|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Natural Variation in CCD4 Promoter Underpins Species-Specific Evolution of Red Coloration in Citrus Peel显示文摘Carotenoids and apocarotenoids act as phytohormones and volatile precursors that influence plant development and confer aesthetic and nutritional value critical to consumer preference.Citrus fruits display considerable natural variation in carotenoid and apocarotenoid pigments.In this study,using an integrated genetic approach we revealed that a 5;c/s-regulatory change at CCD4b encoding CAROTENOID CLEAVAGE DIOXYGENASE 4b is a major genetic determinant of natural variation in C3 0 apocarotenoids responsible for red coloration of citrus peel.Functional analyses demonstrated that in addition the known role in synthesizing 3-citraurin,CCD4b is also responsible for the production of another important C3 0 apocarotenoid pigment,p-citraurinene.Furthermore,analyses of the CCD4b promoter and transcripts from various citrus germplasm accessions established a tight correlation between the presence of a putative 5'c/s-regulatory enhancer within an MITE transposon and the enhanced allelic expression of CCD4b in C3 0 apocarotenoid-rich red-peeled accessions.Phylogenetic analysis provided further evidence that functional diversification of CCD4b and naturally occurring variation of the CCD4b promoter resulted in the stepwise evolution of red peels in mandarins and their hybrids.Taken together,our findings provide new insights into the genetic and evolutionary basis of apocarotenoid diversity in plants,and would facilitate breeding efforts that aim to improve the nutritional and aesthetic value of citrus and perhaps other fruit crops. | Xiongjie Zheng Kaijie Zhu Quan Sun Weiyi Zhang Xia Wang Hongbo Cao Meilian Tan Zongzhou Xie Yunliu Zeng Junli Ye Lijun Chai Qiang Xu Zhiyong Pan Shunyuan Xiao Paul D.Fraser Xiuxin Deng | 2019 | Molecular Plant2019，12，9： | 18 |
| 2 | ROS-responsive drug delivery systems for biomedical applications显示文摘In the field of biomedicine, stimuli-responsive drug delivery systems(DDSs) have become increasingly popular due to their site-specific release ability in response to a certain physiological stimulus, which may result in both enhanced treatment outcome and reduced side effects. Reactive oxygen species(ROS) are the unavoidable consequence of cell oxidative metabolism. ROS play a crucial part in regulating biological and physiological processes,whereas excessive intracellular ROS usually lead to the oxidation stress which has implications in several typical diseases such as cancer, inflammation and atherosclerosis. Therefore,ROS-responsive DDSs have elicited widespread popularity for their promising applications in a series of biomedical research because the payload is only released in targeted cells or tissues that overproduce ROS. According to the design of ROS-responsive DDSs, the main release mechanisms of therapeutic agents can be ascribed to ROS-induced carrier solubility change, ROS-induced carrier cleavage or ROS-induced prodrug linker cleavage. This review summarized the latest development and novel design of ROS-responsive DDSs and discussed their design concepts and the applications in the biomedical field. | Wenhui Tao Zhonggui He | 2018 | Asian Journal of Pharmaceutical Sciences2018，13，2： | 12 |
| 3 | Biogenic production of DMSP and its degradation to DMS—their roles in the global sulfur cycle显示文摘Dimethyl sulfide(DMS) is the most abundant form of volatile sulfur in Earth’s oceans, and is mainly produced by the enzymatic clevage of dimethylsulfoniopropionate(DMSP). DMS and DMSP play important roles in driving the global sulfur cycle and may affect climate. DMSP is proposed to serve as an osmolyte, a grazing deterrent, a signaling molecule, an antioxidant, a cryoprotectant and/or as a sink for excess sulfur. It was long believed that only marine eukaryotes such as phytoplankton produce DMSP. However, we recently discovered that marine heterotrophic bacteria can also produce DMSP, making them a potentially important source