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| 1 | Non invasive blood flow measurement in cerebellum detects minimal hepatic encephalopathy earlier than psychometric tests显示文摘AIM: To assess whether non invasive blood flow measurement by arterial spin labeling in several brain regions detects minimal hepatic encephalopathy. METHODS: Blood flow(BF) was analyzed by arterial spin labeling(ASL) in different brain areas of 14 controls, 24 cirrhotic patients without and 16 cirrhotic patients with minimal hepatic encephalopathy(MHE). Images were collected using a 3 Tesla MR scanner(Achieva 3T-TX, Philips, Netherlands). Pulsed ASL was performed. Patients showing MHE were detected using the battery Psychometric Hepatic Encephalopathy Score(PHES) consisting of five tests. Different cognitive and motor functions were also assessed: alterations in selective attention were evaluated using the Stroop test. Patients and controls also performed visuo-motor and bimanual coordination tests. Several biochemical parameters were measured: serum pro-inflammatory interleukins(IL-6 and IL-18), 3-nitrotyrosine, cGMP and nitrates+nitrites in plasma, and blood ammonia. Bivariate correlations were evaluated.RESULTS: In patients with MHE, BF was increased in cerebellar hemisphere(P = 0.03) and vermis(P = 0.012) and reduced in occipital lobe(P = 0.017). BF in cerebellar hemisphere was also increased in patients without MHE(P = 0.02). Bimanual coordination was impaired in patients without MHE(P = 0.05) and much more in patients with MHE(P < 0.0001). Visuo-motor coordination was impaired only in patients with MHE(P < 0.0001). Attention was slightly affected in patients without MHE and more strongly in patients with MHE(P< 0.0001). BF in cerebellar hemisphere and vermis correlated with performance in most tests of PHES [(number connection tests A(NCT-A), B(NCT-B)and line tracing test] and in the congruent task of Stroop test. BF in frontal lobe correlated with NCT-A. Performance in bimanual and visuomotor coordination tests correlated only with BF in cerebellar hemisphere. BF in occipital lobe correlates with performance in the PHES battery and with CFF. BF in cerebellar hemisphere correlates with plasma cGMP and nitric oxide(NO) metabolites. BF in vermis cerebellar also correlates with NO metabolites and with 3-nitrotyrosine. IL-18 in plasma correlates with BF in thalamus and occipital lobe.CONCLUSION: Non invasive BF determination in cerebellum using ASL may detect MHE earlier than the PHES. Altered NO-cGMP pathway seems to be associated to altered BF in cerebellum. | Vicente Felipo Amparo Urios Carla Giménez-Garzó Omar Cauli Maria-Jesús Andrés-Costa Olga González Miguel A Serra Javier Sánchez-González Roberto Aliaga Remedios Giner-Durán Vicente Belloch Carmina Montoliu | 2014 | World Journal of Gastroenterology2014，20，33： | 12 |
| 2 | Stable isotope labelled mass spectrometry for quantification of the relative abundances for expressed proteins induced by PeaT1显示文摘The protein elicitor from the mycelium of Alternaria tenuissima has been isolated.The elicitor triggered resistance to the tobacco mosaic virus in tobacco by inducing relative oxygen species,but without causing hypersensitive necrosis.The elicitor is reported to impart resistance against Verticillium dahliae and to increase yield in cotton,but its mechanism is not yet clear.In this study,the stable isotope labelled mass spectrometry method was used to quantify the relative abundances of protein expression induced by PeaT1 in Arabidopsis.A significant difference in the relative abundances for the expression of different proteins related to metabolism,modification,regulatory,defense,stress and antioxidation was found in Arabidopsis. | LI GuangYue,YANG XiuFen,ZENG HongMei,MAO JianJun,LIU Hua,ZHANG YunHua & QIU DeWen* Key Laboratory for Biological Control of the Ministry of Agriculture,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100081,China | 2010 | Science China(Life Sciences)2010，53，12： | 7 |
