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| 1 | A hepatoprotection study of Radix Bupleuri on acetaminophen-induced liver injury based on CYP450 inhibition显示文摘We investigated the potential hepatoprotective effect of Radix Bupleuri(RB) by inducing acute liver injury(ALI) in an animal model using acetaminophen(APAP) after pretreatment with RB aqueous extract for three consecutive days. Compared to those of the APAP group, the biochemical and histological results of the RB pretreatment group showed lower serum aspartate transaminase(AST) and alanine transaminase(ALT) levels as well as less liver damage. Pharmacokinetic study of the toxicity related marker acetaminophen-cysteine(APC) revealed a lower exposure level in rats, suggesting that RB alleviated APAP-induced liver damage by preventing glutathione(GSH) depletion. The results of cocktail approach showed significant inhibition of CYP2 E1 and CYP3 A activity. Further investigation revealed the increasing of CYP2 E1 and CYP3 A protein was significantly inhibited in pretreatment group,while no obvious effect on gene expression was found. Therefore, this study clearly demonstrates that RB exhibited significant protective action against APAP-induced acute live injury via pretreatment, and which is partly through inhibiting the increase of activity and translation of cytochrome P450 enzymes, rather than gene transcription. | WANG Yu-Xin DU Yi LIU Xia-Fei YANG Fang-Xiu WU Xiao TAN Li LU Yi-Hong ZHANG Jing-Wei ZHOU Fang WANG Guang-Ji | 2019 | Chinese Journal of Natural Medicines2019，17，7： | 18 |
| 2 | Review of natural products with hepatoprotective effects显示文摘The liver is one of the most important organs in the body,performing a fundamental role in the regulationof diverse processes,among which the metabolism,secretion,storage,and detoxification of endogenous and exogenous substances are prominent.Due to these functions,hepatic diseases continue to be among the main threats to public health,and they remain problems throughout the world.Despite enormous advances in modern medicine,there are no completely effective drugs that stimulate hepatic function,that offer complete protection of the organ,or that help to regenerate hepatic cells.Thus,it is necessary to identify pharmaceutical alternatives for the treatment of liver diseases,with the aim of these alternatives being more effective and less toxic.The use of some plants and the consumption of different fruits have played basic roles in human health care,and diverse scientific investigations have indicated that,in those plants and fruits so identified,their beneficial effects can be attributed to the presence of chemical compounds that are called phytochemicals.The present review had as its objective the collecting of data based on research conducted into some fruits(grapefruit,cranberries,and grapes)and plants[cactus pear(nopal)and cactus pear fruit,chamomile,silymarin,and spirulina],which are consumed frequently by humans and which have demonstrated hepatoprotective capacity,as well as an analysis of a resin(propolis)and some phytochemicals extracted from fruits,plants,yeasts,and algae,which have been evaluated in different models of hepatotoxicity. | Eduardo Madrigal-Santillán Eduardo Madrigal-Bujaidar Isela álvarez-González María Teresa Sumaya-Martínez José Gutiérrez-Salinas Mirandeli Bautista ángel Morales-González Manuel García-Luna y González-Rubio J Leopoldo Aguilar-Faisal José A Morales-González | 2014 | World Journal of Gastroenterology2014，20，40： | 10 |
| 3 | Hepatoprotective effects of Hovenia dulcis seeds against alcoholic liver injury and related mechanisms investigated via network pharmacology显示文摘BACKGROUND Alcoholic liver disease(ALD)is a worldwide health problem,and natural products have been shown to improve ALD due to their antioxidant activities.Some parts of Hovenia dulcis(H.dulcis),such as roots,peduncles,and stems,provide health benefits.Nevertheless,the effects and mechanisms of H.dulcis seeds on ALD have not yet been fully elucidated.AIM To determine H.dulcis antioxidant activity,evaluate its effects against ALD,and investigate the related mechanisms via network pharmacology.METHODS The antioxidant activity of H.dulcis seed was determined by both ferric-reducing antioxidant power and trolox equivalent antioxidant capacity assays.The total phenolic