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| 1 | 不同生长时期大黄鱼形态性状与体重的相关性分析显示文摘研究不同生长时期大黄鱼Pseudosciaena crocea形态性状对体重的影响效果,分别测定了1704尾13月龄和596尾20月龄闽-粤东族大黄鱼的全长(x1)、体长(x2)、体高(x3)和体重(y),计算相关系数,采用通径分析方法计算了以体重(y)为依变量、其他性状为自变量的通径系数和决定系数。结果表明,在两个不同生长时期,全长、体长、体高和体重4个性状两两之间的相关系数在0.800-0.977之间,均达到极显著水平(P<0.01);在13月龄,体高对体重的直接影响(0.522)最大,其次为体长(0.445),全长对体重的直接影响不显著(P>0.05)。在20月龄,体高对体重的直接影响(0.394)最大,其次为体长(0.328)、全长(0.271),各性状对体重的直接影响均达到极显著水平(P<0.01)。研究结果表明在不同生长时期,各形态性状对体重的直接影响是不同的,早期选种(13月龄)时要注意体高和体长的挑选,而在晚些时候(20月龄)选种,对体高、体长和全长均要考虑,才能够保证选择效果。 | 刘贤德 蔡明夷 王志勇 赵广泰 武祥伟 姚翠鸾 | 2010 | 热带海洋学报2010，29，5： | 61 |
| 2 | 饲料糖水平对大黄鱼生长和糖代谢的影响显示文摘以初始体重为(137.5±0.4)g的大黄鱼Larimichthys crocea为实验对象,在海水浮式网箱中进行为期8周的摄食生长实验,研究饲料中糖水平对其生长、饲料利用、血液生化指标和糖代谢酶活力等的影响,以确定大黄鱼的饲料糖需求量。实验饲料按等氮(粗蛋白质45%)等能(18 k J/g)设计,糖含量分别为1.75%、6.67%、13.64%、21.15%、26.69%和32.25%。结果表明随着饲料糖水平的升高,大黄鱼特定生长率(SGR)先升高后降低,当糖含量为26.69%时,SGR达最大值,显著高于糖含量为1.75%、6.67%、13.64%和32.25%处理组(P<0.05)。饲料效率(FER)和蛋白质效率(PER)均在糖含量为13.64%—21.15%时显著高于其他处理组(P<0.05)。随饲料中糖水平的升高,全鱼粗脂肪含量显著降低,在糖含量为32.25%时降至最低(10.56%),显著低于其他处理组(P<0.05)。肝体比和肝糖原含量均随饲料糖水平的升高而显著升高(P<0.05),在糖含量为32.25%时达到最大值,显著高于糖含量为1.75%和6.67%处理组(P<0.05)。随饲料糖水平的升高,血浆甘油三酯和胆固醇水平均显著降低(P<0.05),而血糖水平不受饲料糖含量的影响(P>0.05)。大黄鱼血清溶菌酶、脂蛋白脂酶和肝脂酶活性均随饲料糖水平的升高显著降低(P<0.05),而肠淀粉酶活性表现为先升高后降低,在糖含量为26.69%时,酶活力达到最大值。随饲料糖水平的升高,大黄鱼肝脏己糖激酶活性先上升后下降,在糖含量为21.15%时达到最大值,显著高于糖含量为32.25%处理组(P<0.05),而丙酮酸激酶活力在糖水平为32.25%时达到最大值,显著高于糖含量为1.75%和6.67%处理组(P<0.05)。用二次多项回归模型拟合特定生长率和饲料糖水平的关系,得到大黄鱼饲料中最适糖含量为22.7%。 | 邢淑娟 孙瑞健 马俊 韦海明 徐玮 周慧慧 张彦娇 张文兵 麦康森 | 2017 | 水生生物学报2017，41，2： | 8 |
| 3 | Transcriptome Profiling of the Abdominal Skin of Larimichthys crocea in Light Stress显示文摘Large yellow croaker(Larimichthys crocea), one of the most important marine fish species in China, can change its abdominal skin color when it is shifted from light to dark or from dark to light, providing us an opportunity of investigating the molecular responding mechanism of teleost in light stress. The gene expression profile of fish under light stress is rarely documented. In this research, the transcriptome profiles of the abdominal skin of L. crocea exposed to light or dark for 0 h, 0.5 h and 2 h were produced by next-generation sequencing(NGS). The cluster results demonstrated that stress period, rather than light intensity(e.g., light or dark), is the major influencing factor. Differently expressed genes(DEGs) were identified between 0 h and 0.5 h groups, between 0 h and 2 h groups, between 0.5 h light and 0.5 h dark, and between 2 h light and 2 h dark, respectively. The gene ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) annotation revealed that the genes relating to immunity, energy metabolism, and cytoskeletal protein binding were significantly enriched. The detailed analysis of transcriptome profiles also revealed regular gene expression trends, indicating that the elaborate gene regulation networks underlined the molecular responses of the fish to light stress. This transcriptome analysis suggested that systematic and complicated regulatory cascades were functionally activated in response to external stress, and coloration change caused by light stress was mainly attributed to the change in the density of chromatophores for L. crocea. This study also provided valuable information for skin coloration or light stress research on other marine fish species. | HAN Zhaofang LV Changhuan XIAO Shijun YE Kun ZHANG Dongling TSAI Huai Jen WANG Zhiyong | 2018 | Journal of Ocean University of China2018，17，2： | 2 |
