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Stable isotope labelled mass spectrometry for quantification of the relative abundances for expressed proteins induced by PeaT1

查看全文 作  者:LI GuangYue,YANG XiuFen,ZENG HongMei,MAO JianJun,LIU Hua,ZHANG YunHua & QIU DeWen* Key Laboratory for Biological Control of the Ministry of Agriculture,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100081,China 高影响力作者 出  处:《Science China(Life Sciences)》索引2010年第53卷第12期,共8页高影响力期刊 基  金:supported by the Hi-Tech Research and Development Program of China (Grant No. 2006AA10A210) 摘  要:The protein elicitor from the mycelium of Alternaria tenuissima has been isolated.The elicitor triggered resistance to the tobacco mosaic virus in tobacco by inducing relative oxygen species,but without causing hypersensitive necrosis.The elicitor is reported to impart resistance against Verticillium dahliae and to increase yield in cotton,but its mechanism is not yet clear.In this study,the stable isotope labelled mass spectrometry method was used to quantify the relative abundances of protein expression induced by PeaT1 in Arabidopsis.A significant difference in the relative abundances for the expression of different proteins related to metabolism,modification,regulatory,defense,stress and antioxidation was found in Arabidopsis. 关 键 词:protein ELICITOR stable ISOTOPE labelling relative ABUNDANCES PeaT1
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