|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Cell–cell contact with proinflammatory macrophages enhances the immunotherapeutic effect of mesenchymal stem cells in two abortion models显示文摘Mesenchymal stem cells(MSCs),which are pluripotent cells with immunomodulatory properties,have been considered good candidates for the therapy of several immune disorders,such as inflammatory bowel diseases,concanavalin A-induced liver injury,and graft-versus-host disease.The embryo is a natural allograft to the maternal immune system.A successful pregnancy depends on the timely extinction of the inflammatory response induced by embryo implantation,followed by the switch to a tolerant immune microenvironment in both the uterus and the system.Excessive infiltration of immune cells and serious inflammatory responses are triggers for embryo rejection,which results in miscarriage.Here,we demonstrated that adoptive transfer of MSCs could prevent fetal loss in a lipopolysaccharide(LPS)-induced abortion model and immune response-mediated spontaneous abortion model.The immunosuppressive MSCs alleviated excessive inflammation by inhibiting CD4+T cell proliferation and promoting the decidual macrophage switch to M2 in a tumor necrosis factor-stimulated gene-6(TSG-6)-dependent manner.Cell-tocell contact with proinflammatory macrophages increased the TSG-6 production by the MSCs,thereby enhancing the suppressive regulation of T cells and macrophages.Moreover,proinflammatory macrophages in contact with the MSCs upregulated the expression of CD200 on the stem cells and facilitated the reprogramming of macrophages towards an anti-inflammatory skew through the interaction of CD200 with CD200R on proinflammatory macrophages.Therefore,the results demonstrate that a TSG-6-mediated paracrine effect,reinforced by cell-to-cell contact between MSCs and proinflammatory macrophages,is involved in the mechanism of MSC-mediated abortion relief through the induction of immune tolerance.Our study also indicates the potential application of MSCs in clinical recurrent miscarriages. | Yanhong Li Di Zhang Ling Xu Lin Dong Ji Zheng Yikong Lin Jiefang Huang Yanyun Zhang Yu Tao Xingxing Zang Dajin Li Meirong Du | 2019 | Cellular & Molecular Immunology2019，16，12： | 23 |
| 2 | Genome sequences of horticultural plants:past,present,and future显示文摘Horticultural plants play various and critical roles for humans by providing fruits,vegetables,materials for beverages,and herbal medicines and by acting as ornamentals.They have also shaped human art,culture,and environments and thereby have influenced the lifestyles of humans.With the advent of sequencing technologies,there has been a dramatic increase in the number of sequenced genomes of horticultural plant species in the past decade.The genomes of horticultural plants are highly diverse and complex,often with a high degree of heterozygosity and a high ploidy due to their long and complex history of evolution and domestication.Here we summarize the advances in the genome sequencing of horticultural plants,the reconstruction of pan-genomes,and the development of horticultural genome databases.We also discuss past,present,and future studies related to genome sequencing,data storage,data quality,data sharing,and data visualization to provide practical guidance for genomic studies of horticultural plants.Finally,we propose a horticultural plant genome project as well as the roadmap and technical details toward three goals of the project. | Fei Chen Yunfeng Song Xiaojiang Li Junhao Chen Lan Mo Xingtan Zhang Zhenguo Lin Liangsheng Zhang | 2019 | Horticulture Research2019，6，1： | 16 |
| 3 | Role of microparticles in endothelial dysfunction and arterial hypertension显示文摘Microparticles are small cell vesicles that can be released by almost all eukaryotic cells during cellular stress and cell activation. Within the last 1-2 decades it has been shown that microparticles are useful blood surrogate markers for different pathological conditions, such as vascular inflammation, coagulation and tumour diseases. Several studies have investigated the abundance of microparticles of different cellular origins in multiple cardiovascular diseases. It thereby has been shown that microparticles released by platelets, leukocytes and endothelial cells can be found in conditions of endothelial dysfunction, acute and chronic vascular inflammation and hypercoagulation. In