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| 1 | Rational Design of Hybrid Peptides: A Novel Drug Design Approach显示文摘Peptides play crucial roles in various physiological and pathological processes. Consequently, the investigation of peptide-based drugs is a highlight in the research and development of new drugs. However, natural peptides are not always ideal choices for clinical application due to their limited number and sometimes cytotoxicity to normal cells. Aiming to gain stronger or specific or novel biological effects and overcome the disadvantages of natural peptides, artificial hybrid peptides have been designed by combining the sequence of two or more different peptides with varied biological functions. Compared to natural peptides, hybrid peptides have shown better therapeutic potentials against bacteria, tumors, and metabolic diseases. In this review, design strategies, structure features and recent development of hybrid peptides are summarized;future directions for the research and development of hybrid peptide drugs are also discussed. | Chao WANG Chen YANG Yu-chen CHEN Liang MA Kun HUANG | 2019 | Current Medical Science2019，39，3： | 3 |
| 2 | Progress in coating ultraflne particles using chemical vapor deposition in fluidized bed reactors | 洪若瑜 李洪钟 | 1996 | Progress in Natural Science:Materials International1996，6，3： | 2 |
| 3 | Studies on the Preparation and the Stability of SOD Modified by Lauric Acid显示文摘Tkls paper reported that a way or Preparlng superoxlde dlsmutase (SoD) modiried by lauric acid(LA-SoD) and study on stablllty of LA-SoD. The resultS showed that LA-SoD had high speclfic actlvlty,highstablllty and high rate of recovery. The stabiliy or LA-SoD ln temperature, pH and kuman gastrlc juice wasmuch higher than that of natural SOD. Because LA-SOD ls kept for a long tlme at room temperature, it canwiden the appIled range of SoD and brlng out conslderable benirlclaI results both in society and economy. | Gui Xingfeu, Yang Ping, Qiu Yanxia, Liu Jun(Henan Medical College for Health Staff, Zhengzhou 450003,China)Zou Guolin(College of Life Sciences,Wuhan University, Wuhan 430072,China) | 1998 | Wuhan University Journal of Natural Sciences1998，3，2： | 2 |
| 4 | Coating performance on glutaraldehyde-modified wood显示文摘Scots pine(Pinus sylvestris L.) panels were modified with glutaraldehyde(GA) to various weight percent gains and subsequently coated with several commercial coatings. The drying rate and adhesion of the coatings on the modified wood were measured; the coated/modified woods were exposed outdoors to analyze how the wood modifications influence the coating deterioration. The results showed that GA modification caused an increase in the drying rate of the waterborne coatings, but had no influence on drying of tested solvent-borne coatings. GAmodification did not change the dry adhesion but reduced the wood strength in a pull-off test. Wet adhesion of waterborne coatings was improved, while that of the solvent-borne coatings tended to be somewhat reduced. During 22 months of outdoor weathering, the coated/modified samples exhibited lower moisture content than the coated/unmodified samples, but GA modification didn't contribute a substantially synergistic effect with surface coatings on resistance to weathering. | Zefang Xiao Haiou Chen Carsten Mai Holger Militz Yanjun Xie | 2019 | Journal of Forestry Research2019，30，1： | 2 |
| 5 | Deciphering nucleic acid modifications by chemical derivatization-mass spectrometry analysis显示文摘Now increasing chemical modifications are discovered on genomic DNA and RNA. Up to date, more than150 chemical modifications are identified in nucleic acids. These chemical modifications do not change the sequence of DNA and RNA, but alter their structures and biochemical properties, and eventually control or regulate the spatial and temporal expression of genes. Elucidation of the functional roles of these modifications is vital to our understanding of living organisms. However, the modifications in DNA and RNA generally have extremely low abundance in vivo. Therefore, sensitive and specific detection methods are essential to decipher the functional roles of these modifications. Chemical derivatization in combination with mass spectrometry(MS) analysis has been proved to be a promising strategy to efficiently analyze these modifications in DNA and RNA. In the last several years, many chemical derivatization-MS-based analytical methods were established for the sensitive and effective analysis of nucleic acid modifications. In this review, we summarize the recent advances for deciphering modifications in DNA and RNA by chemical derivatization-MS analysis. We hope this review can stimulate the future studies of DNA and RNA modifications. | Meng-Dan Lan Bi-Feng Yuan Yu-Qi Feng | 2019 | Chinese Chemical Letters2019，30，1： | 2 |
