|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Altered Expression of Connexin-43 and Impaired Capacity of Gap Junctional Intercellular Communication in Prostate Cancer Cells显示文摘Connexin-43 (Cx43) expression in prostate cancer (PCa) cells and the potency of gap junctional intercellular communication (GJIC) in the cells were investigated, with an attempt to elu- cidate the reason why the so-called 'bystander effect' mediated by thymidine kinase (TK) suicide gene therapy on PCa cells is not of significance and to explore the role of GJIC in PCa carcinogenesis. mRNA and protein expression of Cx43 in a PCa cell line PC-3m was detected by re- verse-transcription polymerase chain reaction (RT-PCR) and strapt-avidin-biotin-enzyme complex (SABC) immunohistochemical staining, and inherent GJIC of PC-3m cells was assayed by scrape-loading and dye transfer (SLDT) assay. The expression of Cx43 in human normal and malig- nant prostate tissues was determined by SABC immunohistochemistry as well. It was found that Cx43 mRNA and protein expression in PC-3m cells was slightly reduced as compared with positive controls and the location of Cx43 protein was aberrant in cytoplasm rather than on membrane. As- sessment of paraffin sections demonstrated that the expression of Cx43 protein in PCa cells was ab- normally located and markedly diminished as compared with normal prostatic epithelial ones, dis- playing a negative correlation to the pathological grade (χ2=4.025, P<0.05). Additionally, capacity of inherent GJIC in PC-3m cells was disrupted, which was semi-quantified as (+) or (-). It was indi- cated that both down-regulated expression of Cx43 mRNA and aberrant location of Cx43 protein par- ticipated in the mechanisms leading to deficient GJIC in PC-3m cells. Lack of efficient GJIC is a molecular event, which may contribute not only to limited extent of 'bystander effect', but also to initiation and progression of prostatic neoplasm. | 邢毅飞 肖亚军 曾甫清 赵军 肖传国 熊平 冯玮 | 2007 | Journal of Huazhong University of Science and Technology(Medical Sciences)2007，27，3： | 6 |
| 2 | Differences between the gap junctional communication function of fibroblasts derived from pathological scars and normal skin显示文摘Objective: To define the mechanisms underlying the keloid formation. Methods: The gap junctional intercellu- lar commumication (GJIC) of fibroblasts derived from pathological scars and noed skin was investigated. Six Samples of hyperplastic scars, keloids and normal skin were obtained. Fibroblasts from these samples were cultured in ho and vended by type Ⅰ collagen, and the GJIC among the fibroblasts was investigated by adherent cell analysis with soning interactive laser coytometer(ACAS 570) for fibroblasts culturing. Results: The fibroblasts from normal skin showed nounal GJIC, which are depressed between fibroblasts from hyperplastic scare and was completely blocked in fibroblasts from the keloids. Conclusion: The blockade of interoellular communication may play an important role in the pathogenesis of excessive and invasive growth of fibroblasts derived from the keloids. | 鲁峰 高建华 黎小间 | 2000 | Journal of Medical Colleges of PLA(China)2000，15，2： | 4 |
| 3 | Microfluidic fabrication of water-in-water droplets encapsulated in hydrogel microfibers显示文摘Aqueous microdroplets are of significant interest in biological fields, while the employment of water-inoil (w/o) emulsion and lack of spatial control precluded its widespread application. Herein a novel microfluidic approach is developed to generate water-in-water (w/w) microdroplets embedded in hydrogel microfibers. Aqueous two phase system (ATPS) is applied to generate w/w droplets, and alginate is introduced to continuous phase to form microfibers, which offers spatial restriction and manipulation possibility to droplets. The size and pattern of aqueous droplets can be precisely controlled, and immobilization within hydrogel fiber facilitates easy manipulation and observation. The microdroplets surrounded by hydrophilic environment can act as a cell-cell interaction model, and their potential for biological and environmental applications are demonstrated by long-term culture of encapsulated cells and water remediation of Bacillus subtilis. | Chenguang Liu Wenchen Zheng Ruoxiao Xie Yupeng Liu Zhe Liang Guoan Luo Mingyu Ding Qionglin Liang | 2019 | Chinese Chemical Letters2019，30，2： | 2 |
