transcription factor that is highly expressed in the root and phloem of S.miltiorrhiza.The subcellular localization and transcriptional activity of SmbHLH92 indicated that SmbHLH92 was located in the nucleus and may be a transcription factor.RNA interference(RNAi)of SmbHLH92 in hairy roots of S.miltiorrhiza significantly increased the accumulation of phenolic acid and tanshinone.Quantitative RT-PCR(RT-qPCR)analysis showed the transcription level of genes encoding the key enzymes involved in the phenolic acid and tanshinone biosynthetic pathways was increased in the hairy roots of the SmbHLH92-RNAi transgenic line,comparing with the control line.Conclusion:These data indicate that SmbHLH92 is a negative regulator involved in the regulation of phenolic acid and tanshinone biosynthesis in S.miltiorrhiza. | Jian-hong Zhang Hai-zhou Lv Wan-jing Liu Ai-jia Ji Xin Zhang Jing-yuan Song Hong-mei Luo Shi-lin Chen | 2020 | Chinese Herbal Medicines2020，12，3： | 7 |
| 4 | 林-药种植模式研究进展显示文摘林-药种植是指以现有林地资源和林下生态环境为依托,在林下种植株高较低、喜荫环境的中草药,以充分利用森林空间的种植模式。林-药种植模式在保护森林资源和生态环境方面发挥积极作用,因此林下套种中草药种植模式成为发展林农业的热门之选。本文主要阐明和总结不同林-药种植的模式、林-药种植的影响因素等,以及利用代谢组学和转录组学的数据分析,揭示基因与代谢物和代谢途径之间关系的相关研究进展,以期为林下套种中草药的可持续发展和增加经营林木业农民的经济收益提供参考意见。 | 梁嘉瑜 梁语 马姜明 | 2022 | 广西师范大学学报(自然科学版)2022，40，5： | 4 |
| 5 | Strategy of Systems Biology for Visualizing the “Black Box” of Traditional Chinese Medicine显示文摘Traditional Chinese medicine(TCM)has been practiced for thousands of years in China.TCM formula,usually composed of several or even dozens of herbal medicines,is the main form of TCM practicing,which is extremely complex due to multiple components and therapeutic targets,especially the characteristics of formula compatibility.Thus,it is an enormous challenge for the modernization of TCM.Systems biology is a strategy for investigating the complex interactions between genes,mRNA,proteins,and metabolites by using integrated omics approaches.In recent years,systems biology has been increasingly adopted in TCM study.This review comprehensively summarized status of syndrome and application of TCM formulae in clinical and preclinical studies and discussed the advances of systems biology in TCM research.Then,a'Disease-Syndrome-Formulae-Effect'strategy was proposed for TCM research.Combination