of DMSP. At present, one prokaryotic and two eukaryotic DMSP synthesis enzymes have been identified.Marine heterotrophic bacteria are likely the major degraders of DMSP, using two known pathways: demethylation and cleavage.Many phytoplankton and some fungi can also cleave DMSP. So far seven different prokaryotic and one eukaryotic DMSP lyases have been identified. This review describes the global distribution pattern of DMSP and DMS, the known genes for biosynthesis and cleavage of DMSP, and the physiological and ecological functions of these important organosulfur molecules, which will improve understanding of the mechanisms of DMSP and DMS production and their roles in the environment. | Xiao-Hua Zhang Ji Liu Jingli Liu Guipeng Yang Chun-Xu Xue Andrew R. J. Curson Jonathan D. Todd | 2019 | Science China(Life Sciences)2019，62，10： | 11 |
| 4 | Reductive dechlorination of 2,4-dichlorophenol using nanoscale Fe^0: influencing factors and possible mechanism显示文摘Nanoscale Fe0 was synthesized through a reductive method in this paper. The experiments were per-formed to investigate the reduction of 2,4-dichlorophenol (2,4-DCP) by nanoscale Fe0 under different conditions. The pathways for the reduction of 2,4-DCP by nanoscale Fe0 were discussed. Batch studies demonstrated that the mechanism includes adsorption, dechlorination and cleavage of the benzene ring. Dechlorination, which occurs after 2,4-DCP molecule is adsorbed on the interface of Fe particle, is an interfacial reaction. One or two chlorine atom can be removed from 2,4-DCP to form 2-chlorophenol, 4-chlorophenol or phenol. As the concentration of 2,4-DCP increased, the relative dechlorination ratio decreased. However, the reduced quantities of 2,4-DCP increased. Temperature can influence dechlo-rination rate and pathway. Dechlorination is prior to cleavage of the benzene ring at a higher tempera-ture, but at a lower temperature, adsorption may be the main pathway, and cleavage of the benzene ring may be prior to dechlorination. | CHENG Rong WANG JianLong ZHANG WeiXian | 2007 | Science China Chemistry2007，50，4： | 9 |
| 5 | The role of furin cleavage site in SARS-CoV-2 spike protein-mediated membrane fusion in the presence or absence of trypsin显示文摘Dear Editor,The rapid spread of SARS-CoV-2(also known as 2019-nCoV and HCoV-191),a novel lineage B betacoronavirus(βCoV),has caused a global pandemic of coronavirus disease(COVID-19).It has been speculated that RRAR,a unique furin-like cleavage site(FCS)in the spike protein(S),which is absent in other lineage BβCoVs,such as SARS-CoV,is responsible for its high infectivity and transmissibility. | Shuai Xia Qiaoshuai Lan Shan Su Xinling Wang Wei Xu Zezhong Liu Yun Zhu Qian Wang Lu Lu Shibo Jiang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 8 |
| 6 | Redox-neutral photocatalytic strategy for selective C–C bond cleavage of lignin and lignin models via PCET process显示文摘It remains challenging to achieve the selective cleavage of C–C bonds in lignin or lignin model compounds to produce aromatic products in high yield and selectivity.We have developed a redox-neutral photocatalytic strategy to accomplish this goal in both b-O-4 and b-1 lignin models at room temperature(RT)via proton-coupled electron transfer(PCET)process without any pretreatments of substrate,by adjusting the alkalinity of base to obtain a lignin models/base PCET pair with a bond dissociation free energy close to 102 kcal/mol.Without breaking down C_b–Ccbond and any C–O bonds,this PCET method is 100%atom economy and produces exclusive Ca–C_bbond cleavage products,such as benzaldehydes(up to 97%)and phenyl ethers(up to 96%),in high to excellent yields and selectivities.Preliminary studies indicated that the PCET strategy is also effective for the depolymerization of native lignin at RT,thus providing significantly important foundation to the depolymerization of lignin. | Yinling Wang Yue Liu Jianghua He Yuetao Zhang | 2019 | Science Bulletin2019，64，22： | 8 |
