Function of lncRNAs and approaches to lncRNA-protein interactions

查看全文 作　　者：ZHU [1,2]JuanJuan;FU [1]HanJiang;WU [2]YongGe;ZHENG [1]XiaoFei

机构地区：[1]Institute of Radiation Medicine,Academy of Military Medical Sciences;[2]College of Life Sciences,Jilin University

出　　处：《Science China(Life Sciences)》索引2013年第56卷第10期,共10页

基　　金：supported by the National Basic Research Program of China(2010CB912801,2013CB910801);National High Technology Research and Development Program of China(2012AA022501);National Natural Science Foundation of China(31070702,31270836) 摘　　要：Long non-coding RNAs(lncRNAs),which represent a new frontier in molecular biology,play important roles in regulating gene expression at epigenetic,transcriptional and post-transcriptional levels.More and more lncRNAs have been found to play important roles in normal cell physiological activities,and participate in the development of varieties of tumors and other diseases.Previously,we have only been able to determine the function of lncRNAs through multiple mechanisms,including genetic imprinting,chromatin remodeling,splicing regulation,mRNA decay,and translational regulation.Application of technological advances to research into the function of lncRNAs is extremely important.The major tools for exploring lncRNAs include microarrays,RNA sequencing(RNA-seq),Northern blotting,real-time quantitative reverse transcription-polymerase chain reaction(qRT-PCR),fluorescence in situ hybridization(FISH),RNA interference(RNAi),RNA-binding protein immunoprecipitation(RIP),chromatin isolation by RNA purification(ChIRP),crosslinking-immunopurification(CLIP),and bioinformatic prediction.In this review,we highlight the functions of lncRNAs,and advanced methods to research lncRNA-protein interactions. 关键词：蛋白相互作用 Northern 定量RT-PCR RNA结合蛋白蛋白质相互作用转录后水平染色质重塑 RNAS

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Function of lncRNAs and approaches to lncRNA-protein interactions

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