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| 1 | MicroRNA in cell differentiation and development显示文摘The regulation of gene expression by microRNAs(miRNAs) is a recently discovered pattern of gene regulation in animals and plants.MiRNAs have been implicated in various aspects of animal development and cell differentiation,such as early embryonic development,neuronal development,muscle development,and lymphocyte development,by the analysis of genetic deletions of individual miRNAs in mammals.These studies show that miRNAs are key regulators in animal development and are potential causes of human diseases.Here we review some recent discoveries about the functions of miRNAs in cell differentiation and development. | SHI Yi & JIN YouXin State Key Laboratory of Molecular Biology,Shanghai Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China | 2009 | Science China(Life Sciences)2009，52，3： | 11 |
| 2 | Current status and future prospects of stem cell therapy in Alzheimer’s disease显示文摘Alzheimer’s disease is a common progressive neurodegenerative disorder, pathologically characterized by the presence of β-amyloid plaques and neurofibrillary tangles. Current treatment approaches using drugs only alleviate the symptoms without curing the disease, which is a serious issue and influences the quality of life of the patients and their caregivers. In recent years, stem cell technology has provided new insights into the treatment of neurodegenerative diseases, including Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis. Currently, the main sources of stem cells include neural stem cells, embryonic stem cells, mesenchymal stem cells, and induced pluripotent stem cells. In this review, we discuss the pathophysiology and general treatment of Alzheimer’s disease, and the current state of stem cell transplantation in the treatment of Alzheimer’s disease. We also assess future challenges in the clinical application and drug development of stem cell transplantation as a treatment for Alzheimer’s disease. | Fu-Qiang Zhang Jin-Lan Jiang Jing-Tian Zhang Han Niu Xue-Qi Fu Lin-Lin Zeng | 2020 | Neural Regeneration Research2020，15，2： | 7 |
| 3 | Insulin-producing cells are bi-potential and differentiatorsprior to proliferation in early human development显示文摘AIM: To investigate the differentiation and migration of endocrine cells to form the pancreatic islets of Langer-hans in early human development.METHODS: Embryonic pancreas of 6-14 wk gestation was observed using immunocytochemistry methods in early human development.RESULTS: Insulin and glucagon are expressed in the same epithelium cells in the pancreas. In addition, insulin-producing cells also secrete somatostatin in early human embryonic development and these insulin-producing cells also express nestin.CONCLUSION: Pancreatic duct epithelial cells that can produce insulin in early human development are precursors and still have the potential to differentiate other endocrine cells. These progenitors have differentiated before migration from primary ductal epithelium to form the pancreatic islets. | Kai-Ming Yang Wang Yong Ai-Dong Li Hui-Jun Yang | 2011 | World Journal of Diabetes2011，2，4： | 4 |
| 4 | 安康地区泥鳅胚胎发育的观察(摘要)(英文)显示文摘[目的]了解安康地区泥鳅的胚胎发育全过程。[方法]用DOM和LHRH-A2对泥鳅进行催产,将所产卵子进行人工授精后所获的胚胎24~26℃、pH值7.0~7.5的淡水中培养,进行泥鳅的胚胎发育观察,详细描述从受精卵到初孵仔鱼期的27个具体发育时期的形态特征和发育时间。[结果]泥鳅胚胎发育可划分为卵裂期、囊胚期、原肠胚期、神经胚期和器官形成期。在24~26℃、pH值7.0~7.5的淡水中,尼鳅胚肥从受精到孵化出膜历时30h45min。[结论]为泥鳅的人工繁殖和遗传育种研究提供了重要的参考。 | 张玉明 田秀娥 王永军 Yu-ming Xiu-e Yong-jun | 2010 | Agricultural Science & Technology2010，11，5： | 3 |
| 5 | 杂交鳢杭鳢1号胚胎发育过程的观察显示文摘通过尼康显微镜观察了杂交鳢杭鳢1号子一代的胚胎发育,并拍摄了其发育特征,描述了各发育时期的发育时序和形态特征.杭鳢1号的胚胎发育可分为卵裂期、囊胚期、原肠胚期、神经胚期、尾芽出现期、肌肉效应期、心跳期、出膜期等几个主要阶段.在22~25℃的水温条件下,杭鳢1号鱼卵孵化约需50 h才能破膜而出. | 姚桂桂 刘新轶 谢楠 王宇希 冯晓宇 郭水荣 | 2010 | 江西农业学报2010，22，11： | 2 |
