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| 1 | Effects of glycyrrhetinic acid on collagen metabolism of hepatic stellate cells at different stages of liver fibrosis in rats显示文摘INTRODUCTIONLiver fibrosis is a dynamic course leading tocirrhosis from a various chronic liver diseases. Thepathological basis of fibrosis is the disturbance ofproduction and degradation of the extracellularmatrix (ECM), which causes accumulation of ECMin the liver[1,2]. | Ji Yao Wang Qi Sheng Zhang Ji Sheng Guo Mei Yu Hu Department of Gastroenterology, Zhongshan Hospital, Medical Center, Fu Dan University Shanghai Medical University), Shanghai 200032, China | 2001 | World Journal of Gastroenterology2001，7，1： | 29 |
| 2 | Antitumor effects of the molecule-downsized immunocon-jugate composed of lidamycin and Fab' fragment of monoclonal antibody directed against type IV collagenase显示文摘Type IV collagenase plays an important role in tumor invasion and metastasis through cleaving type IV collagen in the basement membrane and extracellular matrix. In this study a molecule-downsized immunoconjugate (Fab′-LDM) was constructed by linking lidamycin (LDM), a highly potent antitumor antibiotic, to the Fab′ fragment of a monoclonal antibody directed against type IV collagenase and its antitumor effect was investigated. As assayed in 10% SDS-PAGE gel, the molecular weight of Fab′-LDM conjugate was 65 kD with a 1:1 molecular ratio of Fab′ and LDM. The Fab′-LDM conjugate maintained most part of the immunoreactivity of Fab′ fragment to both type IV collagense and mouse hepatoma 22 cells by ELISA. By MTT assay, Fab′-LDM conjugate showed more potent cytotoxicity to hepatoma 22 cells than that of LDM. Administered intravenously, Fab′-LDM conjugate proved to be more effective against the growth of subcutaneously transplanted hepatoma 22 in mice than free LDM in two experiment settings. In Experiment I, the drugs were given intravenously on day 1 and day 8. Fab′-LDM at the doses of 0.025 mg/kg, 0.05 mg/kg and 0.1 mg/kg inhibited tumor growth by 76.7%, 93.3% and 94.8%, while free LDM at 0.05 mg/kg inhibited tumor growth by 76.1%, respectively. In experiment II, the drugs were given intravenously on day 4 and day 11, Fab′-LDM at the doses of 0.025 mg/kg and 0.05 mg/kg inhibited tumor growth by 74.2%, 80.9%, while free LDM at 0.05 mg/kg inhibited tumor growth by 60.5%, respectively. In terms of survival time, Fab′-LDM was more effective than free LDM. The results suggest that the molecule-downsized immunoconjugate directed against type IV collagenase is of high efficacy in experimental cancer therapy. | WANG Fengqiang SHANG Boyang ZHEN Yongsu | 2004 | Science China(Life Sciences)2004，47，1： | 7 |
| 3 | A novel arterial pouch model of saccular aneurysm by concomitant elastase and collagenase digestion显示文摘Background: An ideal aneurysm model of cerebral aneurysm is of great importance for studying the pathogenesis of the lesion and testing new techniques for diagnosis and treatment. Several models have been created in rabbits and are now widely used in experimental studies; however, every model has certain intrinsic limitations. Here we report the development of a novel saccular aneurysm model in rabbits using an arterial pouch that is subject to in vitro pre-digestion with combined elastase and collagenase. Methods: A segment of right common carotid artery (CCA) was dissected out and treated with elastase (60 U/ml, 20 min) followed by type I collagenase (1 mg/ml, 15 min) in vitro. The graft was anastomosed to an arterial arch built with the left CCA and the remaining right CCA, while the other end of the graft was ligated. The dimension and tissue structure of the pouch were analysed immediately, 2 or 8 weeks after operation. Findings: Ten terminal aneurysms were produced. The gross mor-phology of the aneurysm resembles the human cerebral terminal aneurysms. We have observed the following pathological changes: (1) growth of the aneurysm (mean diameter increased from (2.0±0.1) to (3.2±0.3) mm at 2 weeks, P<0.001, n=7~10); (2) thinning of the aneurysmal wall (the mean wall thickness decreased to 44% at 2 weeks), which was accompanied by significant losses of elastic fibres, collagen and the cellular component; and (3) spontaneous rupture (3 out of 9, one aneurysm ruptured 24 h after operation with the other two at 2 and 4 weeks respectively). Conclusion: This rabbit arterial pouch model mimics human cerebral aneurysms in relation to morphology and histology. In particular, this model exhibited an increased tendency of spontaneous rupture. | YANG Xin-jian, LI Li, WU Zhong-xue (Department of Interventional Neuroradiology, Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China) | 2007 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2007，8，10： | 3 |