and flavonoid contents were determined by Folin–Ciocalteu method and aluminum chloride colorimetry,respectively,and polysaccharide was determined by phenol-sulfuric acid method.The effects of H.dulcis seeds against alcoholic liver injury were investigated in mice with water extract pretreatment for 7 days followed by alcohol administration.Moreover,the mechanisms of action were explored with network pharmacology.RESULTS The results showed that H.dulcis seeds possessed strong antioxidant activity(245.11±10.17μmol Fe2+/g by ferric-reducing antioxidant power and 284.35±23.57μmol TE/g by trolox equivalent antioxidant capacity)and contained remarkable phenols and flavonoids,as well as a few polysaccharides.H.dulcis seeds attenuated alcohol-induced oxidative liver injury,showing reduced serum alanine and aspartate aminotransferases,alkaline phosphatase,and triglyceride,elevated hepatic glutathione,increased activities of superoxide dismutase and catalase,and reduced malondialdehyde and hepatic triglyceride.The results of network pharmacology analysis indicated that kaempferol,stigmasterol,and naringenin were the main bioactive compounds in H.dulcis seeds and that modulation of oxidative stress,inflammation,gut-derived products,and apoptosis were underlying mechanisms of the protective effects of H.dulcis seeds on ALD.CONCLUSION The results of this study demonstrate that H.dulcis seeds could be a good natural antioxidant source with protective effects on oxidative diseases such as ALD. | Xiao Meng Guo-Yi Tang Cai-Ning Zhao Qing Liu Xiao-Yu Xu Shi-Yu Cao | 2020 | World Journal of Gastroenterology2020，26，24： | 7 |
| 4 | In vivo antioxidant and hepatoprotective activity of methanolic extracts of Daucus carota seeds in experimental animals显示文摘Objective:To assess the In vivo anlioxid Fanl and hepaloproleclive activity of metlianolic exlracl of Daucus carota(D.carota) seeds in experimental animals.Methods:Methanolic extracts of D.carota seeds is used for hepatoproleclion assessment.Oxidative stress were induced in rats by thioacetamide 100 nig/kg s.c.in four groups of rats(two test,standard and toxic control). Two test groups received D.carota seeds extract[DCSE) at doses of 200 mg/kg and 400 mg/kg. Standard group received silymarin(25 mg/kg) and toxic control received only thioacetamide. Control group received only vehicle.On the 8th day animals were sacrificed and liver enzyme like serum glutamic pyruvic transaminase(SGPT),serum glutamic-oxaloacetic transaminase(SCOT) and alkaline phosphatase(ALP)were estimated in blood serum and antioxidant enzyme like superoxide disnuituse(SOD),cululase(CAT),glutathione reductase(CKD),glutathione peroxidase(GPX),glutalhione-S-transferase(GST)and lipid peroxidation(LPO)were estimated in liver homogcnatc.Results:A significant decrease in SGPT,SCOT and ALP levels was observed in all drug treated groups as compared to thioacetamide group(P<0.001) and in case of antioxidant enzyme a significant(P<0.001) increase in SOD.CAT,GRD,GPX and GST was observed in all dmg treated groups as compared with thioacetamide group.But in case of LPO a significant(P <0.001) reduction was observed as compared to toxic control group.Conclusions:DCSE has contributed lo the reduction of oxidative stress and the protection of liver in experimental rals. | Kamlesh Singh Nisha Singh Anish Chandy Ashish Manigauha | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，5： | 6 |
| 5 | Hepatoprotective activity of Terminalia paniculata against paracetamol induced hepatocellular damage in Wistar albino rats显示文摘Objective:To evaluate the hepatoprotective activity of Terminalia paniculata against paracetamol induced hepatic damage in rats.Methods:The plant material was shade dried, powdered and extracted with ethanol.Liv 52 and silymarin were used as standard drugs and 2%gum acacia as a control(vehicle).Alteration in the levels of biochemical markers of hepatic damage like AST,ALT,ALP and lipid peroxides were tested,and phytochemical tests were also performed.Results:Paracetamol(2 g/kg) increased the serum levels of alanine aminotransfer (ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP) and the lipid peroxides. Treatment of Liv 52,silymarin and ethanolic extract of Terminalia paniculata(200 mg/kg) altered levels of biochemical marker and showed significant hepatoprotective activity.Ethanolic extract revealed the presence of phenolic compound and flavanoids.Our findings suggested that ethanolic bark extract of Terminalia paniculata possessed hepatoprotective activity in a dose dependent manner.Conclusions:Terminalia paniculata possesses hepatoprotective activity.It could be an effective and promising preventive agent against PCT induced hepatotoxicity. | Eesha BR Mohanbabu Amberkar V Meena Kumari K Sarath babu Vijay M Lalit M Rajput R | 2011 | Asian Pacific Journal of Tropical Medicine2011，4，6： | 5 |
