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| 1 | Diagnostic value of AFP-L3 and PIVKA-Ⅱin hepatocellular carcinoma according to total-AFP显示文摘AIM:To evaluate diagnostic value ofα-fetoprotein (AFP)-L3 and prothrombin induced by vitamin K absence-Ⅱ(PIVKA-Ⅱ)in hepatocellular carcinoma(HCC). METHODS:One hundred and sixty-eight patients during routine HCC surveillance were included in this study.Of the 168 patients,90(53.6%)had HCC including newly developed HCC(n=82)or recurrent HCC after treatment(n=8).Sera were obtained during their first evaluation for HCC development and at the time of HCC diagnosis before commencing HCC treatment.HCC was diagnosed by histological examination,appropriate imaging characteristics-computed tomography or magnetic resonance imaging.Control sera were collected from 78 patients with benign liver disease(BLD),which were obtained during routine surveillance with a suspicion of HCC.AFP,AFP-L3 and PIVKA-Ⅱwere measured in the same serum by microchip capillary electrophoresis and liquid-phase binding assay on a micro-total analysis system Wako i30 auto analyzer.The performance characteristics of three tests and combined tests for the diagnosis of HCC were obtained using receiver operating characteristic curves in all populations and subgroups with AFP<20 ng/mL. RESULTS:Of 90 HCC patients,38(42.2%)patients had AFP<20 ng/mL,20(22.2%)patients had AFP 20-200 ng/mL and 32(35.6%)patients had AFP>200 ng/mL.Of the 78 BLD patients,74(94.9%)patients had AFP<20 ng/mL.After adjustment for age and HBV infection status,AFP-L3 levels were higher in HCC than in BLD among patients with low AFP levels(<20 ng/mL)(P<0.001).In a total of 168 patients,areas under the curve(AUC)for HCC were 0.879,0.887,0.801 and 0.939 for AFP,AFP-L3,PIVKA-Ⅱand the combined markers,respectively.The combined AUC for three markers showed higher value than the AUCs of individual marker(P<0.05).AFP-L3 had higher AUC value than PIVKA-Ⅱfor HCC detection in entire patients(P =0.043).With combination of AFP-L3(cut-off>5%) and PIVKA-Ⅱ(cut-off>40 AU/L),the sensitivity were 94.4%and specificity were 75.6%in all patients.In 112 patients with low AFP levels(<20 ng/mL),AUCs of AFP-L3,PIVKA-Ⅱand combine AFP-L3 and PIVKA-Ⅱtests were 0.824,0.774 and 0.939,respectively. AFP-L3 with a cut-off value of 5%showed sensitivity of 71.1%and specificity of 83.8%,and PIVKA-Ⅱwith a cut-off value of 40 AU/L had sensitivity of 57.9%and specificity of 95.9%in patients with low AFP levels. The combination of AFP-L3 and PIVKA-Ⅱincreased the sensitivity and specificity up to 92.1%and 79.7%, respectively,in low AFP group.Combined markers detected 81.8%of early stage HCC(Union for Inter-national Cancer Control stageⅠ),86.7%of small sized tumor(<2 cm)and 91.7%of single tumor of HCC in the low AFP group.In multivariate analysis,AFP-L3 was correlated with AFP and tumor size,and PIVKA-Ⅱwas correlated with laboratory tests including serum aspartate aminotransferase,total bilirubin,platelets and albumin levels.PIVKA-Ⅱhad no correlation with AFP,AFP-L3 or tumor characteristics. CONCLUSION:Combined determination of AFP-L3 and PIVKA-Ⅱcould improve the diagnostic value for HCC detection in patients with or without increased AFP levels. | Jong Young Choi Seung Won Jung Hee Yeon Kim Myungshin Kim Yonggoo Kim Dong Goo Kim Eun-Jee Oh | 2013 | World Journal of Gastroenterology2013，19，3： | 55 |