| 6 | Kinetics of Inactivation of Aminoacylase I During Modification of Its Thiol Groups by DPDS and PCMB显示文摘The kinetics theory of the substrate reaction during modification of enzyme activity previously described by Tsou has been applied to a study on the kinetics of the course of inactivation of aminoacylase I by DPDS and PCMB.From the results obtained we have found that the inactivation reaction of aminoacylase I by DPDS is noncomplexing inhibition,and PCMB reaction is complexing inhibition.The microscopic constants for the reaction of the inactivator with free enzyme and the enzyme-substrate complex were determined. | 王洪睿 阴勇 张英侠 王希成 周海梦 | 1993 | Science China Chemistry1993，36，1： | 2 |
| 7 | Dynamic modifications of biomacromolecules: mechanism and chemical interventions显示文摘Biological macromolecules(proteins,nucleic acids,polysaccharides,etc.)are the building blocks of life,which constantly undergo chemical modifications that are often reversible and spatial-temporally regulated.These dynamic properties of chemical modifications play fundamental roles in physiological processes as well as pathological changes of living systems.The Major Research Project(MRP)funded by the National Natural Science Foundation of China(NSFC)—'Dynamic modifications of biomacromolecules:mechanism and chemical interventions'aims to integrate cross-disciplinary approaches at the interface of chemistry,life sciences,medicine,mathematics,material science and information science with the following goals:(i)developing specific labeling techniques and detection methods for dynamic chemical modifications of biomacromolecules,(ii)analyzing the molecular mechanisms and functional relationships of dynamic chemical modifications of biomacromolecules,and(iii)exploring biomacromolecules and small molecule probes as potential drug targets and lead compounds. | Chu Wang Peng Zou Caiguang Yang Lei Liu Liang Cheng Xiaopeng He Liang Zhang Yan Zhang Hualiang Jiang Peng RChen | 2019 | Science China(Life Sciences)2019，62，11： | 2 |
| 8 | STUDIES ON SULFHYDRYL AND ALKALINE AMINO ACID RESIDUES OF FRUCTOSE-6-PHOSPHATE-2-KINASE显示文摘This Communication reports the roles of cysteine, lysine and arginine residues inchicken liver fructose-6-phosphate-2--kinase. Chemical modification of the enzyme withDTNB demonstrates that there are nine SH groups in the enzyme. Among these SH groups, atneutral pH six SHs can be titrated; in the presence of Fru6P four can be titrated; and inthe presence of ATP seven can be titrated. During the process of titration, the activity ofthe enzyme increases first and subsequently decreases gradually to the original level. NENIhas an effect similar to DTNB for the activity changes of the enzyme. On the contrary,PCMB or iodoacetic acid has a little effect on the activity of the enzyme. The pH profileof the activity shows that there is an essential ionizable group with a pKa of 9.0, mostprobably the lysyl residue, which responds to the catalytical reaction. PLP or phenylglyoxalinactivates the enzyme. For PLP inactivation, Fru6P, one of the substrates, most effectivelyprotects the enzyme. Both products Fru2,6P2 | 李林 许根俊 | 1992 | Science China Chemistry1992，35，11： | 2 |
| 9 | Biochemical properties of K_(11,48)-branched ubiquitin chains显示文摘As one of the most widely existing post-translational modification models, ubiquitination regulates diverse cellular activities. In eukaryotes, K_(11,48)-branched ubiquitin chains play key roles in cell cycle and protein quality control. However, the structural and biochemical properties of K_(11,48)-branched ubiquitin chains have not been well examined. Here we employed the synthetic linkage-and length-defined K_(11,48)-branched ubiquitin chains to examine their binding and hydrolysis properties in vitro. Quantitatively affinity determination of ubiquitin chains to the proteasome ubiquitin receptor S5 a indicated that the S5 a exhibited preference binding to K_(11,48)-branched chains over K_(11)-linked chains, but not K_(48)-conjugated chains. In addition, deubiquitination experiments were carried out and the results showed that K_(11,48)-branched chains were preferably hydrolyzed by proteasome-associated deubiquitinase Rpnll than homotypic K_(11) or K_(48)-linked chains. | Lu-Jun Liang Yanyan Si Shan Tang Dongliang Huang Zhipeng A.Wang Changlin Tian Ji-Shen Zheng | 2018 | Chinese Chemical Letters2018，29，7： | 1 |