| 3 | Paddy System with a Hybrid Rice Enhances Cyanobacteria Nostoc and Increases N2 Fixation显示文摘Biological nitrogen (N) fixation (BNF) plays a significant role in maintaining soil fertility in paddy field ecosystems. Rice variety influences BNF, but how different rice varieties regulate BNF and associated diazotroph communities has not been quantified. Airtight, field-based 15N2-labelling grow th chamber experiments were used to assess the BNF capac 辻 y of different rice varie ties. In addition, both the 16S rRNA and nifH genes were sequenced to assess the influence of different rice varieties on bacterial and diazotrophic communities in paddy soils. After subjecting a rice-soil system to 74 d of continuous airtight, field-based 15N2 labelling in pots in a growth chamber, the amounts of fixed N were 22.3 and 38.9 kg ha^-1 in inbred japonica (W23) and hybrid indica (IIY) rice cultivars plan ted in the rice-soil systems, respectively, and only 1%—2.5% of the fixed N was allocated to the rice plants and weeds. A greater abundance of diazotrophs was found in the surface soil (0-1 cm) under IIY than under W23. Sequencing of the 16S rRNA gene showed significantly greater abundances of the cyanobacterial genera Nostoc, Anabaena, and Cylindrospermum under IIY than under W23. Sequencing of the nifH gene also showed a significantly greater abundance of Nostoc under IIY than under W23. These results indicate that the hybrid rice cultivar (IIY) promoted BNF to a greater extent than the inbred rice cultivar (W23) and that the increase in BNF might have been due to the enhanced heterocystous cyanobacteria Nostoc. | MA Jing BEI Qicheng WANG Xiaojie LIU Gang Georg CADISCH LIN Xingwu ZHU Jianguo SUN Xiaoli XIE Zubin | 2019 | Pedosphere2019，29，3： | 7 |
| 4 | Interactive labelling of a multivariate dataset for supervised machine learning using linked visualisations,clustering,and active learning显示文摘Supervised machine learning techniques require labelled multivariate training datasets.Many approaches address the issue of unlabelled datasets by tightly coupling machine learning algorithms with interactive visualisations.Using appropriate techniques,analysts can play an active role in a highly interactive and iterative machine learning process to label the dataset and create meaningful partitions.While this principle has been implemented either for unsupervised,semi-supervised,or supervised machine learning tasks,the combination of all three methodologies remains challenging.In this paper,a visual analytics approach is presented,combining a variety of machine learning capabilities with four linked visualisation views,all integrated within the mVis(multivariate Visualiser)system.The available palette of techniques allows an analyst to perform exploratory data analysis on a multivariate dataset and divide it into meaningful labelled partitions,from which a classifier can be built.In the workflow,the analyst can label interesting patterns or outliers in a semi-supervised process supported by active learning.Once a dataset has been interactively labelled,the analyst can continue the workflow with supervised machine learning to assess to what degree the subsequent classifier has effectively learned the concepts expressed in the labelled training dataset.Using a novel technique called automatic dimension selection,interactions the analyst had with dimensions of the multivariate dataset are used to steer the machine learning algorithms.A real-world football dataset is used to show the utility of mVis for a series of analysis and labelling tasks,from initial labelling through iterations of data exploration,clustering,classification,and active learning to refine the named partitions,to finally producing a high-quality labelled training dataset suitable for training a classifier.The tool empowers the analyst with interactive visualisations including scatterplots,parallel coordinates,similarity maps for records,and a new similarity map for partitions. | Mohammad Chegini Jürgen Bernard Philip Berger Alexei Sourin Keith Andrews Tobias Schreck | 2019 | Visual Informatics2019，3，1： | 3 |
