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| 1 | Molecular mechanism about lymphogenous metastasis of hepatocarcinoma cells in mice显示文摘AIM To investigate the correlation between lymphogenous metastasis and matrix metalloproteinases (MMPs) activity and the expression of Fas Iigand of tumor cells in lymph nodes.METHODES Fifty-six inbred 615-mice were equally divided into 2 groups and inoculated with Hca-F and Hca-P cells. Their lymph node metastatic rates were examined.Growth fraction of lymphocytes in host lymph nodes was detected by flow cytometry. The Hca-F and Hca-P cells were cultured with extract of lymph node, liver or spleen.The quantity of MMPs in these supernatants was examined by zymographic analysis. The expression of Fas ligand,PCNA, Bcl-2 protein of Hca-F and Hca-P cells in the mice were examined by immunohistochemistry. The apoptosis signals of macrophages in lymph nodes were observed with in situ DNA fragmentation.RESULTS On the 28th day post-inoculation, the lymph node metastatic rate of Hca-F was 80% (16/20), whereas that of Hca-P was 25% (5/20). The growth fraction of lymphocytes was as follows: in the Hca-F cells, the proliferating peak of lymphocytes appeared on the 14th day post-inoculation and then decreased rapidly, while in HcaP cells, the peak appeared on the 7th day post-inoculation and then kept at a high level. With the extract of lymph node, the quantity of the MMP-9 activity increased (P<0.01) and active MMP-9 and MMP-2 were produced by both Hca-F and Hca-P tumor cells, which did not produce MMPs without the extract of lymph node or with the extracts of the liver and spleen. The expression of Fas Ligand of Hca-F cells was stronger than that of Hca-P cells (P<0.01). The expressions of PCNA and Bcl-2 protein of Hca-F cells in the tumors of inoculated area were the same as that of Hca-P cells. In situ DNA fragmentation showed that the positive signals of macrophages were around HcaF cells.CONCLUSION Secretion of MMPs which was associated with metastatic ability of Hca-F and Hca-P tumor cells depends on the environment of lymph nodes. The increased expression of Fas ligand protein of Hca-F tumor cells with high lymphogenous metastatic potential in lymph nodes may help tumor cells escape from being killed by host lymphocytes. | Li Hou Ying Li Yong-Hua Jia Bo Wang Yi Xin Mao-Ying Ling Shen Lü Department of Pathology,Dalian Medical University,Dalian 116027,Liaoning Province,ChinaDepartment of Biochemistry,Dalian Medical University,Dalian 116027,Liaoning Province,China | 2001 | World Journal of Gastroenterology2001，7，4： | 47 |
| 2 | Experimental and clinic-opathologic study on the relationship between transcription factor Egr-1 and esophageal carcinoma显示文摘AIM To observe the growth suppression effect of exogenous introduction of early growth response gene-1 (Egr-1 gene) on esophageal carcinoma tissue as well as on esophageal carcinoma cell line Eca109 and to explore the potential application of Egr-1 gene in gene therapy of tumor.METHODS Eukaryotic expression vector of PCMV-Egr-1 plasmid was introduced into Eca109 cell line which expressed no Egr-1 protein originally with lipofectamine transfection method. The introduction and expression of PCMV-Egr-1 plasmid into Eca109 cell line was confirmed by G418 selection culture, PCR amplification of neogene contained in the vector, Western blot analysis and immunocytochemical analysis. The cell growth curve,soft agar colony formation rate and tumorigenicity in SClD mice were examined to demonstrate the growth suppression effect of exogenous Egr-1 gene on Eca109 cell line. The Egr-1 mRNA and Egr-1 protein were also detected in 50 surgical specimens of esophageal carcinoma by in situ hybridization and immunohistochemistry.RESULTS Exogenous Egr-1 gene was introduced successfully into Eca109 cell line and expressed Egr-1 protein stably. The transfected Eca109 cell line grew more slowly than control Eca109 as shown by cell growth curves, the soft agar colony formation rate (4.0% vs 6.9%, P<0.01) and the average growth rate of tumor in SCID mice (35.5 ± 7.6 vs 65.8 ± 7.6, P<0.05). The expression level of