| 2 | Effect of Boschniakia rossica on expression of GSTP,p53 and p21^(ras)proteins in early stage of chemical hepatocarcinogenesis and its anti-inflammatory activities in rats显示文摘AIM To investigate the effect of Boschniakiarossica(BR)extract on expression of GST-P,p53 and p21rasproteins in early stage of chemicalhepatocarcinogenesis in rats and its anti-inflammatory activities.METHODS The expression of tumor marker-placental form glutathione S-transferase(GST-P),p53 and p21rasproteins were investigated byimmunohistochemical techniques and ABCmethod.Anti-inflammatory activities of BR werestudied by xylene and croton oil-induced mouseear edema,carrageenin,histamine and hotscald-induced rat pow edema,adjuvant-inducedrat arthritis and cotton pellet-induced mousegranuloma formation methods.RESULTS The 500 mg/kg of BR-H2O extractfractionated from BR-Methanol extract hadinhibitory effect on the formation of DEN-inducedGST-P-positive foci in rat liver(GST-P stainingwas 78% positive in DEN+AAF group vs 20%positive in DEN+AAF+BR group,P<0.05)andthe expression of mutant p53 and p21rasproteinwas lower than that of hepatic preneoplasticlesions(33% and 22% positive respectively inDEN+AAF group vs negative in DEN+AAF+BRgroup).Both CH2Cl2 and H2O extracts from BRhad anti-inflamatory effect in xylene and crotonoil-induced mouse ear edema(inhibitory rateswere 26%-29% and 35%-59%,respectively). BR-H2O extract exhibited inhibitory effect incarrageenin,histamine and hot scald-inducedhind paw edema and adjuvant-induced arthritis inrats and cotton pellet-induced granulomaformation in mice.CONCLUSION BR extract exhibited inhibitory effect on formation of preneoplastic hepatic foci in early stage of rat chemical hepato-carcinogenesis. Both CH2CI2 and H2O extracts from BR exerted anti-inflammatory effect in rats and mice. | Zong Zhu Yin Hai Ling Jin Xue Zhe Yin Tian Zhu Li Ji Shu Quan Zeng Nan Jin Institute for Cancer Research,Yanbian University College of Medicine,Yanji 133000,Jilin Province,China | 2000 | World Journal of Gastroenterology2000，6，6： | 33 |
| 3 | Protein induced by vitamin K absence or antagonist-Ⅱ versus alpha-fetoprotein in the diagnosis of hepatocellular carcinoma: A systematic review with meta-analysis显示文摘Background: As a promising biomarker of hepatocellular carcinoma(HCC), protein induced by vitamin K absence or antagonist-Ⅱ(PIVKA-Ⅱ) has been studied extensively. However, its diagnostic capability varies across HCC studies. This study aimed to compare the performance of PIVKA-Ⅱ with alpha-fetoprotein(AFP) in the diagnosis of HCC. Data sources: A systematic literature search was conducted to identify the studies from MEDLINE, Embase and Cochrane Library Databases, which were published up to December 20, 2017 to compare the diagnostic capability of PIVKA-Ⅱ and AFP for HCC. The data were pooled using random effects model. Pooled sensitivity and specificity were calculated. Summary receiver operating characteristic curve(ROC) was employed to evaluate the diagnostic accuracy of each marker. Results: Thirty-one studies were included. The pooled sensitivity(95% CI) of PIVKA-Ⅱ and AFP was 0.66(0.65–0.68) and 0.66(0.65–0.67), respectively in diagnosis of HCC; and the corresponding pooled specificity(95% CI) was 0.89(0.88–0.90) and 0.84(0.83–0.85), respectively. The area under the ROC curve(AUC) of PIVKA-Ⅱ and AFP was 0.856(0.817–0.895) and 0.770(0.728–0.811), respectively. Subgroup analysis showed that PIVKA-Ⅱ was superior to AFP in terms of the AUC for both small HCC( < 3 cm) [0.863(0.825–0.901) vs 0.717(0.658–0.776)] and large HCC( ≥ 3 cm) [0.854(0.811–0.897) vs 0.729(0.682–0.776)]; for American [0.926(0.897–0.955) vs 0.698(0.594–0.662)], European [0.772(0.743–0.801) vs 0.628(0.594–0.662)], Asian [0.838(0.812–0.864) vs 0.785(0.764–0.806)] and African [0.812(0.794–0.840) vs 0.721(0.675–0.767)] HCC patients; and for HBV-related [0.909(0.866–0.951) vs 0.714(0.673–0.755)] and mixed-etiology [0.847(0.821–0.873) vs 0.794(0.772–0.816)] HCC. Conclusion: This meta-analysis indicates that PIVKA-Ⅱ is better than AFP in terms of the accuracy for diagnosing HCC, regardless of tumor size, patient ethnic group, or HCC etiology. | Hao Xing Yi-Jie Zheng Jun Han Han Zhang Zhen-Li Li Wan-Yee Lau Feng Shen Tian Yang | 2018 | Hepatobiliary & Pancreatic Diseases International2018，17，6： | 34 |