| 5 | Therapeutic potential of menstrual blood stem cells in treating acute liver failure显示文摘BACKGROUND Acute liver failure(ALF)is a significant and complex hepatic insult that may rapidly progress to life-threatening conditions.Recently,menstrual blood stem cells(MenSCs)have been identified as a group of easily accessible mesenchymal stem cells with the advantages of non-invasive acquisition,low immunogenicity,a greater capacity of self-renewal and multi-lineage differentiation,making them promising candidates for stem cell-based therapy to revolutionize the treatment strategies for liver failure.AIM To investigate the therapeutic potential of MenSCs for treating ALF in pigs and to dynamically trace the biodistribution of transplanted cells.METHODS MenSCs were labeled in vitro with PKH26,a lipophilic fluorescent dye.The treatment group received immediate transplantation of PKH26-labelled MenSCs(2.5×106/kg)via the portal vein after D-galactosamine injection,and the control group underwent sham operation.The survival time,liver function,and hepatic pathological changes were compared between the two groups.Three major organs(liver,lungs and spleen)were extracted from animals and imaged directly with the In vivo Imaging System(IVIS)at the predetermined time points.The regions of interest were drawn to quantify the cell uptake in different organs.RESULTS The labelling procedure did not affect the morphology,viability or multipotential differentiation of MenSCs.Biochemical analysis showed that the levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBIL)and prothrombin time(PT)measured at selected time points 24 h after transplantation were significantly decreased in the treatment group(P<0.05).The survival time of ALF animals was prolonged in the treatment group compared with the control group(75.75±5.11 h vs 53.75±2.37 h,log rank,P<0.001).The liver pathological tissue in the MenSC treatment group showed obviously increased numbers of remaining hepatocytes and a comparatively slight necrotic degree and area.In addition,the IVIS imaging revealed that PKH26-positive MenSCs were clearly retained in the liver initially and then diffused through the systemic circulation.Interestingly,the signal intensity in the liver increased obviously at 36 h,which corresponded to the biochemical result that liver function deteriorated most rapidly at 24-36 h.CONCLUSION Our study demonstrates the therapeutic efficacy and homing ability of transplanted MenSCs in a large animal model of ALF and suggests that MenSC transplantation could be a promising strategy for treating ALF. | Pan-Pan Cen Lin-Xiao Fan Jie Wang Jia-Jia Chen Lan-Juan Li | 2019 | World Journal of Gastroenterology2019，25，41： | 2 |
| 6 | Applications of single-cell technology on bacterial analysis显示文摘Background: Traditionally,scientists studied microbiology through the manner of batch cultures,to conclude the dynamics or outputs by averaging all individuals.However,as the researches go further,the heterogeneities among the individuals have been proven to be crucial for the population dynamics and fates.Results:Due to the limit of technology,single-cell analysis methods were not widely used to decipher the inherent connections between individual cells and populations.Since the early decades of this century,the rapid development of microfluidics,fluorescent labelling,next-generation sequencing,and high-resolution microscopy have speeded up the development of single-cell technologies and further facilitated the applications of these technologies on bacterial analysis.Conclusions:In this review,we summarized the recent processes of single-cell technologies applied in bacterial analysis in terms of intracellular characteristics,cell physiology dynamics,and group behaviors,and discussed how single-cell technologies could be more applicable for future bacterial researches. | Zhixin Ma Pan MChu Yingtong Su Yue Yu Hui Wen Xiongfei Fu Shuqiang Huang | 2019 | Quantitative Biology2019，7，3： | 2 |