Egr-1 mRNA and protein significantly increased in dysplastic epithelia adjacent to cancer rather than in cancer tissues (65.8% vs 20.0% by ISH and 57.9% vs 14.0% by IHC, P<0.01).CONCLUSION Exogenous Egr-1 gene shows the strong effect of growth inhibition in Eca109 cell line. Egr-1 in the cancer tissue shows down-regulated expression that supports the inhibited function of Egr-1 in cancer growth and suggests Egr-1 may have an important role in gene therapy of esophageal carcinoma. | Ming-Yao Wu Mao-Huai Chen Ying-Rui Liang Guo-Zhao Meng Huan-Xing Yang Chu-Xiang Zhuang Department of Pathology,Shantou University Medical College,Shantou 515031,Guangdong Province,China Supported by the National Natural Science Foundation of China,No.39670298. | 2001 | World Journal of Gastroenterology2001，7，4： | 20 |
| 3 | Studies on mechanism of Sialy Lewis-X antigen in liver metastases of human colorectal carcinoma显示文摘INTRODUCTIONSialyl Lewis-X antigen ,correlated with carcinoma, is a group of carbohydrate antigen containing oligosaccharide expressed of embryonic tisue and glycoproteins on cell surface of embryonic tissue[1].The SLeX antigen located on cell surface is synthesized principally by two enzymes ,al ,3fucosyltransfrease and a2, 3sialyctransferase.In adults ,SLeX antigen is expressed principally on the surfaces of granulocytic cells and some tumor cells . | Xiao Wei Li~1 Yan Qing Ding~1 Jun Jie Cai~1 Shao Qing Yang~2 Lian Bing An~3 Dong Fang Qiao~3 ~1Department of Pathology,Nanfang Hospital of the First Military Medical University,Guangzhou 510515,Guangdong Province,China ~2The Northern Hospital of PLA,Shenyang 110015,Liaoning Province,China ~3Department of Electronmicroscopy,First Military Medical University,Guangzhou 510515,Gangdong Province,ChinaDr.Xiao Wei Li graduated from the First Military Medical University with a MM degree in 1999.Physician in Charge of pathology,having 6 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 19 |
| 4 | Effect of retinoic acid on the changes of nuclear matrix-intermediate filament system in gastric carcinoma cells显示文摘AIM To explore the relationship between the configuration changes of the nuclear matrix-intermediate filament system in cancer cell induced by retinoic acid and the malignantphenotypic reversion of cancer cells.METHODS The human gastric adenocarcinomacell line MGc80-3 cells were induced with 10-6mol/ L retinoic acid and subouItured at cover slipstrip and gold grids. The cells were treated byselective extraction methOd and prepared for whole mount electron microscopy observation.The samples were examined respectively withscanning and transmission electron microscope.RESULTS The nuclear matrix filaments andintermediate filaments in MGC80-3 cells wererelatively few and scattered, not welldistributed and arranged irregularly. The nuclear lamina was ununiformly thick and compact,connected to the nuclear matrix filaments and intermediate filaments relaxedly. However, thetwo kinds of filaments were abundant and welldistributed, different in slender and thick form and interweaved into a regular network in the cells induced by 10-6 mol/ L RA. The nuclear matrix filaments and intermediate filaments were connected closely by the thin and compact fiber-Iike lamina, and interlaced into a regularnetwork throughout the whole cell region.CONCLUSION The NM-IF system in MGc80-3cells had undergone a restorational changesimilar to those of normaI cells after RAinducement. This alternation is an importantmorpholOgical and functional expression to themalignant phenotypic reversion of cancer cells. | Ll Qi-Fu(Laboratory of Cell Biology, XiamenUniversity, Xiamen 361O05, Fuian Province, China) | 1999 | World Journal of Gastroenterology1999，5，5： | 17 |
| 5 | Experimental study of bioartificial liver with cultured human liver cells显示文摘METHODSTheliversupportexperimentofEBLSSconsistingofaggregatesculturedhumanlivercels,holowfiberbioreactor,andcirculationunit... | WANG Ying Jie, LI Meng Dong, WANG Yu Ming, NIE Qing He and CHEN Guo Zheng | 1999 | World Journal of Gastroenterology1999，5，2： | 14 |
