| 1 | Selective potassium uptake via biocompatible zeolite–polymer hybrid microbeads as promising binders for hyperkalemia显示文摘Patients with chronic kidney disease are at high risk of hyperkalemia that is associated with various lifethreatening complications.Treatments primarily rely on orally administered potassium binding agents,along with low curative effects and various side effects.Herein,direct serum potassium uptake was realized via zeolite–heparin-mimicking-polymer hybrid microbeads.The preparation process involved the synthesis of the heparin-mimicking polymer via the in situ cross-linking polymerization of acrylic acid and N-vinylpyrrolidone in polyethersulfone solution,the fabrication of microbeads via zeolite-mixing,electro-spraying and phase-inversion,and the subsequent aqueous-phase modifications based on ion-exchange and metal-leaching.An ultra-high(about 88%)amount of zeolite could be incorporated and well locked inside the polymer matrix.Potassium uptake capability was verified in water,normal saline and human serum,showing high selectivity and fast adsorption.The microbeads exhibited satisfying blood compatibility,negligible hemolysis ratio,prolonged clotting time,inhibited contact activation,and enhanced antifouling property toward serum proteins and cells.The proposed approach toward zeolite–heparin-mimicking-polymer hybrid microbeads provided a cheap,efficient and safe treatment protocol of hyperkalemia for the high-risk patients. | Zhoujun Wang Wei Sun Zhiwei Wei Jianxu Bao Xin Song Yupei Li Haifeng Ji Jue Zhang Chao He Baihai Su Weifeng Zhao Changsheng Zhao | 2021 | Bioactive Materials2021，6，2： | 2 |
| 3 | Detecting cell-secreted growth factors in microfluidic devices using bead-based biosensors显示文摘Microfluidic systems provide an interesting alternative to standard macroscale cell cultures due to the decrease in the number of cells and reagents as well as the improved physiology of cells confined to small volumes.However,the tools available for cell-secreted molecules inside microfluidic devices remain limited.In this paper,we describe an integrated microsystem composed of a microfluidic device and a fluorescent microbead-based assay for the detection of the hepatocyte growth factor(HGF)and the transforming growth factor(TGF)-β1 secreted by primary hepatocytes.This microfluidic system is designed to separate a cell culture chamber from sensing chambers using a permeable hydrogel barrier.Cell-secreted HGF and TGF-β1 diffuse through the hydrogel barrier into adjacent sensing channels and are detected using fluorescent microbead-based sensors.The specificity of sensing microbeads is defined by the choice of antibodies;therefore,our microfluidic culture system and sensing microbeads may be applied to a variety of cells and cell-secreted factors. | Kyung Jin Son Pantea Gheibi Gulnaz Stybayeva Ali Rahimian Alexander Revzin | 2017 | Microsystems & Nanoengineering2017，3，1： | 0 |
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