| 7 | THE CYCLIC BANDWIDTH PROBLEM显示文摘THECYCLICBANDWIDTHPROBLEM￥LINYixun(DepartmentofMathematics,ZhengzhouUniversity,Zhezhou450052,China)Abstract:Thecyclicbandwidt... | LIN Yixun (Department of Mathematics, Zhengzhou University, Zhezhou 450052, China) | 1994 | Systems Science and Mathematical Sciences1994，7，3： | 1 |
| 8 | EFFECT OF SUPERNATANT OF PERIPHERAL BLOOD MONONULEAR CELLS FROM NEPHROTIC PATIENTS ON THE SULFATED COMPOUND SYNTHESIS BY CULTURED GLOMERULAR EPITHELIAL CELL显示文摘To identify the role of circulating immune factor(s) in the development of proteinuria, the supernatant of peripheral blood mononuclear cells (PBMC) were obtained from 15 patients with steriod-responsive nephrotie syndrome, and its effect on the synthesis of sulfated compound by cultured rat glomerular epithelial cell (GEC) were studied by 35S, 3H-leucine dual-isotope labelling technique. The result showed that the supernatant of PBMC from 9 patients without steroid treat ment increased 35S uptake in GEC (P<0.01), while there was no significant difference in 35S uptake between the 6 patients treated with prednisone (1.5-2.0 mg/kg.d-1) and 6 normal controls. | 王丹 杨霁云 王宝琳 | 1994 | Chinese Medical Journal1994，，6： | 1 |
| 9 | STUDIES ON THE RADIOIODINATION BY IODOGEN显示文摘In 1978, P. Fraker and J. Speck first used the iodogen (or 1, 3, 4, 6-tetrachloro3a, 6a-diphenyglycoluril) in the radioiodination of bioactive materials such as γ-globulins, lysozyme and cell membranes.This new technique has been applied gradually to biochemistry, medical researches and other fields. Iodogen is an ideal electron | 薛嘉渔 李永键 | 1986 | Chinese Science Bulletin1986，31，14： | 0 |
| 10 | Morphological properties of nociceptive and non-nociceptive neurons in primary somatic cerebral cortex (SI) of cat显示文摘With the techniques of intracellular recording and labelling, we investigated pain sensation and modulation of the somatic cortical cortex at the neuron’s level. After observing the evoked potentials from stimulating the saphenous nerves (SN) of 654 neurons in SI area of the cats, we labelled 30 of the neurons with Neurobiotin to preserve the distribution and the morphologic characteristics of the neurons in the cortex. Based on the tridimensional reconstruction in addition to the eletrophysiological functions, we found clear morphological distinctions between nociceptive and non-nociceptive neurons (P<0.01). This result provided new experimental material to illustrate the function of nociceptive neurons in somatosensory cortex (SI) and presented further evidence to support the 'specificity theory' of pain sensation in terms of morphology. | Rihui Zhang Guoxi Teng | 2000 | Chinese Science Bulletin2000，45，22： | 0 |
| 11 | 双圈连通图的L(2,1)-labelling(英文)显示文摘给定图G,G的一个L(2,1)-labelling是指一个映射f:V(G)→{0,1,2,…},满足:当dG(u,v)=1时,|f(u)-f(v)|≥2;当dG(u,v)=2时,|f(u)-f(v)|≥1。如果G的一个L(2,1)-labelling的像集合中没有元素超过k,则称之为一个k-L(2,1)- labelling.G的L(2,1)-labelling数记作l(G),是指使得G存在k-L(2,1)-labelling的最小整数k.如果G的一个L(2,1)-labelling中的像元素是连续的,则称之为一个no-hole L(2,1)-labelling.本文证明了对每个双圈连通图G,l(G)=△+1或△+2.这个工作推广了[1]中的一个结果.此外,我们还给出了双圈连通图的no-hole L(2,1)-labelling的存在性. | 翟明清 吕长虹 | 2008 | 运筹学学报2008，12，1： | 0 |
| 12 | CATALYTIC LABELLING AND ~3H NMR ANALYSES OF SOME AROMATIC AND HETEROCYCLIC COMPOUNDS显示文摘This paper described the ~3H NMR determination of the positions and content of 24 tritium labeled aromatic and heterocyclic compounds prepared by catalytic tritiation derivatives, pyrrole, 1,2,4-triazole, thiazole, benzothiazole, purine and derivatives, indole, indanol, piperidine and derivative, diphenylamine, N- phenylanthranilic acid, 8- OH- quinoline, naphthol, diphenylether have been tritiated by catalytic exchange method using tritiated water and platinum catalyst (from the dioxide and sodium borohydride), and the pattern of labelling has been assigned by ~3H NMR spectroscopy. The results show that this exchange process can give general labelling compounds at various time and temperatures. For 4-Br-diphenylether, Raney nickel was used instead of Pt as a catalyst. | 张丽明 John R. Jones | 1991 | Nuclear Science and Techniques1991，2，3： | 0 |
