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| 1 | 快速一步法(ROSE法)提取DNA应用于RAPD-PCR扩增显示文摘对ROSE法的操作程序进行了进一步的简化,“一管一步”即可完成DNA提取全过程,系统地与CTAB和SDS法进行了比较,其提取过程明显比CTAB法、SDS法简单,提取效率分别比CTAB、SDS提高4倍和9倍,无污染物产生;与CTAB和SDs法相比分别节省费用200%和700%。提取的DNA能满足RAPD-PCR研究的需要。因此,改良后的ROSE法可能是一种适合于DNA提取自动化的方法,同时可能成为植物分子育种实验室提取DNA的常规方法。 | 方宣钧 孔巍 金芜军 | 1997 | 高技术通讯1997,7,10: | 32 |
| 2 | 苏氨酸市场现状及发展前景显示文摘 | 贾冬舒 | 2006 | 饲料广角2006,,1: | 23 |
| 3 | 语篇研究新视野——《语篇研究——跨越小句的意义》述介显示文摘 | 王振华 | 2007 | 外语教学与研究2007,39,5: | 23 |
| 4 | Exogenous ethylene influences flower opening of cut roses (Rosa hybrida) by regulating the genes encoding ethylene biosynthesis enzymes显示文摘The purpose of this paper is to investigate the differential responses of flower opening to ethylene in two cut rose cultivars, ‘Samantha’, whose opening process is promoted, and ‘Kardinal’, whose opening process is inhibited by ethylene. Ethylene production and 1- aminocyclopropane-1-carboxylate (ACC) synthase and oxidase activities were determined first. After ethylene treatment, ethylene production, ACC synthase (ACS) and ACC oxidase (ACO) activities in petals increased and peaked at the earlier stage (stage 3) in ‘Samantha’, and they were much more dramatically enhanced and peaked at the later stage (stage 4) in ‘Kardinal’ than control during vasing. cDNA fragments of three Rh-ACSs and one Rh- ACO genes were cloned and designated as Rh-ACS1, Rh-ACS2, Rh-ACS3 and Rh-ACO1 respectively. Northern blotting analysis revealed that, among three genes of ACS, ethylene-in- duced expression patterns of Rh-ACS3 gene corresponded to ACS activity and ethylene production in both cultivars. A more dramatic accumulation of Rh-ACS3 mRNA was induced by ethylene in ‘Kardinal’ than that of ‘Samantha’. As an ethylene action inhibitor, STS at concentration of 0.2 mmol/L generally inhib-ited the expression of Rh-ACSs and Rh-ACO in both cultivars, although it induced the expression of Rh-ACS3 transiently in ‘Kardinal’. Our results suggests that ‘Kardinal’ is more sensitive to ethylene than ‘Samantha’; and the changes of Rh-ACS3 expression caused by ethylene might be related to the acceleration of flower opening in ‘Samantha’ and the inhibition in ‘Kardinal’. Additional results indicated that three Rh-ACSs genes were differentially associated with flower opening and senescence as well as wounding. | MA Nan1, CAI Lei1, LU Wangjin2, TAN Hui1 & GAO Junping1 1. Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100094, China 2. Department of Horticulture, South China Agricultural University, Guangzhou 510642,China | 2005 | Science China(Life Sciences)2005,48,5: | 20 |
| 5 | 一种新的循环法汽液平衡釜——改进的Rose釜显示文摘在进行汽液平衡的研究工作中,为了获得准确可靠的汽液平衡数据,首先必须要获得能正确代表真正达到平衡的汽相和液相的样品以及能测得真正代表汽液两相达到平衡的温度。这一目的通常是通过汽液平衡釜来达到。当然。 | 周怀申 韩世钧 | 1980 | 化学工程1980,15,5: | 19 |
| 6 | UML建模工具的比较——ROSE,Visio和PowerDesigner显示文摘本文简单介绍了UML(Unified Modeling Language)的主要内容及特点,并着重讨论了支持它的三种可视化建模工具——ROSE,Visio,PowerDesigner,在分析它们各自特点的基础上提出了选择的依据。 | 吴伟敏 | 2003 | 现代计算机2003,9,6: | 17 |
