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| 1 | Graft-accelerated virus-induced gene silencing facilitates functional genomics in rose flowers显示文摘Rose has emerged as a model ornamental plant for studies of flower development, senescence, and morphology, as well as the metabolism of floral fragrances and colors.Virus-induced gene silencing(VIGS) has long been used in functional genomics studies of rose by vacuum infiltration of cuttings or seedlings with an Agrobacterium suspension carrying TRV-derived vectors. However, VIGS in rose flowers remains a challenge because of its low efficiency and long time to establish silencing. Here we present a novel and rapid VIGS method that can be used to analyze gene function in rose,called ‘graft-accelerated VIGS', where axil ary sprouts are cut from the rose plant and vacuum infiltrated with Agrobacterium. The inoculated scions are then grafted back onto the plants to flower and silencing phenotypes can be observed within 5 weeks, post-infiltration. Using this new method, we successfully silenced expression of the RhDFR_1, RhA G, and RhNUDX_1 in rose flowers, and affected their color, petal number, as well as fragrance, respectively. This grafting method will facilitate high-throughput functional analysis of genes in rose flowers. Importantly, it may also be applied to other woody species that are not currently amenable to VIGS by conventional leaf or plantlet/seedling infiltration methods. | Huijun Yan Shaochuan Shi Nan Ma Xiaoqian Cao Hao Zhang Xianqin qiu Qigang Wang Hongying Jian Ningning Zhou Zhao Zhang Kaixue Tang | 2018 | Journal of Integrative Plant Biology2018，60，1： | 11 |
| 2 | Inheritance of Perpetual Blooming in Rosa chinensis ‘Old Blush'显示文摘Perpetual blooming is one of the most important biological and economical traits in modern rose, while the genetic basis underlining the control of this trait is poorly investigated. With an aim in dissecting the genetic determinism of perpetual blooming, we developed six rose populations(OB, W, F1, F2, BC1 OB and BC1W) derived from a WOB population [interspecific diploid hybridization between Rosa chinensis ‘Old Blush'(OB) and R. wichuriana ‘Basye's Thornless'(W)]. Perpetual blooming is absent both in a F1 population with 296 individuals and a BC1 W population(W as the backcross parent) with 150 individuals. However, the perpetual blooming trait showed a typical 3︰1 segregation in a backcross population BC1 OB with OB as the backcross parent. In this population with 300 individuals, 83 plants had the perpetual blooming phenotype while the other 217 featured non-perpetual blooming, indicating that the perpetual blooming trait is very likely controlled by two recessive genes in R. chinensis(rpb1 and rpb2). These genetic data suggest that the inheritance of rose perpetual blooming may be controlled by a complex mechanism. | LI Shubin ZHOU Ningning ZHOU Qing YAN Huijun JIAN Hongying WANG Qigang CHEN Min QIU Xianqin ZHANG Hao WANG Shufang LI Shufa TANG Kaixue | 2015 | Horticultural Plant Journal2015，1，2： | 9 |
| 3 | Comparison of immunohistochemistry with fluorescence in situ hybridization in determining the human epidermal growth factor receptor 2 status of breast cancer specimens: a multicenter study of 3149 Chinese patients显示文摘 | Han Xiaohong Shi Yuankai Ma Li Lyu Zheng Yang Hongying Yao Jiarui Li Jian Li Bo Qin Yan | 2014 | Chinese Medical Journal2014，，2： | 5 |