| 4 | A NUMERICAL STUDY ON THE FORMATION AND DEVELOPMENT OF ISLAND-INDUCED CYCLONE AND ITS IMPACT ON TYPHOON STRUCTURE CHANGE AND MOTION显示文摘In this paper,the impact of the Taiwan Island topography on the structure change and motion ofTyphoon Dot(9017)during its crossing the Taiwan Island is studied with a modified version of aJMA operational regional model(Japan spectral model,JSM).A series of sensitivity experiments areconducted to detect the forming and developing mechanism of an island-induced cyclone.Results showthat lee side low pressure center plays a very important role in the formation of the induced-cyclonewhile a typhoon is approaching a large island with high mountains.The position and intensity of theinduced cyclone is sensitive to the height and location of island mountain,intensity of TC and under-lying surface topography distribution as well.A preliminary formation criterion of the island-inducedcyclone is obtained. | 孟智勇 永田雅 陈联寿 | 1996 | Acta meteorologica Sinica1996，10，4： | 24 |
| 5 | 一种基于信息检索技术的网络新闻影响力分析方法显示文摘利用信息检索领域中的相关算法,分析研究通过信息检索相关技术得到的相关信息,建立了一个网络新闻影响力模型来定量地计算一则新闻的影响力,从而估计它对社会安全产生影响的程度.在对大量实验结果的统计分析中发现,此方法可以有效地对新闻文章进行排序,发现不同新闻类型中最值得关注的新闻,其结果与人的定性判断结果具有较高的一致性. | 杨伟杰 戴汝为 崔霞 | 2009 | 软件学报2009，20，9： | 19 |
| 6 | The Instability of the Vortex Sheet along the Shear Line显示文摘The traditional Kelvin-Helmholtz notion of studying the shear instability is not suitable for the case associated with shear line with the strong wind shear in the vortex sheet. Since then. the shear instability becomes the instability of the vortex sheet. If the velocity is induced by the vortex sheet. the inequalities (l - R_r + Ri_d )> 0 and U(v.t)> U(A(t)) become the criterion of the vortex sheet instability. This criterion indicates that 1) the disposition of environment field restrains the disturbance developing along the shear line. 2) There exist multi-scale interactions in the unstable process of the shear line. The calculation of the necessary condition for the instability is also presented in this paper. | 高守亭 | 2000 | Advances in Atmospheric Sciences2000，17，4： | 19 |
| 7 | Hierarchical microstructures with high spatial frequency laser induced periodic surface structures possessing different orientations created by femtosecond laser ablation of silicon in liquids显示文摘High spatial frequency laser induced periodic surface structures(HSFLs)on silicon substrates are often developed on flat surfaces at low fluences near ablation threshold of 0.1 J/cm2,seldom on microstructures or microgrooves at relatively higher fluences above 1 J/cm^2.This work aims to enrich the variety of HSFLs-containing hierarchical microstructures,by femtosecond laser(pulse duration:457 fs,wavelength:1045 nm,and repetition rate:100 kHz)in liquids(water and acetone)at laser fluence of 1.7 J/cm^2.The period of Si-HSFLs in the range of 110–200 nm is independent of the scanning speeds(0.1,0.5,1 and 2 mm/s),line intervals(5,15 and 20μm)of scanning lines and scanning directions(perpendicular or parallel to light polarization direction).It is interestingly found that besides normal HSFLs whose orientations are perpendicular to the direction of light polarization,both clockwise or anticlockwise randomly tilted HSFLs with a maximal deviation angle of 50°as compared to those of normal HSFLSs are