| 6 | Antioxidant and hepatoprotective effects of Ajuga nipponensis extract by ultrasonic-assisted extraction显示文摘Objective: To investigate suitable condition for extraction of the active components from Ajuga nipponensis(A. nipponensis). Methods: Orthogonal experimental design was used to determine the optimal extraction parameters for ecdysterones and flavonoids. Finally, the hepatoprotective abilities of A. nipponensis extracts were evaluated by CCl_4-induced animal models. Results:Maximum yields of flavonoids(7.87±0.10) mg/g and ecdysterones(0.73±0.02) mg/g could be obtained when the extraction time was 50 min, the extraction temperature was 60 ℃, and the ratio of sample to 70%(v/v) ethanol was 1:20(w/w). The antioxidant property of A. nipponensis was correlated to the concentration of its extracts. At 5 mg/m L, A. nipponensisextract scavenged 84.8% of DPPH radical and had absorbance values of 2.43±0.04 reducing power. Upon CCl_4-induced liver injury, glutamic oxaloacetic transaminase and glutamic pyruvic transaminase decreased significantly after the mice were treated with A. nipponensis. Histological researches also explained that A. nipponensis reduced the extent of liver lesions induced by CCl_4. Conclusions: A. nipponensis exhibited potent antioxidant activity in chemical experimental models and hepatoprotective effect against CCl_4-induced liver damage. | Chang-Wei Hsieh Wen-Ching Ko Wai-Jane Ho Chao-Kai Chang Guo-Jhou Chen Jen-Chieh Tsai | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，5： | 3 |
| 7 | Phenolic compounds and hepatoprotective potential of Anastatica hierochuntica ethanolic and aqueous extracts against CCl4-induced hepatotoxicity in rats显示文摘OBJECTIVE:To investigate the effect of Anastatica hierochuntica ethanolic(KEE),aqueous(KAE)extracts,and their combination against CCl4-induced hepatotoxicity in rats.METHODS:The HPLC analysis for KEE and KAE was quantitatively carried out.Biochemical liver markers,antioxidant enzymes,and histopathological alterations were examined then total hepatoprotection potential was calculated.RESULTS:Among 9 identified phenolic compounds(PC)in KEA,sinapic acid was the highest while syringic acid was the highest among 21 identified PC in KAE.Six flavonoids were identified in KEE and two in KAE using HPLC,respectively.Oral administration of KEE,KAE,and KEE+KAE at 250 mg/kg body weight significantly reduced aspartate aminotransferase(AST),alanine aminotransferase(ALT)levels,alkaline phosphatase(ALP),total bilirubin(TBILI),and also attenuated histopathological changes.Additionally,they reduced malondialdehyde(MOD),restored reduced-glutathione(GSH),and enhanced superoxide dismutase(SOD)levels.KEE,KAE,and KEE+KAE protected the liver from CCl4-hepatotoxicity as they mainly attenuating oxidative stress.Total hepatoprotection was about 128.3%,114.5%,and 103.8%for KEE,KAE,and KEE+KAE,respectively.CONCLUSION:Biochemical observations,supplemented by histopathological examination revealed that AH affords extract-depending protection against CCl4-hepatotoxicity. | Hassan Barakat Tariq I.Almundarij | 2020 | Journal of Traditional Chinese Medicine2020，40，6： | 3 |
| 8 | Hepatoprotective effect of leaf extracts from Citrus hystrix and C.maxima against paracetamol induced liver injury in rats显示文摘The present investigation is aimed to evaluate the hepatoprotective effects of Citrus hystrix and Citrus maxima(Red and White variety)methanolic leaf extracts on paracetamol induced toxicity.Leaf extracts were given in the dose of 200 mg/kg body weight for 7 days and toxicity was induced by paracetamol(2 g/kg)on day 5.Silymarin(100 mg/kg body weight)was used as reference standard.On the 7th day animals were sacrificed and liver function markers(ALT,AST,ALP),total bilirubin and total protein in blood serums and hepatic antioxidants(SOD,CAT,GSH and GPx)in liver homogenate were estimated.The leaf extracts restored the liver function markers and hepatic antioxidants to the normal level than elevated levels noticed on paracetamol control at P<0.001.Reversal of hepatoarchitecture has also been registered.The present study shows that C.hystrix and C.maxima leaf extracts possess hepatoprotective action against paracetamol induced hepatotoxicity.©2015 Beijing Academy of Food Sciences.Production and hosting by Elsevier B.V.All rights reserved. | Arumugam Abirami Gunasekaran Nagarani Perumal Siddhuraju | 2015 | Food Science and Human Wellness2015，4，1： | 3 |