| 6 | Effect of endotoxin on fibronectin synthesis of rat primary cultured hepatocytes显示文摘EfectofendotoxinonfibronectinsynthesisofratprimaryculturedhepatocytesJIAJinBin,HANDeWu,XURuiLing,GAOFei,ZHAOLongFeng,ZHA... | JIA JinBin, HAN DeWu, XU RuiLing, GAO Fei, ZHAO LongFeng, ZHAO YuanChang, YAN JinPin and MA XueHuiKeywords liver cirrhosis endotoxins fibronectin/analysis liver/cytology cells, cultured lipopolysaccharide | 1998 | World Journal of Gastroenterology1998，4，4： | 14 |
| 7 | Moving toward an understanding of the metastatic process in hepatocellular carcinoma显示文摘INTRODUCTIONClinical factors contributing to the therapeutic challenge of hepatocellular carcinoma (HCC) are manifold :tumors arise often in patients with compromised liver function ,therefore limiting therapeutic options ;symptoms develop only at later stages of tumor progression ,and tumors tend to invade normal stuctures or occur in multiple locations simultaneously. | W.Michael Korn Division of Gastroenterology and Comprehensive Cancer Canter,University of California San Francisco,San Francisco,CA 94143-0128,USA | 2001 | World Journal of Gastroenterology2001，7，6： | 13 |
| 8 | Sequencing of p53 mutation in established human hepatocellular carcinoma cell line of HHC4 and HHC15 in nude mice显示文摘INTRODUCTIONAlthoughtheetiopathologyofhumanhepatocelularcarcinoma(hHCC)isstilunknown,alotofevidencestronglysuggestedthatinfec... | YANG Shan Min, ZHOU Hong, CHEN Rui Chuan, WANG Yu Fang, CHEN Fu, ZHANG Chang Gong, ZHEN Yun, YAN Jiang Hua and SU Jin Hua | 1998 | World Journal of Gastroenterology1998，4，6： | 11 |
| 9 | Killing effect of TNF-related apoptosis inducing ligand regulated by tetracycline on gastric cancer cell line NCI-N87显示文摘AIM To clone the cDNA fragment of human TRAIL (TNFrelated apoptosis inducing ligand) into a tetracyclineregulated gene expression system, the RevTet-On system, transduce expression vectors into a gastric carcinoma cell line-NCl-N87 and examine the effects of controlled expression of TRAIL in vitro on the gastric carcinoma cells.METHODS The full-length cDNA of TRAIL was inserted into a vector under the control of the tetracyclineresponsive element (TRE) to obtain the plasmid pRevTRETRAIL, which was transfected into a packaging cell line PT67. In addition, vector pRev-Tet- On and pRevTRE were also transfected into PT67 separately. After hygromycin and G418 selection, the viral titer was determined. The medium containing retroviral vectors was collected and used to transduce a gastric carcinoma cell line NCI-N87.The resulting cell line NCI-N87-Tet-On-TRE-TRAIL and a control cell line, NCI-N87-Tet-On-TRE, were established.TRAIL expression in the cell line was induced by incubating cells with doxycycline (Dox), which is a tetracycline analogue. The killing effect on gastric carcinoma cells was analyzed after induction.RESULTS The recombinant plasmid pRev-TRE-TRAIL was constructed. After hygromycin or G418 selection, the producer cell lines PT67-TRE, PT67-TRE-TRAIL and PT67TetOn were obtained, with titers of about 108CFU@ L-1 By transducing NCI-N87 cells with retroviral vectors from these cell lines, stable cell lines NCI-N87-Tet-On-TRETRAIL (NN3T) and control cell line NCI-N87-Tet-On-TRE (NN2T) were established. The growth curves of the selected cell lines were the same with the wild type NCIN87. When Dox was added, cell death was obvious in the test groups (29% -77%), whereas no difference was observed in control and wild type cell lines. With the addition of a medium from the test group, human leukemia cell line Jurkat was activated till death (83%), indicating the secretion of active TRAIL proteins from the test cells to the medium.CONCLUSION With the use of the RevTet-On system, a regulated expression system for TRAIL was constructed.Using this system, the selected killing effect of TRAIL on gastric carcinoma cell line NCI-N87 could be observed. | Xiao-Chao Wei Xin-Juan Wang Kai-Chen Lei Zhang Yu Liang Xin-Li Lin Department of Biochemistry and Molecular Biology,Peking University Health Science Center,Beijing 100083,ChinaProtein Studies,Oklahoma Medical Research Foundation,Oklahoma City,OK 73104,USA | 2001 | World Journal of Gastroenterology2001，7，4： | 11 |