found on the microstructures with height gradients.Raman spectra and SEM characterization jointly clarify that surface melting and nanocapillary waves play important roles in the formation of Si-HSFLs.The fact that no HSFLs are produced by laser ablation in air indicates that moderate melting facilitated with ultrafast liquid cooling is beneficial for the formation of HSFLs by LALs.On the basis of our findings and previous reports,a synergistic formation mechanism for HSFLs at high fluence was proposed and discussed,including thermal melting with the concomitance of ultrafast cooling in liquids,transformation of the molten layers into ripples and nanotips by surface plasmon polaritons(SPP)and second-harmonic generation(SHG),and modulation of Si-HSFLs direction by both nanocapillary waves and the localized electric field coming from the excited large Si particles. | Dongshi Zhang Koji Sugioka | 2019 | Opto-Electronic Advances2019，2，3： | 17 |
| 8 | Diagnostic value of gamma-glutamyltransferase/aspartate aminotransferase ratio, protein induced by vitamin K absence or antagonist II, and alpha-fetoprotein in hepatitis B virus-related hepatocellular carcinoma显示文摘BACKGROUND Researchers have investigated the diagnostic value of protein induced by vitamin K absence or antagonist II (PIVKA-II) and alpha-fetoprotein (AFP) in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC), and obtained abundant clinical diagnostic data. However, PIVKA-II and AFP have unsatisfactory specificity and sensitivity in the diagnosis of early-stage HBV-related HCC. Gamma-glutamyltransferase (γ-GT) and aspartate aminotransferase (AST) are common biomarkers for evaluating liver function, and we hypothesized that the γ-GT/AST ratio in combination with PIVKA-II and AFP would improve the diagnosis of early-stage HBV-related HCC. AIM To evaluate the diagnostic value of γ-GT/AST ratio alone or in combination with PIVKA-II and AFP in HBV-related HCC. METHODS Serum levels of γ-GT, AST, PIVKA-II, and AFP were detected and analysed in 176 patients with HBV-related HCC and in 359 patients with chronic hepatitis B. According to tumour size and serum level of HBV DNA, HBV-related HCC patients were divided into the following categories: Early-stage HCC patients, HCC patients, HBV DNA positive (HBV DNA+) HCC patients, and HBV DNA negative (HBV DNA-) HCC patients. Receiver-operating characteristic (ROC) curves were used to analyse and compare the diagnostic value of the single and combined detection of various biomarkers in different types of HBV-related HCC. RESULTS Tumour size was positively correlated with serum levels of PIVKA-II and AFP in HCC patients (r = 0.529, aP < 0.001 and r = 0.270, bP < 0.001, respectively), but there was no correlation between tumour size and the γ-GT/AST ratio (r = 0.073, P = 0.336). The areas under the receiver-operating characteristic curves (AUROCs) of the γ-GT/AST ratio in early-stage HCC patients, HBV DNA+ HCC patients and HBV DNA- HCC patients were not significantly different from that in the total HCC patients (0.754, 0.802, and 0.705 vs 0.779, respectively;P > 0.05). When PIVKA-II was combined with the γ-GT/AST ratio in the diagnosis of earlystage HCC, HCC, and HBV DNA+ HCC, the AUROCs of PIVKA-II increased, with values of 0.857 vs 0.835, 0.925 vs 0.913, and 0.958 vs 0.954, respectively. When AFP was combined with the γ-GT/AST ratio in the diagnosis of early-stage HCC, HCC, HBV DNA+ HCC, and HBV DNA- HCC, the AUROCs of AFP increased, with values of 0.757 vs 0.621, 0.837 vs 0.744, 0.868 vs 0.757, and 0.840 vs 0.828, respectively. CONCLUSION The γ-GT/AST ratio may be better than PIVKA-II and AFP in the diagnosis of early-stage HBV-related HCC, and its combination with PIVKA-II and AFP can improve the diagnostic value for HBV-related HCC. | Qiang Wang Qi Chen Xia Zhang Xiao-Lan Lu Qin Du Tao Zhu Guo-Yuan Zhang Dong-Sheng Wang Qu-Ming Fan | 2019 | World Journal of Gastroenterology2019，25，36： | 17 |