| 9 | Antioxidant and hepatoprotective effects of Boswellia ovalifoliolata bark extracts显示文摘Paracetamol(PCM) hepatotoxicity is related to reactive oxygen species(ROS) formation and excessive oxidative stress; natural antioxidant compounds have been tested as an alternative therapy. This study evaluated the hepatoprotective activity of an alcoholic extract of Boswellia ovalifoliolata(BO) bark against PCM-induced hepatotoxicity. BO extract also demonstrated antioxidant activity in vitro, as well as scavenger activity against 2, 2-diphenyl-1-picrylhydrazyl. Administration of PCM caused a significant increase in the release of transaminases, alkaline phosphatase, and lactate dehydrogenase in serum. Significant enhancement in hepatic lipid peroxidation and marked depletion in reduced glutathione were observed after parac intoxication with severe alterations in liver histology. BO treatment was able to mitigate hepatic damage induced by acute intoxication of PCM and showed a pronounced protective effect against lipid peroxidation, deviated serum enzymatic variables, and maintained glutathione status toward control. The results clearly demonstrate the hepatoprotective effect of BO against the toxicity induced by PCM. | Bandari Uma Mahesh Shweta Shrivastava Rajeswara Rao Pragada V.G.M.Naidu Ramakrishna Sistla | 2014 | Chinese Journal of Natural Medicines2014，12，9： | 3 |
| 10 | Hepatoprotective actions of melatonin:Possible mediation by melatonin receptors显示文摘Melatonin,the hormone of darkness and messenger of the photoperiod,is also well known to exhibit strong direct and indirect antioxidant properties. Melatonin has previously been demonstrated to be a powerful organ protective substance in numerous models of injury; these beneficial effects have been attributed to the hormone's intense radical scavenging capacity. The present report reviews the hepatoprotective potential of the pineal hormone in various models of oxidative stress in vivo,and summarizes the extensive literature showing that melatonin may be a suitable experimental substance to reduce liver damage after sepsis,hemorrhagic shock,ischemia/reperfusion,and in numerous models of toxic liver injury. Melatonin's influence on hepatic antioxidant enzymes and other potentially relevant pathways,such as nitric oxide signaling,hepatic cytokine and heat shock protein expression,are evaluated. Based on recent literature demonstrating the functional relevance of melatonin receptor activation for hepatic organ protection,this article finally suggests that melatonin receptors could mediate the hepatoprotective actions of melatonin therapy. | Alexander M Mathes | 2010 | World Journal of Gastroenterology2010，16，48： | 3 |
| 11 | Butein imparts free radical scavenging, anti-oxidative and proapoptotic properties in the flower extracts of Butea monosperma显示文摘The flower of Butea monosperma(Lam.)(Fabaceae)has been used in traditional Indian medicine in the treatment of many ailments including liver disorders.To understand the pharmacological basis of its beneficial effects,the extracts of dried flowers in water,methanol,butanol,ethyl acetate and acetone were evaluated for free radical scavenging and pro-apoptotic activities in cell cultures(human hepatoma Huh-7 cell line and immortalized AML-12 mouse hepatocytes).Butrin and butein-the active constituents of flower extracts-were used as reference molecules.The levels of cell injury markers like lactate dehydrogenase,glutathione and lipid peroxidation and primary antioxidant enzymes glutathione S-transferase and catalase were also measured.The aqueous and butanolic extracts exhibited better 2,2-diphenyl-1-picrylhydrazyl scavenging and cytotoxic activities in hepatoma cells