| 6 | Acquisition of pluripotency in the chick embryo occurs during intrauterine embryonic development via a unique transcriptional network显示文摘Background: Acquisition of pluripotency by transcriptional regulatory factors is an initial developmental event that is required for regulation of cell fate and lineage specification during early embryonic development. The evolutionarily conserved core transcriptional factors regulating the pluripotency network in fishes, amphibians, and mammals have been elucidated. There are also species-specific maternally inherited transcriptional factors and their intricate transcriptional networks important in the acquisition of pluripotency. In avian species, however, the core transcriptional network that governs the acquisition of pluripotency during early embryonic development is not well understood.Results: We found that chicken NANOG(c NANOG) was expressed in the stages between the pre-ovulatory follicle and oocyte and was continuously detected in Eyal-Giladi and Kochav stage I(EGK.I) to X. However, c POUV was not expressed during fol iculogenesis, but began to be detectable between EGK.V and VI. Unexpectedly, c SOX2 could not be detected during fol iculogenesis and intrauterine embryonic development. Instead of c SOX2, c SOX3 was maternally inherited and continuously expressed during chicken intrauterine development. In addition, we found that the pluripotency-related genes such as c ENS-1, c KIT, c LIN28 A, c MYC, c PRDM14, and c SALL4 began to be dramatical y upregulated between EGK.VI and VII.Conclusion: These results suggest that chickens have a unique pluripotent circuitry since maternally inherited c NANOG and c SOX3 may play an important role in the initial acquisition of pluripotency. Moreover, the acquisition of pluripotency in chicken embryos occurs at around EGK.VI to VI I. | Jae Yong Han Hyo Gun Lee Young Hyun Park Young Sun Hwang Sang Kyung Kim Deivendran Rengaraj Byung Wook Cho Jeong Mook Lim | 2018 | Journal of Animal Science and Biotechnology2018，9，3： | 2 |
| 7 | Gap junction gene innexin3 being highly expressed in the nervous system and embryonic stage of the mud crab Scylla paramamosain显示文摘Innexin proteins are a class of transmembrane proteins existing in invertebrates and they have diverse biological functions. The innexin protein Sp-inx2 has been demonstrated to play roles in immune response and promotion of cell apoptosis in the mud crab Scylla paramamosain . One novel innexin gene, named as Sp-inx3 was characterized from S. paramamosin in this study, with an open reading frame of 1 101 bp encoding 367 amino acid residues. Multiple sequence alignment revealed that the Sp-inx3 is highly homologous with innexin3 of Cancer boredis and Homorus americanus . Quantitative real-time PCR (qPCR) and the western blotting results revealed that Sp-inx3 gene was expressed predominantly in the eyestalk, brain, and thoracic ganglion mass in both female and male crabs. The immunohistochemistry assay (IHC) also showed the widespread and intense immunoreactivity of Sp-inx3 in the brain and thoracic ganglion mass. Sp-inx3 mRNA transcription profi les exhibited signifi cantly higher expression from the embryo1 to embryo4 period and low level of expression at the prehatching period and zoea I larva period of S . paramamosain . These results indicate that the Sp-inx3 may play an important role in the nervous system and early embryonic development of S . paramamosain. | WANG Shuping CHEN Fangyi ZHANG Yaqun MA Xiaowan QIAO Kun | 2019 | Journal of Oceanology and Limnology2019，37，5： | 2 |
| 8 | Development of embryonic chick brain cells by means of photon emission显示文摘Biophoton emission, also called ultraweak photon emission, has recently become an interesting subject in biological studies. In contrast to the luciferin-luciferase reaction, low intensity emission has been found in almost every biological system. The intensity of the photon emission is very low, from a few up to some hundred photons Per cm^2 surface area per second, and the range of spectrum is from 200 to 800nm. Photon emission from | 于文斗 张锦珠 | 1995 | Chinese Science Bulletin1995，40，23： | 1 |