| 13 | Interactive visual labelling versus active learning:an experimental comparison显示文摘Methods from supervised machine learning allow the classification of new data automatically and are tremendously helpful for data analysis.The quality of supervised maching learning depends not only on the type of algorithm used,but also on the quality of the labelled dataset used to train the classifier.Labelling instances in a training dataset is often done manually relying on selections and annotations by expert analysts,and is often a tedious and time-consuming process.Active learning algorithms can automatically determine a subset of data instances for which labels would provide useful input to the learning process.Interactive visual labelling techniques are a promising alternative,providing effective visual overviews from which an analyst can simultaneously explore data records and select items to a label.By putting the analyst in the loop,higher accuracy can be achieved in the resulting classifier.While initial results of interactive visual labelling techniques are promising in the sense that user labelling can improve supervised learning,many aspects of these techniques are still largely unexplored.This paper presents a study conducted using the mVis tool to compare three interactive visualisations,similarity map,scatterplot matrix(SPLOM),and parallel coordinates,with each other and with active learning for the purpose of labelling a multivariate dataset.The results show that all three interactive visual labelling techniques surpass active learning algorithms in terms of classifier accuracy,and that users subjectively prefer the similarity map over SPLOM and parallel coordinates for labelling.Users also employ different labelling strategies depending on the visualisation used. | Mohammad CHEGINI Jurgen BERNARD Jian CUI Fatemeh CHEGINI Alexei SOURIN Keith ANDREWS Tobias SCHRECK | 2020 | Frontiers of Information Technology & Electronic Engineering2020，21，4： | 0 |
| 14 | 近期外文文献题录(英文)显示文摘 | 喻萍 | 2006 | 冷饮与速冻食品工业2006，12，4： | 0 |
| 15 | ON THE COMPLEXITY OF A PL HOMOTOPY ALGORITHM FOR ZEROES OF POLYNOMIALS显示文摘A PL homotopy method (KNA method in short) was presented in Ref.[1] for the problem of locating zeroes of any polynomial of form f(z)=zn+sum from k=1 to n ak zn-k. Due to the introduction of a perturbation term, the method gives all zeroes of a polynomial with correct multiplicities. However, there are no error estimates independent from the coefficients, and thus no results of complexity. | 高堂安 王则柯 | 1991 | Chinese Science Bulletin1991，36，6： | 0 |
| 16 | Highly efficient labelling of extracellular vesicles for enhanced detection on a microfluidic platform显示文摘Developing precise extracellular vesicles(EVs)labelling techniques with minimal disturbance is of great importance to the follow-up EVs detection and analysis.However,currently available methods such as using probes to conjugate phospholipids or membrane proteins have certain limitations due to EV steric hindrance,dye aggregation,etc.Here,we present a microfluidic platform to enhance EVs’labelling efficiency and improve their detection.This platform provides excellent sample throughput and high-efficiency EV labelling at lower label concentrations with an optimized flowing rate.Flow cytometry analysis(FCM)and cellular uptake results show that EV labelling by utilizing this platform possesses the merits of a higher labelling efficiency with 64.1%relative improvement than conventional co-incubation method and a lower background noise.Moreover,this technique maintains EVs’size,morphology and biological activities.After the recipient cells uptake the EVs treated by the microfluidic platform,the spatial and temporal distribution of EVs in the cells are clearly observed.These results demonstrate that our method holds great potential in efficient labelling of EVs,which is essential to subsequent EV quantification and analysis. | Shi Hu Rui Hao Zitong Yu Huitao Zhang Hui Yang | 2024 | Chinese Chemical Letters2024，35，2： | 0 |