| 7 | tRNA cleavage: a new insight显示文摘Over the past decades,tRNA was found to be a rich hub of RNA modifications such as 1-methyladenosine and 5-methycytosine modifications and others,holding more than half of all modifications occurring in RNA molecules.Moreover,tRNA was discovered to be a source of various small noncoding RNA species,such as the stress induced angiogenin cleaved tRNA halves(tiRNA)or the miRNA like tRNA derived fragments.tRNA cleavage under stress was fist discovered in bacteria and later was found to be conserved across different species,including mammals.Under cellular stress conditions,tRNA undergoes conformational changes and angiogenin cleaves it into 3′and 5′halves.5′tiRNA halves were shown to repress protein translations.tRNA cleavage is thought of to be a cytoprotective mechanism by which cells evade apoptosis,however some data hints to the opposite;that tiRNA are cytotoxic or at least related to apoptosis initiation.tRNA cleavage also was shown to be affected by tRNA modifications via different enzymes in the cytosol and mitochondria.In this review,we will highlight the biology of tRNA cleavage,show the evidence of it being cytoprotective or a marker of cell death and shed a light on its role in disease models and human diseases as well as possible future directions in this field of RNA research. | Sherif Rashad Kuniyasu Niizuma Teiji Tominaga | 2020 | Neural Regeneration Research2020，15，1： | 5 |
| 8 | In vitro cleavage of hepatitis B virus C mRNA by 10-23 DNA enzyme显示文摘BACKGROUND: 10-23 DNA enzyme is one kind of de-oxyribozymes for RNA cleavage. The inhibition effects of 10-23 DNA enzyme on the expression of the HBV C gene in HepG2. 2. 15 cells were demonstrated previously. The aim of this study was to further explore the cleavage activities of 10-23 DNA enzyme targeting at HBV C gene mRNA in vitro. METHODS: 10-23 DNA enzyme named Drz-HBV-C-9 specific to HBV C gene ORF A1816UG was designed and synthesized. HBV C gene mRNA was obtained by the in vitro transcription method. Cleavage activities of Drz-HBV-C-9 were observed in vitro. Values of kinetic parameters including Km,Kcat and Kcat/Km were calculated accordingly. RESULTS: Under the certain cleavage conditions, Drz-HBV-C-9 could efficiently cleave target mRNA at specific sites in vitro. Cleavage products of 109nt plus 191nt were obtained. The kinetic parameters, Km,Kcat and Kcat/ Km for Drz-HBV-C-9, were 1.4 ×10-9 mol, 1.6 min-1 and 1.1 × 109 mol-1 · min-1, respectively. CONCLUSIONS: 10-23 DNA enzyme targeting at HBV C gene mRNA possesses specific cleavage activities in vitro. This would be a potent antiviral strategy with respect to HBV gene therapy. | Wei Hou, Jian-Er Wo, Min-Wei Li and Ke-Zhou Liu Institute of Infectious Diseases, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，4： | 5 |
| 9 | Dysoanthraquinone and 2-demethyldysoanthraquinone from Dysosma majoense显示文摘Two new anthraquinones,dysoanthraquinone and 2-demethyldysoanthraquinone,wereseparated from Dysosma majoense.Both compounds contain methylendioxy at 6—C,7—C.It isvery interesting in terms of biogenetic pathway that they may have been transformed from aryltetralinlignans by means of oxidative cleavage followed by ring closure. | YIN Meng-Long CHEN Zhong-Liang Shanghai Institute of Materia Medica,Academia Sinica,ShanghaiGU Ze-Sheng XIE Yu-Xiang Drug Control Station of Cengong County,Guizhou Province | 1989 | Acta Chimica Sinica English Edition1989，7，5： | 4 |
| 10 | The plant N-degron pathways of ubiquitinmediated proteolysis显示文摘The amino-terminal residue of a protein(or amino-terminus of a peptide following protease cleavage)can be an important determinant of its stability,through the Ubiquitin Proteasome System associated N-degron pathways.Plants contain a unique combination of N-degron pathways(previously called the N-end rule pathways)E3 ligases,PROTEOLYSIS(PRT)6 and PRT1,recognizing non-overlapping sets of amino-terminal residues,and others remain to be identified.Although only very few substrates of PRT1 or PRT6 have been identified,substrates of the oxygen and nitric oxide sensing branch of the PRT6 N-degron pathway include key