| 9 | Practice and thinking of the integrated curriculum of medical genetics and embryonic development显示文摘Objective To draw a conclusion from the experience on the integrated curriculum of medical genetics and embryonic development set up for the eight-year clinical medicine,and to lay the foundation for its application to the five-year clinical medicine. Methods The advantages and disadvantages of the integrated curriculumof medical genetics and embryonic development were analyzed with the comparative analysis method. And opinions of students of the eight-year clinical medicine on the teaching of the current integrated curriculum were learnt about with questionnaires. Results The current integrated curriculum of medical genetics and embryonic development has basically achieved the goal of simplifying the original teaching contents and the crossing and integration of the related discipline contents,the overall student satisfaction is high. Conclusion The integrated curriculum of medical genetics and embryonic development is helpful for the integral optimization of the medical curriculum framework,which can be expanded to the five-year clinical medicine in the future. | Ming-min GU Zhi-de DING Hong XU Lei HUANG Zhao-yuan HOU Ying-yin NI | 2015 | Medical Bulletin of Shanghai Jiaotong University2015，27，1： | 1 |
| 10 | SIRT1-dependent modulation of methylation and acetylation of histone H3 on lysine 9(H3K9)in the zygotic pronuclei improves porcine embryo development显示文摘Background: The histone code is an established epigenetic regulator of early embryonic development in mammals.The lysine residue K9 of histone H3(H3 K9) is a prime target of SIRT1, a member of NAD+-dependent histone deacetylase family of enzymes targeting both histone and non-histone substrates. At present, little is known about SIRT1-modulation of H3 K9 in zygotic pronuclei and its association with the success of preimplantation embryo development. Therefore, we evaluated the effect of SIRT1 activity on H3 K9 methylation and acetylation in porcine zygotes and the significance of H3 K9 modifications for early embryonic development.Results: Our results show that SIRT1 activators resveratrol and BML-278 increased H3 K9 methylation and suppressed H3 K9 acetylation in both the paternal and maternal pronucleus. Inversely, SIRT1 inhibitors nicotinamide and sirtinol suppressed methylation and increased acetylation of pronuclear H3 K9. Evaluation of early embryonic development confirmed positive effect of selective SIRT1 activation on blastocyst formation rate(5.2 ± 2.9% versus 32.9 ± 8.1% in vehicle control and BML-278 group, respectively; P ≤ 0.05). Stimulation of SIRT1 activity coincided with fluorometric signal intensity of ooplasmic ubiquitin ligase MDM2, a known substrate of SIRT1 and known limiting factor of epigenome remodeling.Conclusions: We conclude that SIRT1 modulates zygotic histone code, obviously through direct deacetylation and via non-histone targets resulting in increased H3 K9 me3. These changes in zygotes lead to more successful pre-implantation embryonic development and, indeed, the specific SIRT1 activation due to BML-278 is beneficial for in vitro embryo production and blastocyst achievement. | Katerina Adamkova Young-Joo Yi Jaroslav Petr Tereza Zalmanova Kristyna Hoskova Pavla Jelinkova Jiri Moravec Milena Kralickova Miriam Sutovsky Peter Sutovsky Jan Nevoral | 2018 | Journal of Animal Science and Biotechnology2018，9，2： | 1 |
| 11 | Contemporary perspective on endogenous myocardial regeneration显示文摘Considering the complex nature of the adult heart, it is no wonder that innate regenerative processes, while maintaining adequate cardiac function, fall short in myocardial jeopardy. In spite of these enchaininglimitations, cardiac rejuvenation occurs as well as restricted regeneration. In this review, the background as well as potential mechanisms of endogenous myocardial regeneration are summarized. We present and analyze the available evidence in three subsequent steps. First, we examine the experimental research data that provide insights into the mechanisms and origins of the replicating cardiac myocytes, including cell populations referred to as