| 10 | Personalized targeted therapy for esophageal squamous cell carcinoma显示文摘Esophageal squamous cell carcinoma continues to heavily burden clinicians worldwide. Researchers have discovered the genomic landscape of esophageal squamous cell carcinoma, which holds promise for an era of personalized oncology care. One of the most pressing problems facing this issue is to improve the understanding of the newly available genomic data, and identify the driver-gene mutations, pathways, and networks. The emergence of a legion of novel targeted agents has generated much hope and hype regarding more potent treatment regimens, but the accuracy of drug selection is still arguable. Other problems, such as cancer heterogeneity, drug resistance, exceptional responders, and side effects, have to be surmounted. Evolving topics in personalized oncology, such as interpretation of genomics data, issues in targeted therapy, research approaches for targeted therapy, and future perspectives, will be discussed in this editorial. | Xiaozheng Kang Keneng Chen Yicheng Li Jianying Li Thomas A D'Amico Xiaoxin Chen | 2015 | World Journal of Gastroenterology2015，21，25： | 10 |
| 11 | Cloning of the non-structural gene 3 of hepatitis C virus and its inducible expression in cultured cells显示文摘METHODSThens3genewasobtainedfromplasmidpBns3bypolymerasechainreactionandinsertedintothecloningvectorpGEMT.Then,thens3wassubc... | ZHANG Shu Zhong 1, LIANG Jia Jing 1, QI Zhong Tian 2 and HU Yi Ping 1 | 1999 | World Journal of Gastroenterology1999，5，2： | 9 |
| 12 | THE STRUCTURE OF ARMILLARIZIN,A NEW PROTOILLUDANE SESQUITERPENOID AROMATIC ESTER FROM ARMILLARIA MELLEA(VAHL.EX FR)QUEL.显示文摘A new protoilludane sesquiterpenoid aromatic ester,named armillarizin(1)has been isolated from the artificially cultured mycelium of Armillaria me-llea(Vahl.ex Fr)Quel.(Tricholomataceae).Its structure was deduced on the ba-sis of spectral analyses.The relative configuration was established from thenuclear Overhauser effects and from the values of ~1H-~1H coupling constants. | Jun Shan YANG Ya Lun SU Yu Lan WANG Xiao Zhang FENG De Quan YU Xiao Tian LIANG Institute of Materia Medica,Chinese Academy of Medical Sciences,Beijing,100050 | 1990 | Chinese Chemical Letters1990，1，2： | 8 |
| 13 | Study on expresion of adherent proteins related to regulation of hematopoiesis in long-term cultured human bone marrow stromal cells显示文摘Studyonexpresionofadherentproteinsrelatedtoregulationofhematopoiesisinlongtermculturedhumanbonemarrowstromalcels.LiuJiewen,... | Liu Jiewen, Qi Shuling, E de Wynter, et al. Institute of Hematology, CAMS and PUMC, Tianjin 300020, China. | 1998 | Chinese Medical Journal1998，，2： | 8 |
| 14 | Human papillomavirus 16 E6 is associated with the nuclear matrix of esophageal carcinoma cells显示文摘AIM: To explore the etiologic role of HPV infection in esophageal carcinoma, and the association of HPV-16 E6with the nuclear metrix of carcinoma cells.METHODS: Two esophageal carcinoma cell lines, EC/CUHK1 and EC/CUHK2, were tested for HPV-16 E6subgenetic fragment by polymerase chain reaction amplification of virus DNA associated nuclear matrix. RT-PCR and immunocytochemistry were also used to visualizethe expression of E6 subgene in the cells.RESULTS: The HPV-16 E6 subgenetic fragment wes found to be present in nuclear metrix-associeted DNA, E6oncoprotein localized in the nucleus where it is tightly associated with nuclear matrix after sequential extraction in EC/CUHK2 cells. It was not detected, however, in EC/CUHK1 cells.CONCLUSION: The interaction between HPV-16 E6 and nuclear matrix may contribute to the virus induced carcinogenesis in esophageal carcinoma. | S.B.Cheng E.C.Chew | 2001 | World Journal of Gastroenterology2001，7，6： | 7 |