nuclear-located transcription factors(ETHYLENE RESPONSE FACTOR VIIs and LITTLE ZIPPER 2)and the histone-modifying Polycomb Repressive Complex 2 component VERNALIZATION 2.In response to reduced oxygen or nitric oxide levels(and other mechanisms that reduce pathway activity)these stabilized substrates regulate diverse aspects of growth and development,including response to flooding,salinity,vernalization(cold-induced flowering)and shoot apical meristem function.The N-degron pathways show great promise for use in the improvement of crop performance and for biotechnological applications.Upstream proteases,components of the different pathways and associated substrates still remain to be identified and characterized to fully appreciate how regulation of protein stability through the amino-terminal residue impacts plant biology. | Michael John Holdsworth Jorge Vicente Gunjan Sharma Mohamad Abbas Agata Zubrycka | 2020 | Journal of Integrative Plant Biology2020，62，1： | 4 |
| 11 | Formation of subsurface cracks in silicon wafers by grinding显示文摘Single-crystal silicon is an important material in the semiconductor and optical industries.However,being hard and brittle,a silicon wafer is vulnerable to subsurface cracks(SSCs)during grinding,which is detrimental to the performance and lifetime of a wafer product.Therefore,studying the formation of SSCs is important for optimizing SSC-removal processes and thus improving surface integrity.In this study,a statistical method is used to study the formation of SSCs induced during grinding of silicon wafers.The statistical results show that grinding-induced SSCs are not stochastic but anisotropic in their distributions.Generally,when grinding with coarse abrasive grains,SSCs form along the cleavage planes,primarily the{111}planes.However,when grinding with finer abrasive grains,SSCs tend to form along planes with a fracture-surface energy higher than that of the cleavage planes.These findings provide a guidance for the accurate detection of SSCs in ground silicon wafers. | Jingfei Yin Qian Bai Yinnan Li Bi Zhang | 2018 | Nanotechnology and Precision Engineering2018，1，3： | 3 |
| 12 | Design of artificial nucleases and studies of their interaction with DNA显示文摘The design of artificial nucleases and nuclease mimics has attracted extensive attention and made great progress due to their significant scientific meanings and potential application in the field of gene medicine and molecular biology. This paper reviews recent progress in the investigation of artificial nuclease,including 'bifunctional cooperative catalysis','dinuclear synergistic catalysis','metal-free catalysis' ,and especially,the studies of aza-crown ethers as artificial nucleases and their interaction with DNA. | ZHANG JingJing SHAO Ying WEI Li LI Ying SHENG Xin LIU Fang LU GuoYuan | 2009 | Science China Chemistry2009，52，4： | 3 |
| 13 | Cloning and Functional Characterisation of Carotenoid Cleavage Dioxygenase 4 from Wolfberry显示文摘Carotenoid cleavage dioxygenases(CCDs) are a class of enzymes in plants involved in the biosynthesis of apocarotenoids,such as phytohormones,flavour compounds,and other compounds with yet unknown functions.To date,several CCDs have been functionally characterised in plants,but little is known about the CCD4 members.A carotenoid cleavage dioxygenase 4 gene(LcCCD4) was isolated from the leaves of wolfberry(Lycium chinense) to gain insight into its biological function.Multiple sequence alignment and phylogenetic analyses showed that the deduced amino acid sequence of LcCCD4 shares high homology with that of CCD4 proteins from other plants.Expression analysis using semi-quantitative polymerase chain reaction revealed that LcCCD4 was strongly expressed in leaves and flowers and that the expression level was in accordance with β-carotene concentration.LcCCD4 transcripts in fruits tended to decrease as carotenoids accumulated.Recombinant expression of LcCCD4 cleaved β-carotene to produce P-ionone in in vivo assays.These results show that LcCCD4 is a CCD gene that may be involved in producing aromatic apocarotenoids in leaves and flowers,whereas it may be involved in controlling carotenoid accumulation in fruits. | Xiaowei Tian Jing Ji Gang Wang Chao Jin Cuicui Jia Zhaodi Li | 2017 | Transactions of Tianjin University2017，23，1： | 3 |