cardiac progenitor cells(i.e., c-kit+ cells). Second, we describe the role of clinical settings such as acute or chronic myocardial ischemia, as initiators of pathways of endogenous myocardial regeneration. Third, the hitherto conducted clinical studies that examined different approaches of initiating endogenous myocardial regeneration in failing human hearts are analyzed. In conclusion, we present the evidence in support of the notion that regaining cardiac function beyond cellular replacement of dysfunctional myocardium via initiation of innate regenerative pathways could create a new perspective and a paradigm change in heart failure therapeutics. Reinitiating cardiac morphogenesis by reintroducing developmental pathways in the adult failing heart might provide a feasible way of tissue regeneration. Based on our hypothesis 'embryonic recall', we present first supporting evidence on regenerative impulses in the myocardium, as induced by developmental processes. | Dejan Milasinovic Werner Mohl | 2015 | World Journal of Stem Cells2015，7，5： | 1 |
| 12 | Knockout of ISCA 1 causes early embryonic death in rats显示文摘Background : Iron-sulfur cluster assembly 1( ISCA 1) is an iron-sulfur(Fe/S) carrier protein that accepts Fe/S from a scaffold protein and transfers it to target proteins including the mitochondrial Fe/S containing proteins. ISCA 1 is also the newly identified causal gene for multiple mitochondrial dysfunctions syndrome( MMDS). However, our knowledge about the physiological function of ISCA 1 in vivo is currently limited. In this study, we generated an ISCA 1 knockout rat line and analyzed the embryo development. Methods : ISCA 1 knockout rats were generated by replacing the exon1 of ISCA 1 gene with the mC herry-Cre fusion gene using CRISPR-Cas9 technology. The ISCA 1 expression pattern was analyzed by fluorescence imaging using ISCA 1 promotor driven Cre and mC herry expression. The embryonic morphology was examinated by microscope and mitochondrial proteins were tested by Western blot. Results : An ISCA 1 knockout rat line was obtained, which expressed mC herry-Cre fusion protein. Both of the fluorescence images from mC herry and Cre induced mC herry in a reporter rat strain, showing that ISCA 1 expressed in most of the tissues in rats. The ISCA 1 knockout resulted in abnormal development at 8.5 days, with a significant decrease of NDUFA 9 protein and an increase of aconitase 2( ACO 2) in rat embryos. Conclusion : Deletion of ISCA 1 induced early death in rats. ISCA 1 affected the expression of key proteins in the mitochondrial respiratory chain complex, suggesting that ISCA 1 has an important influence on the respiratory complex and energy metabolism. | Xinlan Yang Dan Lu Xu Zhang Wei Chen Shan Gao Wei Dong Yuanwu Ma Lianfeng Zhang | 2019 | Animal Models and Experimental Medicine2019，2，1： | 1 |
| 13 | CDX2 is essential for human IVF early embryonic development显示文摘Human in vitro fertilization (IVF)embryos usually have developmental block at 4-8 cell stages,in which only 30%-50% percent of IVF embryos develop to blastocyst stage.This is the main cause for low efficiency and repeated treatment failure in human fertility therapy.Transcription factor CDX2 is one of the key genes in human early embryonic development,which decides the differentiation from blastomere to trophoblastic lineage.However,the relationship between the developmental block of human IVF embryos and CDX2 is poorly understood.In this study,we showed that CDX2 is crucial for human IVF embryonic development,q-PCR analysis confirmed that the level of CDX2 in developmental block embryos was dramatically decreased.Immunofluorescence analysis further demonstrated that CDX2 protein level in developmental block embryos was significantly decreased compared with that in control embryos.We also addressed whether reexpression of CDX2 could rescue developmental block defects in human IVF developmental block embryos,As expected,these developmental block defects could be partially rescued by overexpression of CDX2 protein.In conclusion,these findings suggest that CDX2 plays an important role in the process of human early embryonic development. | Wuwen Zhang Kai Li Xiufang Zhong Hua Yan Guoqing Tong | 2018 | Acta Biochimica et Biophysica Sinica2018，50，12： | 1 |
