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| 1 | Biochemical properties of K_(11,48)-branched ubiquitin chains显示文摘As one of the most widely existing post-translational modification models, ubiquitination regulates diverse cellular activities. In eukaryotes, K_(11,48)-branched ubiquitin chains play key roles in cell cycle and protein quality control. However, the structural and biochemical properties of K_(11,48)-branched ubiquitin chains have not been well examined. Here we employed the synthetic linkage-and length-defined K_(11,48)-branched ubiquitin chains to examine their binding and hydrolysis properties in vitro. Quantitatively affinity determination of ubiquitin chains to the proteasome ubiquitin receptor S5 a indicated that the S5 a exhibited preference binding to K_(11,48)-branched chains over K_(11)-linked chains, but not K_(48)-conjugated chains. In addition, deubiquitination experiments were carried out and the results showed that K_(11,48)-branched chains were preferably hydrolyzed by proteasome-associated deubiquitinase Rpnll than homotypic K_(11) or K_(48)-linked chains. | Lu-Jun Liang Yanyan Si Shan Tang Dongliang Huang Zhipeng A.Wang Changlin Tian Ji-Shen Zheng | 2018 | Chinese Chemical Letters2018，29，7： | 1 |
| 2 | Simultaneous deter- mination of Cu2+,Zn2+,Cd2+,Hg2+ and Pb2+ by using second-derivative spectrophotometry method显示文摘 | Yanyan Han Yan Li Wei Si Dong Wei Zhenxing Yao Xianpeng Zheng Bin Du Qin Wei | 2011 | Spec- trochimica Acta Part A:Molecular and Biomolecular Spectroscopy2011，79，5： | 1 |
| 3 | Semi-synthesis of disulfide-linked branched tri-ubiquitin mimics显示文摘Ubiquitin(Ub) chain isopeptide bond mimics are useful molecules for biochemical and biophysical studies. Herein, we report the semi-synthesis of the disulfide-linked K11/K48-branched tri-Ub(Ub_3^(11/48)(S-S)), the first example of an isopeptide mimic for the branched Ub chains,which have recently emerged as an interesting category of Ub modifications. Our strategy comprised the El-dependent synthesis of the Ub conjugate of aminoethanethiol, followed by disulfide formation with Ub(K11 C, K48 C). The structure of the synthetic isopeptide bond mimics was verified by the crystal structure of Ub_3^(11/48)(S-S). Deubiquitination and pulldown assays indicated that the synthetic Ub_3^(11/48)(S-S) could be hydrolyzed by linkage-specific deubiquitinases(K11-specific Cezanne and K48-specific OTUB1), and recognized by proteasomal ubiquitin receptor S5 a. | Yanyan Si Lujun Liang Shan Tang Yunkun Qi Yong Huang Lei Liu | 2018 | Science China Chemistry2018，61，4： | 1 |
| 4 | Infectivity of a genotype 4 hepatitis E virus cDNA clone by intrahepatic inoculation of laboratory rats显示文摘 | Yumin Zhu Xiaoming Yu Yuanshu Zhang Yanyan Ni Fusheng Si Ruisong Yu Shijuan Dong Yaowei Huang Zhen Li | 2013 | Veterinary Microbiology2013，，： | 1 |
| 5 | Low-dimensional non-metal catalysts:principles for regulating p-orbital-dominated reactivity显示文摘Activation of p-block elements to replace the rare and precious transition metals for renewable energy applications is highly desirable.In this review,we go over recent experimental and theoretical progress on the low-dimensional non-metal materials for clean energy production,including carbon,silicon,oxide,boron,and phosphorus-based nanostructures,with the p-block elements serving as active sites.We aim to elucidate the mechanism for triggering activity in different kinds of non-metal systems,and extract general principles for controlling the p-orbital-mediated reactivity from a theoretical point of view.The perspectives and challenges for developing high-efficiency non-metal catalysts are provided in the end. | Si Zhou Wei Pei Yanyan Zhao Xiaowei Yang Nanshu Liu Jijun Zhao | 2021 | npj Computational Materials2021，，1： | 0 |
| 6 | Integrated Transcriptome and Small RNA Sequencing Analyses Reveals Insights into the Molecular Mechanism of Seed Germination in Mung Bean显示文摘During the life cycle of a plant,seed germination is crucial.Upon ingestion of water,the dry seeds resumed energy metabolism and cellular repair.To dissect the complex mechanisms at the very beginning of seed germination,two approaches including transcriptome and small RNA sequencing were conducted during the water imbibition process of mung bean seeds compared with dry seed.The transcriptome sequencing analysis identified 10,108 differentially expressed genes(DEGs)between dry and imbibed mung bean seeds.KEGG enrichment analyses demonstrated numerous DEGs involved in hormone signaling pathways,carbohydrate,and energy metabolism.Out of the total DEGs,129 genes were investigated to involve in abscisic acid,gibberellin,and ethylene pathways,with 56 genes involved in glycolysis and TCA cycle.Through the analysis of small RNA sequencing among different samples,there were 284 miRNAs found in the imbibed and dried seeds of mung bean containing 213 known and 71 novel miRNAs.From comparison between the two types of seeds,51 miRNAs were differentially expressed.The integrated analyses of transcriptome and miRNAome data showed that 23 DEGs are the putative target genes of 8 miRNAs including miR156,miR171b-3p,miR166e-3p,miR169-1,etc.QRT-PCR was used to validate several DEGs and miRNAs.The data suggested that the seed imbibition process is regulated by a variety of DEGs and microRNAs.Meanwhile,we hope to gain a deeper insight into how mung bean seeds germinate in order to optimize seed growth. | Yanyan Pu Liwen Wang Leilei Li Yujun Si Shubin Xie Yunzhe Cong Dong Wang Yongchao Gong Rumei Tian Xue Chen Xiaoyan Zhang Min Liu Hanfeng Ding Nana Li | 2023 | Phyton-International Journal of Experimental Botany2023，92，6： | 0 |
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