| 4 | Mechanism of changes in gap junctional intercellular communication in myocardial cells after burns显示文摘Objective: To explore the pathophysiological mechanism of the changes in gap junctionalintercellular communication (GJIC) in the myocardial cells after burns. Methods: After the myocardial cellswere cultured and injured with hypoxia and burn serum, the GJIC in the cells was detected with scrapeloading and dye transfer. Meanwhile, the viability, cytosolic free Ca2+ concentration and Ca2+ influx of themyocardial cells were determined. Results: The cytosolic free Ca2+ concentration and the cellulartransmembrane Ca2+ influx were significantly increased but the viability of the cells markedly decreased afterthe injury. The LY fluorescence reached 4 rows of cells from the scrape line in the normal myocardial cells.The GJIC was blocked at the first hour after hypoxia or hypoxia and burn serum injury. The LY fluorescencewas limited to the primary loads cells at the sixth hour after hypoxia and the third hour after hypoxia andburn serum injury. Conclusion: The function of GJIC in the myocardial cells is to maintain high ordersynchronous contraction of the myocardium. After burns, the runaway calcium homeostasis and impairmentof GJIC function would be accused to be the pathological basis for myocardial heterogeneous behavior. | 迟路湘 杨宗诚 王旭 黎鳌 | 1999 | Journal of Medical Colleges of PLA(China)1999，14，1： | 0 |
| 5 | Research progress on radiation-induced bystander effect显示文摘Radiation-induced bystander effect is the phenomenon that the cells which are not directly exposed to radiation have identical or similar biological reactions with the cells of direct exposure to radiation. It is a common second reaction of radiotherapy and has a strong impact on cancer patients. Here we review and synthesize its studies in vitro and in vivo, its time effect and the mechanism. And the existing problems and its research significance are also discussed. | Chao-Ning Zhang Jin-Tian Li | 2019 | TMR Cancer2019，2，2： | 0 |
| 6 | EFFECTS OF LIMONENE,SALVIA MILTIORRHIZA AND TURMERIC DERIVATIVES ON H-RAS ONCOGENE EXPRESSION AND GAP JUNCTION INTERCELLULAR COMMUNICATION IN HUMAN SOLID TUMOR CELL LINES显示文摘Oncogenicrasproteinsarecausalyimplicatedcertainhumanmalignancieswithabout30-40%ofhumanlungadenocarcinomas,50%humancoloncarcin... | 陈晓光 连间忠芳 矢野善久 吉都俣士子 大谷周造 | 1998 | Chinese Journal of Cancer Research1998，10，3： | 0 |
| 7 | Basic Investigations EXPRESSION OF GAP JUNCTION PROTEIN Cx43 IN CULTURED HUMAN NORMAL AND MALIGNANT LUNG CELLS显示文摘BasicInvestigationsEXPRESSIONOFGAPJUNCTIONPROTEINCx43INCULTUREDHUMANNORMALANDMALIGNANTLUNGCELLSZhangZhiqian;张志谦;LinZhongxiang... | 张志谦 林仲翔 吕有勇 孟松娘 韩亚玲 | 1994 | Chinese Journal of Cancer Research1994，6，2： | 0 |
| 8 | Functional expression of gap junction gene Cx43 and the myogenic differentiation of rhabdomyosarcoma cells显示文摘Rhabdomyosarcoma (RD) cells express low levels of the gap junction protein connexin 43 (Cx43), and its mRNA, and display very weak gap junctional intercellular communication (GJIC) as detected by Cx43 immunofluorescence, slot-blot and dye-transfer methods. These cells grow rapidly and show aberrant and incomplete myogenic differentiation. To investigate the role of gap junctions in these cells, the expression of Cx43 with relation to cell growth and myogenic differentiation in RD single-cell subclones-RDL3 and RDL6 is studied. The subclone RDL3 grows slowly and displays better myogenic differentiation. The expression of Cx43, its mRNA and the GJIC in RDL3 is comparable to that in normal myoblasts. Another subclone RDL6 which grows rapidly, but is poorly differentiated, expresses very low levels of Cx43 and its mRNA, and very weak GJIC. By using the calcium phosphate precipitate transfection technique, a full-length cDNA-encoding Cx43 and a pSV2neo have been introduced into the RDL6 cells. Several | 林仲翔 张志谦 韩亚玲 C.C.G.Naus K.R.Yu H.Holtzer | 1995 | Science China Chemistry1995，38，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员