than in immortalized hepatocytes.Interestingly,butein inhibited 2,2-diphenyl-1-picrylhydrazyl radical better than butrin.The aqueous and butanolic extracts were further investigated for hepatoprotection against carbon tertrachloride-induced biochemical changes and cell death.Both extracts,just as butrin and butein,significantly reversed the cellular glutathione levels and lipid peroxidation,and glutathione–S-transferase activity.Lactate dehydrogenase leakage and cell death were also prevented.However,only butein revived the catalase activity.Thus,the butein content of Butea monosperma flower extracts is important for free radical scavenging activity,apoptotic cell death and protection against oxidative injury in hepatic cells. | ANURADHA SEHRAWAT VIJAY KUMAR | 2012 | BIOCELL2012，36，2： | 2 |
| 12 | Cardio- and hepato-protective potential of methanolic extract of Syzygium cumini(L.) Skeels seeds:A diabetic rat model study显示文摘Objective: To evaluate the effect of methanolic extract of Syzygium cumini(L.) Skeels(S. cumini) seeds on the major organs in an animal model of diabetes through biochemical and histopathological studies.Methods: The methanolic extracts of S. cumini seeds(100 and 200 mg/kg body weight) were administered to alloxan-induced diabetic rats daily, with fasting blood glucose levels being measured by glucometry at one-day interval for a duration of two weeks. Biochemical assays to evaluate changes in the functions of the heart, liver,pancreas and kidney were carried out. Histopathological changes in the diabetic rat organs(pancreas, liver, heart, kidney and spleen) were also observed after the 14 days of treatment with the extracts.Results: Oral administration of methanolic extracts of S. cumini seeds(100 and 200 mg/kg body weight), with gliclazide as a positive control(25 mg/kg), showed beneficial effects including lowering blood glucose levels(P < 0.001), improved heart and liver functions, and hyperlipidemia due to diabetes. At 200 mg/kg, the extracts reversed cardiac and liver damage caused by alloxan.Conclusions: In addition to the anti-hyperglycemic activity of methanolic extracts of S. cumini seeds, the extracts demonstrates potential to minimize cardiac and hepatic complications. | Sumaiya Nahid Kishor Mazumder Zillur Rahman Saiful Islam Md. Humayun Rashid Philip Grey Kerr | 2017 | Asian Pacific Journal of Tropical Biomedicine2017，7，2： | 2 |
| 13 | Phytochemical and biological activities of Crataegus sinaica growing in Egypt显示文摘Objective:To evaluate the cardiac activity and hepatoprotection of Crataegus sinaica (C.sinaica).Methods:All the isolated compounds were isolated by open-column liquid chromatography(CC) using sephadex LH-20 as stationary phase.Elution of the column was performed with EtOH or MeOH.The phytochemical investigation of the young stem of C.sinaica for the first time together with the leaves and flowers lead to the isolation and identification of quercetin,hyperoside,vitexin-2'-0-rhamnoside,epicatechin,procyanidin B2 and procyanidins CI.Results:Rats treated with the low and high dose of C.sinaica leaves with flowers extract showed 15%and 17%reduction in the heart rate,and reduction in the STsegment by 107%and 57%;respectively.The T-amplitude was decreased by 59%of the high dose extract.On the other hand,the young steins and leaves with flowers extracts of C.sinaica on primary culture of rat hepatocytes monolayer indicated a hepatoprotection for the total extract,ethyl acetate,butanol,and chlor of orm fractions at 100μg/mL,75μg/mL,50μg/mL,and 25μg/mL; respectively.Conclusions:The results of these chemical and biological studies suggest the use of C.sinaica growing in Egypt as a preventive drug against cardiovascular and hepatic diseases. The chemical studies suggest the use of woody young stems as a newly investigated bioactive organ.The extraction of unsaturated fatty acids from the seeds of the plant would serve as a good health and nutritive product. | Alaa Tawfeek Refaat Abdelaaty Abdelaziz Shahat Nermine Ahmed Ehsan Nemat Yassin Faiza Hammouda Elsayed Abou Tabl Shams Imbabi Ismail | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，4： | 2 |