of systems biology and'Disease-Syndrome-Formulae-Effect'strategy provided a novel approach to understand the complex interactions among biological systems,drugs,and complex diseases from a network perspective,thus facilitating the modernization of TCM.The objective of this manuscript is to provide comprehensive and up-to-date review on the application of systems biology in TCM research,as well as the perspective of TCM modernization with systems biology. | Yu Gu Gao-Song Wu Hou-Kai Li Wei-Dong Zhang | 2020 | World Journal of Traditional Chinese Medicine2020，6，3： | 3 |
| 6 | 延胡索不同种源抗病性和产量研究显示文摘[目的]筛选霜霉病抗性好、产量高的延胡索种源及研究延胡索产量相关的农艺性状。[方法]在延胡索霜霉病发生时期,调查24个不同种源延胡索霜霉病的病死率和存活植株高度,在采收期测定不同种源延胡索单丛块茎数、单丛块茎重量和折合产量。[结果]单丛块茎重量和单丛块茎数量呈极显著正相关,获得了病死率低的延胡索种源马2012-11。[结论]筛选抗霜霉病的延胡索优良种质,从源头预防这一病害的发生,减少农药的使用,对生产具有重要意义。 | 方莉 钭凌娟 厉永强 吴梅 徐金晶 何晓婵 | 2022 | 园艺与种苗2022，42，10： | 0 |
| 7 | 中药材林下生态种植业发展现状、问题与对策显示文摘林下种植具有投入产出比高,经济、生态效益高等优点,有利于经济社会可持续发展。随着我国中药材种植面积和产量日渐增加,发展中药材林下生态种植不仅可以节约耕地面积,提高中药材质量,保护生态平衡,也符合我国对中药材产业发展的规划。该文主要阐述我国中药材种植业基本现状,分析中药材林下生态种植的优缺点、可行性、必要性及存在的问题,并提出发展生态种植技术、选育优良品种,因地制宜发展道地药材生产等对策。 | 杨洁 刘芳 王思凡 袁宗胜 | 2023 | 海峡科学2023，，10： | 0 |
| 8 | 时珍法物种鉴定原理与研究策略显示文摘天然药物的主要来源包括植物、动物和微生物等生物物种。这些物种的准确鉴定是天然药物研发的基础。本文提出一种全新的物种鉴定方法:时珍法(analysis of whole-genome,AGE),即从物种全基因组寻找特异靶标序列,精准识别特异靶标序列,实现物种准确鉴定的分子诊断方法。笔者详细阐述了时珍法利用不同物种基因组序列必然存在差异的原理实施物种鉴定,提出该方法的实施策略可分为研究和应用两个层面,分析其特点评判该方法具有原理可靠、特异性强、适用性广等优势,进一步论述了在体系构建过程涉及的基因组获取、生物信息分析、数据库构建等三个关键问题。综上所述,本文为生物信息软件和商品试剂盒的后续开发提供理论基础和方法指导,表明时珍法在不同对象、不同学科、不同行业等方面具有巨大应用潜力。 | 甘雨桐 辛天怡 许文杰 郝利军 齐桂红 娄千 宋经元 | 2023 | 药学学报2023，58，8： | 0 |
| 9 | 蓝莓叶提取物对2型糖尿病小鼠糖脂代谢的调节显示文摘为探讨蓝莓叶提取物(bluberry leaf extract, BLE)对2型糖尿病(type 2 diabetic, T2D)小鼠糖脂代谢的调节作用,采用高糖高脂饮食诱导辅以链脲佐菌素腹腔注射建立T2D小鼠模型,将其分为模型组、阳性组(20 mg·kg^(-1)·d^(-1)阿卡波糖)、BLE低中高剂量组(200,400,800 mg·kg^(-1)·d^(-1)BLE),另设正常组.灌胃干预35 d,分析小鼠血清中血糖、胰岛素和血脂水平,肝脏中糖代谢水平,胰脏和肝脏中的抗氧化水平变化,结合苏木精-伊红染色切片和原位末端标记染色切片,评估BLE对T2D小鼠胰脏和肝脏组织病理损伤的缓解作用.结果表明,BLE能显著降低T2D小鼠空腹血糖、餐后2 h血糖、总胆固醇、甘油三酯、低密度脂蛋白胆固醇和丙二醛的水平,提升胰岛素、高密度脂蛋白胆固醇、肝糖原和谷胱甘肽过氧化物酶的水平,增强葡萄糖激酶和总超氧化物歧化酶的活性,抑制葡萄糖-6-磷酸酶的活性,减轻胰脏和肝脏组织的病理损伤.综上所述,BLE具有显著的降血糖和降血脂作用,推测其可通过减少T2D小鼠胰脏和肝脏的氧化应激损伤,保护脏器组织细胞的生理形态结构,从而在提升胰腺胰岛素分泌功能的同时改善肝脏糖代谢功能,完成机体血糖和血脂水平的整体性调节. | 王怡婷 蒲首丞 刘芬芬 赵雯靓 徐丽珊 | 2024 | 浙江师范大学学报(自然科学版)2024，47，1： | 0 |
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