| 15 | Immunization of mice with cells from juvenile worms of Schistosoma japonicum provides immunoprotection against schistosomiasis显示文摘To validate the protective efficacy against schistosomiasis by immunization with cells from juvenile Schistosoma japonicum in a murine model and to analyze possible factors related to protection, in this study, two independent repeated vaccination trials were performed. After three subcutaneous vaccina- tions, in trial one, in the absence of adjuvant, primary juvenile worm cells (pJCs) from S. japonicum induced remarkable average reductions in worm burden (54.3%), liver eggs per gram (LEPG) load (59.8%) as well as egg granulomas size (66.5%) compared to PBS control group (P<0.01), which were significantly higher than those elicited by fractions of juvenile worm cells (JCFs) or fractions of juvenile worms (JWFs) (P<0.05). Non-cell components of worms (WNCs) showed no significant protection. In trial two, compared to PBS control group, significant protective effect was also observed for cultured juvenile worm cells (cJCs) from S. japonicum with 58.4% worm reduction and 68.1% LEPG reduction (P<0.01). However, cultured adult worms cells (cACs) showed significantly higher worm burden (P<0.05) and egg burden (P<0.01) when compared to cJCs. Immunological analysis of trial two revealed that cJCs engendered a Th1-biased mixed Th1/Th2 type of immune response while cACs elicited a Th2-type response. Our data indicated that immunization with both primary and cultured cells from S. japonicum juvenile worms provided high immunoprotection, for which the physical character of immunogens, stage-specific parasite and the type of immune response induced might be responsible, suggesting that vaccination with whole cells from S. japonicum larvae is a promising approach to produce protec- tive immunity against schistosomiasis. | ZENG TieBing1,2, CAI LiTing1, ZENG QingRen1, YANG ShengHui1, YU Rong1, LI YanQin1, FANG HuiLong1, LI BenWen3 & ZHANG ShunKe1 1 Cell & Molecular Biological Experiment Center and Department of Parasitology, Xiangya School of Medicine, Central South Uni- versity, Changsha 410013, China 2 Institute of Pathogenic Biology, Medical College, Nanhua University, Hengyang 420001, China 3 Infectious Diseases Division, Washington University School of Medicine, Washington 63110, USA | 2007 | Science China(Life Sciences)2007，50，6： | 6 |
| 16 | Study on soil solidification based on microbiological precipitation of CaCO_3显示文摘The soil solidifying technology started booming from the 1940s and formed an integrated cross-discipline.This paper aimed to study the effect and mechanism of soil solidification by microorganisms.Soil specimens treated by different methods were designed.Then the compressive strengths of these specimens on day 7 were tested and the situ bacteria culturing technique in soil was investigated.Finally the mechanism of soil solidification by microorganisms was explored according to the decomposition rate of substrate,pH value and the SEM and XRD analysis.Experimental results showed that the soil could be solidified by filling enhancement and precipitation of microorganisms.The compressive strength increased with bacteria A but decreased with substrate B,and soil specimen got a higher strength with bacteria situ cultured.The suitable pH value for bacteria A was 8–9,and the delay of lime addition could make substrate decomposition more completely,lower the influence by the existence of substrate,and improve the strength.In XRD and SEM images,precipitation of CaCO3 could be speculated. | LU WangJie,QIAN ChunXiang & WANG RuiXing School of Materials Science & Engineering,Jiangsu Key Laboratory for Construction Materials,Southeast University,Nanjing 210089,China | 2010 | Science China(Technological Sciences)2010，53，9： | 6 |
| 17 | Cytoprotective Mechanism of the Novel Gastric Peptide BPC157 in Gastrointestinal Tract and Cultured Enteric Neurons and Glial Cells显示文摘Dear Editor,Body protection compound (BPC) 157 is a stable gastric pentadecapeptide. Predrag Sikiric’s team has carried out many investigations of its cytoprotective effects in different organs and tissues (1, 2)Their evidence indicates that BPC157 has potent cytoprotection in neural injury and gastrointestinal (GI) ulcers. Nevertheless. | Xi-Yu Wang Meihua Qu Rui Duan Dengping Shi Ling Jin Jinping Gao Jackie D.Wood Junhua Li Guo-Du Wang | 2019 | Neuroscience Bulletin2019，35，1： | 6 |