| 14 | Antioxidant and hepatoprotective activities of Dicoma anomala Sond.aqueous root extract against carbon tetrachloride-induced liver damage in Wistar rats显示文摘OBJECTIVE:To evaluates the antioxidant and hepatoprotective potentials of Dicoma anomala Sond.(Asteraceae) on body weight,feed and water intake,biochemical parameters and organ histology.METHODS:Various concentrations(1.56-25 μg/m L)were used in the in vitro assays 1,1-diphenyl-2-picryl hydrazyl(DPPH,superoxide anion,hydroxyl radicals,etc.).The effects of treatment with 125,250 and 250 mg/m L Dicoma anomala aqueous roots extract(DARE) was investigated in vivo in the CCl4-induced hepatotoxic rats during the 15 days study.RESULTS:Water extract exhibited the best activity(IC50:15.20 ± 0.03,11.70 ± 0.10,and 0.84 ± 0.05 μg/m L) in vitro in DPPH,hydroxyl and superoxide anion radicals,respectively,when compared with standards.Pre-treatment and treatment with different concentrations of DARE significantly(P < 0.05)attenuated the elevated serum activities of aspartate transaminase,alanine transaminase levels while increasing the activities of superoxide dismutase,catalase and glutathione peroxidase.The histopathological evaluations revealed extensive liver damage characterized by severe vacuolar and cytoplasmic degeneration,hepatic necrosis,and cellular infilteration in pre-treated groups while in the treated groups;such liver damages were not observed most especially at 500 mg/kg dose.CONCLUSION:The results proved the hepatoprotective potential of DARE against CCl4-induced oxidative stress.Moreover,histopathological examinations revealed better therapeutic advantage of DARE than prophylactic use. | FO Balogun AOT Ashafa | 2016 | Journal of Traditional Chinese Medicine2016，36，4： | 2 |
| 15 | Effect of methanolic extract of Tetrapleura tetraptera(Schum and Thonn)Taub leaves on hyperglycemia and indices of diabetic complications in alloxan-induced diabetic rats显示文摘Objective:To investigate the ameliorative role of Tetrapleura tetraplera(Schum and Thonn)Taub(T.tetraplera)leaf in hyperglycemia with associated conditions like oxidative stress,kidney damage and disorders in lipid metabolism.Methods:Five groups of five rats each intraperitoneally received the following treatment schedules for 7 d:untreated normal control,untreated alloxan-diabetic control,diabetic treated with glibenclamide,normal rats treated with extract(50 mg/kg)and diabetic rats treated with the extract.Evaluations were made for fasting blood sugar,body weight changes,malondialdehyde,aspartate aminotransferase,alanine aminotransferase,bilirubin,superoxide dismutase,catalase,lipid profile,packed cell volume,hemoglobin,urea and creatinine in all the rats.Results:Whereas the untreated diabetic rats showed a significant decrease(P<0.05)in packed cell volume,superoxide dismutase,catalase and high-density lipoprotein-cholesterol with a concomitant increase in the levels of malondialdehyde,fasting blood sugar,aspartate aminotransferase,alanine aminotransferase,bilirubin,urea and creatinine,administration of methanolic extract of T.tetraplera leaf or glibenclamide alleviated these altered parameters in the treated rats.Conclusions:Methanolic extract of T.tetraplera leaves possesses a potent capacity for treatment of diabetes and the accompanying complications,including oxidative stress and hyperlipidemia. | Sunday Ene-Ojo Atawodi Ojochenemi Ejeh Yakubu Mubarak Labaran Liman Dorothy Uju iliemene | 2014 | Asian Pacific Journal of Tropical Biomedicine2014，4，4： | 1 |
| 16 | Antioxidant activity and hepatoprotective effect of 10 medicinal herbs on CCl4-induced liver injury in mice显示文摘BACKGROUND Many natural products confer health benefits against diverse diseases through their antioxidant activities.Carbon tetrachloride(CCl4)is often used in animal experiments to study the effects of substances on liver injury and the related mechanisms of action,among which oxidative stress is a major pathogenic factor.AIM To compare antioxidant and hepatoprotective activities of ten herbs and identify and quantify phytochemicals for the one with strongest hepatoprotection.METHODS The antioxidant activity of ten medicinal herbs was determined by both ferricreducing antioxidant power and Trolox equivalent antioxidant capacity assays.The total phenolic and flavonoid contents were determined by Folin–Ciocalteu method and aluminum chloride colorimetry,respectively.Their effects on CCl4-induced oxidative liver injury were evaluated and compared in a mouse model by administrating each