| 17 | ON THE COMPLEXITY OF A PL HOMOTOPY ALGORITHM FOR ZEROS OF POLYNOMIALS显示文摘A PL homotopy algorithm is modified to yield a polynomial-time result on its computational complexity.We prove that the cost of locating all zeros of a polynomial of degree n to an accuracy of ε(measured by the number of evaluations of the polynomial)grows no faster than O(max{n4,n3log2(n/ε)}).This work is in response to a question raised in a paper by S.Smale as to the efficiency of piecewise linear methods in solving equations.In comparison with a few results reported,the algorithm under discussion is the only one providing correct multiplicities and the only one employing vector labelling. | 高堂安 王则柯 | 1993 | Acta Mathematicae Applicatae Sinica1993，9，2： | 0 |
| 18 | IDENTIFICATION OF THE fi FEATURE OF THE DRUG RESISTANT PLASMIDS pFD10 AND pFD11 IN E. COLI显示文摘This paper deals with a method of identifying, with transposon, the fertility inhibition feature of plasmids. Using the method of labelling the F′lac pro plasmid with transposon Tn5, we identified the fi feature of the drug resistant plasmids pFD10 and pFD11 with R144 as a control. The results obtained by this method are identical with that by the recombination method. However, the former is simple and more convenient. When the pFD10 plasmid coexisted with the F′lac pro plasmid, the fre- | 孔祥福 程莉莉 陈孝康 | 1982 | Chinese Science Bulletin1982，27，3： | 0 |
| 19 | Spatial patterns nitrogen transfer models of ectomycorrhizal networks in a Mongolian scotch pine plantation显示文摘Ectomycorrhizal(EM)networks provide a variety of services to plants and ecosystems include nutrient uptake and transfer,seedling survival,internal cycling of nutrients,plant competition,and so on.To deeply their structure and function in ecosystems,we investigated the spatial patterns and nitrogen(N)transfer of EM networks using ^(15)N labelling technique in a Mongolian scotch pine(Pinus sylvestris var.mongolica Litv.)plantation in Northeastern China.In August 2011,four plots(20 × 20 m)were set up in the plantation.125 ml 5 at.%0.15 mol/L ^(15)NH_4 ^(15)NO_3solution was injected into soil at the center of each plot.Before and 2,6,30 and 215 days after the ^(15)N application,needles(current year)of each pine were sampled along four 12 m sampling lines.Needle total N and ^(15)N concentrations were analyzed.We observed needle N and^(15)N concentrations increased significantly over time after ^(15)N application,up to 31 and0.42%,respectively.There was no correlation between needle N concentration and ^(15)N/ ^(14)N ratio(R2=0.40,n=5,P=0.156),while excess needle N concentration and excess needle ^(15)N/^(14)N ratio were positively correlated across different time intervals(R^2=0.89,n=4,P\0.05),but deceased with time interval lengthening.Needle ^(15)N/^(14)N ratio increased with time,but it was not correlated with distance.Needle ^(15)N/ ^(14)N ratio was negative with distance before and 6th day and 30th day,positive with distance at 2nd day,but the trend was considerably weaker,their slop were close to zero.These results demonstrated that EM networks were ubiquitous and uniformly distributed in the Mongolian scotch pine plantation and a random network.We found N transfer efficiency was very high,absorbed N by EM network was transferred as wide as possible,we observed N uptake of plant had strong bias for ^(14)N and ^(15)N,namely N fractionation.Understanding the structure and function of EM networks in ecosystems may lead to a deeper understanding of ecological stability and evolution,and thus provide new theoretical approaches to improve conservation practices for the management of the Earth’s ecosystems. | Yanbin Liu Hongmei Chen Pu Mou | 2018 | Journal of Forestry Research2018，29，2： | 0 |
| 20 | THE EFFECT OF DELTAMETHRIN ON PROTEIN PHOSPHORYLATION OF MOUSE BRAIN in vitro显示文摘The mode of action of deltamethrin as a neurotoxic insecticide being widespreadly used has not been clearly established to date. The present note deals with the effect of deltamethrin on endogenous protein phosphorylation of mouse brain in vitro, which may be helpful to understanding the mechanism of action of deltame- | 姜建成 冷欣夫 | 1988 | Chinese Science Bulletin1988，33，18： | 0 |
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