| 18 | Micronuclei and cell survival in human liver cancer cells irradiated by 25MeV/u^(40)Ar^(14+)显示文摘INTRODUCTIONHeavyionswithhighlinearenergytransfer(LET)havethefolowingadvantagesascomparedwiththeconventionalXraysandγraysth... | LI Wen Jian 1, GAO Qing Xiang 2, ZHOU Guang Ming 1 and WEI Zeng Quan 1 | 1999 | World Journal of Gastroenterology1999，5，4： | 5 |
| 19 | Inhibitory activity of polysaccharide extracts from three kinds of edible fungi on proliferation of human hepatoma SMMC-7721 cell and mouse implanted S180 tumor显示文摘AIM To determine the activities ofpolysaccharide extracts from Flammulina velutipes (Curt. ex Fr. ) Sing (FV), Lentinusedodes (LE) and Agaricus bisporus Sing (AB)on the proliferation of human hepatoma SMMC-7721 cells in vitro and on mouse implanted S-180tumors in vivo.METHODS The polysaccharide extracts were isolated from the fruit bodies of FV, LE and AB by the methods of hot-water extraction, Sevag’sremoval of proteins, ethanol precipitation,trypsin digestion and ethanol fractionalprecipitation. Human hepatoma SMMC-7721 cells were treated with 50 mg/L Polysaccharide extracts, and the mitosis index, mitochondria activity and cell proliferation were detected at different times in both control and experimental groups. The mice with S-180 implanted tumors were injected with the polysaccharide extracts at 24 mg/ kg body weight for 9 d and the tumorweight was measured on the 15th day.RESULTS The mitosis index of hepatoma cells in vitro could be significantly decreased by treatment with the polysaccharide extracts fromthe three kinds of edible fungi (P < 0 .005 ). Thecell numbers and mitochondria activity of SMMC7721 cells treated with polysaccharide extracts were lower than those in control groups (P <0.005). The inhibition rates of polysaccharide extracts against implanted S-180 tumors in mice were 52.8%, 56.6% and 51 .9% respectivelycompared with that in c0ntrol gr0ups.CONCLUSI0N The POIysaccharide extractsfrom the three kinds of edible fungi could inhibitnot only the Cultured malignant cells in vitfO butalso impIanted Sl80 tum0r i0 vivo. | Jiang SM Xiao ZM Xu ZH | 1999 | World Journal of Gastroenterology1999，5，5： | 5 |
| 20 | Nitrogen-cycling microbial community functional potential and enzyme activities in cultured biofilms with response to inorganic nitrogen availability显示文摘Biofilms mediate crucial biochemical processes in aquatic ecosystems. It was hypothesized that eutrophication may promote the growth of biofilms, resulting in larger numbers of functional genes. However, the metabolic activity and the roles of biofilms in N cycling will be affected by ambient inorganic nitrogen availability, not by the abundance of functional genes. Biofilms were cultured either with replete inorganic nitrogen(N-rep) or without exogenous inorganic nitrogen supply(N-def) in a flow incubator, and the N-cycling gene abundances(nifH, N_2 fixation; amoA, ammonia oxidation, archaea and bacteria; nirS and nirK, denitrification) and enzyme activities(nitrogenase and nitrate reductase) were analyzed. The results showed that, comparing the N-def and N-rep biofilms, the former contained lower nifH gene abundance, but higher nitrogenase activity(NA), while the latter contained higher nifH gene abundance, but lower NA. Different patterns of NA diel variations corresponded to the dynamic microbial community composition and different stages of biofilm colonization. Ammonia oxidizing bacteria(AOB), detected only in N-def biofilms, were responsible for nitrification in biofilms. N-rep biofilms contained high nirS and nirK gene abundance and high denitrification enzyme activity, but N-def biofilms contained significantly lower denitrification gene abundance and activity. In general,the strong N_2 fixation in N-def biofilms and strong denitrification in N-rep biofilms assured the balance of aquatic ecosystems. The results suggested that evaluation of the functional processes of N cycling should not only focus on genetic potential, but also on the physiological activity of biofilms. | Shuangshuang Li Chengrong Peng Taisheng Cheng Chun Wang Liangliang Guo Dunhai Li | 2019 | Journal of Environmental Sciences2019，31，2： | 4 |
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