| 14 | Last but not the least: effects of laying sequence on egg color variation and embryonic development of Russet Sparrow(Passer cinnamomeus)显示文摘Background: Laying sequence has important effects on eggshell color and embryonic development in birds. Some birds can allocate resources unevenly among the eggs within a clutch, prioritizing those at the beginning of the laying sequence, in order to maximize reproductive success. The changes in egg color according to laying sequence may be an adaptation to pressure from predators or brood parasites.Methods: In this study, effects of laying sequence on egg color and embryonic heart rate in Russet Sparrows(Passer cinnamomeus) were investigated using artificial nest boxes. The eggs were divided into three groups: first to be laid, intermediate in the laying sequence, and last to be laid. We maintained the eggs in an incubator and measured embryonic heart rates.Results: Avian visual modeling showed that the background color brightness of the last eggs laid was significantly higher(whiter) than those of the other eggs. All eggs were about the same size and hatched around 13 days, indicating that laying sequence significantly affected embryonic development speed; the last eggs to be laid developed significantly faster than did the first in the clutch.Conclusions: Our study quantified the effect of laying sequence on egg color variation and proved that laying sequence has an important effect on embryonic heart rate in Russet Sparrows. | Juan Huo Tongping Su Nan Niu Canchao Yang Wei Liang | 2018 | Avian Research2018，9，3： | 0 |
| 15 | 洞庭黄鲶胚胎发育观察(英文)显示文摘[Objective] The research aimed to provide technical basis for fry rearing of Silnrus asotus in Dongting Lake.[Method] The induced spawning medicine was used in the experiment to conduct artificial induced spawning and fertilization for obtaining round green fertilized eggs.According to embryonic development,the morphological characteristics of embryo at different developmental stages were recorded detailedly through microscope.[Result] The embryonic development of Silnrus asotus in Dongting Lake was divided into 7 stages,namely, blastoderm stage,cleavage stage, blastula stage, gastrula stage,neurula stage,organogenesis stage and pre-hatching stage.After hatched for 37 h 20 min in water at 22-24 ℃, fries were come out.[Conclusion] The time sequence of Silnrus asotus in Dongting Lake was basically similar to that of other catfish,while its hatching time was shorter than that of other fish in Siluriformes. | 韩庆 晏铧 孙东方 梁轩 | 2008 | Agricultural Science & Technology2008，9，3： | 0 |
| 16 | p53 balances between tissue hierarchy and anarchy显示文摘Normal tissues are organized in a hierarchical model,whereas at the apex of these hierarchies reside stem cells (SCs) capable of self-renewal and of producing differentiated cellular progenies,leading to normal development and homeostasis.Alike,tumors are organized in a hierarchical manner,with cancer SCs residing at the apex,contributing to the development and nourishment of tumors .p53,the well-known 'guardian ofthe genome',possesses various roles in embryonic development as well as in adult SC life and serves as the ‘guardian of tissue hierarchy’.Moreover,p53 serves as a barrier for dedifferentiation and reprogramming by constraining the cells to a somatic state and preventing their conversion to SCs.On the contrary,the mutant forms of p53 that lost theirtumor suppressor activity and gain oncogenic functions serve as 'inducers oftissue anarchy' and promote cancer development.In this review,we discuss these two sides ofthe p53 token that sentence a tissue either to an ordered hierarchy and life or to anarchy and death.A better understanding of these processes may open new horizons for the development of new cancer therapies. | Gabriela Koifman Ronit Aloni-Grinstein Varda Rotter | 2019 | Journal of Molecular Cell Biology2019，11，7： | 0 |
