A Study of the Technique of Western Blot for Diagnosis of Lyme Disease caused by Borrelia afzelii in China

查看全文 作　　者：LIU Zhi [1,2,3]Yun;HAO [1]Qin;HOU Xue [1]Xia;JIANG [1]Yi;GENG [1]Zhen;WU Yi [2]Mou;WAN Kang [1]Lin

机构地区：[1]State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention;[2]Institute of Pathogenic Biology,University of South China;[3]YuShui District Center for Disease Control and Prevention

出　　处：《Biomedical and Environmental Sciences》索引2013年第26卷第3期,共11页

基　　金：supported by the 12th Five-Year Major National Science and Technology Projects of China (No.2011ZX10004-001);Natural Science Foundation of China (31100105) 摘　　要：Objective To study the technique of Western blot for the diagnosis of Lyme disease caused by Borrelia afzelii in China and to establish the standard criteria by operational procedure. Methods FP1, which is the representative strain of B. afzelii in China, was analyzed by SDS‐PAGE, electro transfer and immunoblotting assays. The molecular weights of the protein bands of FP1 were analyzed by Gel‐Pro analysis software. In a study using 451 serum samples (159 patients with Lyme disease and 292 controls), all observed bands were recorded. The accuracy of the WB as a diagnostic test was established by using the ROC curve and Youden index. Results Criteria for a positive diagnosis of Lyme disease were established as at least one band of P83/100, P58, P39, OspB, OspA, P30, P28, OspC, P17, and P14 in the IgG test and at least one band of P83/100, P58, P39, OspA, P30, P28, OspC, P17, and P41 in the IgM test. For IgG criteria, the sensitivity, specificity and Youden index were 69.8%, 98.3%, and 0.681, respectively; for IgM criteria, the sensitivity, specificity and Youden index were 47%, 94.2%, and 0.412, respectively. Conclusion Establishment of WB criteria for B. afzelii is important in validating the diagnostic assays for Lyme disease in China. 关键词：抗体检测诊断技术莱姆病中国 BLOT SDS-PAGE 诊断测试 ROC曲线

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A Study of the Technique of Western Blot for Diagnosis of Lyme Disease caused by Borrelia afzelii in China

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