| 14 | Effect of Lamb Age and in vitro Culture System of Oocytes on JIVET Technology显示文摘[Objectives] This study was conducted to investigate the effects of lamb age and in vitro culture system of oocytes on the results of juvenile in vitro embryo transfer( JIVET). [Methods]Ten Dorper × small-tailed Han lambs aged 5 to 10 weeks were induced to superovulate via i. p. injection of pregnant mare's serum gonadotropin( PMSG). The oocytes were matured in basal maturation solution or modified maturation solution,which was prepared by adding 200 μmol/L cysteine to the basal maturation solution. Then,the oocytes were fertilized in fertilization medium I containing 2% estrus sheep serum( ESS) or fertilization medium II containing 3 mg/ml bull serum albumin( BSA). Finally,the number of oocytes,oocyte maturation rate and cleavage rate of the lambs of different ages were determined. [Results]The average number of oocytes recovered per lamb was( 111. 00 ± 16. 97),( 139. 50 ± 28. 99),( 108. 50 ± 17. 68) and( 42. 00 ± 11. 31) for5-,7-,8-and 10-week-old Dorper × small-tailed Han lambs,respectively. The number of oocytes obtained from 5-,7-and 8-week-old lambs was significantly higher than that from 10-week-old lambs( P < 0. 05),but there was no significant difference among 5-,7-and 8-week-old lambs( P > 0. 05). The maturation rate of oocytes cultured in modified maturation solution was 3. 64% higher than that in basal maturation solution. The cleavage rate of oocytes in fertilization medium I was very significantly higher than that in fertilization medium II( P < 0. 01). [Conclusions] The results of JIVET can be improved by harvesting oocytes from lambs aged 5-8 weeks,adding a certain amount of cysteine into oocyte maturation solution,and a certain amount of ESS into fertilization medium. | Yangyi MAO Huidi LUO Guanwu ZHANG Zhiwu WANG Peng ZHAO Jun LI Hongyu GUO Shenghua ZHOU Huihui GUO Chunyan LI | 2019 | Agricultural Biotechnology2019，8，1： | 3 |
| 15 | A novel N-N bond cleavage reaction of 4-amino-1,2,4-triazole derivatives显示文摘5-Substituted-4-amino-3-thiol-1, 2, 4-triazoles (1a- b) react with orthonitrochloro- benzene or para-nitrochlorobenzene to give N-N bond cleavage products 2a-d, one structure of which (2b) has been unambiguously confirmed by an X-ray structural analysis. | 杨光富 杨华铮 | 2000 | Chinese Journal of Chemistry2000，18，3： | 2 |
| 16 | Rapid,Catalytic Hydrolysis of DNA by Copper (Ⅱ) Complexes显示文摘IntroductionNucleaseswhichcatalyzethehydrolyticcleavageofthephosphodiesterbackboneofDNAandRNAplayanimportantroleinthemolecula... | REN Rui, YANG Pin** and GAO Dong (Institute of Coordination Chemistry, State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing, 210093 Institute of Molecular Science, Shanxi University, Taiyuan, 030006) | 1999 | Chemical Research in Chinese Universities1999，15，2： | 2 |
| 17 | Selective Dealkenylative Functionalization of Styrenes via C-C Bond Cleavage显示文摘As a readily available feedstock,styrene with about 25 million tons of global annual production serves as an important building block and organic synthon for the synthesis of fine chemicals,polystyrene plastics,and elastomers.Thus,in the past decades,many direct transformations of this costless styrene feedstock were disclosed for the preparation of high-value chemicals,which to date,generally performed on the functionalization of styrenes through the allylic C-H bond,C(sp2)-H bond,or the C=C double bond cleavage.However,the dealkenylative functionalization of styrenes via the direct C-C single bond cleavage is so far challenging and still unknown.Herein,we report the novel and efficient C-C amination and hydroxylation reactions of styrenes for the synthesis of valuable aryl amines and phenols via the site-selective C(Ar)-C(alkenyl)single bond cleavage.This chemistry unlocks the new transformation and application of the styrene feedstock and provides an efficient protocol for the late-stage modification of substituted styrenes with the site-directed dealkenylative amination and hydroxylation. | Jianzhong Liu Jun Pan Xiao Luo Xu Qiu Cheng Zhang Ning Jiao | 2020 | Research2020，，1： | 2 |