water extract(0.15 g/mL,10 mL/kg)once per day for seven consecutive days and a dose of CCl4 solution in olive oil(8%,v/v,10 mL/kg).The herb with the strongest hepatoprotective performance was analyzed for the detailed bioactive components by using high-performance liquid chromatography-electrospray ionization source-ion trap tandem mass spectrometry.RESULTS The results revealed that all tested herbs attenuated CCl4-induced oxidative liver injury;each resulted in significant decreases in levels of serum alanine transaminase,aspartate transaminase,alkaline phosphatase,and triacylglycerols.In addition,most herbs restored hepatic superoxide dismutase and catalase activities,glutathione levels,and reduced malondialdehyde levels.Sanguisorba officinalis(S.officinalis)L.,Coptis chinensis Franch.,and Pueraria lobata(Willd.)Ohwi root were the three most effective herbs,and S.officinalis L.exhibited the strongest hepatoprotective effect.Nine active components were identified in S.officinalis L.Gallic acid and(+)-catechin were quantified(7.86±0.45 mg/g and 8.19±0.57 mg/g dried weight,respectively).Furthermore,the tested herbs displayed a range of in vitro antioxidant activities proportional to their phenolic content;the strongest activities were also found for S.officinalis L.CONCLUSION This study is of value to assist the selection of more effective natural products for direct consumption and the development of nutraceuticals or therapeutics to manage oxidative stress-related diseases. | Xiao Meng Guo-Yi Tang Pin-He Liu Chan-Juan Zhao Qing Liu Hua-Bin Li | 2020 | World Journal of Gastroenterology2020，26，37： | 1 |
| 17 | Pharmacological potential of Populus nigra extract as antioxidant,anti-inflammatory,cardiovascular and hepatoprotective agent显示文摘Objective:To evaluate antioxidant,anti-inflammatory,hepatoprotective and vasorelaxant activities of Pupulus nigra flower buds ethanolic extract.Methods:Antioxidant and anti-inflammatory activities of the extract were assessed using respectively the ABTS test and the animal model of carrageenan-induced paw edema.Protection from hepatic toxicity caused by aluminum was examined by histopathologic analysis of liver sections.Vasorelaxant effect was estimated in endothelium-intact and-nibbed rings of porcine coronary arteries precontracted with high concentration of U466I9.Results:The results showed a moderate antioxidant activity(40%),but potent anti-inflammatory activity(49.9%)on carrageenan-induced mice paw edema,and also as revealed by histopathologic examination,complete protection against AlCl_3-induced hepatic toxicity.Relaxant effects of the same exlracl on vascular preparation from porcine aorta precontracted with high concentration of U466I9 were considerable at 10^(-1)g/L,and comparable(P>0.05)between endothelium-intact(67.74%,IC_(50)0.04 mg/ml.)and-rubbed(72.72%,IC_(50)=0.075 mg/ml,)aortic rings.Conclusions:The extract exerted significant anti-inflammatory,hepatoprotective and vasorelaxant activities,the latter being cndothelium-independent believed to be mediated mainly by the ability of components present in the extract to exert antioxidant properties,probably related to an inhibition of Ca^(2+)influx. | Nadjet Debbache-Benaida Dina Atmani-Kilani Valerie Barbara Schini-Keirth Nouredine Djebbli Djebbar Atmani | 2013 | Asian Pacific Journal of Tropical Biomedicine2013，3，9： | 1 |
| 18 | Hepatic endogenous defense potential of propolis after mercury intoxication显示文摘Exposure to mercuric chloride(HgCl2;5 mg kg–1 body weight;i.p.)induced oxidative stress in mice and substantially increased lipid peroxidation(LPO)and oxidized glutathione(GSSG)levels,decreased the level of reduced glu-tathione(GSH)and various antioxidant enzymes in liver and also increased the activities of liver marker enzymes in serum.Therapy with propolis extract,a resinous wax-like beehive product(200 mg kg–1 orally,after mercury administration),for 3 days inhibited LPO and the formation of GSSG and increased the level of GSH in the liver.Release of serum transaminases,alkaline phosphatase,lactate dehydrogenase and-glutamyl transpeptidase were significantly restored after propolis treatment.The activities of antioxidant enzymes,that is,superoxide dismutase,catalase,glutathione-S-transferase and glucose-6-phosphate dehydrogenase,were also concomitantly restored towards normal levels after propolis administration.These observations clearly demonstrate that propolis treatment augments antioxidant defense against mercury-induced toxicity and provide evidence that propolis has therapeutic potential as a hepatoprotective agent. | Monika BHADAURIA Sangeeta SHUKLA Ramesh MATHUR Om PAGRAWAL Sadhana SHRIVASTAVA Sonia JOHRI Deepmala JOSHI Varsha SINGH Deepak MITTAL Satendra Kumar NIRALA | 2008 | Integrative Zoology2008，3，4： | 0 |
