A novel nonviral nanoparticle gene vector: Poly-L-lysine- silica nanoparticles

查看全文 作　　者：Shiguo [1]Zhu;Hongbin [1]Lu;Juanjuan [1]Xiang;Ke [1]Tang;Bicheng [1]Zhang;Ming [1]Zhou;Chen [1]Tan;Guiyuan [1]Li

机构地区：[1]Cent S Univ, Xiangya Sch Med, Inst Canc Res, Changsha 410078, Peoples R China

出　　处：《Chinese Science Bulletin》索引2002年第47卷第8期,共5页

基　　金：This work was supported by the National High Technology R&D Project of China (Grant No. 102-10-01-05);the Special Funds for Major State Basic Research of China (Grant No. 1998051008);the Key Program Funds of the Ministry of Education (Grant No. 2000-15 摘　　要：DNA delivery is a core technology for gene structure and function research as well as clinical settings. The ability to safely and efficiently targeted transfer foreign DNA into cells is a fundamental goal in biotechnology. With the development of nanobiotechnology, nanoparticle gene vectors brought about new hope to reach the goal. In our research, silica nanoparticles (SiNP) were synthesized first in a microemulsion system polyoxyethylene nonylphenyl ether (OP-10)/cyclohexane/ammonium hydroxide, at the same time the effects of SiNP size and its distribution were elucidated by orthogonal analysis; then poly-L-lysine (PLL) was linked on the surface of SiNP by nanoparticle surface energy and electrostatically binding; lastly a novel complex nanomate-rial-poly-L-lysine-silica nanoparticles (PLL-SiNP) wasprepared. The analysis of plasmid DNA binding and DNase I enzymatic degradation discovered that PLL-SiNP could bind DNA, and protect it against enzymatic degradation. Cell transfection showed that 关键词：NANOPARTICLE gene vector SILICA NANOPARTICLES poly-L-lysine-silica NANOPARTICLES synthesis DNA delivery cell transfection.

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A novel nonviral nanoparticle gene vector: Poly-L-lysine- silica nanoparticles

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