| 9 | The Practice of Forward Prospecting of Adverse Geology Applied to Hard Rock TBM Tunnel Construction: The Case of the Songhua River Water Conveyance Project in the Middle of Jilin Province显示文摘 | Shucai Li Lichao Nie Bin Liu | 2018 | Engineering2018，4，1： | 15 |
| 10 | 超级贝氏体组织中残余奥氏体的TRIP效应研究显示文摘针对超级贝氏体组织中残余奥氏体的应力诱发相变及对钢的力学性能影响,设计试验钢60Mn2Si Cr,经900℃完全奥氏体化保温30 min,在260℃盐浴炉中等温处理12 h后,将试样在疲劳试验机上进行加载试验;利用扫描电镜(Scanning electron microscope,SEM)、透射电镜(Transmission electron microscope,TEM)、X射线衍射仪(X-ray diffracmeter,XRD)和拉伸试验机等仪器设备对加载前后的样品,分别进行显微组织形貌观察和相组成的定性定量检测分析以及力学性能测试;结果显示,等温处理后样品的显微组织为超级贝氏体(贝氏体铁素体BF+残余奥氏体AR),残余奥氏体体积分数为9.4%,其含碳量为1.296%;经加载后试样显微组织中残余奥氏体体积分数下降至6.1%,含碳量达1.439%;钢的强塑积提高近20%。这说明等温处理获得超级贝氏体组织的钢,经施加载荷给予应力作用,显微组织中残余奥氏体发生转变,即超级贝氏体组织中的残余奥氏体能够通过相变诱发塑性(Transformation induced plasticity,TRIP)效应的产生,提高钢的力学性能。 | 吴化 姜颖 尤申申 韩英 刘云旭 | 2014 | 机械工程学报2014，50，22： | 14 |
| 11 | Expanding etiology of progressive familial intrahepatic cholestasis显示文摘BACKGROUND Progressive familial intrahepatic cholestasis(PFIC)refers to a disparate group of autosomal recessive disorders that are linked by the inability to appropriately form and excrete bile from hepatocytes,resulting in a hepatocellular form of cholestasis.While the diagnosis of such disorders had historically been based on pattern recognition of unremitting cholestasis without other identified molecular or anatomic cause,recent scientific advancements have uncovered multiple specific responsible proteins.The variety of identified defects has resulted in an ever-broadening phenotypic spectrum,ranging from traditional benign recurrent jaundice to progressive cholestasis and end-stage liver disease.AIM To review current data on defects in bile acid homeostasis,explore the expanding knowledge base of genetic based diseases in this field,and report disease characteristics and management.METHODS We conducted a systemic review according to PRISMA guidelines.We performed a Medline/PubMed search in February-March 2019 for relevant articles relating to the understanding,diagnosis,and management of bile acid homeostasis with a focus on the family of diseases collectively known as PFIC.English only articles were accessed in full.The manual search included references of retrieved articles.We extracted data on disease characteristics,associations with other diseases,and treatment.Data was summarized and presented in text,figure,and table format.RESULTS Genetic-based liver disease resulting in the inability to properly form and secrete bile constitute an important cause of morbidity and mortality in children and increasingly in adults.A growing number of PFIC have been described based on an expanded understanding of biliary transport mechanism defects and the development of a common phenotype.CONCLUSION We present a summary of current advances made in a number of areas relevant to both the classically described FIC1(ATP8B1),BSEP(ABCB11),and MDR3(ABCB4)transporter deficiencies,as well as more recently described gene mutations--TJP2(TJP2),FXR(NR1H4),MYO5B(MYO5B),and others which expand the etiology and understanding of PFIC-related cholestatic diseases and bile transport. | Sarah AF Henkel Judy H Squires Mary Ayers Armando Ganoza Patrick Mckiernan James E Squires | 2019 | World Journal of Hepatology2019，11，5： | 13 |