| 10 | Chemical modification of L-asparaginase with N, O-carboxymethyl chitosan and its effects on plasma half-life and other properties显示文摘E.coli L-asparaginase,an antitumor enzyme,was chemically modified with N,O-carboxymethyl chitosan to lower its artigenicity and increase its plasma half-life.The results showed that the modified L-asparaginase has almost the same apparent Km value as that of native enzyme.The modified L-asparaginase also showed a higher protease stability against trypsin and a-chymotrypsin.After being modified,the enzyme exhibited the complete loss of antigenicity towards antiasparaginase serum.In addition,the higher the molecular weight of modifying reagents,the better the effects on reduction of antigenicity.When tested in vivo,the plasma half-life of the modified enzyme (t1/2=40 h) was over 33 times longer than that of the native enzyme (t1/2=1.2 h). | 钱国强 周菊岩 马建标 何炳林 王道宾 | 1997 | Science China Chemistry1997，40，4： | 1 |
| 11 | Structural Characterization and Chemical Modification of a Glucan from Spores of Ganoderma lucidum显示文摘A linear a-D-(13)-glucan, named PSG, was obtained from the spores of Ganoderma lucidum. The synthesis of positively and negatively charged polyelectrolytes from PSG was developed. Amine groups and carboxymethyl groups were introduced through nucleophilic substitution with 3-chloropropylamine or chloroacetic acid, respectively. Reaction conditions were varied to obtain insight into the influence of variables on the degree of substitution. | Bao, XF Fang, JN | 2001 | Chinese Chemical Letters2001，12，11： | 1 |
| 12 | Cryo-EM structure determination captures new chemical modification of protein显示文摘With the discovery of the“central dogma”of life science,the molecular mechanism of genetics and evolution of biological species has basically been established.However,the completion of the Human Genome Project at the turn of the 21st century has begun to uncover the complexity of individual lives.Although the evolution of living individuals depends mainly on the sequence of genes,its complexity and diversity cannot be explained by the“central dogma”alone.Biomolecules,including proteins,nucleic acids and polysaccharides,are the basic“building blocks”of life,providing the material basis for living processes,and are subject to dynamic chemical modifications and regulation.The dynamic chemical modifications of these biomolecules play a key role in the development of individual organisms and in the regulation of cell fate,and also play a decisive role in the onset and development of diseases. | Hualiang Jiang | 2021 | Science China(Life Sciences)2021，64，10： | 1 |
| 13 | Initial Activation of Hydrogen-Storage Alloy Electrode by Chemical Modification显示文摘Themischmetal(Mm)hydrogenstoragealoyshavesofarbeensuccesfulydevelopedasahighcapacitynegativeelectrodematerialforsecondaryb... | 卢世刚 李群 路春 刘庆国 胡蓉晖 杨汉西 | 1998 | Journal of Rare Earths1998，16，4： | 1 |
| 14 | Refolding of OPTA-labeled creatine kinase denatured by guanidinium chloride显示文摘Although refolding of fully denatured protein molecules in vitro is not a valid model of folding of the nascent peptide chain in a biologically active protein in vivo, it can provide some useful information for protein folding. Therefore, refolding of denatured proteins has been extensively studied in recent years. It has been previously reported that during the refolding of guanidine or urea denatured creatine kinase, the enzymatic activity and the na- | 汤震宇 周海梦 | 1995 | Chinese Science Bulletin1995，40，19： | 0 |
| 15 | Change of structure and properties of chemically modified flyash显示文摘Flyash is a kind of waste solid in large amounts and with a sharp increase. In 1989,discharge capacity of flyash in China was 65 million tons. It is estimated that theamount will be more than 100 million tons per annum at the turn of the 21st century.Since the utilization ratio of flyash is no more than 30% at present, treatment and | 吕瑶姣 张季爽 杨春平 彭小平 刘才群 | 1995 | Chinese Science Bulletin1995，40，18： | 0 |