| 18 | Indiscriminate ssDNA cleavage activity of CRISPR-Cas12a induces no detectable off-target effects in mouse embryos显示文摘Dear Editor,Newly discovered characteristics like'collateral effect'or trans-cleavage in CRISPR-Cas13 and CRISPR-Cas12 systems have enabled their usage in nucleic acid detection(Gootenberg et al.2017,2018;Chen et al.2018).The collateral RNA cleavage of Cas13a has been reported to be harmful for cell development(Wang et al.2019;Buchman et al.2020).As a representative gene editor of CRISPR-Cas12 system,CRISPR-Cas12a(Cpf1)holds great potential for therapeutic applications in the future(Zetsche et al.2015;Koo et al.2018;Campa et al.2019). | Yu Wei Yingsi Zhou Yajing Liu Wenqin Ying Ruiming Lv Qimeng Zhao Haibo Zhou Erwei Zuo Yidi Sun Hui Yang Changyang Zhou | 2021 | Protein & Cell2021，12，9： | 2 |
| 19 | Developmental Potential and Clinical Value of Embryos with Abnormal Cleavage Rate显示文摘To investigate the developmental potential and clinical value of embryos with abnormal cleavage rate,a retrospective analysis was performed on 66 635 2-prokaryotic(2PN)and 1-pronuclear(1PN)embryos.The embryos were given conventionally in vitro fertilization(IVF)treatment and continuously cultured on the day 3(D3)at the Reproductive Medicine Center,Tongji Medical College,Huazhong University of Science and Technology from January 2016 to December 2017.The embryos were separated into the day-2(D2)undivided group with 106 cases,the arrested development group with 3482 cases,the blastomere reduction group with 541 cases,and the control group with 62 506 cases,respectively.The blastocyst utilization rates of these three abnormal groups were 2.83%,10.86%and 6.84%,respectively,which were significantly different from that in control group(39.46%).Furthermore,2 cases of anabiosis and 1 case of live birth were found in D2 undivided group.In arrested development group,there were 55 cases of anabiosis,11 cases of clinical pregnancy in single-embryo transplantation(including 6 cases of live birth),and 25 cases of clinical pregnancy in combination with one normal embryo transplantation(including 23 cases of live births and 15 cases of dizygotic twins under B-ultrasound).There were 13 case of anabiosis in blastomere reduction group:there was 1 case of single embryo transplantation and clinical pregnancy was obtained;there were also 6 cases of clinical pregnancy in combination with one single normal embryo transplantation(including 5 cases of live births and 2 cases of di2ygotic twins under B-ultrasound).In conclusion,embryos with abnormal cleavage rate still have the potential to continue to develop,and have certain blastocyst utilization rate and live birth. | Yu-lan WEI Gui-jin ZHU Xin-ling REN Bo HUANG Lei JIN | 2019 | Current Medical Science2019，39，1： | 2 |
| 20 | Biological function of Presenilin and its role in AD pathogenesis显示文摘Presenilins(PSs)are the catalytic core of γ-secretase complex.However,the mechanism of FAD-associated PS mutations in AD pathogenesis still remains elusive.Here we review the general biology and mechanism of γ-secretase and focus on the catalytic components–presenilins and their biological functions and contributions to the AD pathogenesis.The functions of presenilins are divided into γ-secretase dependent and γ-secretase independent ones.The γ-secretase dependent functions of presenilins are exemplified by the sequential cleavages in the processing of APP and Notch;the γ-secretase independent functions of presenilins include stabilizing β-catenin in Wnt signaling pathway,regulating calcium homeostasis and their interaction with synaptic transmission. | Shuting Zhang Mingming Zhang Fang Cai Weihong Song | 2013 | Translational Neurodegeneration2013，2，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员