| 4 | Generation and antitumor effects of an engineered and energized fusion protein VL-LDP-AE composed of single-domain antibody and lidamycin显示文摘Type IV collagenase plays a pivotal role in invasion, metastasis and angiogenesis of tumor. Single domain antibodies are attractive as tumor-targeting vehicle because of their much smaller size com-pared with antibody molecules produced by conventional methods. Lidamycin (LDM) is a potent enediyne-containing antitumor antibiotic. In this study an engineered and energized fusion protein VL-LDP-AE composed of lidamycin and VL domain of mAb 3G11 directed against type IV collagenase was prepared using a novel two-step method. First a VL-LDP fusion protein was constructed by DNA recombination. Secondly VL-LDP-AE was obtained by molecular reconstitution. In MTT assay, VL-LDP-AE showed potent cytotoxicity to HT-1080 cells and KB cells with IC50 values of 8.55×10-12 and 1.70×10-11 mol/L, respectively. VL-LDP-AE showed antiangiogenic activity in chick chrorioallantoic membrane (CAM) assay and tube formation assay. In in vivo experiments, VL-LDP-AE was proved to be more effective than free LDM against the growth of subcutaneously transplanted hepatoma 22 in mice. Drugs were given intravenously on day 3 and 10 after tumor transplantation. Compared in terms of maximal tolerated doses, VL-LDP-AE at 0.25 mg/kg suppressed the tumor growth by 89.5%, LDM at 0.05 mg/kg by 69.9%, and mitomycin at 1 mg/kg by 35%. Having a molecular weight of 25.2 kDa, VL-LDP-AE was much smaller than other reported antibody-based drugs. The results suggested that VL-LDP-AE would be a promising candidate for tumor targeting therapy. And the 2-step approach could serve as a new technology platform for making a series of highly potent engineered antibody-based drugs for a variety of cancers. | MIAO QingFang, SHANG BoYang, OUYANG ZhiGang, LIU XiaoYun & ZHEN YongSu Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China | 2007 | Science China(Life Sciences)2007，50，4： | 3 |
| 5 | Modern treatment strategies for penile prosthetics in Peyronie’s disease:a contemporary clinical review显示文摘Peyronie's disease is a common condition resulting in penile deformity,psychological bother,and sexual dysfunction.Erectile dysfunction is one common comorbid condition seen in men with Peyronie’s disease,and its presence significantly impacts treatment considerations.In a man with Peyronie's disease and significant erectile dysfunction who desires the most reliable treatment,penile prosthesis placement should be strongly considered.In some instances,such as those patients with relatively mild curvature,prosthesis placement alone may result in adequate straightening.However,many patients will require additional straightening maneuvers such as manual modeling,penile plication,and tunica albuginea incision with or without grafting.For patients with severe penile shortening,penile length restoration techniques may also be considered.Herein,we provide a comprehensive clinical review of penile prosthesis placement in men with Peyronie's disease.Specifically,we discuss preoperative indications,intraoperative considerations,adjunctive straightening maneuvers,and postoperative outcomes. | Matthew J Ziegelmann M Ryan Farrell Laurence A Levine | 2020 | Asian Journal of Andrology2020，22，1： | 2 |