| 12 | Human parthenogenetic embryonic stem cells:one potential resource for cell therapy显示文摘Pluripotent stem cells derived from somatic cells through such processes as nuclear transfer or induced pluripotent stem(iPS) cells present an important model for biomedical research and provide potential resources for cell replacement therapies.However,the overall efficiency of the conversional nuclear transfer is very low and the safety issue remains a major concern for iPS cells.Embryonic stem cells(ESCs) generated from parthenogenetic embryos are one attractive alternative as a source of histocompatible cells and tissues for cell therapy.Recent studies on human parthenogenetic embryonic stem cells(hPG ESCs) have revealed that these ESCs are very similar to the hESCs derived from IVF or in vivo produced blastocysts in gene expression and other characteristics,but full differentiation and development potential of these hPG ESCs have to be further investigated before clinical research and therapeutic interventions.To generate various pluripotent stem cells,diverse reprogramming techniques and approaches will be developed and integrated.This may help elucidate the fundamental mechanisms underlying reprogramming and stem cell biology,and ultimately benefit cell therapy and regenerative medicine. | HAO Jie1,2,ZHU WanWan1,SHENG Chao1,YU Yang1,ZHOU Qi1 1 State Key Laboratory of Reproductive Biology,Institute of Zoology,Beijing 100101,China 2 Graduate School,Chinese Academy of Sciences,Beijing 100049,China | 2009 | Science China(Life Sciences)2009，52，7： | 12 |
| 13 | 云南红豆杉愈伤组织培养及其生产紫杉醇的研究显示文摘云南红豆杉愈伤组织培养及其生产紫杉醇的研究甘烦远彭丽萍郑光植(中国科学院昆明植物研究所昆明650204)从红豆杉科红豆杉属植物如短叶红豆杉(Taxusbrevifolia)等的树皮或枝叶提取到的紫杉醇(Taxol)是一种具有强抗癌活性的二萜烯类化合... | 甘烦远 彭丽萍 郑光植 | 1996 | 生物工程学报1996，12，S1： | 12 |
| 14 | Current progress and prospects of induced pluripotent stem cells显示文摘Induced pluripotent stem(iPS) cells are derived from somatic cells by ectopic expression of few transcription factors.Like embryonic stem(ES) cells,iPS cells are able to self-renew indefinitely and to differentiate into all types of cells in the body.iPS cells hold great promise for regenerative medicine,because iPS cells circumvent not only immunological rejection but also ethical issues.Since the first report on the derivation of iPS cells in 2006,many laboratories all over the world started research on iPS cells and have made significant progress.This paper reviews recent progress in iPS cell research,including the methods to generate iPS cells,the molecular mechanism of reprogramming in the formation of iPS cells,and the potential applications of iPS cells in cell replacement therapy.Current problems that need to be addressed and the prospects for iPS research are also discussed. | CHEN LingYi & Liu Lin Key Laboratory of Bioactive Materials of Ministry of Education,College of Life Sciences,Nankai University,Tianjin 300071,China | 2009 | Science China(Life Sciences)2009，52，7： | 11 |
| 15 | Drug and herb induced liver injury: Council for International Organizations of Medical Sciences scale for causality assessment显示文摘Causality assessment of suspected drug induced liver injury(DILI) and herb induced liver injury(HILI) is hampered by the lack of a standardized approach to be used by attending physicians and at various subsequent evaluating levels. The aim of this review was to analyze the suitability of the liver specific Council for International Organizations of Medical Sciences(CIOMS) scale as a standard tool for causality assessment in DILI and HILI cases. PubMed database was searched for the following terms: drug induced liver injury; herb induced liver injury; DILI causality assessment; and HILI causality assessment. The strength of the CIOMS lies in its potential as a standardized scale for