| 7 | 基于UML的列控系统车载人机界面设计和实现显示文摘Graphic-User-Interface(以下简称GUI)设计是软件系统开发中的一个重要部分。针对列车运行控制系统车载人机界面的特点和功能,提出了一种适用于这种人机界面的GUI模型。利用UML(UnifiedModelingLanguage,统一建模语言)及其工具RationalRose对模型进行需求分析、总体设计和详细设计。建立了静态模型和动态模型的各种视图,描述了系统的功能需求、功能流程、类的结构与关系、视图之间的交互,提高了软件设计的质量和效率。最终利用面向对象的C++编程语言对这种人机界面进行了实现。 | 王悉 唐涛 | 2006 | 系统仿真学报2006,18,2: | 15 |
| 8 | 基于UML的GIS基础软件开发管理显示文摘本文介绍了基于UML的软件工程 ,以及将UML用于面向对象的GIS基础软件开发的方法。 | 华一新 | 2001 | 信息工程大学学报2001,2,2: | 14 |
| 9 | 基于UML的工程资料管理系统分析与设计显示文摘作为一个通用的建模语言,UML支持从需求分析、系统设计到具体实现软件开发的全过程。本文首先简要介绍了UML与基于Rose的软件系统建模方法,并以工程资料管理系统为应用背景,详细讨论了以Rose为工具建立系统的用例图、类图等静态模型和顺序图、状态图等动态模型的方法和实例。最后探讨了用UML分析设计应用软件时需要注意的问题。 | 朴春慧 赵静 李建斌 | 2008 | 微计算机信息2008,,6: | 11 |
| 10 | 基于UML的工作流管理系统建模的应用研究显示文摘首先对UML和RationalRose的基本知识以及工作流管理系统的组成和功能进行简要的论述,对于UML和Rose在工作流管理系统中的应用的优点进行了讨论,并结合改进某煤矿企业安全管理的流程的实例,论述如何利用Rose对工作流管理系统进行有效的业务建模。 | 管红杰 孟凡荣 孙晋非 | 2006 | 计算机工程与设计2006,27,1: | 11 |
| 11 | 数学日记教育功能的实践探索显示文摘作为一种新兴的数学学习方式,数学日记的教育价值已逐渐被国内外数学教育工作者所认识.如美国学者R.Borasi与B.J.Rose在对大学一年级23名学生进行了一项有关数学日记教育价值的实验研究后指出:数学写作可以表达并反思其关于数学的情感、知识、方法与观点、信念.Clarke、Waywood与Stephens对约500名学生进行了长达四年的关于数学日记的研究,得出结论:“以写学数学”对于数学交流,没有其他等值的研究方法.通过学生记日记的过程,学生能很快用他们自己个人化的语言对数学加以解释一建构意义和建立连接. | 陈玉英 | 2007 | 数学教学2007,,11: | 9 |
| 12 | Inheritance of Perpetual Blooming in Rosa chinensis ‘Old Blush'显示文摘Perpetual blooming is one of the most important biological and economical traits in modern rose, while the genetic basis underlining the control of this trait is poorly investigated. With an aim in dissecting the genetic determinism of perpetual blooming, we developed six rose populations(OB, W, F1, F2, BC1 OB and BC1W) derived from a WOB population [interspecific diploid hybridization between Rosa chinensis ‘Old Blush'(OB) and R. wichuriana ‘Basye's Thornless'(W)]. Perpetual blooming is absent both in a F1 population with 296 individuals and a BC1 W population(W as the backcross parent) with 150 individuals. However, the perpetual blooming trait showed a typical 3︰1 segregation in a backcross population BC1 OB with OB as the backcross parent. In this population with 300 individuals, 83 plants had the perpetual blooming phenotype while the other 217 featured non-perpetual blooming, indicating that the perpetual blooming trait is very likely controlled by two recessive genes in R. chinensis(rpb1 and rpb2). These genetic data suggest that the inheritance of rose perpetual blooming may be controlled by a complex mechanism. | LI Shubin ZHOU Ningning ZHOU Qing YAN Huijun JIAN Hongying WANG Qigang CHEN Min QIU Xianqin ZHANG Hao WANG Shufang LI Shufa TANG Kaixue | 2015 | Horticultural Plant Journal2015,1,2: | 9 |
| 13 | LIMS系统需求分析方法显示文摘主要介绍技术中心LIMS系统项目的需求分析方法与工具的选用,讨论了选用面向对象方法与工具的主要理由与策略以及使用多种工具与方法在需求分析中的必要性,简例说明了上述方法与工具的使用效果。 | 方海军 金红 | 2006 | 浙江冶金2006,,4: | 8 |
| 14 | UML建模技术的研究与应用显示文摘UML是一种基于面向对象的可视化建模语言。介绍了UML建模技术和UML的开发过程,并通过案例,利用Rose工具具体介绍了UML建模技术在软件开发应用中的实施过程。 | 翟亚红 杨艳霞 | 2010 | 科技创业月刊2010,23,4: | 8 |