| 4 | FAK-targeting PROTAC as a chemical tool for the investigation of non-enzymatic FAK function in mice显示文摘Dear Editor,Animal models,most commonly mice,that lack a protein of interest play an important role in phenotypic and functional studies of a target gene,allowing researchers to answer various biological questions(Chaible et al,2010).At pre-sent,a variety of tools act at the DNA or RNA level to enable researchers to model gene function(and thus protein)deficiency,including nucleic acid based RNA interference(EI-bashir et al.,2001),antisense oligonucleotides(Schoch and Miller,2017),and genome editing-based CRISPR-Cas9(Doudna and Charpentier,2014)strategies.However,challenges remain. | Hongying Gao Chunwei Zheng Jian Du Yue Wu Yonghui Sun Chunsheng Han Kehkooi Kee Yu Rao | 2020 | Protein & Cell2020，11，7： | 5 |
| 5 | Post-marketing safety surveillance and reevaluation of Motherwort injection:A clinical study of 10094 cases显示文摘OBJECTIVE:To investigate the safety profiles of Motherwort injection(MI).METHODS:A multi-center,prospective and drugderived hospital intensive monitoring method was conducted to assess the safety of MI in real world applications.This study was based on a very large population after the injection was approved and marketed in China.All patients using the injection in participating hospitals were monitored to determine the incidence,pattern,severity and outcome of associated adverse events.RESULTS:The post-marketing surveillance was performed in 10 094 female patients from April to December,2015.The incidence of adverse drug reactions(ADRs) was 0.79‰(8/10 094).Among the 8 patients,the reported adverse events mainly included systemic abnormalities,such as fever,chills and eyelid edema;skin and appendages disorders,such as pruritus and rash;gastrointestinal disorders,such as nausea,abdominal distension and pain;heart rate and rhythm disorders,such as palpitation and increased heart rate.All of these ADRs were mild in severity.CONCLUSION:In this study the ADRs incidence rate of MI is very low,which supports that it is generally safe for use in obstetric and gynecological diseases.However,the total number of 8 ADRs recorded over a relatively short time span seems limited,and the low number of reports could not represent an absolute guarantee of safety. | Cao Shan Zhang Wenhao Zhao Ziwei Heng Mingli Bu Huaien Wang Hongwu Liu Xinghui Wang Zhong Cai Yan Ma Yuyan Cui Shihong Deng Jihong Ding Guifeng Ding Yajuan Dong Linhong Duan Zhentao Fan Ling Fan Yang Fu Fen He Jing Ji Shuying Jin Lin Li Hong Li Hongying Liao Tao Lu Wei Luo Xiucui Lü Zhihui Ma Fengchun Ma Dafeng Shi Tianyun Sun Juying Sun Xiaotong Teng Hong Wang Jinhua Wang Ruihua Wang Ying Wang Zhengling Xi Jie Xu Minjuan Xu Zhihong Yan Qian Yang Cuirong Yang Yimei Yin Jie Yu Jinhua Yuan Wenjun Zhang Guanli Zhang Meihua Zhao Renfeng Zhong Yonghong Zhou Jian | 2018 | Journal of Traditional Chinese Medicine2018，38，4： | 4 |
| 6 | Methods of Studying Petroleum Systems in Residual Basins-A Case Study of Mesozoic and Palaeozoic MarineBasins in Southern China显示文摘 | ZHAO Zongju, ZHU Yan, YANG Shufeng, WANG Genghai,XU Yunjun, FENG Jialiang and DENG Hongying Department of Geoscience, Zhejiang University, Hangzhou 310027, Zhejiang Hangzhou Institute of Petroleum Geology, Hangzhou 310023, Zhejiang Zhou Jian Fei Zhenbi | 2002 | Acta Geologica Sinica(English Edition)2002，76，2： | 4 |