DILI and HILI causality assessment. Other advantages include its liver specificity and its validation for hepatotoxicity with excellent sensitivity, specificity and predictive validity, based on cases with a positive reexposure test. This scale allows prospective collection of all relevant data required for a valid causality assessment. It does not require expert knowledge in hepatotoxicity and its results may subsequently be refined. Weaknesses of the CIOMS scale include the limited exclusion of alternative causes and qualitatively graded risk factors. In conclusion, CIOMS appears to be suitable as a standard scale for attending physicians, regulatory agencies, expert panels and other scientists to provide a standardized, reproducible causality assessment in suspected DILI and HILI cases, applicable primarily at all assessing levels involved. 2014 Baishideng Publishing Group Co., Limited. All | Rolf Teschke Albrecht Wolff Christian Frenzel Alexander Schwarzenboeck Johannes Schulze Axel Eickhoff | 2014 | World Journal of Hepatology2014，6，1： | 11 |
| 16 | CEL Ⅰ酶的粗纯化及活性分析显示文摘TILLING(targeting induced local lesions in genomes)技术作为反向遗传学的一个重要研究工具,其中CEL(celery)Ⅰ酶是TILLING技术的核心内切酶。实验通过硫酸铵沉淀、亲和层析、阴阳离子交换柱层析等常规的蛋白质纯化过程,从芹菜(Apium graveolens)中粗纯化了CELⅠ酶。以杂合双链DNA为底物,检测了CELⅠ酶在不同纯化程度、不同温度、不同处理时间以及有无TaqDNA聚合酶条件下的错配切割活性。结果表明,所获得的CELⅠ酶得到了粗纯化;CELⅠ酶的最适活性温度范围为45~50℃;处理时间长达150min时,CELⅠ酶仍能特异识别并切割错配碱基;在含有TaqDNA聚合酶的酶切体系中,CELⅠ酶错配切割效果更佳。与其它单位提供的CELⅠ酶活性相比,实验粗分离纯化的CELⅠ酶的酶切条带更亮,说明其活性更好。通过对CELⅠ酶的提取及活性分析,为TILLING技术大规模和低成本地应用于家蚕功能基因组研究提供了保障。 | 林英 陈冬妹 赵萍 杨瑜 林立鹏 夏庆友 | 2007 | 农业生物技术学报2007，15，6： | 10 |
| 17 | Identification of in vivo induced protein antigens of Salmonella enterica serovar Typhi during human infection显示文摘During infectious disease episodes, pathogens express distinct subsets of virulence factors which allow them to adapt to different environments. Hence, genes that are expressed or upregulated in vivo are implicated in pathogenesis. We used in vivo induced antigen technology (IVIAT) to identify antigens which are expressed during infection with Salmonella enterica serovar Typhi. We identified 7 in vivo induced (IVI) antigens, which included BcfD (a fimbrial structural subunit), GrxC (a glutaredoxin 3), SapB (an ABC-type transport system), T3663 (an ABC-type uncharacterized transport system), T3816 (a putative rhodanese-related sulfurtransferase), T1497 (a probable TonB-dependent receptor) and T3689 (unknown function). Of the 7 identified antigens, 5 antigens had no cross-immunoreactivity in adsorbed control sera from healthy subjects. These 5 included BcfD, GrxC, SapB, T3663 and T3689. Antigens identified in this study are potential targets for drug and vaccine development and may be utilized as diagnostic agents. | HU Yong1,2, CONG YanGuang1, LI Shu1, RAO XianCai1, WANG Gang3 & HU FuQuan1 1 Department of Microbiology, Third Military Medical University, Chongqing 400038, China 2 Department of Biotechnology, Chongqing University of Technology, Chongqing 400050, China 3 Department of Clinical Laboratory, The 3rd Hospital of People’s Liberation Army, Baoji 721004, China | 2009 | Science China(Life Sciences)2009，52，10： | 9 |
| 18 | Industrial development of gas induced semi-solid process显示文摘The gas induced semi-solid(GISS) is a rheocasting process that produces semi-solid slurry by applying fine gas bubble injection through a graphite diffuser.The process is developed to be used in the die casting industry.To apply the GISS process with a die casting process,a GISS maker unit is designed and attached to a conventional die casting machine with little modifications.The commercial parts are developed and produced by the GISS die casting process.The GISS die casting shows the feasibility to produce industrial parts with aluminum 7075 and A356 with lower porosity than liquid die casting. | S.THANABUMRUNGKUL S.JANUDOM R.BURAPA P.DULYAPRAPHANT J.WANNASIN | 2010 | 中国有色金属学会会刊:英文版2010，20，S3： | 9 |