| 17 | Single-cell sequencing: New insights into pre-implantation development, pluripotency, and germ cells显示文摘Early mammalian embryonic development is a complex,dynamic,and heterogeneous process.Although numerous efforts have been made to delineate the mechanism underlying embryonic development,many questions still remain unanswered because of the scarcity of available human materials and the minute amounts of DNA and RNA.In recently years,technical developments in high-throughput single cell sequencing have revolutionized biology and provided a novel and powerful tool to investigate the dynamics of early embryo development in a quantitative and systematic manner.Gene expression networks,epigenetic modification(including DNA methylation and chromatin accessibility),and genetic variation can be recorded simultaneously in a single cell at single base resolution.The present review highlights how the single cell sequencing has provided new insights into mammalian pre-implantation development,embryonic stem cell biology,and germline development. | 李琳 高帅 | 2019 | Science Foundation in China2019，27，2： | 0 |
| 18 | Genetic monkeys reveal a new role for a longevity protein in embryonic development显示文摘As it is involved in boosting lifespan in mammals,SIRT6 has been in the spotlight for years.In mice,SIRT6 overexpression leads to improved health and lifespan extension[1].Animals die soon after birth if SIRT-6 is deficient[2].A recent study in humans showed that SIRT6 mutation causes abnormal brain development and results in embryonic death[3].This suggested that SIRT6 may play a different role in primate development.In a recent study published in Nature,a team of Chinese Academy of Sciences scientists generated a SIRT6-deficient macaque monkey by clustered regularly interspaced short palindromic repeats-Cas9.Tlie study showed that the gene,as well as its well-known role in aging;has an additional function in embryonic development[4]. | Yuyu Niu | 2019 | National Science Review2019，6，3： | 0 |
| 19 | Preliminary study on search for genes for early embryonic development in goldfish by mRNA differential display显示文摘MATERNAL RNAs stored in eggs play an essential role during the initial stage of embryonic de-velopment in animals and they are degraded as the development proceeds. In some animals (e.g. fish and amphibian), zygotic genes begin to transcribe new mRNA only when the embryosdevelop to about midblastula stage. This degradation of maternal RNA and synthesis of | Li, SH Han, W Ma, HF | 1997 | Chinese Science Bulletin1997，42，13： | 0 |
| 20 | Does Light Exposure during Embryonic Development Affect Cognitive Behavior in a Lizard?显示文摘Light is essential for embryonic development in many ovipa rous animals including fish,amphibians,and birds.However,light may be harmful for reptile embryos developing underground where they are in complete darkness and beneath thin eggshells.Nonetheless,how embryonic light conditions affect reptile development and offspring remains largely unknown.Here we incubated eggs in dark and light conditions to determine the effects of light exposure on embryonic development and offspring visual ability,spa tial cognitive ability and growth in a lacertid lizard,Eremias argus.Our experiments demonstrated that light stimulation shortened incubation duration of eggs,but did not affect hatching success,offspring size,visual ability or survival More interestingly,light exposure during incuba tion decreased spa tial cognitive ability and post-hatching growth of offspring On the basis of nega tive effects on offspring growth rates,our study indicates that in squamate reptiles with thin eggshells,light exposure in early development has negative effects on offspring cognitive ability. | Xinghan LI Chenxu WANG Guoshuai TANG Shuran LI Liang MA Baojun SUN Yongpu ZHANG | 2020 | Asian Herpetological Research2020，11，1： | 0 |
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