addition to their function as surrogate markers, several studies indicate that circulating microparticles can fuse with distinct target cells, such as endothelial cells or leukocyte, and thereby deliver cellular components of their parental cells to the target cells. Hence, microparticles are a novel entity of circulating, paracrine, biological vectors which can influence the phenotype, the function and presumably even the transcriptome of their target cells.This review article aims to give a brief overview about the microparticle biology with a focus on endothelial activation and arterial hypertension. More detailed information about the role of microparticles in pathophysiology and disease can be found in already published work. | Thomas Helbing Christoph Olivier Christoph Bode Martin Moser Philipp Diehl | 2014 | World Journal of Cardiology2014，6，11： | 14 |
| 4 | RAB31 marks and controls an ESCRT-independent exosome pathway显示文摘Exosomes are generated within the multivesicular endosomes(MVEs)as intraluminal vesicles(ILVs)and secreted during the fusion of MVEs with the cell membrane.The mechanisms of exosome biogenesis remain poorly explored.Here we identify that RAB31 marks and controls an ESCRT-independent exosome pathway.Active RAB31,phosphorylated by epidermal growth factor receptor(EGFR),engages flotillin proteins in lipid raft microdomains to drive EGFR entry into MVEs to form ILVs,which is independent of the ESCRT(endosomal sorting complex required for transport)machinery.Active RAB31 interacts with the SPFH domain and drives ILV formation via the Flotillin domain of flotillin proteins.Meanwhile,RAB31 recruits GTPase-activating protein TBC1D2B to inactivate RAB7,thereby preventing the fusion of MVEs with lysosomes and enabling the secretion of ILVs as exosomes.These findings establish that RAB31 has dual functions in the biogenesis of exosomes:driving ILVs formation and suppressing MVEs degradation,providing an exquisite framework to better understand exosome biogenesis. | Denghui Wei Weixiang Zhan Ying Gao Liyan Huang Run Gong Wen Wang Ruhua Zhang Yuanzhong Wu Song Gao Tiebang Kang | 2021 | Cell Research2021，31，2： | 12 |
| 5 | The ORF3a protein of SARS-CoV-2 induces apoptosis in cells显示文摘Severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)has caused the ongoing pandemic of Coronavirus Disease 2019.SARS-CoV-2 belongs to the genus Betacoronavirus of the Coronaviridae family,which includes SARS-CoV and Middle East respiratory syndrome coronavirus.1,2 Coronavirus-encoded accessory proteins play critical roles in virus–host interactions and the modulation of host immune responses,thereby contributing to coronaviral pathogenicity via different strategies.3 However,the functions of SARS-CoV-2-encoded accessory proteins are not well understood.Apoptosis is a predominant type of programmed cell death,and has been recognized as an important host antiviral defense mechanism that controls viral infection and regulates the inflammatory response.4,5 Previous studies have reported that the SARS-CoV-encoded accessory protein ORF3a can induce apoptosis in cells,6,7 leading to the question of whether SARS-CoV-2 ORF3a also has pro-apoptotic activity.Here,we investigated the potential apoptosis-inducing activity of SARS-CoV-2 ORF3a in different cell lines and compared the pro-apoptotic activities of SARS-CoV-2 ORF3a with those of SARS-CoV ORF3a using the same system. | Yujie Ren Ting Shu Di Wu Jingfang Mu Chong Wang Muhan Huang Yang Han Xue-Yi Zhang Wei Zhou Yang Qiu Xi Zhou | 2020 | Cellular & Molecular Immunology2020，17，8： | 11 |
| 6 | Convolutional Neural Networks for Image-Based High-Throughput Plant Phenotyping:A Review显示文摘Plant phenotyping has been recognized as a bottleneck for improving the efficiency of breeding programs,understanding plantenvironment interactions,and managing agricultural systems.In the past five years,imaging approaches have shown great potential for high-throughput plant phenotyping,resulting in more attention paid to imaging-based plant phenotyping.With this increased amount of image data,it has become urgent to develop robust analytical tools that can extract phenotypic traits accurately and rapidly.The goal of this review is to provide a comprehensive overview of the latest studies using deep convolutional neural networks(CNNs)in plant phenotyping applications.We specifically review the use of various CNN architecture for plant stress evaluation,plant development,and postharvest quality assessment.We systematically organize the studies based on technical developments resulting from imaging classification,object detection,and image segmentation,thereby identifying state-of-the-art solutions for certain phenotyping applications.Finally,we provide several directions for future research in the use of CNN architecture for plant phenotyping purposes. | Yu Jiang Changying Li | 2020 | Plant Phenomics2020，2，1： | 8 |