| 19 | Heavy rockbursts due to longwall mining near protective pillars:A case study显示文摘Rockburst represents a very dangerous phenomenon in deep underground mining in unfavourable conditions such as great depth, high horizontal stress, proximity of important tectonic structures, and unmined pillars. The case study describes a recorded heavy rockburst in the Czech part of the Upper Silesian Coal Basin, which occurred during longwall mining near the protective pillar. The artificial dividing of geological blocks and creation of mining protective pillars(shaft pillars, crosscut pillars etc.) is a dangerous task in light of rockbursts occurring mainly due to overstressing of remaining pillars. A simple model of this situation is presented. Natural and mining conditions are analysed and presented in detail as well as registered seismicity during longwall mining in the area. Recorded rockbursts in the area of interest are described and their causes discussed. Many rockbursts near protective pillars were recorded in this mining region. Methodical instructions for rockburst prevention in proximity of protective pillars as well as for gates driving were devised based on the evaluation of rockburst causes. The paper presents these principles for prevention. | Petr Konicek Jan Schreiber | 2018 | International Journal of Mining Science and Technology2018，28，5： | 9 |
| 20 | Herbal hepatotoxicity:Challenges and pitfalls of causality assessment methods显示文摘The diagnosis of herbal hepatotoxicity or herb induced liver injury(HILI) represents a particular clinical and regulatory challenge with major pitfalls for the causality evaluation.At the day HILI is suspected in a patient,physicians should start assessing the quality of the used herbal product,optimizing the clinical data for completeness,and applying the Council for International Organizations of Medical Sciences(CIOMS) scale for initial causality assessment.This scale is structured,quantitative,liver specific,and validated for hepatotoxicity cases.Its items provide individual scores,which together yield causality levels of highly probable,probable,possible,unlikely,and excluded.After completion by additional information including raw data,this scale with all items should be reported to regulatory agencies and manufacturers for further evaluation.The CIOMS scale is preferred as tool for assessing causality in hepatotoxicity cases,compared to numerous other causality assessment methods,which are inferior on various grounds.Among these disputed methods are the Maria and Victorino scale,an insufficiently qualified,shortened version of the CIOMS scale,as well as various liver unspecific methods such as thead hoc causality approach,the Naranjo scale,the World Health Organization(WHO) method,and the Karch and Lasagna method.An expert panel is required for the Drug Induced Liver Injury Network method,the WHO method,and other approaches based on expert opinion,which provide retrospective analyses with a long delay and thereby prevent a timely assessment of the illness in question by the physician.In conclusion,HILI causality assessment is challenging and is best achieved by the liver specific CIOMS scale,avoiding pitfalls commonly observed with other approaches. | Rolf Teschke Christian Frenzel Johannes Schulze Axel Eickhoff | 2013 | World Journal of Gastroenterology2013，19，19： | 9 |
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