| 6 | Production and characterization of a human single-chain Fv to collagenase IV显示文摘The over\|expression of collagenase IV in tumor tissues was found to be closely related to tumor metastasis. Collagenase IV has been therefore considered as one of the novel indicative molecules for tumor diagnosis and treatment. Based on phage display antibody library technique, a single\|chain Fv specific for collagenase IV was successfully cloned. This antibody, referred to as hCo4, was mainly composed of variable regions from heavy and light chains, with its molecular weight of 27 ku. The engineered antibody bound to collagenase IV specifically. The affinity of hCo4 was found to be the same as that of a single\|chain antibody constructed from a monoclonal antibody to collagenase IV. Since hCo4 is the smallest among all the antibodies specific for collagenase IV and it is of human origin, it has a potential to be applied for tumor immunotherapy and for the study of the relationship between collagenase IV and tumor metastasis. | 汤健 阎锡蕴 刘元义 田华松 田波 | 1998 | Science China(Life Sciences)1998，41，4： | 1 |
| 7 | Analysis of isolation of cerebral cortical neurons in rats by different methods显示文摘The aim of this study was to find a way to efficiently separate neuronal cells from the cerebral cortex of adult rats,providing a reference method for rapid acquisition of neuronal cells from the adult rat brain.Fifteen SD rats were randomly divided into three groups,with five SD rats in each group.Then,neuron cells were isolated from the adult rat cerebral cortex by the grinding method,the trypsin method,and the collagenase II method,respectively.The expression of anti-NeuN in the neurons of each group was analyzed by flow cytometry.The acquisition rates and morphology of neurons of each group were observed by immunofluorescence staining.The grinding or collagenase II method is more suitable for rapid acquisition of neuronal cells from an adult rat’s cerebral cortex.The number of neuron cells obtained by the trypsin method were very few,so it is not convenient for later experiments. | JIANHUA LI YAOGANG ZHANG TAO ZHANG MEIYUAN TIAN JING HOU DENGLIANG HUANG YAN CHENG ZHU MAN XIAOMING SU ZHIQIN LI SIXIAN TONG XUAN ZHANG JUN DENG YUN DONG YANYAN MA | 2020 | BIOCELL2020，44，2： | 1 |
| 8 | Intracellular expression of a single-chain antibody directed against type IV collagenase inhibits the growth of lung cancer xenografts in nude mice显示文摘It was documented that type IV collagenase with two subtypes of 72 ku/MMP-2 and 92 ku/MMP-9 plays an important role in tumor invasion and metastasis. The endoplasmic reticulum (ER)- retained, single chain Fv antibody fragment (scFv) was used to inhibit the function of type IV collagenase. For expression in mammalian cells, the assembled scFv M97 gene with ER retention signal encoding 6 additional amino acids (SEKDEL) was reamplified by PCR. The amplified fragments were cloned into the pcDNA3.1 vector. The resulting plasmid was sequenced and then introduced into PG cells, a highly metastatic human lung cancer cell line, by lipofectAMINE method. The result of intrabody gene therapy showed that type IV collegenase expression was down regulated significantly as measured by ELISA. The biological behavior of PG cell, such as the ability of in vitro invasion through Matrigel, colony formation on soft agar, was also inhibited by scFv M97 transfection. Animal experiments in a xenograft model of human lung | 王维刚 张胜华 李毅 徐琳娜 周京华 甄永苏 | 2000 | Science China(Life Sciences)2000，43，4： | 0 |
| 9 | Insight into the spoilage heterogeneity of meat-borne bacteria isolates with high-producing collagenase显示文摘Chilled chicken is inevitably contaminated by microorganisms during slaughtering and processing,resulting in spoilage.Cutting parts of chilled chicken,especially wings,feet,and other skin-on products,are abundant in collagen,which may be the primary target for degradation by spoilage microorganisms.In this work,a total of 17 isolates of spoilage bacteria that could secrete both collagenase and lipase were determined by raw-chicken juice agar(RJA)method,and the results showed that 7 strains of Serratia,Aeromonas,and Pseudomonas could significantly decompose the collagen ingredients.The gelatin zymography showed that Serratia liquefaciens(F5)and(G7)had apparent degradation bands around 50 kDa,and Aeromonas veronii(G8)and Aeromonas salmonicida(H8)had a band around.65 and 95 kDa,respectively.The lipase and collagenase activities were detected isolate-by-isolate,with F5 showing the highest collagenase activity.For spoilage ability on meat in situ,F5 performed strongest in spoilage ability,indicated by the total viable counts,total volatile basic nitrogen content,sensory scores,lipase,and collagenase activity.This study provides a theoretical basis for spoilage heterogeneity of strains with high-producing collagenase in meat. | Haodong Wang Liangting Shao Jinhao Zhang Xinglian Xu Jianjun Li Huhu Wang | 2024 | Food Science and Human Wellness2024，13，3： | 0 |