| 7 | Electrically Programmable Terahertz Diatomic Metamolecules for Chiral Optical Control显示文摘Optical chirality is central to many industrial photonic technologies including enantiomer identifcation,ellipsometry-based tomography,and spin multiplexing in optical communications.However,a substantial chiral response requires a threedimensional constituent,thereby making the morphology highly complex to realize structural reconfguration.Moreover,an active reconfguration demands intense dosage of external stimuli that pose a major limitation for on-chip integration.Here,we report a low bias,electrically programmable synthetic chiral paradigm with a remarkable reconfguration among levorotatory,dextrorotatory,achiral,and racemic conformations.Te switchable optical activity induced by the chiral conformations enables a transmission-type duplex spatial light modulator for terahertz single pixel imaging.Te prototype delivers a new strategy towards reconfgurable stereoselective photonic applications and opens up avenues for on-chip programmable chiral devices with tremendous applications in biology,medicine,chemistry,and photonics. | Longqing Cong Prakash Pitchappa Nan Wang Ranjan Singh | 2019 | Research2019，，1： | 8 |
| 8 | Deep-learning-powered photonic analogto-digital conversion显示文摘Analog-to-digital converters(ADCs)must be high speed,broadband,and accurate for the development of modern information systems,such as radar,imaging,and communications systems;photonic technologies are regarded as promising technologies for realizing these advanced requirements.Here,we present a deep-learning-powered photonic ADC architecture that simultaneously exploits the advantages of electronics and photonics and overcomes the bottlenecks of the two technologies,thereby overcoming the ADC tradeoff among speed,bandwidth,and accuracy.Via supervised training,the adopted deep neural networks learn the patterns of photonic system defects and recover the distorted data,thereby maintaining the high quality of the electronic quantized data succinctly and adaptively.The numerical and experimental results demonstrate that the proposed architecture outperforms state-ofthe-art ADCs with developable high throughput;hence,deep learning performs well in photonic ADC systems.We anticipate that the proposed architecture will inspire future high-performance photonic ADC design and provide opportunities for substantial performance enhancement for the next-generation information systems. | Shaofu Xu Xiuting Zou Bowen Ma Jianping Chen Lei Yu Weiwen Zou | 2019 | Light(Science & Applications)2019，8，1： | 8 |
| 9 | Circular RNA circStag1 promotes bone regeneration by interacting with HuR显示文摘Postmenopausal osteoporosis is a common bone metabolic disorder characterized by deterioration of the bone microarchitecture,leading to an increased risk of fractures.Recently,circular RNAs(circ RNAs)have been demonstrated to play pivotal roles in regulating bone metabolism.However,the underlying functions of circ RNAs in bone metabolism in postmenopausal osteoporosis remain obscure.Here,we report that circ Stag1 is a critical osteoporosis-related circ RNA that shows significantly downregulated expression in osteoporotic bone marrow mesenchymal stem cells(BMSCs)and clinical bone tissue samples from patients with osteoporosis.Overexpression of circ Stag1 significantly promoted the osteogenic capability of BMSCs.Mechanistically,we found that circ Stag1 interacts with human antigen R(Hu R),an RNA-binding protein,and promotes the translocation of Hu R into the cytoplasm.A high cytoplasmic level of Hu R led to the activation of the Wnt signaling pathway by stabilizing and enhancing low-density lipoprotein receptor-related protein 5/6(Lrp5/6)andβ-catenin expression,thereby stimulating the osteogenic differentiation of BMSCs.Furthermore,overexpression of circ Stag1 in vivo by circ Stag1-loaded adeno-associated virus(circ Stag1-AAV)promoted new bone formation,thereby preventing bone loss in ovariectomized rats.Collectively,we show that circ Stag1 plays a pivotal role in promoting the regeneration of bone tissue via Hu R/Wnt signaling,which may provide new strategies to prevent bone metabolic disorders such as postmenopausal osteoporosis. | Gaoyang Chen Canling Long Shang Wang Zhenmin Wang Xin Chen Wanze Tang Xiaoqin He Zhiteng Bao Baoyu Tan Jin Zhao Yongheng Xie Zhizhong Li Dazhi Yang Guozhi Xiao Songlin Peng | 2022 | Bone Research2022，10，3： | 7 |