| 16 | Digestion on The Nature of Lignin显示文摘Through the analysis of lignin’s s tructure,chemical properties,lignin’s modification and the application of lignin,we can better understand the nature of lignin. | 霍满堂 侯哲生 房岩朝 | 2018 | 校园英语2018，，50： | 0 |
| 17 | Hydrolysis and Cyanolysis of DTNB-Modified Creatine Kinase显示文摘The cyanolysis of DTNB-modified creatine kinase(S,S’-di-TNB-CK)has been studied.Itwas found that there exist both cyanolysis and hydrolysis at the same time under the cyanolysis condition de-scribed previously by Degani.DTNB-modified creatine kinase was rapidly hydrolyzed at pH 9.5 in the ab-sence of KCN.The hydrolysis shows biphasic kinetics as seen in the semilogarithmic pseudo-first-order rateplot.The analysis shows that about one S-TNB group/mol of DTNB-modified creatine kinase was rapidly re-leased in the fast phase of the hydrolysis reaction.The further cyanolysis of hydrolytic products showsmonophasic kinetics,and about one S-TNB group/mol of hydrolytic product was also rapidly released.Theabove results show that one of the two TNB-labeled thiol groups situated respectively at two active sites ofthe creatine kinase molecule was rapidly hydrolyzed,and the other was hydrolyzed at a very slow rate.Whenthe hydrolyzed products were cyanolyzed,the other residual TNB group was also released.These resultssuggest that the subunits of creatine kinase are asymmetrically associated.This leads to the differential envi-ronments of the two thiol groups at the active sites of two subunits.The above results also show that withthe TNB release during hydrolysis or cyanolysis,the enzymic activity was also partially recovered at the sametime.The recovery in activity is linearly related to the extent of the regeneration of reactive thiol groups.Therefore,it is suggested that the reactive thiol groups of enzyme are essential for its activity,and they arelocated in the active sites of dimeric enzyme. | 何飚 王洪睿 王希成 周海梦 | 1994 | Science China Chemistry1994，37，9： | 0 |
| 18 | 2-Chloromercuri-4-nitrophenol-modified Sites of Creatine Kinase Confirmed by FPLC显示文摘MNP-modified tryptic peptides of creatine kinase have been separated with two-dimensional electrophoresis, but the location of the SH groups of creatine kinase modified by MNP still seemed to be vague until recently (Laue, M. C. & Quiocho, F. A. , 1977). Two peptides have now been found to be the MNP-modified peptides with reversed-phase FPLC methods, and the amino acid compositions of these two peptides are in agreement with those of the two peptides around Cys-145 and Cys-253, respectively. These findings indicate that the two buried SH groups of creatine kinase have been modified by MNP. | 张平城 | 1994 | Science China Chemistry1994，37，1： | 0 |
| 19 | Chemical modification of silicene显示文摘Silicene is a two-dimensional(2D) material, which is composed of a single layer of silicon atoms with sp2–sp3mixed hybridization. The sp2–sp3mixed hybridization renders silicene excellent reactive ability, facilitating the chemical modification of silicene. It has been demonstrated that chemical modification effectively enables the tuning of the properties of silicene. We now review all kinds of chemical modification methods for silicene, including hydrogenation, halogenation,organic surface modification, oxidation, doping and formation of 2D hybrids. The effects of these chemical modification methods on the geometrical, electronic, optical, and magnetic properties of silicene are discussed. The potential applications of chemically modified silicene in a variety of fields such as electronics, optoelectronics, and magnetoelectronics are introduced. We finally envision future work on the chemical modification of silicene for further advancing the development of silicene. | 王蓉 徐明生 皮孝东 | 2015 | Chinese Physics B2015，24，8： | 0 |
| 20 | Conformational Changes at the Active Site of Creatine Kinase During Denaturation of Urea显示文摘It has been previously reported that during the denaturation of creatine kinase (ATP: creatine phosphotransferase EC 2.7.3.2) by guanidine hydrochloride, urea or SDS, inactivation occurs before noticeable conformational change of the enzyme molecule as a whole can be detected, and the inactivation rate constants of the enzyme in the above denaturant solutions are several orders of magnitude faster than those of | 阴勇 王希成 周海梦 | 1993 | Chinese Science Bulletin1993，38，23： | 0 |
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