| 7 | Nonlinear Vibrations and Stability of an Axially Moving Plate Immersed in Fluid显示文摘In this paper,the nonlinear forced vibrations and stability of an axially moving large deflection plate immersed in fluid are investigated.Based on von Karman's large deflec・tion plate theory and taking into consideration the influence of fluid-strueture interaction,axial moving and axial tension,nonlinear dynamic equations are obtained by applying D'Alembert's principle.These dynamic equations are further discretized into ordinary differential equations via the Galerkin method.The frequency-response curves of system are obtained and examined.Then numerical method is used to analyze the bifurcation behaviors of immersed plate.Results show that as the parameters vary,the system displays periodic,multi-periodic,quasi-periodic and even chaotic motion.Through the analysis on global dynamic characteristics of fluid-strueture interaction system,rich and varied nonlinear dynamic characteristics are obtained,and various ways that lead to chaotic motion of the system are further revealed. | Hongying Li Tianyi Lang Yongjun Liu Jian Li | 2019 | Acta Mechanica Solida Sinica2019，32，6： | 2 |
| 8 | Cell coalescence suppressed by crosslinking structure in polypropylene microcellular foaming显示文摘 | Zhai Wentao Wang Hongying Yu Jian | 2008 | Polymer Engineering and Science2008，48，7： | 1 |
| 9 | Foaming Behavior of Isotactic Polypropylene in Supercritical CO2 In- fluenced by Phase Morphology Via Chain Grafting 显示文摘 | Zhai Wentao Wang Hongying Yu Jian | 2008 | Poly- mer2008，49，1314： | 1 |
| 10 | Foaming Behavior of Isotactic Polypropylene in Supercritical COz Influenced by Phase Morphology via Chain Grafting显示文摘 | Zhai Wentao Wang Hongying Yu Jian | 2008 | Polymer2008，49，： | 1 |
| 11 | Cell Coalescence Suppressed by Crosslinking Structure in Polypropylene Microcellular Foaming显示文摘 | ZHAI Wentao WANG Hongying YU Jian | 2008 | Polymer Engineering & Science2008，48，7： | 1 |
| 12 | Cell coalescence suppressed by erosslinking structure in polypropylene microcellular foaming显示文摘 | Zhai Wentao Wang Hongying Yu Jian | 2008 | Polymer Engineering and Science2008，48，7： | 1 |
| 13 | Mechanism and Cellular Kinetic Studies of the Enhancement of Antioxidant Activity by Using Surface‐Functionalized Gold Nanoparticles显示文摘 | Libo Du Siqingaowa Suo Guangqing Wang Hongying Jia Ke Jian Liu Baolu Zhao Yang Liu | 2012 | Chem Eur J2012，，4： | 1 |
| 14 | Foa ming behavior of polypropylene/polystyrene blends en hanced by improved inter{acial compatibility显示文摘 | Zhai Wentao Wang Hongying Yu Jian | 2008 | Jour nal Polymer Science Part B Polymer Physics2008，46，： | 1 |
| 15 | Foaming Behavior of Polypropylene/Polystyrene Blends Enhanced by Improved Interfacial Compatibility显示文摘 | ZHAI Wentao WANG Hongying YU Jian | 2008 | Journal of Polymer Science Part B: Polymer Physics2008，46，16： | 1 |
| 16 | QSAR modeling of iNOS inhibitors based on a novel regression method 显示文摘 | Wei Long Jian Xiang Hongying Wu | 2013 | Chemometrics and Intelligent Laboratory Systems2013，128，： | 1 |
| 17 | Foaming Behavior of Isotactic Polypropylene in Supercritical CO2Influenced by Phase Morphology via Chain Grafting显示文摘 | Zhai Wentao Wang Hongying Yu Jian | 2008 | Polymer2008，49，1314： | 1 |
| 18 | Nitrogen removal enhanced by intermittent operation in a subsurface wastewater infiltration system显示文摘 | Jian Zhang Xia Huang Chaoxiang Liu Hanchang Shi Hongying Hu | 2005 | Ecological Engineering2005，，4： | 1 |
| 19 | Cell Coalescence Suppressed by Crosslinking Structure in Polypropylene Microcellular Foaming 显示文摘 | Zhai Wentao Wang Hongying Yu Jian | 2008 | Polym Eng Sci2008，48，7： | 1 |
| 20 | Foaming behavior of isotactic polypropylene in supercritical CO2 influ- enced by phase morphology via chain grafting 显示文摘 | ZHAI Wentao WANG Hongying YU Jian | 2008 | Polymer2008，49，： | 1 |
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