| 10 | A basic helix-loop-helix transcription factor, PhFBH4, regulates flower senescence by modulating ethylene biosynthesis pathway in petunia显示文摘The basic helix-loop-helix(bHLH)transcription factors(TFs)play important roles in regulating multiple biological processes in plants.However,there are few reports about the function of bHLHs in flower senescence.In this study,a bHLH TF,PhFBH4,was found to be dramatically upregulated during flower senescence.Transcription of PhFBH4 is induced by plant hormones and abiotic stress treatments.Silencing of PhFBH4 using virus-induced gene silencing or an antisense approach extended flower longevity,while transgenic petunia flowers with an overexpression construct showed a reduction in flower lifespan.Abundance of transcripts of senescence-related genes(SAG12,SAG29)was significantly changed in petunia PhFBH4 transgenic flowers.Furthermore,silencing or overexpression of PhFBH4 reduced or increased,respectively,transcript abundances of important ethylene biosynthesis-related genes,ACS1 and ACO1,thereby influencing ethylene production.An electrophoretic mobility shift assay showed that the PhFBH4 protein physically interacted with the G-box cis-element in the promoter of ACS1,suggesting that ACS1 was a direct target of the PhFBH4 protein.In addition,ectopic expression of this gene altered plant development including plant height,internode length,and size of leaves and flowers,accompanied by alteration of transcript abundance of the gibberellin biosynthesis-related gene GA2OX3.Our results indicate that PhFBH4 plays an important role in regulating plant growth and development through modulating the ethylene biosynthesis pathway. | Jing Yin Xiaoxiao Chang Takao Kasuga Mai Bui Michael S Reid Cai-Zhong Jiang | 2015 | Horticulture Research2015，2，1： | 7 |
| 11 | Rapid, continuous projection multi-photon 3D printing enabled by spatiotemporal focusing of femtosecond pulses显示文摘There is demand for scaling up 3D printing throughput,especially for the multi-photon 3D printing process that provides sub-micrometer structuring capabilities required in diverse fields.In this work,high-speed projection multiphoton printing is combined with spatiotemporal focusing for fabrication of 3D structures in a rapid,layer-by-layer,and continuous manner.Spatiotemporal focusing confines printing to thin layers,thereby achieving print thicknesses on the micron and sub-micron scale.Through projection of dynamically varying patterns with no pause between patterns,a continuous fabrication process is established.A numerical model for computing spatiotemporal focusing and imaging is also presented which is verified by optical imaging and printing results.Complex 3D structures with smooth features are fabricated,with millimeter scale printing realized at a rate above 10−3 mm3 s−1.This method is further scalable,indicating its potential to make fabrications of 3D structures with micro/nanoscale features in a practical time scale a reality. | Paul Somers Zihao Liang Jason E.Johnson Bryan W.Boudouris Liang Pan Xianfan Xu | 2021 | Light(Science & Applications)2021，10，10： | 7 |
| 12 | All-silicon dual-cavity fiber-optic pressure sensor with ultralow pressure-temperature cross-sensitivity and wide working temperature range显示文摘Pressure-temperature cross-sensitivity and its accompanying temperature-related stability is a nerve-wracking obstruction for pressure sensor performance in a wide temperature range.To solve this problem,we propose a novel(to the best of our knowledge)all-silicon dual-cavity optical Fabry–Perot interferometer(FPI)pressure sensor.The all-silicon structure has high intrinsic reflectivity and is able to eliminate the influence of thermal-expansion-mismatch-induced stress and chemical-reaction-induced gas generation,and therefore,in essence,enhances measurement accuracy.From the experiment results,the pressure-temperature cross-sensitivity is reduced to be∼5.96 Pa/℃,which presents the lowest pressure-temperature cross-sensitivity among the FPI pressure sensors with the capability of surviving high temperatures up to 700℃ thereby opening the way for high-precision pressure monitoring in various harsh and remote environments. | Xue Wang Junfeng Jiang Shuang Wang Kun Liu Tiegen Liu | 2021 | Photonics Research2021，9，4： | 7 |