| 4 | Subcellular Localization of Large Yellow Croaker(Larimichthys crocea) TLR21 and Expression Profiling of Its Gene in Immune Response显示文摘Toll-like receptor 21(TLR21) is a non-mammalian type TLR, and plays an important role in innate immune response in fish. In this paper, the full-length cDNA sequence of TLR21 gene was identified and characterized from large yellow croaker, Larimichthys crocea and was termed as LcTLR21. It consists of 3365 bp, including a 5'-terminal untranslated region(UTR) of 97 bp, a 3'-terminal UTR of 331 bp, and an open reading frame(ORF) of 2937 bp encoding a polypeptide of 978 amino acid residues. The deduced LcTLR21 contains a signal peptide domain at N-terminal, 12 leucine-rich repeats(LRRs) at the extracellular region, a transmembrane domain and a cytoplasmic toll-interleukin-1 receptor(TIR) domain at the C-terminal. Subcellular localization analysis revealed that the LcTLR21-GFP was constitutively expressed in cytoplasm. Tissue expression analysis indicated that LcTLR21 gene broadly expressed in most of the examined tissues, with the most predominant abundance in spleen, followed by head-kidney and liver, while the weakest expression was detected in brain. The expression level of LcTLR21 after LPS, poly I:C and Vibrio parahaemolyticus challenges was investigated in spleen, head-kidney and liver. LcTLR21 gene transcripts increased significantly in all examined tissues after the challenges, and the highest expression level was detected in liver at 24 h after poly I:C stimulation(P <0.05), suggesting that LcTLR21 might play a crucial role in fish resistance to viral and bacterial infections. | SUN Qingxue FAN Zejun YAO Cuiluan | 2018 | Journal of Ocean University of China2018，17，2： | 1 |
| 5 | Molecular Characterization, Tissue Distribution and Localization of Larimichthys crocea Kif3a and Kif3b and Expression Analysis of Their Genes During Spermiogenesis显示文摘KIF3A and KIF3B are two N-terminal motor proteins belonging to the kinesin-II superfamily that play essential roles in spermiogenesis.To understand the roles played by KIF3 A/3B during spermatogenesis of large yellow croaker Larimichthys crocea,we studied the testis characteristics at different developmental stages of L.crocea,and determined the spatiotemporal expression patterns of kif3a and kif3b during spermiogenesis.Quantitative real-time PCR(qR T-PCR)showed that the overall trends of kif3 a/3 b m RNA abundance during testis development are similar.From stage Ⅱ to stage V,kif3a/3b m RNA abundances first increased and then fell after reaching a peak at stage IV.Interestingly,the m RNA abundances of both genes at stage V were higher than those at stages Ⅱ and Ⅲ.In addition,it is worth of noting that kif3 b m RNA abundance was higher than that of kif3a at all stages.Fluorescence in situ hybridization results revealed that kif3a/3b m RNA abundance dynamics were consistent with the migration of mitochondria,the deformation of nucleus,and the formation of tail.The m RNA hybridization signals of both genes first appeared either around the nuclear periphery or on the side of the nuclei,then appeared at one side of nuclei,and finally were mainly on the tail during spermiogenesis.Our findings contributed to better understanding the molecular mechanisms of spermiogenesis in fish;and suggested that KIF3A and KIF3B may participate in the intracellular transport of mitochondria,nuclear deformation,and the formation of tail during the spermiogenesis in L.crocea. | MU Danli DU Chen FU Suyan WANG Jingqian HOU Congcong TANG Daojun ZHU Junquan | 2019 | Journal of Ocean University of China2019，18，6： | 0 |
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