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| 1 | 苏云金芽孢杆菌chiA,chiB全基因的克隆、表达及其序列分析显示文摘以苏云金芽孢杆菌科默尔亚种15A3菌株基因组DNA为模版,用touchdown PCR方法扩增几丁质酶ChiA和ChiB的全基因序列(GenBank登录号:EF103273和DQ512474)。将PCR产物连接pUCm-T克隆载体,获得重组质粒pUCm-chiA和pUCm-chiB,分别转化E.coliXL-Blue。克隆的几丁质酶基因可以利用本身的启动子异源表达各自的蛋白,不需要几丁质作为诱导物。表达的几丁质酶能够分泌到胞外。证明15A3菌株可组成型表达2种几丁质酶。经核苷酸及氨基酸序列分析证明,chiA基因全长1426bp,含有343bp的上游非编码区和1083bp的ORF,编码360个氨基酸。推测成熟蛋白分子量为36kD,只有一个几丁质酶催化域。chiB基因全长2279bp,含有248bp的上游非编码区和2031bp的ORF,编码676个氨基酸。推测成熟蛋白分子量约为70.6kD,具有三个功能域。核苷酸序列分析显示chiA和chiB的启动子所处的位置及转录起始碱基都不相同,-35区相同,而-10区有两个碱基不同,SD序列也不完全一致。 | 陈艳玲 卢伟 陈月华 肖亮 蔡峻 韩苗苗 | 2007 | 微生物学报2007，47，5： | 7 |
| 2 | CHIA和CHI3L1基因变异与汉族儿童哮喘发病的关系显示文摘目的:探讨CHIA和CHI3L1基因的变异与中山市汉族儿童哮喘发病的相关性。方法:采用病例对照研究,利用MassARRAY-IPLEX技术和基质辅助激光解吸电离飞行时间质谱平台(MALDI-TOF-MS)对CHIA基因的rs10494132和CHI3L1的rs946263位点进行基因分型。结果:在中山市汉族儿童中rs10494132检出CC、CT和TT基因型,rs946263检出AA、AG和GG三种基因型。与正常对照相比,哮喘组rs10494132的基因型分布(χ2=8.767,P=0.012)和等位基因分布(χ2=9.749,P=0.002)均具有明显差异,而rs946263基因型分布(χ2=0.472,P=0.790)和等位基因分布(χ2=0.013,P=0.910)均无显著性差异。与CC+CT基因型相比,rs10494132的TT基因型能显著增加哮喘发生的危险性,OR值(95%可信区间)分别为2.093(1.252~3.498)。在哮喘组中,与CC+CT基因型亚组相比,rs10494132的TT基因型亚组IgE浓度(Z=-2.013,P=0.044)明显增高,而嗜酸性粒细胞计数(Z=-0.704,P=0.482)和CRP(Z=-0.488,P=0.626)无明显变化。rs10494132位点的基因型分布在哮喘初次确诊规则治疗前不同病情严重程度分级之间未见明显差异(χ2=0.719,P=0.397)。结论:CHIA基因的rs10494132可能与中山市汉族儿童哮喘的发病有关,TT基因型可能增加血清IgE浓度。 | 黄湘 谭家余 袁春雷 周涛 罗雅玲 | 2013 | 中国免疫学杂志2013，29，7： | 2 |
| 3 | PbrChiA:a key chitinase of pear in response to Botryosphaeria dothidea infection by interacting with PbrLYK1b2 and down-regulating ROS accumulation显示文摘Pear ring rot,caused by the pathogenic fungi Botryosphaeria dothidea,seriously affects pear production.While the infection-induced reactive oxygen species(ROS)burst of infected plants limits the proliferation of B.dothidea during the early infection stage,high ROS levels can also contribute to their growth during the later necrotrophic infection stage.Therefore,it is important to understand how plants balance ROS levels and resistance to pathogenic B.dothidea during the later stage.In this study,we identified PbrChiA,a glycosyl hydrolases 18(GH18)chitinase-encoding gene with high infection-induced expression,through a comparative transcriptome analysis.Artificial substitution,stable overexpression,and virus induced gene silencing(VIGS)experiments demonstrated that PbrChiA can positively regulate pear resistance as a secreted chitinase to break down B.dothidea mycelium in vitro and that overexpression of PbrChiA suppressed infection-induced ROS accumulation.Further analysis revealed that PbrChiA can bind to the ectodomain of PbrLYK1b2,and this interaction suppressed PbrLYK1b2-mediated chitin-induced ROS accumulation.Collectively,we propose that the combination of higher antifungal activity from abundant PbrChiA and lower ROS levels during later necrotrophic infection stage confer resistance of pear against B.dothidea. | Qiming Chen Huizhen Dong Qionghou Li Xun Sun Xin Qiao Hao Yin Zhihua Xie Kaijie Qi Xiaosan Huang Shaoling Zhang | 2023 | Horticulture Research2023，10，10： | 0 |
| 4 | A chromosome-level genome assembly of chia provides insights into high omega-3 content and coat color variation of its seeds显示文摘Chia(Salvia hispanica)is a functional food crop for humans.Although its seeds contain high omega-3 fatty acids,the seed yield of chia is still low.Genomic resources available for this plant are limited.We report the first high-quality chromosome-level genome sequence of chia.The assembled genome size was 347.6 Mb and covered 98.1%of the estimated genome size.A total of 31069 protein-coding genes were predicted.The absence of recent whole-genome duplication and the relatively low intensity of transposable element expansion in chia compared to its sister species contribute to its small genome size.Transcriptome sequencing and gene duplication analysis reveal that the expansion of the fab2 gene family is likely to be related to the high content of omega-3 in seeds.The white seed coat color is determined by a single locus on chromosome 4.This study provides novel insights into the evolution of Salvia species and high omega-3 content,as well as valuable genomic resources for genetic improvement of important commercial traits of chia and its related species. | Le Wang May Lee Fei Sun Zhuojun Song Zituo Yang Gen Hua Yue | 2022 | Plant Communications2022，3，4： | 0 |
| 5 | 不平等与环境两大危机及其应对--世界史分析的方法论意义及历史学家的任务显示文摘文章针对人类面临的不平等和环境恶化两大危机,指出现有对不平等的研究局限于经济学的单一解释,缺乏诸如环境和健康等多视域探讨,建议引入世界史方法对1500年以来的全球不平等进行跨学科的大数据分析和考察。文章介绍了应对不平等和环境危机的一个组织(CHIA)和三大项目,讨论了诸项目的内容和目标;阐明对不平等进行世界史分析的理论框架、基本假设及方法论意义;指出在收集、整理及分析数据上面临的七大挑战;分析现有学科知识在应对两大危机上的缺陷,指出历史学家应作的努力。 | 胡爱群(译) | 2020 | 学术论坛2020，43，2： | 0 |
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