| 13 | The involvement of wheat U-box E3 ubiquitin ligase TaPUB1 in salt stress tolerance显示文摘U-box E3 ubiquitin ligases play important roles in the ubiquitin/26 S proteasome machinery and in abiotic stress responses. Ta PUB1-overexpressing wheat(Triticum aestivum L.) were generated to evaluate its function in salt tolerance. These plants had more salt stress tolerance during seedling and flowering stages, whereas the Ta PUB1-RNA interference(RNAi)-mediated knock-down transgenic wheat showed more salt stress sensitivity than the wild type(WT). Ta PUB1 overexpression upregulated the expression of genes related to ion channels and increased the net root Na+efflux, but decreased the net K+efflux and H+influx, thereby maintaining a low cytosolic Na+/K+ratio, compared with the WT. However,RNAi-mediated knock-down plants showed the opposite response to salt stress. Ta PUB1 could induce the expression of some genes that improved the antioxidant capacity of plants under salt stress. Ta PUB1 also interacted with Ta MP(Triticum aestivum α-mannosidase protein), a regulator playing an important role in salt response in yeast and in plants. Thus, low cytosolic Na+/K+ratios and better antioxidant enzyme activities could be maintained in wheat with overexpression of Ta PUB1 under salt stress. Therefore, we conclude that the U-box E3 ubiquitin ligase Ta PUB1 positively regulates salt stress tolerance in wheat. | Wenlong Wang Wenqiang Wang Yunzhen Wu Qinxue Li Guangqiang Zhang Ruirui Shi Junjiao Yang Yong Wang Wei Wang | 2020 | Journal of Integrative Plant Biology2020，62，5： | 6 |
| 14 | IgD class switch recombination is not controlled through the immunoglobulin heavy chain 3′ regulatory region super-enhancer显示文摘In secondary lymphoid organs,mature B cells express membrane immunoglobulin(Ig)of M and D isotypes(IgM and IgD,respectively)of the same specificity through alternative splicing of a pre-mRNA encompassing the VDJ variable region and Cμand Cδheavy chain constant exons.1 After encountering antigen,B cells undergo class switch recombination(CSR)by which the Cμgene is substituted with Cγ,Cεor Cα,thereby generating IgG,IgE and IgA antibodies of the same antigenic specificity but with new effector functions.CSR requires the DNA-editing enzyme activation-induced deaminase(AID). | Hussein Issaoui Nour Ghazzaui Alexis Saintamand Yves Denizot François Boyer | 2017 | Cellular & Molecular Immunology2017，14，10： | 6 |
| 15 | 随气候变化塔克拉玛干沙漠碳汇能力逐渐衰弱显示文摘全球碳循环中被长期忽视的沙漠生态系统,可能会封存大量CO2发挥碳汇作用.塔克拉玛干沙漠是世界第二大流动沙漠,其碳汇作用的内部过程以及气候变化下碳汇的长期趋势仍不明确.研究发现,流沙的热量波动引起含CO2的土壤空气的膨胀/收缩和盐碱的化学作用共同控制了塔克拉玛干沙漠流沙CO2交换作用.这些过程的相互作用使得该沙漠流沙表现出明显的碳汇作用(速率为1.60×106 t a-1).如果以塔克拉玛干沙漠流沙的CO2吸收量为平均状态,那么全球流动沙漠每年可吸收CO2约2.125×108 t.但是,随气候变化,流沙温差的增大会刺激流沙中空气膨胀,向大气中注入更多的CO2,导致未来流沙碳汇能力逐渐减少.该发现对于评估全球碳循环、缩小碳失汇差额和重新认识沙漠生态系统在碳循环中的地位具有重要作用. | 杨帆 黄建平 周成龙 杨兴华 买买提艾力 买买提依明 李超凡 潘红林 霍文 于海鹏 刘晓岳 郑新倩 韩东亮 何清 孟露 常俊 | 2020 | Science Bulletin2020，65，6： | 6 |
| 16 | Boosting proso millet yield by altering canopy light distribution in proso millet/mung bean intercropping systems显示文摘To elucidate the mechanism by which intercropping proso millet(Panicum miliaceum L.)with mung bean(Vigna radiata L.)increases proso millet yield and to determine how this higher yield results from maximization of resources use efficiency,we designed and conducted four strip intercropping row arrangements,including two rows of proso millet alternating with two rows of mung bean(2P2M),four rows of proso millet alternating with two rows of mung bean(4P2M),four rows of proso millet alternating with four rows of mung bean(4P4M),two rows of proso millet alternating with four rows of mung bean(2P4M),sole proso millet(SP,control)and sole mung bean(SM,control)in Yulin,Shaanxi,China.Photosynthetically active radiation(PAR)in the canopy,radiation use efficiency(RUE),leaf photosynthetic characteristics,dry matter accumulation and allocation,and yield of proso millet were investigated.The results showed that the intercropping systems had higher