| 15 | 基于SCA的小型化核心框架研究显示文摘针对软件无线电核心框架(CF)的软件实现相对复杂与具体应用困难等问题,在遵循软件通信架构(SCA)的基础上,通过rational rose建模软件,并采用统一建模语言(UML)对CF进行建模分析,对核心框架的部分接口进行裁剪,提出一种适合于工程应用的小型化核心框架(mini-CF),同时研究分析mini-CF的在软件无线电中的具体实现;通过与SCA核心框架的比较,得出该mini-CF可减少软件实现的复杂度,减小占用软件无线电硬件平台的资源,使软件无线电系统更易于实现与应用。 | 赵秋明 林志堂 杨莹莹 林敏 | 2010 | 计算机测量与控制2010,18,5: | 7 |
| 16 | 数字化技术在非物质文化遗产保护中的应用——以环县道情皮影为例显示文摘环县道情皮影经过百余年发展,承载和体现了当地民间社会精神、信仰、民俗、风土人情等,是一门综合性民间艺术,具有人类学、民族学、民俗学等素材的特殊研究价值.随着新媒体技术的发展,环县道情皮影正濒临失传的边缘.政府和社会各界积极寻找有效的保护方法,在诸多保护方法中,利用计算机技术和网络技术进行数字化保护是比较有效的措施之一,信息系统的开发与使用又是数字化保护中重要的方法之一.本系统就是在充分了解用户需求的基础上,利用面向对象建模工具Rational Rose设计系统功能模型与数据模型,开发出与平台无关的、可移植的B/S模式应用系统,经测试运行后基本满足用户需求. | 赵双柱 刘晓娜 张婷 | 2019 | 兰州文理学院学报(自然科学版)2019,33,4: | 6 |
| 17 | 基于UML的地理数据模型研究显示文摘分析和讨论了两种常用地理空间数据模型的缺陷与不足,探讨了使用UML进行GIS空间数据模型分析和设计的方法和步骤,并阐述了该方法的特点和优势。 | 游晓明 刘升 陈传波 | 2004 | 微电子学与计算机2004,21,5: | 6 |
| 18 | Genetic control of flowering time in woody plants: Roses as an emerging model显示文摘Genetic control of the timing of flowering in woody plants is complex and has yet to be adequately investigated due to their long life-cycle and difficulties in genetic modification.Studies in Populus,one of the best woody plant models,have revealed a highly conserved genetic network for flowering timing in annuals.However,traits like continuous flowering cannot be addressed with Populus.Roses and strawberries have relatively small,diploid genomes and feature enormous natural variation.With the development of new genetic populations and genomic tools,roses and strawberries have become good models for studying the molecular mechanisms underpinning the regulation of flowering in woody plants.Here,we review findings on the molecular and genetic factors controlling continuous flowering in roses and woodland strawberries.Natural variation at TFL1 orthologous genes in both roses and strawberries seems be the key plausible factor that regulates continuous flowering.However,recent efforts suggest that a two-recessive-loci model may explain the controlling of continuous flowering in roses.We propose that epigenetic factors,including non-coding RNAs or chromatin-related factors,might also play a role.Insights into the genetic control of flowering time variation in roses should benefit the development of new germplasm for woody crops and shed light on the molecular genetic bases for the production and maintenance of plant biodiversity. | Xue Dong Xiaodong Jiang Guoqiang Kuang Qingbo Wang Micai Zhong Dongmin Jin Jinyong Hu | 2017 | Plant Diversity2017,39,2: | 6 |
| 19 | 世界优秀月季园赏析(上)显示文摘世界上许多优秀月季园,从历史、教育或观赏的角度看,有些优秀月季园比较独特。世界月季联合会(World Federationof Rose Societies,WFRS)于1995年开始评选世界优秀月季园并给予颁奖。此评选活动每3年进行1次,到目前为止,共评出35个世界优秀月季园(GardenofExcellence)(见附表)。我国仅有1个,即深圳人民公园,始建于1983年,1986年开始栽植月季,于2009年获得该项荣誉。 | 李漫莉 刘青林 | 2011 | 中国花卉园艺2011,,10: | 6 |
| 20 | UML和ROSE工具在某损伤评估系统开发中的应用显示文摘在已有的某损伤评估系统原型基础上,着眼于开发快速评估系统时的软件质量控制问题,利用UML建模技术及ROSE工具进行系统开发和设计。文中采用UML统一建模语言进行了系统分析建模,并用ROSE工具描述了具体分析过程。 | 王润生 贾希胜 刘利 杜晓明 | 2003 | 计算机工程2003,29,18: | 6 |