| 10 | Comparison of metastatic potentiality and features related to metastasis in human colorectal carcinoma cell lines显示文摘Objective To compare metastatic potentiality and features related to metastasis in two kinds ofhuman colorectal carcinorna (HCC) cell lines. Methods: Experimental metastatic potentiality of two kinds ofcell lines, HT29 and LoVo cells were compared by using nude mice model for human colorectal carcinoma liver metastasis. The expressions of epithelial-cadherin (E-cad), laminin receptor(LNR) and type Ⅳcollagenase(Ⅳ col) in two kinds of HCC cell lines, HT29 and LoVo cells, were observed quantitatively. The intracellular CD44V6 expressed in mRNA and in protein of the HCC cell lines were detected with in situ hybridization and immunohistochemistry. Results: 100% of nude mice had liver metastasis, which demonstrated thatLoVo is a high metastatic HCC cell line. However, HT29 is a relatively low metastatic HCC cell line becauseonly 42. 9 % of nude mice transplanted HT29 cells had liver metastasis. The expressions of E--cad, LNR andIVcol of HT29 and LoVo cells in vitro were detected quantitatively. HT29 cells expressed E-cad with weakpositive on cell membrane. and LoVo cells almost expressed none. LoVo cells expressed higher level of LNRand tV col than HT29 cells did. The expression of CD44V6 in LoVo cells whether in mRNA or in protein werehigher than that of HT29 cells. Conclusion: LoVo is a high metastatic HCC cell line while HT29 is a relatively low metastatic HCC cell line. E-cadherin, LNR, IV col and CD44V6 can determine the metastatic potentialof HCC cells. | 邓永键 李祖国 邱红明 黄卓垣 丁彦青 朱梅刚 | 1998 | Journal of Medical Colleges of PLA(China)1998，13，3： | 0 |
| 11 | Quantitative investigation of invasive effects of laminin-receptorantibodies on human colorectal carcinoma cell lines显示文摘Objective To investigate the relationship between expression of type Ⅳ collagenase (Ⅳ col ) andthe invasion of laminin-receptors (LNR) in human colorectal carcinoma cells. Methods: The expression ofLNR and Ⅳ col in human colorectal carcinoma(HCC) cell lines were detected quantitatively with different potential metastasis. By using human amniotic membrane(HAM) invasion model, we observed the expressionof Ⅳ col and invasion in both HCC cell lines affected by LNR monoclonal antibodies. Results: High metastaticpotential cell line Lovo expressed higher levels of LNR and Ⅳ col than HT29, a low metastatic potential HCCcell line. The number of cells adhered to HAM of Lovo were more than that of HT29. When the cells of thetwo HCC cell lines blocked by LNR antibodies, the expression of Ⅳcol were decreased, and the cells adheredto HAM expressed Ⅳ col were also decreased. Conclusion: LNR might affect the regulation of expression ofⅣ col in tumor cells by cellular signal communication, and then affect the invasion and metastasis of tumor. | 邓永键 邱红明 李祖国 黄卓垣 丁彦青 | 1998 | Journal of Medical Colleges of PLA(China)1998，13，3： | 0 |
| 12 | A randomized clinical trial assessing the efficacy of single and multiple intralesional collagenase injections for treating contracted scars显示文摘Background:Scar contractions caused by trauma or burns can cause secondary physical dysfunction and disfigurement.Many minimally invasive methods for scar contraction have shown limited applicability and efficacy.This study investigated the feasibility and efficacy of intralesional collagenase injections for scar contraction treatment.Methods:Patients with contracted scars who had limited joint movement and physical disfiguration for>1 year were enrolled in this single-blind,randomized clinical trial from July 2017 to February 2018 at Shanghai Ninth People’s Hospital.Collagenase was injected into the firm-contracted scar(15 U/cm^(2))three times at 4-week intervals in the multiple treatment group and once in the single treatment group,and a placebo injection was performed in the control group.Scar length and skin texture were documented at the 4-and 12-week follow-ups.The safety of the collagenase treatment was also evaluated.Results:The contracted scar was significantly elongated after both single and multiple collagenase treatments.The results showed that,compared to a one-time treatment,repeated injections were more effective at 12 weeks,with an average improvement of 26.83(15.79%).At 12 weeks,78.9% of the patients in the multiple group and 52.9%in the single group achieved significant improvement at 12 weeks.No severe adverse events were observed.Conclusion:Intralesional collagenase injection showed promising results in improving scar contraction and provides an alternative treatment for patients. | Shuangbai Zhou Poh-Ching Tan Cheng-An Chiang Yun Xie Peiqi Zhang Qingfeng Li Kai Liu | 2023 | Chinese Journal Of Plastic and Reconstructive Surgery2023，5，3： | 0 |