PAR than the monoculture.Mean PAR intensities were increased by respectively 2.2%–23.4%,19.8%–59.7%,and 61.2%–133.3%in the proso millet upper,middle and lower canopies compared with SP.The increase in PAR directly increased RUE,a result attributed mainly to the increase in photosynthetic capacity,including net photosynthetic rate and chlorophyll content.These responses resulted in increased dry matter allocation to plant organs.Yield of intercropped proso millet was 6.8%–37.3%higher than that under monoculture and the land equivalent ratios for the different intercropping patterns were all greater than unity(>1).In general,yield followed a positive linear function of PAR in the intercropping system.The results indicated that intercropping can boost proso millet yield,evidently by altering light distribution within its canopy and consequently increasing RUE,thereby increasing leaf photosynthetic capacity,dry matter accumulation,and allocation to the grain.The optimum combination for improving the growth and yield of proso millet on the Loess Plateau of China was 2P4M. | Xiangwei Gong Uzizerimana Ferdinand Ke Dang Jing Li Guanghua Chen Yan Luo Pu Yang Baili Feng | 2020 | The Crop Journal2020，8，2： | 6 |
| 17 | MdWRKY15 improves resistance of apple to Botryosphaeria dothidea via the salicylic acid-mediated pathway by directly binding the MdICS1 promoter^FA显示文摘Isochorismate synthase(ICS) plays an essential role in the accumulation of salicylic acid(SA) and plant disease resistance. Diseases caused by Botryosphaeria dothidea affect apple yields. Thus, it is important to understand the role of ICS1 in disease resistance to B. dothidea in apple. In this study, SA treatment enhanced the resistance to B. dothidea. Md ICS1 was induced by B. dothidea and enhanced the resistance to B. dothidea. Md ICS1 promoter analysis indicated that the W-box was vital for the response to B. dothidea treatment. Md WRKY15 was found to interact with the W-box using yeast one-hybrid screening. Subsequently, the interaction was confirmed by EMSA, yeast one-hybrid, Ch IP-PCR, and quantitative PCR assays. Moreover, luciferase and GUS analysis further indicated thatMd ICS1 was transcriptionally activated by Md WRKY15. Finally, we found the function of Md WRKY15 in the resistance to B. dothidea was partially dependent on Md ICS1 from the phenotype of transgenic apples and calli. In summary, we revealed that Md WRKY15 activated the transcription of Md ICS1 by directly binding to its promoter to increase the accumulation of SA and the expression of disease-related genes, thereby resulting in the enhanced resistance to B.dothidea in the SA biosynthesis pathway. | Xian-Yan Zhao Chen-Hui Qi Han Jiang Ming-Shuang Zhong Chun-Xiang You Yuan-Yuan Li Yu-Jin Hao | 2020 | Journal of Integrative Plant Biology2020，62，4： | 5 |
| 18 | A naturally occurring variation in the BrMAM-3 gene is associated with aliphatic glucosinolate accumulation in Brassica rapa leaves显示文摘Glucosinolate profiles significantly vary among Brassica rapa genotypes.However,the molecular basis of these variations is largely unknown.In this study,we investigated a major quantitative trait locus(QTL)controlling aliphatic glucosinolate accumulation in B.rapa leaves.The QTL,which encompasses three tandem MAM genes and two MYB genes,was detected in two BC2DH populations.Among the five-candidate genes,only the expression level of BrMAM-3(Bra013007)was significantly correlated with the accumulation of aliphatic glucosinolates in B.rapa leaves.We identified a naturally occurring insertion within exon 1 of BrMAM-3,which is predicted to be a loss-of-function mutation,as confirmed by qRT-PCR.We determined that the loss of function was associated with the low glucosinolate content in B.rapa accessions.Furthermore,overexpressing the BrMAM-3 gene resulted in an increase in total aliphatic glucosinolates in Arabidopsis transgenic lines.Our study provides insights into the molecular mechanism underlying the accumulation of aliphatic glucosinolates in B.rapa leaves,thereby facilitating in the manipulation of total aliphatic glucosinolate content in Brassica crops. | Jifang Zhang Hui Wang Zhiyuan Liu Jianli Liang Jian Wu Feng Cheng Shiyong Mei Xiaowu Wang | 2018 | Horticulture Research2018，5，1： | 5 |