| 19 | Is the combinational administration of doxorubicin and glutathione a reasonable proposal?显示文摘The combinational administration of antioxidants and chemotherapeutic agents during conventional cancer treatment is among one of the most controversial areas in oncology. Although the data on the combinational usage of doxorubicin (DOX) and glutathione (GSH) agents have been explored for over 20 years, the duration, administration route, and authentic rationality have not yet been fully understood yet. In the current study, we systematically investigated the pharmacokinetics (PK) and pharmacodynamics (PD) with both in vivo and in vitro models to elucidate the infiuence of GSH on the toxicity and efficacy of DOX. We first studied the cardioprotective and hepatoprotective effects of GSH in Balb/c mice, H9c2, and HL7702 cells. We showed that coadministration of exogenous GSH (5, 50, and 500 mg/kg per day, intragastric) significantly attenuated DOX-induced cardiotoxicity and hepatotoxicity by increasing intracellular GSH levels, whereas the elevated GSH concentrations did not affect the exposure of DOX in mouse heart and liver. From PK and PD perspectives, then the infiuences of GSH on the chemotherapeutic efficacy of DOX were investigated in xenografted nude mice and cancer cell models, including MCF-7, HepG2, and Caco-2 cells, which revealed that administration of exogenous GSH dose-dependently attenuated the anticancer efficacy of DOX in vivo and in vitro, although the elevated GSH levels neither infiuenced the concentration of DOX in tumors in vivo, nor the uptake of DOX in MCF-7 tumor cells in vitro. Based on the results we suggest that the combined administration of GSH and DOX should be contraindicated during chemotherapy unless DOX has caused serious hepatotoxicity and cardiotoxicity. | Bo-yu Shen Chong Chen Yang-fan Xu Jia-jia Shen Hui-min Guo Hao-feng Li Xi-nuo Li Dian Kang Yu-hao Shao Zhang-pei Zhu Xiao-xi Yin Lin Xie Guang-ji Wang Yan Liang | 2019 | Acta Pharmacologica Sinica2019，40，5： | 0 |
| 20 | Reversal of acetaminophen-generated oxidative stress and concomitant hepatotoxicity by a phytopharmaceutical product显示文摘The increasing popularity of herbal medicine and the well-established health benefits of phytochemicals have spurred the multiplicity of nutraceutical and phytopharmaceutical products.In this study,Trévo^TM,a nutraceutical and phytopharmaceutical product,was evaluated for beneficial effects in acetaminophen-induced hepatic toxicity in Wistar rats.Animals received Trévo^TM(1.5 mL/kg,3.0 mL/kg or 4.5 mL/kg)orally for 14 days.Hepatotoxicity was induced by the oral administration of acetaminophen(2 g/kg),24 h prior to sacrifice.Biochemical liver function tests,oxidative stress indicators and histoarchitectural changes were evaluated.Acetaminophen administration occasioned significant increase(P<0.05)in serum bilirubin level and activities of the aminotransferases,alkaline phosphatase,γ-glutamyltransferase and lactate dehydrogenase accompanied by a significant decrease(P<0.05)in albumin level as well as histopathological alterations in liver sections.Promotion of hepatic oxidative stress by acetaminophen was revealed by significant(P<0.05)increase in lipid peroxidation,depletion of reduced glutathione,and decrease in superoxide dismutase and catalase activities.Administration of Trévo^TM remarkably ameliorated acetaminophen-induced histopathological alterations and changes in serum and tissue biochemical markers.The protective effect of Trévo^TM(4.5 mL/kg)was at par with that of Silymarin(25 mg/kg).The present study indicates that Trévo^TM has notable salubrious effects. | Afolabi C.Akinmoladun Kehinde O.Oguntunde Lawrence O.Owolabi Omotayo B.Ilesanmi Joan O.Ogundele M.Tolulope Olaleye Afolabi A.Akindahunsi | 2017 | Food Science and Human Wellness2017，6，1： | 0 |
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