| 13 | Anti-Angiogenesis and Anti-Tumor Effect of Shark Cartilage Extract显示文摘The effect of shark cartilage extract (SCE), purified in this laboratory, on angiogenesis in chick chorioallantoic membrane (CAM), on the activity of collagenase IV and on human umbilical vein endothelial cell (ECV 304) proliferation and apoptosis was investigated in vitro. The results showed that SCE caused a decline in CAM blood vessels and significantly prevented collagenase induced collagenolysis. Moreover, SCE produced a dose dependent decline in ECV 304 proliferation and altered its normal cell cycle. These results suggest that the anti angiogenesis and anti tumor effects of shark cartilage may be due to inhibition of endothelial cells as well as collagenolysis. | 王锋 王漪涛 谢莉萍 张荣庆 | 2001 | Tsinghua Science and Technology2001，6，3： | 0 |
| 14 | Use of intralesional collagenase in the treatment of peyronie's disease: A review显示文摘AIM:To review the relevant literature in an effort to examine the body of evidence available to date.METHODS:Ovid MEDLINE search database was queried using MeS H terms'penile induration','peyronie’s disease','Collagenases'and'Collagenase'using various permutations.No temporal parameters were employed.RESULTS:In all,5 relevant clinical trials were isolated from 34 results.These trials were analyzed using the Oxford Centre for Evidence-Based Medicine criteria.They were further examined based on study design and methods;the primary and secondary outcomes were reviewed for treatment efficacy and collagenase-related side effects.CONCLUSION:Intralesional collagenase appears to be safe and effective in the non-surgical treatment of Peyronie’s disease.However,the data remains limited and further inquiries into the safety of collagenase,treatment standardization and standardized outcomesreporting remain necessary.Furthermore,studies comparing intralesional collagenase to alternative medical and surgical therapy will be important in guiding the future treatment decision process. | Daniel Marchalik Henry Wright Frank Hill Anup Vora | 2014 | World Journal of Clinical Urology2014，3，3： | 0 |
| 15 | Therapeutic applications of collagenase(metalloproteases): A review显示文摘Non-invasive therapeutic methods have recently been used in medical sciences. Enzymes have shown high activity at very low concentrations in laboratories and pharmaceutical,enabling them to play crucial roles in different biological phenomena related to living organism, especially human medicine. Recently, using the therapeutic methods based on non-invasive approaches has been emphasized in medical society. Researchers have focused on producing medicines and tools reducing invasive procedures in medical.Collagenases are proteins which catalyze chemical processes and break the peptide bonds in collagen. Collagen may be generated more than the required amount or produced in unsuitable sites or may not degrade after a certain time. In such cases, using an injectable collagenase or its ointment can be helpful in collagen degradation. In both in vitro and in vivo tests, it has been revealed that collagenases have several therapeutic properties in wound healing, burns, nipple pain and some diseases including intervertebral disc herniation, keloid, cellulite, lipoma among others. This review describes the therapeutic application of collagenase in medical sciences and the process for its production using novel methods, paving the way for more effective and safe applications of collagenases. | Hamzeh Alipour Abbasali Raz Sedigheh Zakeri Navid Dinparast Djadid | 2016 | Asian Pacific Journal of Tropical Biomedicine2016，6，11： | 0 |
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