| 19 | Melatonin alters the secondary metabolite profile of grape berry skin by promoting VvMYB14-mediated ethylene biosynthesis显示文摘The interplay between melatonin and ethylene in the regulation of fruit metabolism and the underlying molecular mechanism of this interplay remain largely unclear.Here,widely targeted metabolomics analysis revealed a total of 464 metabolites present in berry skin.Among them,27 significantly differentially accumulated metabolites(DAMs)were produced in response to melatonin treatment in the presence or absence of 1-MCP.Most of the DAMs were secondary metabolites,including flavonoids,phenolic acids,stilbenes,and flavonols.Additionally,the accumulation of 25 DAMs was regulated by melatonin via ethylene.RNA-seq analysis indicated that melatonin primarily regulated the pathways of plant hormone signal transduction and secondary metabolite biosynthesis via ethylene.Gene-metabolite association analysis showed that melatonin regulated the expression of the VvSTS1,VvF3H,VvLAR2,and VvDFR genes,suggesting that these genes may play key roles in regulating secondary metabolites in the skin;additionally,VvMYB14 and VvACS1 were suggested to be involved in the regulation of secondary metabolites.Further experiments revealed that melatonin induced the expression of VvMYB14 and that VvMYB14 increased ethylene production by transcriptionally activating VvACS1,thereby affecting the accumulation of secondary metabolites.Collectively,melatonin promotes ethylene biosynthesis and alters secondary metabolite accumulation through the regulation of VvACS1 by VvMYB14. | Wanyun Ma Lili Xu Shiwei Gao Xingning Lyu Xiaolei Cao Yuxin Yao | 2021 | Horticulture Research2021，8，1： | 4 |
| 20 | CPPU may induce gibberellin-independent parthenocarpy associated with PbRR9 in‘Dangshansu’pear显示文摘Parthenocarpy is a valuable trait in self-incompatible plants,such as pear.N-(2-chloro-4-pyridyl)-N’-phenylurea(CPPU),a synthetic cytokinin analog,can induce parthenocarpy in pear(Pyrus spp.),but the mechanism of induction is unclear.To investigate the role of gibberellin in CPPU-induced parthenocarpy in pear,CPPU supplemented with paclobutrazol(PAC)was sprayed onto‘Dangshansu’pear.We found that the fruit set rate of pear treated with CPPU supplemented with PAC was identical to that in a CPPU-alone treatment group.In regard to cell development,CPPU mainly promoted hypanthium cell division and expansion,and PAC application had no influence on CPPU-induced cell development.RNA sequencing revealed that gibberellin 20 oxidase and gibberellin 3 oxidase genes were not differentially expressed following CPPU treatment.According to the analysis of fruit phytohormone content,the CPPU treatments did not induce gibberellin biosynthesis.These results suggest that CPPU-induced parthenocarpy may be gibberellin independent in‘Dangshansu’pear.After CPPU treatment,the indole acetic acid(IAA)content in fruit was significantly increased,and the abscisic acid(ABA)content was significantly decreased.Similarly,RNA sequencing revealed that many genes involved in the auxin and ABA pathways were significantly differentially expressed in the CPPU treatment groups;among them,indole-3-pyruvate monooxygenase(YUCCA)was significantly upregulated and 9-cis-epoxycarotenoid dioxygenase(NCED)was significantly downregulated.IAA and ABA may thus play important roles in CPPU-induced parthenocarpy.PbTwo-component response regulator9(PbRR9),PbYUCCA4,and PbNCED6 were then selected to further elucidate the mechanism of CPPU-induced parthenocarpy.A yeast one-hybrid assay indicated that PbRR9 can combine with the PbYUCCA4 and PbNCED6 promoters.Dual luciferase assays revealed that PbRR9 can promote and repress the activities of the PbYUCCA4 and PbNCED6 promoters,respectively.After the transient expression of PbRR9 in fruits,PbYUCCA4 expression was significantly upregulated,and PbNCED6 expression was significantly downregulated.This study uncovered a CPPU-induced parthenocarpy mechanism that is different from that in tomato.CPPU may upregulate PbYUCCA4 and downregulate PbNCED6 by upregulating PbRR9,thereby increasing IAA content and decreasing ABA content to ultimately induce parthenocarpy in‘Dangshansu’pear.However,because only a single time point was used and because‘botanical’and‘accessory’fruits have different structures,this conclusion is still preliminary. | Liu Cong Ting Wu Hanting Liu Huibin Wang Haiqi Zhang Guangping Zhao Yao Wen Qianrong Shi Lingfei Xu Zhigang Wang | 2020 | Horticulture Research2020，7，1： | 4 |
您所在的机构暂未开通此部分的功能，请联系管理员